Polyphyllin C

天然产物 糖类和糖苷 Saccharides and Glycosides

Polyphyllin C;

Polyphyllin C (compound 2) 是一种甾烷型甾体皂苷。Polyphyllin C 表现出轻度的酪氨酸酶抑制活性 (IC50=36.87 µM) 和中度抗利什曼原虫 (IC50=1.59 µg/mL) 活性。

Polyphyllin C

Polyphyllin C Chemical Structure

CAS No. : 76296-71-4

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生物活性

Polyphyllin C (compound 2) is a spirostanol saponin. Polyphyllin C exhibits mild (IC50=36.87 µM) activities against the tyrosinase and moderate (IC50=1.59 µg/mL) antileishmanial activities[1][2].

IC50 Target

IC50: 36.87 µM (tyrosinase)[1]

分子量

722.90

Formula

C39H62O12

CAS 号

76296-71-4

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Devkota KP, et, al. Tyrosinase inhibitory and antileishmanial constituents from the rhizomes of Paris polyphylla. Nat Prod Res. 2007 Apr;21(4):321-7.

    [2]. Singh SB, et, al. Spirostanol saponins from Paris polyphylla, structures of polyphyllin C, D, E and F. Phytochemistry.1980; 21(12): 2925-2929.

6-Methoxytricin(Synonyms: 6-甲氧基哌啶)

天然产物 黄酮类 Flavonoids

6-Methoxytricin (Synonyms: 6-甲氧基哌啶)

6-Methoxytricin (Compound 6) 6-甲氧基哌啶是从艾蒿 Artemisia iwayomogi 分离的类黄酮。6-Methoxytricin (Compound 6) 是醛糖还原酶 (AR) 和晚期糖基化终产物 (AGE) 的抑制剂,IC50 值分别为 30.29 μM 和 134.88 μM。6-Methoxytricin (Compound 6) 具有抗糖尿病并发症的潜力。

6-Methoxytricin(Synonyms: 6-甲氧基哌啶)

6-Methoxytricin Chemical Structure

CAS No. : 76015-42-4

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5 mg ¥3430 询问价格 & 货期

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生物活性

6-Methoxytricin (Compound 6) is an flavonoid isolated from Artemisia iwayomogi. 6-Methoxytricin (Compound 6) is an inhibitor on aldose reductase (AR) and advanced glycation endproduct (AGE) formation activities with IC50 values of 30.29 μM and 134.88 μM, respectively. 6-Methoxytricin (Compound 6) has potential as an anti-diabetic complications agent[1].

IC50 & Target

IC50: 30.29 μM (AR); 134.88 μM (AGE) (6-​Methoxytricin)[1]

分子量

360.31

Formula

C18H16O8

CAS 号

76015-42-4

中文名称

6-甲氧基哌啶

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Lee YK, et al. Inhibitory Effect of Chemical Constituents Isolated from Artemisia iwayomogi on Polyol Pathway and Simultaneous Quantification of Major Bioactive Compounds. Biomed Res Int. 2017;2017:7375615.

Ginsenoside C-K(Synonyms: 人参皂苷 C-K; Ginsenoside compound K; Ginsenoside K)

天然产物 糖类和糖苷 Saccharides and Glycosides

Ginsenoside C-K;(Synonyms: 人参皂苷 C-K; Ginsenoside compound K; Ginsenoside K) 纯度: 98.04%

Ginsenoside C-K 是一种 Ginsenoside Rb1 的细菌代谢物。Ginsenoside C-K 通过抑制诱生型一氧化氮合酶 (iNOS) 和 COX-2 来发挥抗炎作用。在人肝微粒体中,Ginsenoside C-K 抑制 CYP2C9CYP2A6 活性,IC50 分别为 32.0±3.6 μM和 63.6±4.2 μM。

Ginsenoside C-K(Synonyms: 人参皂苷 C-K; Ginsenoside compound K;  Ginsenoside K)

Ginsenoside C-K Chemical Structure

CAS No. : 39262-14-1

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ¥1028 In-stock
5 mg ¥750 In-stock
10 mg ¥1350 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

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Ginsenoside C-K 相关产品

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  • Natural Product Library Plus
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  • Immunology/Inflammation Compound Library
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  • Natural Product Library
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  • Medicine Food Homology Compound Library
  • Terpenoids Library
  • Pyroptosis Compound Library
  • Traditional Chinese Medicine Monomer Library
  • Neuroprotective Compound Library
  • Anti-Pancreatic Cancer Compound Library
  • Microbial Metabolite Library
  • Angiogenesis Related Compound Library
  • Anti-Colorectal Cancer Compound Library

生物活性

Ginsenoside C-K, a bacterial metabolite of G-Rb1, exhibits anti-inflammatory effects by reducing iNOS and COX-2. Ginsenoside C-K exhibits an inhibition against the activity of CYP2C9 and CYP2A6 in human liver microsomes with IC50s of 32.0±3.6 μM and 63.6±4.2 μM, respectively.

