Deoxycytidine triphosphate(Synonyms: dCTP; 2′-Deoxycytidine-5′-triphosphate)

生化分析试剂 Biochemical Assay Reagents
Deoxycytidine triphosphate;(Synonyms: dCTP; 2′-Deoxycytidine-5′-triphosphate)

Deoxycytidine triphosphate (dCTP) 是可用于 DNA 合成 (DNA synthesis) 的核苷三磷酸。Deoxycytidine triphosphate 可用于实时 PCR,cDNA 合成 和 DNA 测序等研究。

Deoxycytidine triphosphate(Synonyms: dCTP;  2′-Deoxycytidine-5′-triphosphate)

Deoxycytidine triphosphate Chemical Structure

CAS No. : 2056-98-6

规格 价格 是否有货 数量
10;mM;*;1 mL in Water ¥880 In-stock
10 mg ¥800 In-stock
50 mg ¥1800 询价
100 mg ; 询价 ;
200 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

Deoxycytidine triphosphate (dCTP) is a nucleoside triphosphate that can be used for DNA synthesis. Deoxycytidine triphosphate has many applications, such as real-time PCR, cDNA synthesis, and DNA sequencing[1][2][3].

IC50 Target

Human Endogenous Metabolite

;

体外研究
(In Vitro)

Assays of thirteen cell lines, derived from mouse lymphomas, myelomas, myeloid tumors, and a mastocytoma, for sensitivity to growth inhibition by 1-β-D-arabinofuranosylcytosine (ara-C) revealed a spectrum between the most and least sensitive which differed 100-fold from each other. An inverse correlation between sensitivity and cellular Deoxycytidine triphosphate (2′-Deoxycytidine-5′-triphosphate, dCTP) content is found, and this suggested that sensitivity of cells might be increased if the dCTP content was lowered during cell exposure to ara-C[1].
Deoxycytidine triphosphate trisodium salt is a phosphoethanolamine cytidylyltransferase substrate[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

467.16

Formula

C9H16N3O13P3

CAS 号

2056-98-6

中文名称

脱氧胞苷三磷酸;三磷酸脱氧胞苷;2′-脱氧胞苷-5′-三磷酸

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20deg;C 3 years
4deg;C 2 years
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

H2O : 50 mg/mL (107.03 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.1406 mL 10.7030 mL 21.4059 mL
5 mM 0.4281 mL 2.1406 mL 4.2812 mL
10 mM 0.2141 mL 1.0703 mL 2.1406 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. A W Harris, et al. Effect of thymidine on the sensitivity of cultured mouse tumor cells to 1-beta-D-arabinofuranosylcytosine. Cancer Res. 1979 Feb;39(2 Pt 1):538-41.

    [2]. B A Bladergroen, et al. CTP:phosphoethanolamine cytidylyltransferase. Biochim Biophys Acta. 1997 Sep 4;1348(1-2):91-9.

    [3]. M H Tattersall, et al. Deoxyribonucleoside triphosphates in human cells: changes in disease and following exposure to drugs. Eur J Clin Invest. 1975 Apr;5(2):191-202.

Deoxycytidine triphosphate trisodium salt(Synonyms: dCTP trisodium salt; 2′-Deoxycytidine-5′-triphosphate trisodium salt)

生化分析试剂 Biochemical Assay Reagents
Deoxycytidine triphosphate trisodium salt;(Synonyms: dCTP trisodium salt; 2′-Deoxycytidine-5′-triphosphate trisodium salt) 纯度: ge;97.0%

Deoxycytidine triphosphate trisodium salt (dCTP trisodium salt) 是可用于 DNA 合成 (DNA synthesis) 的核苷三磷酸。Deoxycytidine triphosphate trisodium salt 可用于实时 PCR,cDNA 合成 和 DNA 测序等研究。

Deoxycytidine triphosphate trisodium salt(Synonyms: dCTP trisodium salt; 2′-Deoxycytidine-5′-triphosphate trisodium salt)

Deoxycytidine triphosphate trisodium salt Chemical Structure

CAS No. : 109909-44-6

规格 价格 是否有货 数量
10 mg ¥800 In-stock
50 mg ¥1800 In-stock
100 mg ; 询价 ;
200 mg ; 询价 ;

* Please select Quantity before adding items.

