CFSE(Synonyms: 5(6)-Carboxyfluorescein diacetate succinimidyl ester CFDA-SE 5(6)-CFDA N-succinmidyl ester)

发表于2023年8月28日由Whatman官网

CFSE;(Synonyms: 5(6)-Carboxyfluorescein diacetate succinimidyl ester; CFDA-SE; 5(6)-CFDA N-succinmidyl ester) 纯度: 99.01%

CFSE (5(6)-Carboxyfluorescein diacetate succinimidyl ester) 是一种细胞内荧光染料，可追踪细胞增殖。共价结合的 CFSE 在子细胞之间平均分配，从而可以区分连续的细胞分裂。

CFSEamp;;(Synonyms: 5(6)-Carboxyfluorescein diacetate succinimidyl ester; CFDA-SE; 5(6)-CFDA N-succinmidyl ester)

CFSE Chemical Structure

CAS No. : 150347-59-4

规格	价格	是否有货	数量
5 mg	￥800	In-stock
10 mg	￥1400	In-stock
50 mg	;	询价	;
100 mg	;	询价	;

* Please select Quantity before adding items.

CFSE 相关产品

bull;相关化合物库:

Bioactive Compound Library Plus

生物活性

CFSE (5(6)-Carboxyfluorescein diacetate succinimidyl ester) is an intracellular fluorescent dye which can track proliferating cells. Covalently bound CFSE is divided equally between daughter cells, allowing discrimination of successive rounds of cell division^[1]^[2].

体外研究
(In Vitro)

CFSE is a fluorescent dye which can track the cell division^[1]. To examine the ability of immune cells to migrate into live slices, a single-cell suspension of autologous splenocytes or PBMC is isolated, labeling them with CFSE, and adding them to the top of the slices on day 2 of culture. After 6 additional days of culture, the CFSE-labeling immune cells (green) are found to have migrated throughout the slices. No positive cells are found in the slices without the addition of CFSE-labeling immune cells^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

557.46

Formula

C₅₈H₃₈N₂O₂₂

CAS 号

150347-59-4

中文名称

5(6)-羧基二乙酸荧光素 N-琥珀酰亚胺酯

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)

溶解性数据

In Vitro:;

DMSO : 50 mg/mL (89.69 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.7939 mL	8.9693 mL	17.9385 mL
5 mM	0.3588 mL	1.7939 mL	3.5877 mL
10 mM	0.1794 mL	0.8969 mL	1.7939 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

Solubility: ≥ 2.08 mg/mL (3.73 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (3.73 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂：;10% DMSO ;; 90% corn oil

Solubility: ≥ 2.08 mg/mL (3.73 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (3.73 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。

参考文献

[1]. A Bruce Lyons, et al. Flow cytometric analysis of cell division by dilution of CFSE and related dyes. Curr Protoc Cytom. 2013;Chapter 9:Unit9.11.

[2]. Xiuyun Jiang et al. Long-lived pancreatic ductal adenocarcinoma slice cultures enable precise study of the immune microenvironment. Oncoimmunology. 2017; 6(7): e1333210.

Cell Assay ^[2]	Single-cell suspensions of splenocytes (or PBMC) are stained with 1 µM CFSE in PBS for 9 min at 37°C, combined with 20 mL of 10% FBS RPMI-1640 medium at RT for 2 min, centrifuged, washed, and counted. 2×10⁶ CFSE-labeling splenocytes are added to the top of the slices in 24-well membrane culture insert^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. A Bruce Lyons, et al. Flow cytometric analysis of cell division by dilution of CFSE and related dyes. Curr Protoc Cytom. 2013;Chapter 9:Unit9.11. [2]. Xiuyun Jiang et al. Long-lived pancreatic ductal adenocarcinoma slice cultures enable precise study of the immune microenvironment. Oncoimmunology. 2017; 6(7): e1333210.

Cell Assay
^[2]

Single-cell suspensions of splenocytes (or PBMC) are stained with 1 µM CFSE in PBS for 9 min at 37°C, combined with 20 mL of 10% FBS RPMI-1640 medium at RT for 2 min, centrifuged, washed, and counted. 2×10⁶ CFSE-labeling splenocytes are added to the top of the slices in 24-well membrane culture insert^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. A Bruce Lyons, et al. Flow cytometric analysis of cell division by dilution of CFSE and related dyes. Curr Protoc Cytom. 2013;Chapter 9:Unit9.11.

