Peptide C105Y

Peptide C105Y;

Peptide C105Y,基于 α1-抗胰蛋白酶 359-374 残基氨基酸序列合成的细胞穿透性肽,能增强 DNA 纳米粒的基因表达。

Peptide C105Yamp;;

Peptide C105Y Chemical Structure

CAS No. : 247572-63-0

规格 价格 是否有货
5 mg ¥4000 询问价格 货期
10 mg ¥6800 询问价格 货期

* Please select Quantity before adding items.

生物活性

Peptide C105Y, a synthetic and cell-penetrating peptide based on the amino acid sequence corresponding to residues 359-374 of α1-antitrypsin, enhances gene expression from DNA nanoparticles[1].

体外研究
(In Vitro)

When human hepatoma cells (HuH7) were incubated with fluorescently labeled C105Y for as little as 3 min, C105Y displays nuclear and cytoplasmic staining with enrichment of fluorescent signal in the nucleus and nucleolus. C105Y peptide is routed to the nucleolus very rapidly in an energy-dependent fashion, whereas membrane translocation and nuclear localization are energy-independent. When tested the involvement of known endocytosis pathways in uptake and trafficking of this peptide, C105Y peptide is internalized by a clathrin- and caveolin-independent pathway, although lipid raft-mediated endocytosis may play a role in peptide intracellular trafficking[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1994.40

Formula

C97H148N20O23S

CAS 号

247572-63-0

Sequence

Cys-Ser-Ile-Pro-Pro-Glu-Val-Lys-Phe-Asn-Lys-Pro-Phe-Val-Tyr-Leu-Ile

Sequence Shortening

CSIPPEVKFNKPFVYLI

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Rhee M, et al. Mechanism of uptake of C105Y, a novel cell-penetrating peptide. J Biol Chem. 2006 Jan 13;281(2):1233-40.

Peptide C105Y TFA

Peptide C105Y TFA;

Peptide C105Y TFA,基于 α1-抗胰蛋白酶 359-374 残基氨基酸序列合成的细胞穿透性肽,能增强 DNA 纳米粒的基因表达。

Peptide C105Y TFAamp;;

Peptide C105Y TFA Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

Peptide C105Y TFA, a synthetic and cell-penetrating peptide based on the amino acid sequence corresponding to residues 359-374 of α1-antitrypsin, enhances gene expression from DNA nanoparticles[1].

体外研究
(In Vitro)

When human hepatoma cells (HuH7) were incubated with fluorescently labeled C105Y for as little as 3 min, C105Y displays nuclear and cytoplasmic staining with enrichment of fluorescent signal in the nucleus and nucleolus. C105Y peptide is routed to the nucleolus very rapidly in an energy-dependent fashion, whereas membrane translocation and nuclear localization are energy-independent. When tested the involvement of known endocytosis pathways in uptake and trafficking of this peptide, C105Y peptide is internalized by a clathrin- and caveolin-independent pathway, although lipid raft-mediated endocytosis may play a role in peptide intracellular trafficking[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

2108.42

Formula

C99H149F3N20O25S

Sequence Shortening

CSIPPEVKFNKPFVYLI

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Rhee M, et al. Mechanism of uptake of C105Y, a novel cell-penetrating peptide. J Biol Chem. 2006;281(2):1233-1240.

荧光染料Cy5.5-SE(Synonyms: Cyanine5.5 NHS ester)

荧光染料Dye Reagents Cy5.5-SE;(Synonyms: Cyanine5.5 NHS ester) 纯度: 96.69%

Cy5.5-SE (Cyanine5.5 NHS ester) 是一种花青染料,可标记多肽、蛋白和寡核苷酸中的氨基。

Cy5.5-SEamp;;(Synonyms: Cyanine5.5 NHS ester)

Cy5.5-SE Chemical Structure

CAS No. : 442912-55-2

规格 价格 是否有货 数量
5 mg ¥6500 In-stock
10 mg ¥10500 询价
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

Cy5.5-SE (Cyanine5.5 NHS ester) is a cyanine dye, labeling amino-groups in peptides, proteins, and oligonucleotides.

