Dynorphin A (Porcine, 9-17) 品牌:Anygen


Dynorphin A (Porcine, 9-17)

品牌:Anygen
CAS No.:
储存条件:-20℃
纯度:
产品编号

(生产商编号)

等级 规格 运输包装 零售价(RMB) 库存情况 参考值

303-90791

0.5 mg 咨询


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动,恕不另行通知,确切详情请联系上海金畔生物科技有限公司。

Dynorphin A (1-11)[D-Ala2](Porcine) 品牌:Anygen


Dynorphin A (1-11)[D-Ala2](Porcine)

品牌:Anygen
CAS No.:
储存条件:-20℃
纯度:
产品编号

(生产商编号)

等级 规格 运输包装 零售价(RMB) 库存情况 参考值

309-36953

0.5 mg 咨询


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动,恕不另行通知,确切详情请联系上海金畔生物科技有限公司。

Dynorphin A (Human, 1-13) 品牌:Peptide


Dynorphin A (Human, 1-13)

品牌:Peptide
CAS No.:72957-38-1
储存条件:-20℃
纯度:
产品编号

(生产商编号)

等级 规格 运输包装 零售价(RMB) 库存情况 参考值

337-40801

0.5mg 咨询


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动,恕不另行通知,确切详情请联系上海金畔生物科技有限公司。

Dynorphin A TFA(Synonyms: 肌啡肽A)

Dynorphin A TFA;(Synonyms: 肌啡肽A)

Dynorphin A TFA 是一种内源性阿片肽,是一种高效的 kappa 阿片受体 (KOR) 激活剂。Dynorphin A TFA 也作为其他阿片受体的激动剂,如 mu (MOR) 和 delta (DOR)。

Dynorphin A TFAamp;;(Synonyms: 肌啡肽A)

Dynorphin A TFA Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Dynorphin A TFA 的其他形式现货产品:

Dynorphin A

生物活性

Dynorphin A TFA, an endogenous opioid peptide, is a highy potent kappa opioid receptor (KOR) activator. Dynorphin A TFA also serve as an agonist for other opioid receptors,  such as mu (MOR) and delta (DOR)[1].

IC50 Target

Human Endogenous Metabolite

;

分子量

2261.50

Formula

C101H156F3N31O25

Sequence

Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys-Trp-Asp-Asn-Gln

Sequence Shortening

YGGFLRRIRPKLKWDNQ

中文名称

肌啡肽A

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Zhang, et al.Dynorphin A as a Potential Endogenous Ligand for Four Members of the Opioid Receptor Gene Family. J Pharmacol Exp Ther. 1998 Jul;286(1):136-41.

Dynorphin A (1-10)

Dynorphin A (1-10);

Dynorphin A (1-10) 是一种内源性的阿片样神经肽,与 κ 阿片受体 (κ-opioid receptor) 结合。Dynorphin A (1-10) 也阻断 NMDA 激活的电流,IC50 为 42.0 μM。

Dynorphin A (1-10)amp;;

Dynorphin A (1-10) Chemical Structure

CAS No. : 79994-24-4

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Dynorphin A (1-10) 的其他形式现货产品:

Dynorphin A (1-10) (TFA)

生物活性

Dynorphin A (1-10) an endogenous opioid neuropeptide, binds to extracellular loop 2 of the κ-opioid receptor. Dynorphin A (1-10) also blocks NMDA-activated current with an IC50 of 42.0 μM.

IC50 Target

κ-opioid receptor[1]
NMDA receptor[2]

体外研究
(In Vitro)

Dynorphin A (1-10) binds in the transmembrane domain of the κ-receptor[1]. The non-opioid actions of various forms of Dynorphin A (DynA) are examined on N-methyl-D-aspartate (NMDA) receptor channels in isolated rat trigeminal neurons using the whole-cell patch recording technique. All the dynorphins tested blocked NMDA-activated currents. The blocking actions are voltage-independent. The IC50 is 42.0 μM for DynA(1-10). To determine if shorter dynorphins have the similar blocking property, we examined the action of DynA(1-10) at different membrane potentials. DynA(1-10) blocks INMDA to a similar extent as the membrane potentials changed from -80 to +60 mV. Thus, despite a 160-fold difference in the apparent affinities, DynA(1-32) and DynA(1-10) both exert voltage-independent actions on NMDA receptors[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1234.45

Formula

C57H91N19O12

CAS 号

79994-24-4

Sequence

Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro

Sequence Shortening

YGGFLRRIRP

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Paterlini G, et al. Molecular simulation of dynorphin A-(1-10) binding to extracellular loop 2 of the kappa-opioidreceptor. A model for receptor activation. J Med Chem. 1997 Sep 26;40(20):3254-62.

    [2]. Chen L, et al. Dynorphin block of N-methyl-D-aspartate channels increases with the peptide length. J Pharmacol Exp Ther. 1998 Mar;284(3):826-31.