[2]. Xiuyun Jiang et al. Long-lived pancreatic ductal adenocarcinoma slice cultures enable precise study of the immune microenvironment. Oncoimmunology. 2017; 6(7): e1333210.

荧光染料Cy3 NHS ester(Synonyms: Cyanine3 NHS ester)

发表于2023年8月27日由Whatman官网

荧光染料Dye Reagents Cy3 NHS ester;(Synonyms: Cyanine3 NHS ester) 纯度: 99.05%

Cy3 NHS ester (Cyanine3 NHS ester) 是一种黄色荧光染料，用于标记生物分子中的氨基。常用于标记可溶性蛋白质、多肽和寡核苷酸/DNA。

Cy3 NHS esteramp;;(Synonyms: Cyanine3 NHS ester)

Cy3 NHS ester Chemical Structure

CAS No. : 1393363-07-9

规格	价格	是否有货	数量
10 mg	￥2800	In-stock
25 mg	￥4000	In-stock
50 mg	￥6900	In-stock
100 mg	;	询价	;
200 mg	;	询价	;

* Please select Quantity before adding items.

Cy3 NHS ester 相关产品

bull;相关化合物库:

Bioactive Compound Library Plus

生物活性

Cy3 NHS ester (Cyanine3 NHS ester) is a yellow emitting fluorescent dye for labeling amino-groups in biomolecules. Used to label soluble proteins, peptides, and oligonucleotides/DNA.

分子量

590.15

Formula

C₃₄H₄₀ClN₃O₄

CAS 号

1393363-07-9

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, protect from light, stored under argon

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light, stored under argon)

溶解性数据

In Vitro:;

DMSO : 250 mg/mL (423.62 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.6945 mL	8.4724 mL	16.9448 mL
5 mM	0.3389 mL	1.6945 mL	3.3890 mL
10 mM	0.1694 mL	0.8472 mL	1.6945 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light, stored under argon)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

1.

请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

Solubility: ≥ 2.08 mg/mL (3.52 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (3.52 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

Solubility: ≥ 2.08 mg/mL (3.52 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (3.52 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明

*以上所有助溶剂都可在 MCE 网站选购。

荧光染料Cy3 NHS ester(Synonyms: Cyanine3 NHS ester)

发表于2023年8月26日由Whatman官网

荧光染料Dye Reagents Cy3 NHS ester;(Synonyms: Cyanine3 NHS ester) 纯度: 99.05%

Cy3 NHS ester (Cyanine3 NHS ester) 是一种黄色荧光染料，用于标记生物分子中的氨基。常用于标记可溶性蛋白质、多肽和寡核苷酸/DNA。

Cy3 NHS ester Chemical Structure

CAS No. : 1393363-07-9

规格	价格	是否有货	数量
10 mg	￥2800	In-stock
25 mg	￥4000	In-stock
50 mg	￥6900	In-stock
100 mg	;	询价	;
200 mg	;	询价	;

* Please select Quantity before adding items.

Cy3 NHS ester 相关产品

bull;相关化合物库:

Bioactive Compound Library Plus

生物活性

Cy3 NHS ester (Cyanine3 NHS ester) is a yellow emitting fluorescent dye for labeling amino-groups in biomolecules. Used to label soluble proteins, peptides, and oligonucleotides/DNA.

分子量

590.15

Formula

C₃₄H₄₀ClN₃O₄

CAS 号

1393363-07-9

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, protect from light, stored under argon

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light, stored under argon)

溶解性数据

In Vitro:;

DMSO : 250 mg/mL (423.62 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.6945 mL	8.4724 mL	16.9448 mL
5 mM	0.3389 mL	1.6945 mL	3.3890 mL
10 mM	0.1694 mL	0.8472 mL	1.6945 mL

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

1.

请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

Solubility: ≥ 2.08 mg/mL (3.52 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (3.52 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

Solubility: ≥ 2.08 mg/mL (3.52 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (3.52 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明

*以上所有助溶剂都可在 MCE 网站选购。

荧光染料PB succiniMidyl ester(Synonyms: Ocean Blue, SE)

发表于2023年8月25日由Whatman官网

荧光染料Dye Reagents PB succiniMidyl ester;(Synonyms: Ocean Blue, SE)

PB succiniMidyl ester (Ocean Blue, SE) 是一种荧光团，激发/发射波长约为 405/455 nm。

PB succiniMidyl ester Chemical Structure

CAS No. : 215868-33-0

规格		是否有货
100 mg	;	询价	;
250 mg	;	询价	;
500 mg	;	询价	;

* Please select Quantity before adding items.

