GRGDSPC

GRGDSPC;

GRGDSPC 是一种含 7 个氨基酸的多肽,是一种末端巯基化的细胞粘附肽。

GRGDSPCamp;;

GRGDSPC Chemical Structure

CAS No. : 91575-26-7

规格 价格 是否有货
1 mg ¥700 询问价格 货期
5 mg ¥2100 询问价格 货期
10 mg ¥3100 询问价格 货期

* Please select Quantity before adding items.

生物活性

GRGDSPC, a 7-amino acid peptide, is a thiolated cell adhesion peptide[1].

体外研究
(In Vitro)

GRGDSPC is conjugated to acrylated dextran via thiol-acrylate reaction to regulate the interactions of human mesenchymal stem cells (hMSCs) with the photocrosslinkable hydrogels. To determine the conjugation kinetics and efficiency of GRGDSPC peptide to DEX-MAES16, various GRGDSPC concentrations (i.e., 5, 10 and 20 mg/1 g DEX-MAES16) are conjugated to the acrylated Dextran (DEX) macromer over time (0.25, 0.5, 1 and 3h) in PBS at pH 7.8 and the free thiol groups of unreacted peptides are quantified using Ellman’s assay. In addition, the reaction kinetics of the thiol-peptide to acrylated (DEX-MAES16) and methacrylated (DEX-HEMA16) macromers are compared. As early as 15 min conjugation, with 5, 10 and 20 mg of GRGDSPC peptide/1 g modified DEX, the peptide conjugation efficiencies with DEX-MAES are 105.40, 94.10 and 87.45%, respectively, while for the reaction with the DEX-HEMA they are 0.73, 15.78 and 18.42%, respectively. After 1h, the GRGDSPC conjugation with DEX-MAES is completed with the peptide concentration of 10 mg, but only 35.66% of the thiol groups of the peptide react with DEX-HEMA. The reaction kinetics are also monitored at 3 h of conjugation, and all of the 20 mg GRGDSPC peptide reacts with acrylated DEX compared to only 32.53% for the methacrylated DEX at this time point[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

690.73

Formula

C25H42N10O11S

CAS 号

91575-26-7

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献
  • [1]. Nguyen MK, et al. Photocrosslinkable, biodegradable hydrogels with controlled cell adhesivity for prolonged siRNAdelivery to hMSCs to enhance their osteogenic differentiation. J Mater Chem B. 2017 Jan 21;5(3):485-495.

Endomorphin 2(Synonyms: 内吗啡肽 2)

Endomorphin 2;(Synonyms: 内吗啡肽 2)

Endomorphin 2,一种高度选择性的高亲和力μ-opioid受体激动剂 , 对kappa3结合位点具有高亲和力 ,Ki为 20 到 30 nM 之间。

Endomorphin 2amp;;(Synonyms: 内吗啡肽 2)

Endomorphin 2 Chemical Structure

CAS No. : 141801-26-5

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Endomorphin 2 的其他形式现货产品:

Endomorphin 2 TFA

生物活性

Endomorphin 2, a high affinity, highly selective agonist of the μ-opioid receptor, displays reasonable affinities for kappa3 binding sites, with Ki value between 20 and 30 nM.

IC50 Target

Ki: 20-30 nM (kappa3 opioid receptor)[1]

体外研究
(In Vitro)