Deoxycytidine triphosphate trisodium salt 相关产品

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  • Bioactive Compound Library Plus
  • Cell Cycle/DNA Damage Compound Library
  • Metabolism/Protease Compound Library
  • Anti-Aging Compound Library
  • Reprogramming Compound Library
  • Nucleotide Compound Library
  • Anti-Lung Cancer Compound Library
  • Anti-Blood Cancer Compound Library
  • Anti-Cancer Metabolism Compound Library

生物活性

Deoxycytidine triphosphate trisodium salt (dCTP trisodium salt) is a nucleoside triphosphate that can be used for DNA synthesis. Deoxycytidine triphosphate trisodium salt has many applications, such as real-time PCR, cDNA synthesis, and DNA sequencing[1][2][3].

体外研究
(In Vitro)

Assays of thirteen cell lines, derived from mouse lymphomas, myelomas, myeloid tumors, and a mastocytoma, for sensitivity to growth inhibition by 1-β-D-arabinofuranosylcytosine (ara-C) revealed a spectrum between the most and least sensitive which differed 100-fold from each other. An inverse correlation between sensitivity and cellular Deoxycytidine triphosphate (2′-Deoxycytidine-5′-triphosphate, dCTP) content is found, and this suggested that sensitivity of cells might be increased if the dCTP content was lowered during cell exposure to ara-C[1].
Deoxycytidine triphosphate trisodium salt is a phosphoethanolamine cytidylyltransferase substrate[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

533.10

Formula

C9H13N3Na3O13P3

CAS 号

109909-44-6

中文名称

脱氧胞苷三磷酸三钠盐;2′-脱氧胞苷-5′-三磷酸三钠盐

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, sealed storage, away from moisture

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro:;

H2O : 250 mg/mL (468.96 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.8758 mL 9.3791 mL 18.7582 mL
5 mM 0.3752 mL 1.8758 mL 3.7516 mL
10 mM 0.1876 mL 0.9379 mL 1.8758 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. A W Harris, et al. Effect of thymidine on the sensitivity of cultured mouse tumor cells to 1-beta-D-arabinofuranosylcytosine. Cancer Res. 1979 Feb;39(2 Pt 1):538-41.

    [2]. B A Bladergroen, et al. CTP:phosphoethanolamine cytidylyltransferase. Biochim Biophys Acta. 1997 Sep 4;1348(1-2):91-9.

    [3]. M H Tattersall, et al. Deoxyribonucleoside triphosphates in human cells: changes in disease and following exposure to drugs. Eur J Clin Invest. 1975 Apr;5(2):191-202.

2′-Deoxycytidine(Synonyms: Deoxycytidine; Cytosine deoxyriboside; Deoxyribose cytidine)

天然产物 糖类和糖苷 Saccharides and Glycosides

2′-Deoxycytidine;(Synonyms: Deoxycytidine; Cytosine deoxyriboside; Deoxyribose cytidine) 纯度: ge;98.0%

2′-Deoxycytidine,一个脱氧核苷,能够抑制 Brdu 的生物活性。

2

2′-Deoxycytidine Chemical Structure

CAS No. : 951-77-9

规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg) ; Apply now ;
10;mM;*;1 mL in DMSO ¥550 In-stock
100 mg ¥500 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

2′-Deoxycytidine 相关产品

bull;相关化合物库:

  • Covalent Screening Library Plus
  • Natural Product Library Plus
  • Drug Repurposing Compound Library Plus
  • Clinical Compound Library Plus
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  • Metabolism/Protease Compound Library
  • Natural Product Library
  • Clinical Compound Library
  • Human Endogenous Metabolite Compound Library
  • Drug Repurposing Compound Library
  • Covalent Screening Library
  • Nucleotide Compound Library
  • Gut Microbial Metabolite Library
  • Microbial Metabolite Library
  • Food-Sourced Compound Library

生物活性

2′-Deoxycytidine, a deoxyribonucleoside, could inhibit biological effects of Bromodeoxyuridine (Brdu).