生物活性	PB succiniMidyl ester (Ocean Blue, SE) is a fluorophore with the λ_{excitation/emission} of ~405/455 nm^[1].
分子量	339.20
Formula	C₁₄H₇F₂NO₇
CAS 号	215868-33-0
运输条件	Room temperature in continental US; may vary elsewhere.
储存方式	Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献	[1]. Joachim Pfister, et al. Live-cell imaging with Aspergillus fumigatus-specific fluorescent siderophore conjugates. Sci Rep. 2020 Sep 23;10(1):15519.

Shanzhiside methyl ester(Synonyms: 山芝皂苷甲酯)

发表于2023年8月24日由Whatman官网

上海金畔生物科技有限公司提供天然产物萜类及其苷类Terpenoids and Glycosides。

Shanzhiside methyl ester (Synonyms: 山芝皂苷甲酯) 纯度: 98.57%

Shanzhiside methyl ester 是从轮状乳杆菌中分离得到的。Shanzhiside methyl ester 是一种小分子胰高血糖素样肽 1 (GLP-1 receptor) 受体激动剂，具有诱导抗异常性痛觉耐受的能力。

Shanzhiside methyl ester Chemical Structure

CAS No. : 64421-28-9

规格	价格	是否有货
5 mg	￥800	In-stock
10 mg	￥1400	In-stock
20 mg	￥2300	In-stock
50 mg		询价
100 mg		询价

* Please select Quantity before adding items.

Shanzhiside methyl ester 相关产品

•相关化合物库:

Natural Product Library Plus
Bioactive Compound Library Plus
GPCR/G Protein Compound Library
Natural Product Library
Glycoside Compound Library
Medicine Food Homology Compound Library
Terpenoids Library
Traditional Chinese Medicine Monomer Library
Microbial Metabolite Library
Anti-Parkinson’s Disease Compound Library
Neurodegenerative Disease-related Compound Library
Anti-Obesity Compound Library

生物活性

Shanzhiside methy lester is isolated from L. rotata. Shanzhiside methyl ester is a small molecule glucagon-like peptide-1 (GLP-1) receptor agonist and has the ability to induce anti-allodynic tolerance^[1].

IC₅₀ & Target

IC50: GLP-1 receptor^[1]

体内研究
(In Vivo)

Shanzhiside methyl ester exerts dose-dependent and long-lasting (>4 h) anti-allodynic effects in spinal nerve injury-induced neuropathic rats, with a maximal inhibition of 49% and a projected ED₅₀ of 40.4 μg^[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

406.38

Formula

C₁₇H₂₆O₁₁

CAS 号

64421-28-9

中文名称

山芝皂苷甲酯

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据

In Vitro:

DMSO : 100 mg/mL (246.08 mM; Need ultrasonic)

H₂O : 50 mg/mL (123.04 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.4608 mL	12.3038 mL	24.6075 mL
5 mM	0.4922 mL	2.4608 mL	4.9215 mL
10 mM	0.2461 mL	1.2304 mL	2.4608 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline

Solubility: ≥ 2.5 mg/mL (6.15 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (6.15 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)

Solubility: ≥ 2.5 mg/mL (6.15 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (6.15 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
3.

请依序添加每种溶剂： 10% DMSO 90% corn oil

Solubility: ≥ 2.5 mg/mL (6.15 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (6.15 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 Shanghai Jinpan Biotech Co Ltd 网站选购。

参考文献

[1]. Fan H, et al. Shanzhiside methylester, the principle effective iridoid glycoside from the analgesic herb Lamiophlomis rotata, reduces neuropathic pain by stimulating spinal microglial β-endorphin expression.Neuropharmacology. 2016 Feb;101:98-109.

