Capillarisin, as a constituent from Artemisiae Capillaris herba, is found to exert anti-inflammatory and antioxidant properties. Capillarisin can be used for the research of asthma-mediated complications and can be a potential neuroprotectant against bupivacaine-induced neurotoxicity[1][2][3].
体外研究 (In Vitro)
Capillarisin (0~40 μM; 24 hours; SH-SY5Y cells) does not produce any significant changes on the viability of SH-SY5Y cells[3]. . Capillarisin (40 μM; 24 hours; SH-SY5Y cells) induces PI3K/PKB pathway inactivation, which inhibiting apoptosis in bupivacaine-challenged SH-SY5Y cells is overturned by LY294002 treatment and counteracts bupivacaine-induced injury via activating the PI3K/PKB pathway[3]. . Capillarisin antagonizes bupivacaine-induced oxidative stress via activating the PI3K/PKB pathway in SH-SY5Y cells. Capillarisin inhibits bupivacaine-induced mitochondrial injury and endoplasmic reticulum stress via activating PI3K/PKB pathway[3].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay[3]
Cell Line:
SH-SY5Y cells
Concentration:
0~40 μM
Incubation Time:
24 hours
Result:
Did not produce any significant changes on the viability of SH-SY5Y cells.
Western Blot Analysis[3]
Cell Line:
SH-SY5Y cells
Concentration:
40 μM
Incubation Time:
24 hours
Result:
Induced PI3K/PKB pathway inactivation in SH-SY5Y cells.
Apoptosis Analysis[3]
Cell Line:
SH-SY5Y cells
Concentration:
40 μM
Incubation Time:
24 hours
Result:
Induced inhibition of apoptosis in bupivacaine-challenged SH-SY5Y cells was overturned by LY294002 treatment.
体内研究 (In Vivo)
Capillarisin (20 and 80 mg/kg; i.p.; 1 hour) pretreatment strongly inhibits NF-κB mediated genes (iNOS, COX-2)[4]. Capillarisin significantly reduces the plasma leading nitrite production. Capillarisin markedly suppresses the adenosine 5′-triphosphate (ATP) in plasma and substance P in CFA-induced paw tissue[4].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献
[1]. Peng G, et al. Capillarisin exerts antiasthmatic activity in neonatal rats via modulating the matrix remodeling. Pak J Pharm Sci. 2020;33(4(Supplementary)):1907-1915.
[2]. Komiya T, et al. Capillarisin, a Constituent from Artemisiae Capillaris Herba. Chemical and Pharmaceutical Bulletin, 1975
[3]. Zhao T, Wang Q. Capillarisin protects SH-SY5Y cells against bupivacaine-induced apoptosis via ROS-mediated PI3K/PKB pathway. Life Sci. 2020;259:118279.
[4]. Khan S, et al. Anti-hyperalgesic and anti-allodynic activities of capillarisin via suppression of inflammatory signaling in animal model. J Ethnopharmacol. 2014;152(3):478-486.