Dynorphin B (1-13)(Synonyms: 强啡肽 B (1-13))

Dynorphin B (1-13);(Synonyms: 强啡肽 B (1-13))

Dynorphin B (1-13) 是一种阿片样 κ 受体 (κ-opioid receptor) 激动剂。

Dynorphin B (1-13)amp;;(Synonyms: 强啡肽 B (1-13))

Dynorphin B (1-13) Chemical Structure

CAS No. : 83335-41-5

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Dynorphin B (1-13) 的其他形式现货产品:

Dynorphin B (1-13) (TFA)

生物活性

Dynorphin B (1-13) acts as an agonist on opioid κ-receptor.

IC50 Target

κ-opioid receptor[1]

体外研究
(In Vitro)

Dynorphin B (1-13), a 13 amino acid, is an extraordinarily potent opioid peptide. The neurophysiological actions of Dynorphin B (1-13) have been the subject of considerable research effort[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1570.84

Formula

C74H115N21O17

CAS 号

83335-41-5

Sequence

Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Gln-Phe-Lys-Val-Val-Thr

Sequence Shortening

YGGFLRRQFKVVT

中文名称

强啡肽 B (1-13)

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献
  • [1]. Chavkin C. Dynorphin–still an extraordinarily potent opioid peptide. Mol Pharmacol. 2013 Apr;83(4):729-36.

Dynorphin A (1-10) (TFA)

Dynorphin A (1-10) (TFA); 纯度: 99.43%

Dynorphin A (1-10) (TFA) 是一种内源性的阿片样神经肽,与 κ 阿片受体 (κ-opioid receptor) 结合。Dynorphin A (1-10) (TFA) 也阻断 NMDA 激活的电流,IC50 为 42.0 μM。

Dynorphin A (1-10) (TFA)amp;;

Dynorphin A (1-10) (TFA) Chemical Structure

规格 价格 是否有货 数量
1 mg ¥1100 In-stock
5 mg ¥3600 In-stock
10 mg ¥5600 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Dynorphin A (1-10) (TFA) 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Dynorphin A (1-10) (TFA), an endogenous opioid neuropeptide, binds to extracellular loop 2 of the κ-opioid receptor. Dynorphin A (1-10) (TFA) also blocks NMDA-activated current with an IC50 of 42.0 μM.

IC50 Target

κ-opioid receptor[1]
IC50: 42.0 μM (NMDA receptor)[2]

体外研究
(In Vitro)

Dynorphin A (1-10) (TFA), an endogenous opioid neuropeptide, binds in the transmembrane domain of the κ-receptor[1]. The non-opioid actions of various forms of Dynorphin A (DynA) are examined on N-methyl-D-aspartate (NMDA) receptor channels in isolated rat trigeminal neurons using the whole-cell patch recording technique. All the dynorphins tested blocked NMDA-activated currents. The blocking actions are voltage-independent. The IC50 is 42.0 μM for DynA(1-10). To determine if shorter dynorphins have the similar blocking property, we examined the action of DynA(1-10) at different membrane potentials. DynA(1-10) blocks INMDA to a similar extent as the membrane potentials changed from -80 to +60 mV. Thus, despite a 160-fold difference in the apparent affinities, DynA(1-32) and DynA(1-10) both exert voltage-independent actions on NMDA receptors[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1348.48

Formula

C59H92F3N19O14

Sequence

Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro

Sequence Shortening

YGGFLRRIRP

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro:;

DMSO : 100 mg/mL (74.16 mM; Need ultrasonic)

H2O : 50 mg/mL (37.08 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.7416 mL 3.7079 mL 7.4158 mL
5 mM 0.1483 mL 0.7416 mL 1.4832 mL
10 mM 0.0742 mL 0.3708 mL 0.7416 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (1.85 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (1.85 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (1.85 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (1.85 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂:;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.5 mg/mL (1.85 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (1.85 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Paterlini G, et al. Molecular simulation of dynorphin A-(1-10) binding to extracellular loop 2 of the kappa-opioidreceptor. A model for receptor activation. J Med Chem. 1997 Sep 26;40(20):3254-62.

    [2]. Chen L, et al. Dynorphin block of N-methyl-D-aspartate channels increases with the peptide length. J Pharmacol Exp Ther. 1998 Mar;284(3):826-31.

Porcine dynorphin A(1-13)(Synonyms: Dynorphin A Porcine Fragment 1-13)

Porcine dynorphin A(1-13);(Synonyms: Dynorphin A Porcine Fragment 1-13) 纯度: 99.61%

Porcine dynorphin A (1-13) 是一个有效的内源性阿片受体 (κ opioid receptor) 激动剂,在生理浓度下它有缓解疼痛作用。

Porcine dynorphin A(1-13)amp;;(Synonyms: Dynorphin A Porcine Fragment 1-13)

Porcine dynorphin A(1-13) Chemical Structure

CAS No. : 72957-38-1

规格 价格 是否有货 数量
1 mg ¥700 In-stock
5 mg ¥1500 In-stock
10 mg ¥2400 In-stock
25 mg ¥4900 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Porcine dynorphin A(1-13) 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Porcine dynorphin A (1-13) is a potent, endogenous κ opioid receptor agonist and is antinociceptive at physiological concentrations.