分子量

1014.13

Formula

C45H47N3O16S4

CAS 号

442912-55-2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)

荧光染料Cy5.5-SE(Synonyms: Cyanine5.5 NHS ester)

荧光染料Dye Reagents Cy5.5-SE;(Synonyms: Cyanine5.5 NHS ester) 纯度: 96.69%

Cy5.5-SE (Cyanine5.5 NHS ester) 是一种花青染料,可标记多肽、蛋白和寡核苷酸中的氨基。

Cy5.5-SE(Synonyms: Cyanine5.5 NHS ester)

Cy5.5-SE Chemical Structure

CAS No. : 442912-55-2

规格 价格 是否有货 数量
5 mg ¥6500 In-stock
10 mg ¥10500 询价
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

Cy5.5-SE (Cyanine5.5 NHS ester) is a cyanine dye, labeling amino-groups in peptides, proteins, and oligonucleotides.

分子量

1014.13

Formula

C45H47N3O16S4

CAS 号

442912-55-2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)

荧光染料Cy3 NHS ester(Synonyms: Cyanine3 NHS ester)

荧光染料Dye Reagents Cy3 NHS ester;(Synonyms: Cyanine3 NHS ester) 纯度: 99.05%

Cy3 NHS ester (Cyanine3 NHS ester) 是一种黄色荧光染料,用于标记生物分子中的氨基。常用于标记可溶性蛋白质、多肽和寡核苷酸/DNA。

Cy3 NHS esteramp;;(Synonyms: Cyanine3 NHS ester)

Cy3 NHS ester Chemical Structure

CAS No. : 1393363-07-9

规格 价格 是否有货 数量
10 mg ¥2800 In-stock
25 mg ¥4000 In-stock
50 mg ¥6900 In-stock
100 mg ; 询价 ;
200 mg ; 询价 ;

* Please select Quantity before adding items.

Cy3 NHS ester 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Cy3 NHS ester (Cyanine3 NHS ester) is a yellow emitting fluorescent dye for labeling amino-groups in biomolecules. Used to label soluble proteins, peptides, and oligonucleotides/DNA.

分子量

590.15

Formula

C34H40ClN3O4

CAS 号

1393363-07-9

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, protect from light, stored under argon

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light, stored under argon)

溶解性数据
In Vitro:;

DMSO : 250 mg/mL (423.62 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.6945 mL 8.4724 mL 16.9448 mL
5 mM 0.3389 mL 1.6945 mL 3.3890 mL
10 mM 0.1694 mL 0.8472 mL 1.6945 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light, stored under argon)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.08 mg/mL (3.52 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (3.52 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (3.52 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (3.52 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
*以上所有助溶剂都可在 MCE 网站选购。

荧光染料Cyanine 3 Tyramide(Synonyms: Tyramide-Cy3)

荧光染料Dye Reagents Cyanine 3 Tyramide;(Synonyms: Tyramide-Cy3)

Cyanine 3 Tyramide (Tyramide-Cy3) 是一种橙色荧光染料,被用作辣根过氧化物酶 HRP 催化沉积的报告荧光底物,是一种免疫测定和核酸原位杂交中的信号放大技术。

Cyanine 3 Tyramide(Synonyms: Tyramide-Cy3)

Cyanine 3 Tyramide Chemical Structure

CAS No. : 174961-75-2

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

Cyanine 3 Tyramide (Tyramide-Cy3), an orange fluorescent dye, is utilized as reporter fluorescent substrate for horseradish peroxidase (HRP)-catalyzed deposition that is signal amplification technique in immunoassay and in situ hybridization of nucleic acids[1][2].

分子量

749.94

Formula

C39H47N3O8S2

CAS 号

174961-75-2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Chao J, et, al. Immunofluorescence signal amplification by the enzyme-catalyzed deposition of a fluorescent reporter substrate (CARD). Cytometry. 1996 Jan 1;23(1):48-53.