Endomorphin 2 is an endogenous opioid peptide and one of the two Endomorphins. It is a high affinity, highly selective agonist of the μ-opioid receptor, and along with Endomorphin 1 (EM-2). The two Endomorphins display reasonable affinities for kappa3 binding sites, with Ki values between 20 and 30 nM. Endomorphin 1 and Endomorphin 2 compete both μ1 and μ2 receptor sites quite potently. Endomorphins have little appreciable affinity for either delta or kappa1 binding sites, with Ki values greater than 500 nM[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Both Endomorphin 1 and Endomorphin 2 are potent analgesics with peak effects seen at 10 and 15 min, respectively. All subsequent studies are performed at peak effect. Both compounds are fully active supraspinally and spinally, with no indication of ceiling effects. Endomorphin 1 is significantly more potent spinally than supraspinally and, at the spinal level, it is significantly more potent than Endomorphin 2. The response of both agents are readily reversed by naloxone. β-FNA, a highly selective μ antagonist, effectively reverses the actions of both Endomorphins. Both Endomorphin 1 and Endomorphin 2 display a profile similar to morphine. Neither compound have analgesic activity in CXBK mice at a dose which produced over 70% analgesia in control CD-1 mice[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

571.67

Formula

C32H37N5O5

CAS 号

141801-26-5

Sequence

Tyr-Pro-Phe-Phe-NH2

Sequence Shortening

YPFF-NH2

中文名称

内吗啡肽 2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献
  • [1]. Goldberg IE, et al. Pharmacological characterization of endomorphin-1 and endomorphin-2 in mouse brain. J Pharmacol Exp Ther. 1998 Aug;286(2):1007-13.

Kinase Assay
[1]

125I-Endomorphin 1 or 125I-Endomorphin 2 binding (0.2 nM) is performed in potassium phosphate buffer (50 mM, pH 7.4; 0.5 mL) with MgCl2 (5 mM) at a tissue concentration of 10 mg wet weight/mL for brains or 0.06 mg protein/mL for MOR-1/CHO cells. Specific binding is determined in the presence and absence of either 1 μM of the corresponding unlabeled peptide. The entire mixture is then incubated at 25°C for 1 hr and filtered over no. 32 glass fiber filters which have been presoaked for 1 hr in 0.5% polyethylenimine and washed twice with ice cold Tris buffer using a Brandel cell harvester. The filters are then counted on a Packard Cobra gamma counter. The other opioid receptor binding assays are performed[1] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

Mice[1]
Groups of mice are treated i.c.v. with Endomorphin 1 (12 μg) or Endomorphin 2 (3 μg) 15 min before a 0.5-cc charcoal meal (2.5% gum tragacanth,10% activated charcoal in water). The mice are killed 30 min later and the distance the charcoal traveled is measured.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Goldberg IE, et al. Pharmacological characterization of endomorphin-1 and endomorphin-2 in mouse brain. J Pharmacol Exp Ther. 1998 Aug;286(2):1007-13.

日本同仁化学DiBAC4(3)试剂货号:D545  C27H39N4NaO6| DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

DiBAC4(3)试剂货号:D545
Bis(1,3-dibutylbarbituric acid)trimethine oxonol, sodium salt
DiBAC4(3)
商品信息
储存条件:0-5度保存
运输条件:室温
分子式:

 C27H39N4NaO6

分子量:

538.61

 

特点:

 

● 可用于流式细胞仪、共聚焦显微镜

● 操作简便

 

SDS下载

选择规格:
25mg

现货 

 
荧光探针

性质
参考文献
规格性状

性质

它是Bis-oxonol型阴离子膜电位敏感染料,随着细胞膜去极化,其在细胞质中的分布增加,荧光增强。 据报道,在BICR / M1R-k细胞(大鼠乳腺癌细胞)中,在-25至-90mV的膜电位范围内获得了约1%/ mV的荧光强度变化。 与电势变化相关的响应遵循一阶速率方程,其速率常数为0.1至0.8 / min。

由于可以被Ar激光(488nm)激发,因此可以用于流式细胞术,共聚焦显微镜等。 除膜电位测量外,还有在细胞活力和离子通道活性评估中应用的实例。 它也可以用于高通量筛选(HTS)细胞反应。