IC50 Target[1][4]

COX-2

;

iNOS

;

CYP2C9

32 mu;M (IC50)

CYP2A6

63.6 mu;M (IC50)

体外研究
(In Vitro)

Ginsenoside C-K, a bacterial metabolite of G-Rb1, exhibits anti-inflammatory effects mainly by reducing inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, and proinflammatory cytokines. Ginsenoside C-K suppresses the expression of proinflammatory cytokines by downregulating the activities of IRAK-1, MAPKs, IKK-α, and NF-κB in LPS-treated murine peritoneal macrophages. Ginsenoside C-K also suppresses the expression of iNOS and COX-2 by inhibiting NF-κB signaling in LPS-stimulated RAW264.7 cells. In zymosan-treated bone-marrow-derived macrophages (BMDMs) and RAW264.7 cells, Ginsenoside C-K inhibits inflammatory responses by negatively regulating the secretion of proinflammatory cytokines, the activation of MAPKs, and the generation of ROS. In addition, anti-inflammatory activity of Ginsenoside C-K has been observed in LPS-stimulated microglial cells. Ginsenoside C-K hinders inflammatory responses by controlling both the generation of ROS and the activities of MAPKs, NF-κB, and AP-1[1]. Ginsenoside C-K, a major metabolite of ginsenosides in the gastrointestinal tract, inhibits NF-κB signaling in a PXR-dependent manner. Ginsenoside C-K is shown to promote recovery of dextran sulfate sodium (DSS) -induced colitis by suppressing NF-κB activation. Ginsenoside C-K significantly reduces TNF-α-induced upregulation of IL-1β and iNOS mRNA levels, and restores the mRNA levels of PXR and CYP3A4 in LS174T cells[2]. Ginsenoside C-K, one of the intestinal metabolites of 20(S)-protopanaxadiol derivatives, exhibits an inhibition against the activity of CYP2C9 in human liver microsomes with an IC50 value of 32.0±3.6 μM, a weak inhibition against the activity of CYP2A6 in human liver microsomes with an IC50 value of 63.6±4.2 μM, and an even weaker inhibition against the activity of CYP2D6 in human liver microsomes with an IC50 value of more than 100 μM[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

The weight of the collagen-induced arthritis (CIA) mice increases slowly and is significantly less than that of the normal DBA/1 mice beginning on d 3 after injection of the emulsion. Ginsenoside C-K (28, 56, and 112 mg/kg) mice recover their weight by d 32 after the emulsion injection. Ginsenoside C-K (56 and 112 mg/kg) and Methotrexate (MTX)-treated (2 mg/kg) mice show significantly increased body weight on d 50 as compared with CIA mice. Hind paw-swelling began on d 24 post-immunization. CIA mice are treated from d 28 to d 50. Arthritis scores are measured every 4 d beginning on d 24. Ginsenoside C-K (56 and 112 mg/kg) significantly reduces the arthritis scores of the mice on d 51[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

622.87

Formula

C36H62O8

CAS 号

39262-14-1

中文名称

人参皂苷 C-K

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20deg;C 3 years
4deg;C 2 years
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

DMSO : ≥ 100 mg/mL (160.55 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.6055 mL 8.0274 mL 16.0547 mL
5 mM 0.3211 mL 1.6055 mL 3.2109 mL
10 mM 0.1605 mL 0.8027 mL 1.6055 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.75 mg/mL (4.42 mM); Clear solution

    此方案可获得 ≥ 2.75 mg/mL (4.42 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 27.5 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.75 mg/mL (4.42 mM); Clear solution

    此方案可获得 ≥ 2.75 mg/mL (4.42 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 27.5 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂:;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.75 mg/mL (4.42 mM); Clear solution

    此方案可获得 ≥ 2.75 mg/mL (4.42 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 27.5 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Kim JH, et al. Role of ginsenosides, the main active components of Panax ginseng, in inflammatory responsesand diseases. J Ginseng Res. 2017 Oct;41(4):435-443.