荧光染料6-TAMRA-SE(Synonyms: 6-TAMRA-NHS ester 6-Carboxytetramethylrhodamine N-succinimidyl ester)

发表于2023年8月23日由Whatman官网

荧光染料Dye Reagents 6-TAMRA-SE;(Synonyms: 6-TAMRA-NHS ester; 6-Carboxytetramethylrhodamine N-succinimidyl ester) 纯度: ge;95.0%

6-TAMRA-SE (6-TAMRA-NHS ester) 是一种带有胺反应基的荧光染料。6-TAMRA-SE 是用于 DNA 自动测序的传统荧光团之一。

6-TAMRA-SEamp;;(Synonyms: 6-TAMRA-NHS ester; 6-Carboxytetramethylrhodamine N-succinimidyl ester)

6-TAMRA-SE Chemical Structure

CAS No. : 150810-69-8

规格	价格	是否有货	数量
10;mM;*;1 mL in DMSO	￥1625	In-stock
1 mg	￥600	In-stock
5 mg	￥1400	In-stock
10 mg	￥2000	In-stock
50 mg	￥7500	In-stock
100 mg	;	询价	;
200 mg	;	询价	;

* Please select Quantity before adding items.

6-TAMRA-SE 相关产品

bull;相关化合物库:

Bioactive Compound Library Plus

生物活性

6-TAMRA-SE (6-TAMRA-NHS ester) is a fluorescent dye carrying the amine reactive group. 6-TAMRA-SE is one of the traditional fluorophores used for automated DNA sequencing^[1]^[2]^[3].

体外研究
(In Vitro)

6-TAMRA-SE (6-TAMRA-NHS ester) is an amine-reactive form of tetramethylrhodamine. 6-TAMRA-SE (soluble in DMF and DMSO, absorption=546, emission=576 measured in MeOH ϵ=9.5×104ϵ=9.5×104)^[1]^[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

527.52

Formula

C₂₉H₂₅N₃O₇

CAS 号

150810-69-8

中文名称

6-羧基四甲基罗丹明琥珀酰亚胺酯

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)

溶解性数据

In Vitro:;

DMSO : 100 mg/mL (189.57 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.8957 mL	9.4783 mL	18.9566 mL
5 mM	0.3791 mL	1.8957 mL	3.7913 mL
10 mM	0.1896 mL	0.9478 mL	1.8957 mL

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

1.

请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

Solubility: 2.5 mg/mL (4.74 mM); Suspended solution; Need ultrasonic

此方案可获得 2.5 mg/mL (4.74 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。

以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

*以上所有助溶剂都可在 MCE 网站选购。

参考文献

[1]. Wang Y, et al. RNA molecules that specifically and stoichiometrically bind aminoglycoside antibiotics with high affinities. Biochemistry. 1996 Sep 24;35(38):12338-46.

[2]. Tessmar J, et al. Toward the development of biomimetic polymers by protein immobilization: PEGylation of insulin as a model reaction. Tissue Eng. 2004;10(3-4):441-453.

[3]. Thomas TP, et al. Investigation of tumor cell targeting of a dendrimer nanoparticle using a double-clad optical fiber probe. J Biomed Opt. 2008;13(1):014024.

荧光染料6-TAMRA-SE(Synonyms: 6-TAMRA-NHS ester 6-Carboxytetramethylrhodamine N-succinimidyl ester)

发表于2023年8月22日由Whatman官网

荧光染料Dye Reagents 6-TAMRA-SE;(Synonyms: 6-TAMRA-NHS ester; 6-Carboxytetramethylrhodamine N-succinimidyl ester) 纯度: ge;95.0%

6-TAMRA-SE (6-TAMRA-NHS ester) 是一种带有胺反应基的荧光染料。6-TAMRA-SE 是用于 DNA 自动测序的传统荧光团之一。

6-TAMRA-SE Chemical Structure

CAS No. : 150810-69-8

规格	价格	是否有货	数量
10;mM;*;1 mL in DMSO	￥1625	In-stock
1 mg	￥600	In-stock
5 mg	￥1400	In-stock
10 mg	￥2000	In-stock
50 mg	￥7500	In-stock
100 mg	;	询价	;
200 mg	;	询价	;

* Please select Quantity before adding items.

6-TAMRA-SE 相关产品

bull;相关化合物库:

Bioactive Compound Library Plus

生物活性

6-TAMRA-SE (6-TAMRA-NHS ester) is a fluorescent dye carrying the amine reactive group. 6-TAMRA-SE is one of the traditional fluorophores used for automated DNA sequencing^[1]^[2]^[3].