*有关使用方法,请参照操作说明书。

参考文献

1) D. E. Epps, M. L. Wolfe and V. Groppi, “Characterization of the Steady-state and Dynamic Fluorescence Properties of the Potential-sensitive Dye Bis-(1,3-dibutylbarbituric acid)trimethine oxonol(Dibac4(3)) in Model Systems and Cells”, Chem. Phys. Lipids199469, 137.
2) T. Braner, D. F. Hulser and R. J. Strasser, “Comparative Measurements of Membrane Potentials with Microelectrodes and Voltage-sensitibe Dyes”, Biochim. Biophys. Acta1984, 771, 208.
3) T. T. Rohn, A. Sauvadet, C. Pavoine and F. Pecker, “Xanthine Affects [Ca2+]i and Contractile Responses of Ventricular Cardiocytes to Electrical Stimulation”, Am. J. Physiol.1997273, C909.
4) D. J. Mason, R. Allman, J. M. Stark and D. Lloyd, “Rapid Estimation of Bacterial Antibiotic Susceptibility with Flow Cytometry”, J. Microsc., 1994176, 8.
5) U. Langheinrich and J. Daut, “Hyperpolarization of Isolated Capillaries from Guinea-pig Heart Induced by K+ Channel Openers and Glucose Deprivation”, J. Physiol., 1997502, 397.
6) V. Dall’Asta, R. Getti, G. Orlandini, P. A. Rossi, B. M. Rotoli, R. Sala, O. Bussolati and G. C. Gazzola, “Membrane Potential Changes Visualized in Complete Growth Media through Confocal Laser Scanning Microscopy of bis-Oxonol-loaded Cells”, Exp. Cell Res., 1997231, 260.
7) K. S. Schroeder and B. D. Neagle, “FLIPR: A New Instrument for Accurate, High Throughput Optical Screening”, J. Biomol. Screening19961, 75.

规格性状

规格性状:

本品为橙红色至红色粉末。

纯度(HPLC):98.0%或更高

二甲基亚砜可溶:测试合格性

摩尔消光系数:140,000或更高(约493 nm)

红外光谱:测试合格

处理条件

1.保存方法:冷藏

日本同仁化学DAB试剂货号:D006 3,3′-Diaminobenzidine, tetrahydrochloride CAS号:7411-49-6| DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

DAB试剂货号:D006
3,3′-Diaminobenzidine, tetrahydrochloride
CAS号:7411-49-6
商品信息
储存条件:0-5度保存,避光
运输条件:室温
分子式:

C12H18Cl4N4

分子量:

360.11

SDS下载

选择规格:
1g

期货

 
细胞染色

DAB试剂货号:D006 3,3′-Diaminobenzidine, tetrahydrochloride CAS号:7411-49-6

DAB试剂货号:D006 3,3′-Diaminobenzidine, tetrahydrochloride CAS号:7411-49-6

产品简介
染色溶液的制备
参考文献
操作和储存条件

产品简介

DAB试剂货号:D006 3,3′-Diaminobenzidine, tetrahydrochloride CAS号:7411-49-6

DAB是在免疫组化中最常用的氧化型过氧化物酶显色底物之一。在过氧化氢和过氧化酶存在时,DAB被氧化成棕色的显色底物。这种棕色的显色底物是苯胺黑类化合物,可以牢固的沉积在细胞膜或组织上的过氧化物酶的周围。市面上销售的DAB往往由于生产和保存期间的氧化问题呈褐色。而同仁化学研究所的提供的高纯度DAB的性状呈白色或略带红色的灰色粉末,可以得到灵敏度更高的染色效果。

染色溶液的制备

1. 用1 ml PBS溶解9 mg DAB制备成100 x DAB solution。

2. 用1 ml PBS混合5 μl 的30%过氧化氢酶溶液制备成200 x H2O2 solution。

3. 向1 ml PBS中加入10 μl 的100 x DAB solution和5 μl的 200 x 的H2O2 solution制成Staining soluiton1)

4. 将Staining solution加入到染色容器中并将样品在室温下培养1 h2)

5. 用PBS清洗样品数次使染色反应停止。

1) Staining solution不稳定,请现配现用。

2) 为了获得更好的染色效果,可以在加入Staining solution之前预先用0.09 mg DAB/PBS溶液培养样品10 min。

参考文献

1) A. B. Novikoff, et al., Studies on Microperoxisomes V. Are Microperoxisomes Ubiquitous in Mammalian Cells. J Histochem Cytochem1973;21:737.

