标签归档：emd

GRGDSPK(Synonyms: EMD 56574)

发表于由

GRGDSPK;(Synonyms: EMD 56574) 纯度: 98.30%

GRGDSPK (EMD 56574) 是包含精氨酸-甘氨酸-天冬氨酸 (RGD) 的多肽。 GRGDSPK 是一种竞争性且可逆的抑制肽，可抑制整联蛋白-纤连蛋白结合。GRGDSPK 可以用于研究整联蛋白在骨形成和吸收中的作用。

GRGDSPKamp;;(Synonyms: EMD 56574)

GRGDSPK Chemical Structure

CAS No. : 111119-28-9

规格 价格 是否有货 数量
5 mg ￥3800 In-stock
10 mg ￥6800 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

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GRGDSPK 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

GRGDSPK (EMD 56574) is a peptide incluing Arg-Gly-Asp (RGD). GRGDSPK (EMD 56574) is an competitive and reversible inhibitory peptide for inhibiting integrin-fibronectin binding. GRGDSPK is used to study the role of integrins in bone formation and resorption[1][2].

体外研究
(In Vitro)

GRGDSPK (EMD 56574; RGD; 0.1-50 μM; for 4 days) inhibits mineralization in a dose-dependent manner as determined by measuring calcium content and 70 bonehnit area of tissue in parietal bones 18 days old isolated from pregnant Sprague-Dawley rats[1].
GRGDSPK (10, 50 μM; for 4 days) dramatically alteres bone morphology, with a disruption of osteoblast and mineralized matrix organization[1].
GRGDSPK (RGD; 250 μM), added to the medium, effectively blocks integrin-fibronectin binding and significantly increases the average size of wild-type cell aggregates[2].
When GRGDSPK (250 μM) is added, wild-type mesendodermal progenitors exhibits strongly reduced adhesion forces and work, indicating that the detachment parameters recorded are specific for fibronectin and that integrins expressed in mesendodermal progenitors are involved[2].
GRGDSPK (RGD-containing, 1.5 mM, 1.0 mM, and 0.5 mM) and RGD-mod-ified peptides impair the ability of sperm to fertilizebovine oocytes, illustrated by a reduction in cleavage in a dose-dependent manner[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

715.76

Formula

C28H49N11O11
CAS 号

111119-28-9
Sequence

Gly-Arg-Gly-Asp-Ser-Pro-Lys
Sequence Shortening

GRGDSPK
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. G A Gronowicz, et al. Synthetic Peptide Containing Arg-Gly-Asp Inhibits Bone Formation and Resorption in a Mineralizing Organ Culture System of Fetal Rat Parietal Bones. J Bone Miner Res. 1994 Feb;9(2):193-201.

    [2]. Pierre-Henri Puech, et al. Measuring Cell Adhesion Forces of Primary Gastrulating Cells From Zebrafish Using Atomic Force Microscopy. J Cell Sci. 2005 Sep 15;118(Pt 18):4199-206.

    [3]. Sessions BR, et al. Effects of amino acid substitutions in and around the arginine-glycine-aspartic acid (RGD) sequence on fertilization and parthenogenetic development in mature bovine oocytes. Mol Reprod Dev. 2006 May;73(5):651-7.
Cell Assay
[1]

Proliferation assays are carried out in 48-well cell culture dishes coated with OCP or HL. In proliferation assays, 7.25×103 cells in 0.5 mL of culture medium are seeded in a 48-well dish. Culture medium is changed every 3 days. After 1, 6, 12, and 18 days of culture, plates are washed thrice with PBS to remove unattached cells, and attached cells are collected by trypsinization. The number of cells is counted using a hemocytometer.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. G A Gronowicz, et al. Synthetic Peptide Containing Arg-Gly-Asp Inhibits Bone Formation and Resorption in a Mineralizing Organ Culture System of Fetal Rat Parietal Bones. J Bone Miner Res. 1994 Feb;9(2):193-201.

    [2]. Pierre-Henri Puech, et al. Measuring Cell Adhesion Forces of Primary Gastrulating Cells From Zebrafish Using Atomic Force Microscopy. J Cell Sci. 2005 Sep 15;118(Pt 18):4199-206.

    [3]. Sessions BR, et al. Effects of amino acid substitutions in and around the arginine-glycine-aspartic acid (RGD) sequence on fertilization and parthenogenetic development in mature bovine oocytes. Mol Reprod Dev. 2006 May;73(5):651-7.

GRGDSPK TFA(Synonyms: EMD 56574 TFA)

发表于由

GRGDSPK TFA;(Synonyms: EMD 56574 TFA)

GRGDSPK TFA (EMD 56574 TFA) 是包含精氨酸-甘氨酸-天冬氨酸 (RGD) 的多肽。 GRGDSPK TFA 是一种竞争性且可逆的抑制肽，可抑制整联蛋白-纤连蛋白结合。GRGDSPK TFA 可以用于研究整联蛋白在骨形成和吸收中的作用。

GRGDSPK TFAamp;;(Synonyms: EMD 56574 TFA)

GRGDSPK TFA Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

GRGDSPK TFA 的其他形式现货产品:

GRGDSPK

生物活性

GRGDSPK TFA (EMD 56574 TFA) is a peptide incluing Arg-Gly-Asp (RGD). GRGDSPK TFA is an competitive and reversible inhibitory peptide for inhibiting integrin-fibronectin binding. GRGDSPK TFA is used to study the role of integrins in bone formation and resorption[1][2].

体外研究
(In Vitro)

GRGDSPK TFA (EMD 56574 TFA; RGD; 0.1-50 μM; for 4 days) inhibits mineralization in a dose-dependent manner as determined by measuring calcium content and 70 bonehnit area of tissue in parietal bones 18 days old isolated from pregnant Sprague-Dawley rats[1].
GRGDSPK TFA (10, 50 μM; for 4 days) dramatically alteres bone morphology, with a disruption of osteoblast and mineralized matrix organization[1].
GRGDSPK TFA (RGD; 250 μM), added to the medium, effectively blocks integrin-fibronectin binding and significantly increases the average size of wild-type cell aggregates[2].
When GRGDSPK TFA (250 μM) is added, wild-type mesendodermal progenitors exhibits strongly reduced adhesion forces and work, indicating that the detachment parameters recorded are specific for fibronectin and that integrins expressed in mesendodermal progenitors are involved[2].
GRGDSPK TFA (RGD-containing, 1.5 mM, 1.0 mM, and 0.5 mM) and RGD-mod-ified peptides impair the ability of sperm to fertilizebovine oocytes, illustrated by a reduction in cleavage in a dose-dependent manner[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

829.78

Formula

C30H50F3N11O13
Sequence

Gly-Arg-Gly-Asp-Ser-Pro-Lys
Sequence Shortening

GRGDSPK
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. G A Gronowicz, et al. Synthetic Peptide Containing Arg-Gly-Asp Inhibits Bone Formation and Resorption in a Mineralizing Organ Culture System of Fetal Rat Parietal Bones. J Bone Miner Res. 1994 Feb;9(2):193-201.

    [2]. Pierre-Henri Puech, et al. Measuring Cell Adhesion Forces of Primary Gastrulating Cells From Zebrafish Using Atomic Force Microscopy. J Cell Sci. 2005 Sep 15;118(Pt 18):4199-206.

    [3]. Sessions BR, et al. Effects of amino acid substitutions in and around the arginine-glycine-aspartic acid (RGD) sequence on fertilization and parthenogenetic development in mature bovine oocytes. Mol Reprod Dev. 2006 May;73(5):651-7.