IC50 Target

Κ opioid receptor[1].

体内研究
(In Vivo)

Dynorphin A (1-13) exposure (33 μM) causes a significant loss in neuronal viability at 4 h with a visible destruction in neuronal morphology seen at 16 h. Exposure to dynorphin A (1-13) causes acute increases in [Ca2+]i in individual neurons similar to increases seen with acute NMDA treatment. Continuous exposure to dynorphin A (1-13) (100 μM) causes a significant loss of neurons over time[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1603.96

Formula

C75H126N24O15

CAS 号

72957-38-1

Sequence

Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys

Sequence Shortening

YGGFLRRIRPKLK

中文名称

强啡肽A(1-13),猪

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

H2O : ≥ 60 mg/mL (37.41 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.6235 mL 3.1173 mL 6.2346 mL
5 mM 0.1247 mL 0.6235 mL 1.2469 mL
10 mM 0.0623 mL 0.3117 mL 0.6235 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Hauser KF, et al. Dynorphin A (1-13) neurotoxicity in vitro: opioid and non-opioid mechanisms in mouse spinal cord neurons. Exp Neurol. 1999 Dec;160(2):361-75.

Dynorphin B (1-13) (TFA)(Synonyms: 强啡肽 B (1-13) 三氟乙酸盐)

Dynorphin B (1-13) (TFA);(Synonyms: 强啡肽 B (1-13) 三氟乙酸盐) 纯度: 99.52%

Dynorphin B (1-13) TFA 是一种阿片样 κ 受体 (κ-opioid receptor) 激动剂。

Dynorphin B (1-13) (TFA)amp;;(Synonyms: 强啡肽 B (1-13) 三氟乙酸盐)

Dynorphin B (1-13) (TFA) Chemical Structure

规格 价格 是否有货 数量
1 mg ¥1650 In-stock
5 mg ¥6600 In-stock
10 mg ¥9900 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Dynorphin B (1-13) (TFA) 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Dynorphin B (1-13) TFA acts as an agonist on opioid κ-receptor.

IC50 Target

κ-opioid receptor[1]

体外研究
(In Vitro)

Dynorphin B (1-13), a 13 amino acid, is an extraordinarily potent opioid peptide. The neurophysiological actions of Dynorphin B (1-13) have been the subject of considerable research effort[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1684.86

Formula

C76H116N21F3O19

Sequence

Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Gln-Phe-Lys-Val-Val-Thr

Sequence Shortening

YGGFLRRQFKVVT

中文名称

强啡肽 B (1-13) 三氟乙酸盐

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)

Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献
  • [1]. Chavkin C. Dynorphin–still an extraordinarily potent opioid peptide. Mol Pharmacol. 2013 Apr;83(4):729-36.

Dynorphin A

Dynorphin A; 纯度: 98.59%

Dynorphin A 是一种内源性阿片肽,是一种高效的 kappa 阿片受体 (KOR) 激活剂。Dynorphin A 也作为其他阿片受体的激动剂,如 mu (MOR) 和 delta (DOR)。

Dynorphin A 

Dynorphin A Chemical Structure

CAS No. : 80448-90-4

规格 价格 是否有货 数量
1 mg ¥700 In-stock
5 mg ¥2700 In-stock
10 mg ¥3700 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Dynorphin A 相关产品

相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Dynorphin A, an endogenous opioid peptide, is a highy potent kappa opioid receptor (KOR) activator. Dynorphin A also serve as an agonist for other opioid receptors,  such as mu (MOR) and delta (DOR)[1].

分子量

2147.48

Formula

C99H155N31O23

CAS 号

80448-90-4

Sequence

Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys-Trp-Asp-Asn-Gln

Sequence Shortening

YGGFLRRIRPKLKWDNQ

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Protect from light

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
In Vitro:;

DMSO : 120 mg/mL (55.88 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.4657 mL 2.3283 mL 4.6566 mL
5 mM 0.0931 mL 0.4657 mL 0.9313 mL
10 mM 0.0466 mL 0.2328 mL 0.4657 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 3 mg/mL (1.40 mM); Clear solution

    此方案可获得 ≥ 3 mg/mL (1.40 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 30.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 3 mg/mL (1.40 mM); Clear solution

    此方案可获得 ≥ 3 mg/mL (1.40 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 30.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂:;10% DMSO ;; 90% corn oil

    Solubility: ≥ 3 mg/mL (1.40 mM); Clear solution

    此方案可获得 ≥ 3 mg/mL (1.40 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 30.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Zhang, et al.Dynorphin A as a Potential Endogenous Ligand for Four Members of the Opioid Receptor Gene Family. J Pharmacol Exp Ther. 1998 Jul;286(1):136-41.