2) V. Herzog, et al., A New Sensitive Colorimetric Assay for Peroxidaase Using 3, 3 EDiaminobenzidine as Hydrogen Donor. Anal Biochem1973;55:554.

3) H. Yamada, et al., Improvement of Technique of Immunohistochemical Demonstraction of Bioactive Substances in the Central Nervous System. Acta Histochem Cytochem1987;20:629.

4) K. L. Cheng, Determination of Traces of Selenium 3,3 EDiaminobenzidine as Selenium(IV) Organic Reagent. Anal Chem1956;28:1738.

操作和储存条件

规格:

性状:红色或略带红色的灰色粉末

纯度(滴定法):≥97.0%(干燥固态)

水中溶解度:通过检测

Tris缓冲液中溶解度:通过检测

吸光度(硒化合物):≧ 0.500 (420 nm附近)

干燥失重:≤5.0%

硫酸盐灰分:≤0.05%

IR谱图:一致

操作和储存条件:

0-5℃,避光

危险/有害性标志

关联产品

DAB试剂货号:D006 3,3′-Diaminobenzidine, tetrahydrochloride CAS号:7411-49-6
Calcein-AM/PI细胞双染试剂盒
活死细胞双染试剂盒

DAB试剂货号:D006 3,3′-Diaminobenzidine, tetrahydrochloride CAS号:7411-49-6
活细胞质染色-绿色——Calcein-AM
3′,6′-Di(O-acetyl)-4′,5′-bis[N,N-bis(carboxymethyl)aminomethyl]fluorescein, tetraacetoxymethyl ester

DAB试剂货号:D006 3,3′-Diaminobenzidine, tetrahydrochloride CAS号:7411-49-6
Cellstain- Hoechst 33342 solution试剂
2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole, trihydrochloride

DAB试剂货号:D006 3,3′-Diaminobenzidine, tetrahydrochloride CAS号:7411-49-6
PlasMem Bright Green细胞膜染色试剂
细胞膜染色试剂—绿色

DAB试剂货号:D006 3,3′-Diaminobenzidine, tetrahydrochloride CAS号:7411-49-6
Cellstain- CFSE试剂
5- or 6-(N-Succinimidyloxycarbonyl)fluorescein 3′,6′-diacetate

在线小工具

实验工具|稀释计算器|摩尔浓度计算器

日本同仁化学Cellstain- DAPI solution试剂货号:D523 4’6-二脒-2-苯胺,二盐酸盐 水溶液 Cellstain- DAPI solution| DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

Cellstain- DAPI solution试剂货号:D523
4’6-二脒-2-苯胺,二盐酸盐 水溶液
Cellstain- DAPI solution
商品信息
储存条件:0-5度保存,避光
运输条件:室温
分子式:

C16H17Cl2N5

分子量:

350.25

SDS下载

选择规格:
1ml

现货

 
细胞染色

关联产品

Cellstain- DAPI solution试剂货号:D523 4’6-二脒-2-苯胺,二盐酸盐 水溶液 Cellstain- DAPI solution
Calcein-AM/PI细胞双染试剂盒
活死细胞双染试剂盒

Cellstain- DAPI solution试剂货号:D523 4’6-二脒-2-苯胺,二盐酸盐 水溶液 Cellstain- DAPI solution
活细胞质染色-绿色——Calcein-AM
3′,6′-Di(O-acetyl)-4′,5′-bis[N,N-bis(carboxymethyl)aminomethyl]fluorescein, tetraacetoxymethyl ester

Cellstain- DAPI solution试剂货号:D523 4’6-二脒-2-苯胺,二盐酸盐 水溶液 Cellstain- DAPI solution
Cellstain- Hoechst 33342 solution试剂
2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole, trihydrochloride