    [2]. Schmidt BF, et, al. Signal amplification in the detection of single-copy DNA and RNA by enzyme-catalyzed deposition (CARD) of the novel fluorescent reporter substrate Cy3.29-tyramide. J Histochem Cytochem. 1997 Mar;45(3):365-73.

荧光染料CY5-N3(Synonyms: Sulfo-Cyanine5-azide)

荧光染料Dye Reagents CY5-N3;(Synonyms: Sulfo-Cyanine5-azide) 纯度: 98.40%

CY5-N3 是一种 Cy5-叠氮化物,是一种荧光染料。

CY5-N3amp;;(Synonyms: Sulfo-Cyanine5-azide)

CY5-N3 Chemical Structure

CAS No. : 1621101-43-6

规格 价格 是否有货 数量
5 mg ¥3500 In-stock
10 mg ; 询价 ;
50 mg ; 询价 ;

* Please select Quantity before adding items.

CY5-N3 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

CY5-N3 is a Cy5-azide, which is a fluorescent dye.

体外研究
(In Vitro)

CY5-N3 can be used to monitor the CuAAC of branched DNA with Cy5-azide by HPLC[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

738.92

Formula

C36H46N6O7S2

CAS 号

1621101-43-6

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, protect from light

*该产品在溶液状态不稳定,建议您现用现配,即刻使用。

溶解性数据
In Vitro:;

DMSO : 25 mg/mL (33.83 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.3533 mL 6.7666 mL 13.5333 mL
5 mM 0.2707 mL 1.3533 mL 2.7067 mL
10 mM 0.1353 mL 0.6767 mL 1.3533 mL

*

请根据产品在不同溶剂中的溶解度,选择合适的溶剂配制储备液;该产品在溶液状态不稳定,建议您现用现配,即刻使用

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (3.38 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.38 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (3.38 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.38 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Finke A, et al. Designer Extracellular Matrix Based on DNA-Peptide Networks Generated by Polymerase ChainReaction. Angew Chem Int Ed Engl. 2016 Aug 16;55(34):10136-40.

荧光染料Cy3 NHS ester(Synonyms: Cyanine3 NHS ester)

荧光染料Dye Reagents Cy3 NHS ester;(Synonyms: Cyanine3 NHS ester) 纯度: 99.05%

Cy3 NHS ester (Cyanine3 NHS ester) 是一种黄色荧光染料,用于标记生物分子中的氨基。常用于标记可溶性蛋白质、多肽和寡核苷酸/DNA。

Cy3 NHS ester(Synonyms: Cyanine3 NHS ester)

Cy3 NHS ester Chemical Structure

CAS No. : 1393363-07-9

规格 价格 是否有货 数量
10 mg ¥2800 In-stock
25 mg ¥4000 In-stock
50 mg ¥6900 In-stock
100 mg ; 询价 ;
200 mg ; 询价 ;

* Please select Quantity before adding items.

Cy3 NHS ester 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Cy3 NHS ester (Cyanine3 NHS ester) is a yellow emitting fluorescent dye for labeling amino-groups in biomolecules. Used to label soluble proteins, peptides, and oligonucleotides/DNA.

分子量

590.15

Formula

C34H40ClN3O4

CAS 号

1393363-07-9

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, protect from light, stored under argon

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light, stored under argon)

溶解性数据
In Vitro:;

DMSO : 250 mg/mL (423.62 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.6945 mL 8.4724 mL 16.9448 mL
5 mM 0.3389 mL 1.6945 mL 3.3890 mL
10 mM 0.1694 mL 0.8472 mL 1.6945 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light, stored under argon)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.08 mg/mL (3.52 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (3.52 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (3.52 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (3.52 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
*以上所有助溶剂都可在 MCE 网站选购。

荧光染料CY5-N3(Synonyms: Sulfo-Cyanine5-azide)

荧光染料Dye Reagents CY5-N3;(Synonyms: Sulfo-Cyanine5-azide) 纯度: 98.40%

CY5-N3 是一种 Cy5-叠氮化物,是一种荧光染料。

CY5-N3(Synonyms: Sulfo-Cyanine5-azide)

CY5-N3 Chemical Structure

CAS No. : 1621101-43-6

规格 价格 是否有货 数量
5 mg ¥3500 In-stock
10 mg ; 询价 ;
50 mg ; 询价 ;

* Please select Quantity before adding items.

