标签归档：compstatin

Compstatin TFA

发表于由

Compstatin TFA; 纯度: 99.46%

Compstatin TFA 是一个 13 残基环肽，是补体系统 C3 的有效抑制剂，具有物种特异性。Compstatin TFA 结合到狒狒的 C3，并对狒狒血液中的蛋白水解裂解具有抗性 （类似人类）。Compstatin TFA 只抑制灵长类补体系统的激活。Compstatin TFA 对补体经典途径和旁路途径的 IC50 值分别为 63 μM 和 12 μM。

Compstatin TFA 

Compstatin TFA Chemical Structure

规格 价格 是否有货 数量
1 mg ￥1100 In-stock
5 mg ￥2700 In-stock
10 mg ; 询价 ;
50 mg ; 询价 ;

* Please select Quantity before adding items.

Compstatin TFA 相关产品

•相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Compstatin TFA, a 13-residue cyclic peptide, is a potent inhibitor of the complement system C3 with species specificity. Compstatin TFA binds to baboon C3 and is resistant to proteolytic cleavage in baboon blood (similar to humans). Compstatin TFA inhibits only the activation of primates’ complement system. Compstatin TFA exhibits IC50 values of 63 μM and 12 μM for classical and alterative complement pathway, respectively[1][2][3].

体外研究
(In Vitro)

Compstatin exhibits an in vitro half-life in human blood of about 2 hr[2].
In solution, compstatin forms a β-turn at residues Gln-5–Gly-8 with the disulfide bridge Cys-2–Cys12, residues Ile-1–Val-4, and Thr-13, forming a hydrophobic cluster[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究
(In Vivo)

Compstatin (21 mg/kg) produces complete inhibition when given as a combination of bolus injection and infusion. Compstatin completely inhibits in vivo heparin/protamine-induced complement activation without adverse effects on heart rate or systemic arterial, central venous, and pulmonary arterial pressures[1].
Compstatin is stable in baboon plasma for more than 24 h[1].
Pig xenografts survival is significantly longer in the Compstatin perfused group than in the control group[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Juvenile baboons (P. Anubis) weighing 10.5-28.8 kg[1].
Dosage: 50, 25 mg/kg 60 min after heparin and 2 min before protamine.
Administration: A bolus injection.
Result: Completely inhibited complement activation induced by heparin–protamine complexes.

分子量

1664.79

Formula

C68H100F3N23O19S2
Sequence Shortening

ICVVQDWGHHRCT-NH2 (Disulfide bridge: Cys2-Cys12)
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Protect from light, stored under nitrogen

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
溶解性数据
In Vitro:;

DMSO : 110 mg/mL (66.07 mM; Need ultrasonic)

H2O : 50 mg/mL (30.03 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.6007 mL 3.0034 mL 6.0068 mL
5 mM 0.1201 mL 0.6007 mL 1.2014 mL
10 mM 0.0601 mL 0.3003 mL 0.6007 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.75 mg/mL (1.65 mM); Clear solution

    此方案可获得 ≥ 2.75 mg/mL (1.65 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 27.5 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.75 mg/mL (1.65 mM); Clear solution

    此方案可获得 ≥ 2.75 mg/mL (1.65 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 27.5 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
  • 3.

    请依序添加每种溶剂：;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.75 mg/mL (1.65 mM); Clear solution

    此方案可获得 ≥ 2.75 mg/mL (1.65 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 27.5 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Soulika AM, et al. Inhibition of heparin/protamine complex-induced complement activation by Compstatin in baboons. Clin Immunol. 2000 Sep;96(3):212-21.

    [2]. Fiane AE, et al. Compstatin, a peptide inhibitor of C3, prolongs survival of ex vivo perfused pig xenografts. Xenotransplantation. 1999 Feb;6(1):52-65.

    [3]. Bert J C Janssen, et al. Structure of compstatin in complex with complement component C3c reveals a new mechanism of complement inhibition. J Biol Chem. 2007 Oct 5;282(40):29241-7.

    [4]. A Sahu, et al. Inhibition of human complement by a C3-binding peptide isolated from a phage-displayed random peptide library. J Immunol. 1996 Jul 15;157(2):884-91.

Compstatin control peptide(Synonyms: 坎普他汀对照肽)

发表于由

Compstatin control peptide;(Synonyms: 坎普他汀对照肽) 纯度: 99.97%

Compstatin control peptide 是一种补体蛋白 C3 (complement C3) 抑制剂，其结合并抑制补体 C3 的裂解。

Compstatin control peptideamp;;(Synonyms: 坎普他汀对照肽)

Compstatin control peptide Chemical Structure

CAS No. : 301544-78-5

规格 价格 是否有货 数量
1 mg ￥2300 In-stock
5 mg ￥6900 In-stock
10 mg ￥11000 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Compstatin control peptide 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Compstatin control peptide is a complement protein C3 inhibitor that binds and inhibits cleavage of complement C3[1].

