Peptide C105Y TFA，基于 α1-抗胰蛋白酶 359-374 残基氨基酸序列合成的细胞穿透性肽，能增强 DNA 纳米粒的基因表达。
Peptide C105Y TFA Chemical Structure
规格
是否有货
100 mg
;
询价
;
250 mg
;
询价
;
500 mg
;
询价
;
* Please select Quantity before adding items.
生物活性
Peptide C105Y TFA, a synthetic and cell-penetrating peptide based on the amino acid sequence corresponding to residues 359-374 of α1-antitrypsin, enhances gene expression from DNA nanoparticles[1].
体外研究 (In Vitro)
When human hepatoma cells (HuH7) were incubated with fluorescently labeled C105Y for as little as 3 min, C105Y displays nuclear and cytoplasmic staining with enrichment of fluorescent signal in the nucleus and nucleolus. C105Y peptide is routed to the nucleolus very rapidly in an energy-dependent fashion, whereas membrane translocation and nuclear localization are energy-independent. When tested the involvement of known endocytosis pathways in uptake and trafficking of this peptide, C105Y peptide is internalized by a clathrin- and caveolin-independent pathway, although lipid raft-mediated endocytosis may play a role in peptide intracellular trafficking[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
2108.42
Formula
C99H149F3N20O25S
Sequence Shortening
CSIPPEVKFNKPFVYLI
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献
[1]. Rhee M, et al. Mechanism of uptake of C105Y, a novel cell-penetrating peptide. J Biol Chem. 2006;281(2):1233-1240.
Cy3 NHS ester (Cyanine3 NHS ester) is a yellow emitting fluorescent dye for labeling amino-groups in biomolecules. Used to label soluble proteins, peptides, and oligonucleotides/DNA.
分子量
590.15
Formula
C34H40ClN3O4
CAS 号
1393363-07-9
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
-20deg;C, protect from light, stored under argon
*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light, stored under argon)
溶解性数据
In Vitro:;
DMSO : 250 mg/mL (423.62 mM; Need ultrasonic)
配制储备液
浓度溶剂体积质量
1 mg
5 mg
10 mg
1 mM
1.6945 mL
8.4724 mL
16.9448 mL
5 mM
0.3389 mL
1.6945 mL
3.3890 mL
10 mM
0.1694 mL
0.8472 mL
1.6945 mL
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light, stored under argon)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
Cyanine 3 Tyramide (Tyramide-Cy3), an orange fluorescent dye, is utilized as reporter fluorescent substrate for horseradish peroxidase (HRP)-catalyzed deposition that is signal amplification technique in immunoassay and in situ hybridization of nucleic acids[1][2].
分子量
749.94
Formula
C39H47N3O8S2
CAS 号
174961-75-2
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献
[1]. Chao J, et, al. Immunofluorescence signal amplification by the enzyme-catalyzed deposition of a fluorescent reporter substrate (CARD). Cytometry. 1996 Jan 1;23(1):48-53.
[2]. Schmidt BF, et, al. Signal amplification in the detection of single-copy DNA and RNA by enzyme-catalyzed deposition (CARD) of the novel fluorescent reporter substrate Cy3.29-tyramide. J Histochem Cytochem. 1997 Mar;45(3):365-73.
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献
[1]. Finke A, et al. Designer Extracellular Matrix Based on DNA-Peptide Networks Generated by Polymerase ChainReaction. Angew Chem Int Ed Engl. 2016 Aug 16;55(34):10136-40.
Cy3 NHS ester (Cyanine3 NHS ester) is a yellow emitting fluorescent dye for labeling amino-groups in biomolecules. Used to label soluble proteins, peptides, and oligonucleotides/DNA.
分子量
590.15
Formula
C34H40ClN3O4
CAS 号
1393363-07-9
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
-20deg;C, protect from light, stored under argon
*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light, stored under argon)
溶解性数据
In Vitro:;
DMSO : 250 mg/mL (423.62 mM; Need ultrasonic)
配制储备液
浓度溶剂体积质量
1 mg
5 mg
10 mg
1 mM
1.6945 mL
8.4724 mL
16.9448 mL
5 mM
0.3389 mL
1.6945 mL
3.3890 mL
10 mM
0.1694 mL
0.8472 mL
1.6945 mL
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light, stored under argon)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献
[1]. Finke A, et al. Designer Extracellular Matrix Based on DNA-Peptide Networks Generated by Polymerase ChainReaction. Angew Chem Int Ed Engl. 2016 Aug 16;55(34):10136-40.
Cy5-SE (Cy5 NHS Ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reaction. This reagent is ideal for very cost-efficient labeling of soluble proteins, as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling. Excitation (nm):649, Emission (nm): 670.
体外研究 (In Vitro)
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Conjugation of SELP Analogues with Cy5-SE. 1. 1 mL of each 100 μg/mL SELP solution in 100 mM sodium bicarbonate buffer (pH 8.3) is mixed with 10 μL of 1.2 mg/mL Cy5 mono NHS-ester in 10% DMSO and incubates for 2 hours on ice. 2. To quench the reaction, 50 μL of 1 M Tris-HCl (pH 8.0) is added to the reaction solution. 3. Reaction mixtures are loaded onto 1.5 mL Sephadex G-25 columns, and Cy5-conjugated SELPs are eluted by centrifugation for 3 minutes at 1050g[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
753.88
Formula
C37H43N3O10S2
CAS 号
146368-14-1
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
-20deg;C, sealed storage, away from moisture and light
*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:;
DMSO : 25 mg/mL (33.16 mM; Need ultrasonic)
配制储备液
浓度溶剂体积质量
1 mg
5 mg
10 mg
1 mM
1.3265 mL
6.6324 mL
13.2647 mL
5 mM
0.2653 mL
1.3265 mL
2.6529 mL
10 mM
0.1326 mL
0.6632 mL
1.3265 mL
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献
[1]. Jeon HY, et al. Array-Based High-Throughput Analysis of Silk-Elastinlike Protein Polymer Degradation and C-Peptide Release by Proteases. Anal Chem. 2016;88(10):5398-5405.
Cy5-DBCO (DBCO-Sulfo-Cy5) is a near-infrared (NIR) red fluorescent dye with λabs and λem of 646 nm and 670 nm, respectively. Cy5-DBCO (DBCO-Sulfo-Cy5) is not suitable for staining intracellular components of permeabilezed cell, it may exhibits a high background.
IC50 Target
Red fluorescent dye
体外研究 (In Vitro)
Cy5-DBCO (DBCO-Sulfo-Cy5) reacts with azides via a copper-free “click chemistry” reaction and forms a stable triazole and does not need Cu-catalyst or elevated temperatures.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
1009.22
Formula
C52H56N4O11S3
CAS 号
1564286-24-3
运输条件
Room temperature in continental US; may vary elsewhere.