Cellstain- DAPI solution试剂货号:D523 4’6-二脒-2-苯胺,二盐酸盐 水溶液 Cellstain- DAPI solution
PlasMem Bright Green细胞膜染色试剂
细胞膜染色试剂—绿色

Cellstain- DAPI solution试剂货号:D523 4’6-二脒-2-苯胺,二盐酸盐 水溶液 Cellstain- DAPI solution
Cellstain- CFSE试剂
5- or 6-(N-Succinimidyloxycarbonyl)fluorescein 3′,6′-diacetate

在线小工具

实验工具|稀释计算器|摩尔浓度计算器

日本同仁化学Cellstain- DAPI试剂货号:D212 4’6-二脒-2-苯胺,二盐酸盐 Cellstain- DAPI| DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

Cellstain- DAPI试剂货号:D212
4’6-二脒-2-苯胺,二盐酸盐
Cellstain- DAPI
商品信息
储存条件:-20度保存,避光
运输条件:室温
分子式:

C16H17Cl2N5

分子量:

350.25

特点:

 

● 可用于流式细胞仪

● 活细胞核染色试剂

SDS下载

选择规格:
1 mg

现货

细胞染色

Cellstain- DAPI试剂货号:D212 4’6-二脒-2-苯胺,二盐酸盐 Cellstain- DAPI

Cellstain- DAPI试剂货号:D212 4’6-二脒-2-苯胺,二盐酸盐 Cellstain- DAPI

规格性状
产品概述
荧光特性
操作说明
文献
常见问题Q&A

规格性状

规格

 

特性:该产物为黄色至微黄色的绿棕色粉末或固体,可溶于水和稀盐酸。

水溶性:试验成功

NMR光谱:试验成功

产品概述

荧光染料DAPI是一种可对DNA 染色的细胞核染色试剂,常用于细胞凋亡检测。DAPI是含有特定AT序列DNA的一种嵌入剂,它能像Hoechst染料一样粘附在DNA双螺旋的小沟区。尽管DAPI不能通过活细胞膜,但却能穿透扰乱的细胞膜而对细胞核染色。DAPI具有很高的光漂白承受水平,能用来检测酵母线粒体DNA,叶绿体DNA,病毒DNA,microplasm  DNA以及染色体DNA。DAPI-DNA复合物的激发和发射波长分别为360 nm和460 nm。

Cellstain- DAPI试剂货号:D212 4’6-二脒-2-苯胺,二盐酸盐 Cellstain- DAPI

荧光特性

λex=360 nm, λem=460 nm

操作说明

产品使用步骤 

1.用PBS或适当的缓冲液制备10-50μMDAPI溶液。a)

2.在细胞培养基中加入体积为细胞培养基1/10的DAPI溶液。b)

3.将细胞在37℃下孵育10-20分钟。

4.用PBS或适当的缓冲液清洗细胞两次。

5.使用带有360 nm激发光和460 nm发射滤光片的荧光显微镜观察细胞。

a)由于DAPI可能致癌,因此在处理过程中必须格外小心。

b)或者您可以用1/10浓度的DAPI缓冲溶液代替培养基。

注意事项

制备DAPI储备溶液时要用水(难溶于PBS)。

但是,那些溶解在水中的物质不适合长期储存。

考虑长期存储时,请使用解决方案类型产品-Cellstain®-DAPI解决方案(代码:D523),以提高解决方案的稳定性。

文献

1) W.Schnedl,A.V.Mikelsaar,M.Breitenbach and O.Dann,”DIPI and DAPI: Fluorescence Banding with Only Negligible Fading”,Hum. Genet.,1977,36,167.

2) I.W.Taylor and B.K.Milthorpe,”An Evaluation of DNA Fluochromes, Staining Techniques, and  Analysis for Flow Cytometry. I. Unperturebed Cellpopulations”,J.Histochem. Cytochem., 1980, 28(11), 1224.