    [2]. Liu Y, et al. Ginsenoside metabolites, rather than naturally occurring ginsenosides, lead to inhibition of human cytochrome P450 enzymes. Toxicol Sci. 2006 Jun;91(2):356-64.

    [3]. Zhang J, et al. Ginsenosides Regulate PXR/NF-κB Signaling and Attenuate Dextran Sulfate Sodium-Induced Colitis. Drug Metab Dispos. 2015 Aug;43(8):1181-9.

    [4]. Liu KK, et al. Ginsenoside compound K suppresses the abnormal activation of T lymphocytes in mice with collagen-induced arthritis. Acta Pharmacol Sin. 2014 May;35(5):599-612.

Cell Assay
[2]

LS174T cells are seeded in cell imaging dish. After overnight incubation, cells are treated with ginseng saponin extract (GSE) (100 μg/mL), Rb1 (10 μM), or Ginsenoside C-K (10 μM) for 3 hours, followed by an additional incubation with or without TNF-α (20 ng/mL) for 6 hours. At the end of the incubation, cells are harvested and fixed with 4% paraformaldehyde solution at 20°C for 20 minutes. After washing in PBS, cells are permeabilized with 0.2% Triton X-100 in PBS at room temperature for 5 minutes. After incubation in blocking buffer containing 0.1% Triton X-100 and 5% bovine serum albumin, cells are incubated with rabbit NF-κB p65 antibody at 4°C overnight and then with Alexa Fluor 488-conjugated anti-rabbit IgG antibody at room temperature for 30 minutes in 1% bovine serum albumin in PBS. Fluorescence photographs are obtained using a Zeiss 710 confocal microscope[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[3]

Mice[3]
Specific pathogen-free DBA/1 mice (male, 18±2 g) are used. DBA/1 mice are injected intradermally twice with 0.1 mL of this emulsion (containing 100 mg of chicken type II collagen (CII)/mouse) in the back and the base of the tail. The day of the first immunization is defined as d 0, and the booster injection is administered into the back on d 21. After the onset of arthritis, animals are randomly divided into five groups, and each experimental group consists of ten mice. Mice with CIA are intragastrically administered Ginsenoside C-K (28, 56, or 112 mg/kg) once per day or MTX (2 mg/kg) once every 3 d from d 28 to d 51 after immunization. Normal and CIA mice are administered an equal volume of vehicle (CMC-Na) at the same time[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Kim JH, et al. Role of ginsenosides, the main active components of Panax ginseng, in inflammatory responsesand diseases. J Ginseng Res. 2017 Oct;41(4):435-443.

    [2]. Liu Y, et al. Ginsenoside metabolites, rather than naturally occurring ginsenosides, lead to inhibition of human cytochrome P450 enzymes. Toxicol Sci. 2006 Jun;91(2):356-64.

    [3]. Zhang J, et al. Ginsenosides Regulate PXR/NF-κB Signaling and Attenuate Dextran Sulfate Sodium-Induced Colitis. Drug Metab Dispos. 2015 Aug;43(8):1181-9.

    [4]. Liu KK, et al. Ginsenoside compound K suppresses the abnormal activation of T lymphocytes in mice with collagen-induced arthritis. Acta Pharmacol Sin. 2014 May;35(5):599-612.

DPDPE

DPDPE;

DPDPE 是一种阿片肽,是一种具有抗惊厥作用的选择性阿片受体 (DOR) 激动剂。

DPDPEamp;;

DPDPE Chemical Structure

CAS No. : 88373-73-3

规格 价格 是否有货
1 mg ¥1000 询问价格 货期
5 mg ¥2800 询问价格 货期

* Please select Quantity before adding items.

DPDPE 的其他形式现货产品:

DPDPE TFA

DPDPE 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • GPCR/G Protein Compound Library
  • Neuronal Signaling Compound Library
  • Neurotransmitter Receptor Compound Library

生物活性

DPDPE, an opioid peptide, is a selective δ-opioid receptor (DOR) agonist with anticonvulsant effects[1].

分子量

645.79

Formula

C30H39N5O7S2

CAS 号

88373-73-3

Sequence

Tyr-{Pen}-Gly-Phe-{Pen} (Disulfide bridge:Pen2-Pen5)

Sequence Shortening

Y{Pen}GF{Pen} (Disulfide bridge:Pen2-Pen5)

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
参考文献
  • [1]. F C Tortella, et al. Anticonvulsant Effects of Mu (DAGO) and Delta (DPDPE) Enkephalins in Rats. Peptides