分子量

1488.67

Formula

C66H101N23O17
CAS 号

301544-78-5
Sequence Shortening

IAVVQDWGHHRAT-NH2
中文名称

坎普他汀对照肽
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

H2O : 25 mg/mL (16.79 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.6717 mL 3.3587 mL 6.7174 mL
5 mM 0.1343 mL 0.6717 mL 1.3435 mL
10 mM 0.0672 mL 0.3359 mL 0.6717 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Ronald D Gorham Jr, et al. Novel compstatin family peptides inhibit complement activation by drusen-like deposits in human retinal pigmented epithelial cell cultures. Exp Eye Res. 2013 Nov;116:96-108.

Compstatin control peptide TFA

发表于由

Compstatin control peptide TFA;

Compstatin control peptide TFA 是一种补体 (complement) 抑制剂，其结合并抑制补体 C3 的裂解。

Compstatin control peptide TFAamp;;

Compstatin control peptide TFA Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Compstatin control peptide TFA 的其他形式现货产品:

Compstatin control peptide

生物活性

Compstatin control peptide TFA is a complement inhibitor that binds and inhibits cleavage of complement C3[1].

分子量

1602.69

Formula

C68H102F3N23O19
Sequence Shortening

IAVVQDWGHHRAT-NH2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Ronald D Gorham Jr, et al. Novel compstatin family peptides inhibit complement activation by drusen-like deposits in human retinal pigmented epithelial cell cultures. Exp Eye Res. 2013 Nov;116:96-108.

Compstatin(Synonyms: 坎普他汀)

发表于由

Compstatin;(Synonyms: 坎普他汀) 纯度: 98.34%

Compstatin 是一个 13 残基环肽，是补体系统 C3 的有效抑制剂，具有物种特异性。Compstatin 结合到狒狒的 C3，并对狒狒血液中的蛋白水解裂解具有抗性 （类似人类）。Compstatin 只抑制灵长类补体系统的激活。Compstatin 对补体经典途径和旁路途径的 IC50 值分别为 63 μM 和 12 μM。

Compstatinamp;;(Synonyms: 坎普他汀)

Compstatin Chemical Structure

CAS No. : 206645-99-0

规格 价格 是否有货 数量
500 μg ￥650 In-stock
1 mg ￥900 In-stock
5 mg ￥2200 In-stock
10 mg ; 询价 ;
50 mg ; 询价 ;

* Please select Quantity before adding items.

Compstatin 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Compstatin, a 13-residue cyclic peptide, is a potent inhibitor of the complement system C3 with species specificity. Compstatin binds to baboon C3 and is resistant to proteolytic cleavage in baboon blood (similar to humans). Compstatin inhibits only the activation of primates’ complement system. Compstatin exhibits IC50 values of 63 μM and 12 μM for classical and alterative complement pathway, respectively[1][2][3][4].

体外研究
(In Vitro)

Compstatin exhibits an in vitro half-life in human blood of about2 hr[2].
In solution, compstatin forms a β-turn at residues Gln-5–Gly-8 with the disulfide bridge Cys-2–Cys12, residues Ile-1–Val-4, and Thr-13, forming a hydrophobic cluster[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究
(In Vivo)

Compstatin (21 mg/kg) produces complete inhibition when given as a combination of bolus injection and infusion. Compstatin completely inhibits in vivo heparin/protamine-induced complement activation without adverse effects on heart rate or systemic arterial, central venous, and pulmonary arterial pressures[1].
Compstatin is stable in baboon plasma for more than 24 h[1].
Pig xenografts survival is significantly longer in the Compstatin perfused group than in the control group[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Juvenile baboons (P. Anubis) weighing 10.5-28.8 kg[1].
Dosage: 50, 25 mg/kg 60 min after heparin and 2 min before protamine.
Administration: A bolus injection.
Result: Completely inhibited complement activation induced by heparin–protamine complexes.

分子量

1550.77

Formula

C66H99N23O17S2
CAS 号

206645-99-0
Sequence

Ile-Cys-Val-Val-Gln-Asp-Trp-Gly-His-His-Arg-Cys-Thr-NH2 (Disulfide bridge: Cys2-Cys12)
Sequence Shortening

ICVVQDWGHHRCT-NH2 (Disulfide bridge: Cys2-Cys12)
中文名称

坎普他汀
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Soulika AM, et al. Inhibition of heparin/protamine complex-induced complement activation by Compstatin in baboons. Clin Immunol. 2000 Sep;96(3):212-21.

    [2]. Fiane AE, et al. Compstatin, a peptide inhibitor of C3, prolongs survival of ex vivo perfused pig xenografts. Xenotransplantation. 1999 Feb;6(1):52-65.

    [3]. Bert J C Janssen, et al. Structure of compstatin in complex with complement component C3c reveals a new mechanism of complement inhibition. J Biol Chem. 2007 Oct 5;282(40):29241-7.

    [4]. A Sahu, et al. Inhibition of human complement by a C3-binding peptide isolated from a phage-displayed random peptide library. J Immunol. 1996 Jul 15;157(2):884-91.