Dermorphin Analog

Dermorphin Analog;

Dermorphin Analog 是 Dermorphin 的类似物。Dermorphin 为两栖类皮肤中发现的天然七肽,为 μ-阿片受体 (μ-opioid receptor) 激动剂。

Dermorphin Analogamp;;

Dermorphin Analog Chemical Structure

规格 价格 是否有货
1 mg ¥900 询问价格 货期
5 mg ¥1800 询问价格 货期
10 mg ¥2800 询问价格 货期

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生物活性

Dermorphin Analog is an analog of Dermorphin. Dermorphin is a natural heptapeptide μ-opioid receptor agonist found in amphibian skin.

分子量

901.43

Formula

C44H59N11O10

Sequence

Tyr-{d-Arg}-Phe-{SAR}-Tyr-Pro-Ser-NH2

Sequence Shortening

Y-{d-Arg}-F-{SAR}-YPS-NH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.

Dermorphin(Synonyms: 皮啡肽)

Dermorphin;(Synonyms: 皮啡肽) 纯度: 98.06%

Dermorphin 是在两栖类皮肤中发现的一种天然七肽 μ-阿片受体 (MOR) 激动剂。Dermorphin 可用于抑制神经痛。

Dermorphinamp;;(Synonyms: 皮啡肽)

Dermorphin Chemical Structure

CAS No. : 77614-16-5

规格 价格 是否有货 数量
10;mM;*;1 mL in Water ¥1590 In-stock
1 mg ¥600 In-stock
5 mg ¥1100 In-stock
10 mg ¥1800 In-stock
25 mg ¥3900 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

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Dermorphin 相关产品

bull;相关化合物库:

  • Natural Product Library Plus
  • Bioactive Compound Library Plus
  • GPCR/G Protein Compound Library
  • Neuronal Signaling Compound Library
  • Natural Product Library
  • Phenols Library
  • Neurotransmitter Receptor Compound Library
  • Peptide Library

生物活性

Dermorphin is a natural heptapeptide μ-opioid receptor (MOR) agonist found in amphibian skin. Inhibition of neuropathic pain[1].

IC50 Target

μ-opioid receptor (MOR)[1]

体外研究
(In Vitro)

Dermorphin, a peptide isolated from the skin of Phyllomedusa frogs and the peptide receptor (NOP) component by the endogenous agonist nociceptin/orphanin FQ (N/OFQ). In displacement binding studies at CHOhMu, Dermorphin and DeNo displac the binding of [3H]-DPN in a concentration dependent and saturable manner. Dermorphin displays an affinity of 7.17, while N/OFQ fails to displace [3H]-DPN at the Delta receptor. Dermorphin and DeNo stimulate the binding of GTPγ[35S] in a concentration dependent and saturable manner at the Mu receptor[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

802.87

Formula

C40H50N8O10

CAS 号

77614-16-5

Sequence

Tyr-{d-Ala}-Phe-Gly-Tyr-Pro-Ser-NH2

Sequence Shortening

Y-{d-Ala}-FGYPS-NH2

中文名称

皮啡肽

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

H2O : 120 mg/mL (149.46 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.2455 mL 6.2277 mL 12.4553 mL
5 mM 0.2491 mL 1.2455 mL 2.4911 mL
10 mM 0.1246 mL 0.6228 mL 1.2455 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Tiwari V, et al. Activation of Peripheral μ-opioid Receptors by Dermorphin [D-Arg2, Lys4] (1-4) Amide Leads to Modality-preferred Inhibition of Neuropathic Pain. Anesthesiology. 2016 Mar;124(3):706-20.

    [2]. Bird MF, et al. Characterisation of the Novel Mixed Mu-NOP Peptide Ligand Dermorphin-N/OFQ (DeNo). PLoS One. 2016 Jun 7;11(6):e0156897.

Kinase Assay
[2]

Membrane protein (40 μg) is incubated in 0.5 mL volume of 50 mM Tris, 0.2 mM EGTA, 1 mM MgCl2, 100 mM NaCl, 0.1% BSA, 0.15 mM bacitracin; pH 7.4, GDP (33 μM), and ∼150 pM GTPγ[35S]. Varying concentrations of reference ligands (Dermorphin, N/OFQ, Dynorphin-A and Leu-enkephalin) or DeNo (1 pM-10 μM) is added prior to incubation. Non-specific binding is determined in the presence of unlabeled GTPγS (10 μM). Samples are incubated at 30°C for 1 h with gentle agitation. Reactions are terminated by vacuum filtration through dry Whatman GF/B filters, using a Brandel harvester[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Tiwari V, et al. Activation of Peripheral μ-opioid Receptors by Dermorphin [D-Arg2, Lys4] (1-4) Amide Leads to Modality-preferred Inhibition of Neuropathic Pain. Anesthesiology. 2016 Mar;124(3):706-20.

    [2]. Bird MF, et al. Characterisation of the Novel Mixed Mu-NOP Peptide Ligand Dermorphin-N/OFQ (DeNo). PLoS One. 2016 Jun 7;11(6):e0156897.