体外研究
(In Vitro)

6-TAMRA-SE (6-TAMRA-NHS ester) is an amine-reactive form of tetramethylrhodamine. 6-TAMRA-SE (soluble in DMF and DMSO, absorption=546, emission=576 measured in MeOH ϵ=9.5×104ϵ=9.5×104)^[1]^[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

527.52

Formula

C₂₉H₂₅N₃O₇

CAS 号

150810-69-8

中文名称

6-羧基四甲基罗丹明琥珀酰亚胺酯

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)

溶解性数据

In Vitro:;

DMSO : 100 mg/mL (189.57 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.8957 mL	9.4783 mL	18.9566 mL
5 mM	0.3791 mL	1.8957 mL	3.7913 mL
10 mM	0.1896 mL	0.9478 mL	1.8957 mL

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

1.

请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

Solubility: 2.5 mg/mL (4.74 mM); Suspended solution; Need ultrasonic

此方案可获得 2.5 mg/mL (4.74 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。

以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

*以上所有助溶剂都可在 MCE 网站选购。

参考文献

[1]. Wang Y, et al. RNA molecules that specifically and stoichiometrically bind aminoglycoside antibiotics with high affinities. Biochemistry. 1996 Sep 24;35(38):12338-46.

[2]. Tessmar J, et al. Toward the development of biomimetic polymers by protein immobilization: PEGylation of insulin as a model reaction. Tissue Eng. 2004;10(3-4):441-453.

[3]. Thomas TP, et al. Investigation of tumor cell targeting of a dendrimer nanoparticle using a double-clad optical fiber probe. J Biomed Opt. 2008;13(1):014024.

荧光染料Zinquin ethyl ester

发表于2023年8月21日由Whatman官网

荧光染料Dye Reagents Zinquin ethyl ester;

Zinquin ethyl ester 是一种锌离子特异性荧光团。 Zinquin ethyl ester 是 Zinquin 的荧光衍生物，具有亲脂性、锌敏感性，且能够穿透细胞膜。

Zinquin ethyl ester Chemical Structure

CAS No. : 181530-09-6

规格		是否有货
100 mg	;	询价	;
250 mg	;	询价	;
500 mg	;	询价	;

* Please select Quantity before adding items.

生物活性	Zinquin ethyl ester is a zinc ion-specific fluorophore. Zinquin ethyl ester is a lipophilic, zinc-sensitive, fluorescent derivative of Zinquin that is able to penetrate cell membranes^[1].
分子量	414.47
Formula	C₂₁H₂₂N₂O₅S
CAS 号	181530-09-6
运输条件	Room temperature in continental US; may vary elsewhere.
储存方式	Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献	[1]. Kevyn E Merten, et al. Zinc inhibits doxorubicin-activated calcineurin signal transduction pathway in H9c2 embryonic rat cardiac cells. Exp Biol Med (Maywood). 2007 May;232(5):682-9.

CY5-SE(Synonyms: Cy5 NHS Ester Sulfo-Cyanine5 Succinimidyl Ester)

发表于2023年8月20日由Whatman官网

CY5-SE;(Synonyms: Cy5 NHS Ester; Sulfo-Cyanine5 Succinimidyl Ester) 纯度: ge;98.0%

Cy5-SE (Cy5 NHS Ester) 是一种用于标记肽, 蛋白质, 寡核苷酸的氨基基团的反应染料。激发波长 (nm): 649, 发射波长 (nm): 670。

CY5-SEamp;;(Synonyms: Cy5 NHS Ester; Sulfo-Cyanine5 Succinimidyl Ester)

CY5-SE Chemical Structure

CAS No. : 146368-14-1

规格	价格	是否有货	数量
10;mM;*;1 mL in DMSO	￥1824	In-stock
5 mg	￥1500	In-stock
10 mg	￥2200	In-stock
25 mg	￥4400	In-stock
50 mg	;	询价	;
100 mg	;	询价	;

* Please select Quantity before adding items.

CY5-SE 相关产品

bull;相关化合物库:

Bioactive Compound Library Plus

生物活性

Cy5-SE (Cy5 NHS Ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reaction. This reagent is ideal for very cost-efficient labeling of soluble proteins, as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling. Excitation (nm):649, Emission (nm): 670.

体外研究
(In Vitro)

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Conjugation of SELP Analogues with Cy5-SE.
1. 1 mL of each 100 μg/mL SELP solution in 100 mM sodium bicarbonate buffer (pH 8.3) is mixed with 10 μL of 1.2 mg/mL Cy5 mono NHS-ester in 10% DMSO and incubates for 2 hours on ice.
2. To quench the reaction, 50 μL of 1 M Tris-HCl (pH 8.0) is added to the reaction solution.
3. Reaction mixtures are loaded onto 1.5 mL Sephadex G-25 columns, and Cy5-conjugated SELPs are eluted by centrifugation for 3 minutes at 1050g^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

753.88

Formula

C₃₇H₄₃N₃O₁₀S₂

CAS 号

146368-14-1

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)

溶解性数据

In Vitro:;

DMSO : 25 mg/mL (33.16 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.3265 mL	6.6324 mL	13.2647 mL
5 mM	0.2653 mL	1.3265 mL	2.6529 mL
10 mM	0.1326 mL	0.6632 mL	1.3265 mL

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

1.