CY5-N3 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

CY5-N3 is a Cy5-azide, which is a fluorescent dye.

体外研究
(In Vitro)

CY5-N3 can be used to monitor the CuAAC of branched DNA with Cy5-azide by HPLC[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

738.92

Formula

C36H46N6O7S2

CAS 号

1621101-43-6

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, protect from light

*该产品在溶液状态不稳定,建议您现用现配,即刻使用。

溶解性数据
In Vitro:;

DMSO : 25 mg/mL (33.83 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.3533 mL 6.7666 mL 13.5333 mL
5 mM 0.2707 mL 1.3533 mL 2.7067 mL
10 mM 0.1353 mL 0.6767 mL 1.3533 mL

*

请根据产品在不同溶剂中的溶解度,选择合适的溶剂配制储备液;该产品在溶液状态不稳定,建议您现用现配,即刻使用

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (3.38 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.38 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (3.38 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.38 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Finke A, et al. Designer Extracellular Matrix Based on DNA-Peptide Networks Generated by Polymerase ChainReaction. Angew Chem Int Ed Engl. 2016 Aug 16;55(34):10136-40.

CY5-SE(Synonyms: Cy5 NHS Ester Sulfo-Cyanine5 Succinimidyl Ester)

CY5-SE;(Synonyms: Cy5 NHS Ester; Sulfo-Cyanine5 Succinimidyl Ester) 纯度: ge;98.0%

Cy5-SE (Cy5 NHS Ester) 是一种用于标记肽, 蛋白质, 寡核苷酸的氨基基团的反应染料。 激发波长 (nm): 649, 发射波长 (nm): 670。

CY5-SEamp;;(Synonyms: Cy5 NHS Ester;  Sulfo-Cyanine5 Succinimidyl Ester)

CY5-SE Chemical Structure

CAS No. : 146368-14-1

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ¥1824 In-stock
5 mg ¥1500 In-stock
10 mg ¥2200 In-stock
25 mg ¥4400 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

CY5-SE 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Cy5-SE (Cy5 NHS Ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reaction. This reagent is ideal for very cost-efficient labeling of soluble proteins, as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling. Excitation (nm):649, Emission (nm): 670.

体外研究
(In Vitro)

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Conjugation of SELP Analogues with Cy5-SE.
1. 1 mL of each 100 μg/mL SELP solution in 100 mM sodium bicarbonate buffer (pH 8.3) is mixed with 10 μL of 1.2 mg/mL Cy5 mono NHS-ester in 10% DMSO and incubates for 2 hours on ice.
2. To quench the reaction, 50 μL of 1 M Tris-HCl (pH 8.0) is added to the reaction solution.
3. Reaction mixtures are loaded onto 1.5 mL Sephadex G-25 columns, and Cy5-conjugated SELPs are eluted by centrifugation for 3 minutes at 1050g[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

753.88

Formula

C37H43N3O10S2

CAS 号

146368-14-1

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)

溶解性数据
In Vitro:;

DMSO : 25 mg/mL (33.16 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.3265 mL 6.6324 mL 13.2647 mL
5 mM 0.2653 mL 1.3265 mL 2.6529 mL
10 mM 0.1326 mL 0.6632 mL 1.3265 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (3.32 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.32 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (3.32 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.32 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Jeon HY, et al. Array-Based High-Throughput Analysis of Silk-Elastinlike Protein Polymer Degradation and C-Peptide Release by Proteases. Anal Chem. 2016;88(10):5398-5405.