IC50 Target[1]

Brdu

;

Human Endogenous Metabolite

;

Clinical Trial

分子量

227.22

Formula

C9H13N3O4

CAS 号

951-77-9

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

溶解性数据
In Vitro:;

DMSO : 50 mg/mL (220.05 mM; Need ultrasonic)

H2O : 50 mg/mL (220.05 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 4.4010 mL 22.0051 mL 44.0102 mL
5 mM 0.8802 mL 4.4010 mL 8.8020 mL
10 mM 0.4401 mL 2.2005 mL 4.4010 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;PBS

    Solubility: 110 mg/mL (484.11 mM); Clear solution; Need ultrasonic

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (11.00 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (11.00 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 3.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (11.00 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (11.00 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 4.

    请依序添加每种溶剂:;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.5 mg/mL (11.00 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (11.00 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Horn D, et al. Inhibition of biological effects of bromodeoxyuridine by deoxycytidine: correlation with decreased incorporation of bromodeoxyuridine into DNA. Somatic Cell Genet. 1976 Sep;2(5):469-81.

5-Methyl-2′-deoxycytidine(Synonyms: 5-Methyldeoxycytidine)

天然产物 糖类和糖苷 Saccharides and Glycosides

5-Methyl-2′-deoxycytidine;(Synonyms: 5-Methyldeoxycytidine) 纯度: 98.15%

5-Methyl-2′-deoxycytidine 在单链 DNA 中可发生顺式作用,使 DNA 从头甲基化。

5-Methyl-2

5-Methyl-2′-deoxycytidine Chemical Structure

CAS No. : 838-07-3

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ¥550 In-stock
100 mg ¥500 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

5-Methyl-2′-deoxycytidine 相关产品

bull;相关化合物库:

  • Covalent Screening Library Plus
  • Natural Product Library Plus
  • Bioactive Compound Library Plus
  • Epigenetics Compound Library
  • Metabolism/Protease Compound Library
  • Natural Product Library
  • Human Endogenous Metabolite Compound Library
  • Covalent Screening Library
  • Nucleotide Compound Library
  • Anti-Blood Cancer Compound Library
  • Transcription Factor Targeted Library
  • Food-Sourced Compound Library

生物活性

5-Methyl-2′-deoxycytidine in single-stranded DNA can act in cis to signal de novo DNA methylation[1][2].

IC50 Target

Human Endogenous Metabolite

;

分子量

241.24

Formula

C10H15N3O4

CAS 号

838-07-3

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

溶解性数据
In Vitro:;

DMSO : ≥ 100 mg/mL (414.52 mM)

H2O : 100 mg/mL (414.52 mM; Need ultrasonic)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 4.1452 mL 20.7262 mL 41.4525 mL
5 mM 0.8290 mL 4.1452 mL 8.2905 mL
10 mM 0.4145 mL 2.0726 mL 4.1452 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;PBS

    Solubility: 100 mg/mL (414.52 mM); Clear solution; Need ultrasonic

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (10.36 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (10.36 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 3.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (10.36 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (10.36 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 4.

    请依序添加每种溶剂:;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.5 mg/mL (10.36 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (10.36 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Christman JK, et al. 5-Methyl-2′-deoxycytidine in single-stranded DNA can act in cis to signal de novo DNA methylation. Proc Natl Acad Sci U S A. 1995 Aug 1;92(16):7347-51.

    [2]. Testillano PS, et al. The 5-methyl-deoxy-cytidine (5mdC) localization to reveal in situ the dynamics of DNA methylation chromatin pattern in a variety of plant organ and tissue cells during development. Physiol Plant. 2013 Sep;149(1):104-13.