3) F.Otto and K.G.Tsou,”A Comparative Study of DAPI,DIPI,and Hoechst 33258 and 33342 as  Chromosomal DNA Stains”, Stain Technol.,1985, 60, 7.

4) N.Poulin, A.Harrison and B.Palcic,”Quantitative Precision of an Automated Image Cytometric  System for the Measurement of DNA Content and Distribution in Cells Labeled  with Fluorescent Nucleic Acid Stains”, Cytometry, 1994,16,227.

5)M.Kawai,N.Yamaguchi and M.Nasu,”Rapid Enumeration of Physiologically Active Bacteria in  Purified Water Used in the Pharmaceutical Manufacturing Process”, J.Appl. Microbiol.,1999, 86 (3),496.

常见问题Q&A

Q1 核染色中所用染料的特征和区别是什么? 
 

 

 

A1:这里引入的染料具有通过与核酸相互作用而发出荧光或增加荧光强度的特性。

除荧光波长以外的差异如下。

[EB]

它没有碱基特异性,可与所有DNA和RNA结合。

它没有活细胞的膜通透性,并且可以染色死细胞。

[PI]

像EB一样,它没有基础特异性。它是一种更广泛使用的染料,因为插入时它的荧光强度比EB高。

它没有活细胞的膜通透性,并且可以染色死细胞。

[DAPI]

与双链小槽结合。它与腺嘌呤-胸苷簇具有高度结合。

它没有活细胞的膜通透性,并且可以染色死细胞。

[AO]

通过利用插入双链时和与单链磷酸结合时荧光波长不同的事实,可以在双链和单链之间进行区分。

它渗透到活细胞的细胞膜上。

[Hoechst33342,33258]

它与DNA的腺嘌呤胸苷部分特异性结合。

它是一种颜料,可以渗透到活细胞的细胞膜上并染色活细胞的DNA。

 

Q2:细胞染料的激发波长和发射波长是多少。

A2:

Cellstain- DAPI试剂货号:D212 4’6-二脒-2-苯胺,二盐酸盐 Cellstain- DAPI

 

 

Q3 如何使用DAPI进行核染色?

 

 

A3:有以下使用情况报告示例。制备DAPI储备溶液时要用水(难溶于PBS)。

但是,那些溶解在水中的物质不适合长期储存。

对于长期存储,请使用解决方案类型产品-Cellstain®-DAPI解决方案(货号:D523),具有更高的解决方案稳定性。使用时,用缓冲液或有机溶剂将储备溶液稀释至所需浓度。

[使用DAPI进行细胞染色的示例]

包含实体瘤或簇的样品的DAPI染色示例(参考文献3)

1)用0.02%胰蛋白酶-EDTA处理,在37°C下孵育30分钟,以1,500 rpm离心5分钟,然后冲洗上清液。

2)在-20°C下用50%甲醇(也可以使用乙醇)固定后,以1,500 rpm离心5分钟以除去上清液。

3)在-20℃下用30%甲醇洗涤后,以1,500rpm离心5分钟以除去上清液。

4)用0.2 M磷酸盐缓冲液(pH 7.2)洗涤后,以1,500 rpm离心5分钟以除去上清液。

5)每106个细胞添加0.2 mL的RNase的0.2 M磷酸盐缓冲液(pH 7.2)(pH 7.2)(1 mg / mL),并在37°C下孵育30分钟。

6)用0.2 M磷酸盐缓冲液(pH 7.2)洗涤后,以1,500 rpm离心5分钟以除去上清液。

7)加入10 mL 1μg/ mL DAPI溶液,并在黑暗中于4°C染色2小时。

[使用DAPI进行细胞染色的例子] Vollenweider等人在磷酸化因子激活的杀伤(LAK)细胞的细胞增殖试验中。

使用荧光打印机执行DAPI染色并进行测量(参考2)。

那时,请使用0.1 mg / mL的水溶液和1μg/ mL的甲醇溶液。

使用每孔100μL进行荧光染色。

日本同仁化学DSP试剂货号:D629 3,3′-二硫代二丙酸 二(N-羟基丁二酰亚胺酯) DSP| DOJINDO

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DSP试剂货号:D629
3,3′-二硫代二丙酸 二(N-羟基丁二酰亚胺酯)
DSP
商品信息
储存条件:0-5度保存,防潮
运输条件:室温
分子式:

C14H16N2O8S2

分子量:

404.42

SDS下载

选择规格:
1g

交联剂

日本同仁化学DTSSP试剂货号:D630 3,3′-二硫代双(磺酸琥珀酰亚氨基丙酸酯),二钠盐 DTSSP| DOJINDO

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DTSSP试剂货号:D630
3,3′-二硫代双(磺酸琥珀酰亚氨基丙酸酯),二钠盐
DTSSP
商品信息
储存条件:0-5度保存,防潮
运输条件:室温
分子式:

C14H14N2Na2O14S4

分子量:

608.51

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50mg

期货

 
交联剂

日本同仁化学DTCS Na试剂货号:D465 N-(二硫代羧基)肌氨酸,二钠盐,二水合物 DTCS Na| DOJINDO

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DTCS Na试剂货号:D465
N-(二硫代羧基)肌氨酸,二钠盐,二水合物
DTCS Na
商品信息
储存条件:0-5度保存,避光
运输条件:室温
分子式:

C4H5NNa2O2S2・2H2O

分子量:

245.23

SDS下载

选择规格:
100mg

期货

 
NO研究

关联产品

DTCS Na试剂货号:D465 N-(二硫代羧基)肌氨酸,二钠盐,二水合物 DTCS Na
ROS Assay Kit -Highly Sensitive DCFH-DA-试剂
ROS检测

氧化型/还原型谷胱甘肽定量试剂盒-GSSG/GSH Quantification Kit II
氧化型/还原型谷胱甘肽定量试剂盒

超氧化物歧化酶检测——SOD Assay Kit
超氧化物歧化酶(SOD)检测试剂盒-WST

二价铁离子检测探针—FerroOrange
细胞亚铁离子检测荧光探针

DTCS Na试剂货号:D465 N-(二硫代羧基)肌氨酸,二钠盐,二水合物 DTCS Na
Liperfluo-细胞脂质过氧化物检测
细胞脂质过氧化物检测试剂盒

在线小工具

实验工具|稀释计算器|摩尔浓度计算器

日本同仁化学2,3-Diaminonaphthalene(for NO detection)试剂货号:D418 2,3-二氨基萘| DOJINDO

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2,3-Diaminonaphthalene(for NO detection)试剂货号:D418
2,3-二氨基萘
2,3-Diaminonaphthalene(for NO detection)
商品信息
储存条件:-20度保存,避光
运输条件:室温
分子式:

C10H10N2

分子量:

158.2

SDS下载

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10mg

期货

 
NO研究

关联产品

2,3-Diaminonaphthalene(for NO detection)试剂货号:D418 2,3-二氨基萘
ROS Assay Kit -Highly Sensitive DCFH-DA-试剂
ROS检测

2,3-Diaminonaphthalene(for NO detection)试剂货号:D418 2,3-二氨基萘
氧化型/还原型谷胱甘肽定量试剂盒-GSSG/GSH Quantification Kit II
氧化型/还原型谷胱甘肽定量试剂盒

超氧化物歧化酶检测——SOD Assay Kit
超氧化物歧化酶(SOD)检测试剂盒-WST

二价铁离子检测探针—FerroOrange
细胞亚铁离子检测荧光探针

2,3-Diaminonaphthalene(for NO detection)试剂货号:D418 2,3-二氨基萘
Liperfluo-细胞脂质过氧化物检测
细胞脂质过氧化物检测试剂盒