请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

Solubility: ≥ 2.5 mg/mL (3.32 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (3.32 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

Solubility: ≥ 2.5 mg/mL (3.32 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (3.32 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明

*以上所有助溶剂都可在 MCE 网站选购。

参考文献

[1]. Jeon HY, et al. Array-Based High-Throughput Analysis of Silk-Elastinlike Protein Polymer Degradation and C-Peptide Release by Proteases. Anal Chem. 2016;88(10):5398-5405.

荧光染料Calcein tetraethyl ester(Synonyms: 钙黄绿素四乙酯 Fluorexon tetraethyl ester)

发表于2023年8月19日由Whatman官网

荧光染料Dye Reagents Calcein tetraethyl ester;(Synonyms: 钙黄绿素四乙酯; Fluorexon tetraethyl ester) 纯度: ge;98.0%

钙黄绿素四乙酯是一种是荧光染料, 用于生物学，因为它可以通过细胞膜进入活细胞，可用于细胞活力检测。

Calcein tetraethyl esteramp;;(Synonyms: 钙黄绿素四乙酯; Fluorexon tetraethyl ester)

Calcein tetraethyl ester Chemical Structure

CAS No. : 1170856-93-5

规格	价格	是否有货	数量
10;mM;*;1 mL in DMSO	￥1060	In-stock
10 mg	￥950	In-stock
25 mg	￥1800	In-stock
50 mg	￥2900	In-stock
100 mg	￥5200	In-stock
200 mg	;	询价	;
500 mg	;	询价	;

* Please select Quantity before adding items.

Calcein tetraethyl ester 相关产品

bull;相关化合物库:

Bioactive Compound Library Plus

生物活性

Calcein tetraethyl ester is a fluorescent dye, is used in biology as it can be transported through the cellular membrane into live cells, which makes it useful for testing of cell viability and for short-term labeling of cells.

分子量

734.75

Formula

C₃₈H₄₂N₂O₁₃

CAS 号

1170856-93-5

中文名称

钙黄绿素四乙酯；钙黄绿素四乙基酯

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

溶解性数据

In Vitro:;

DMSO : 25 mg/mL (34.03 mM; Need ultrasonic)

H₂O : < 0.1 mg/mL (insoluble)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.3610 mL	6.8050 mL	13.6101 mL
5 mM	0.2722 mL	1.3610 mL	2.7220 mL
10 mM	0.1361 mL	0.6805 mL	1.3610 mL

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

1.

请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

Solubility: ≥ 2.5 mg/mL (3.40 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (3.40 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

*以上所有助溶剂都可在 MCE 网站选购。

Cytiva思拓凡-Whatman滤纸滤膜滤器

沃特曼官网代理商|Whatman滤纸滤膜中国官网代理商

标签归档：ester

CFSE(Synonyms: 5(6)-Carboxyfluorescein diacetate succinimidyl ester CFDA-SE 5(6)-CFDA N-succinmidyl ester)

CFSE 相关产品

bull;相关化合物库:

荧光染料Cy3 NHS ester(Synonyms: Cyanine3 NHS ester)

Cy3 NHS ester 相关产品

bull;相关化合物库:

荧光染料Cy3 NHS ester(Synonyms: Cyanine3 NHS ester)

Cy3 NHS ester 相关产品

bull;相关化合物库:

荧光染料PB succiniMidyl ester(Synonyms: Ocean Blue, SE)

Shanzhiside methyl ester(Synonyms: 山芝皂苷甲酯)

Shanzhiside methyl ester 相关产品

•相关化合物库:

荧光染料6-TAMRA-SE(Synonyms: 6-TAMRA-NHS ester 6-Carboxytetramethylrhodamine N-succinimidyl ester)

6-TAMRA-SE 相关产品

bull;相关化合物库:

荧光染料6-TAMRA-SE(Synonyms: 6-TAMRA-NHS ester 6-Carboxytetramethylrhodamine N-succinimidyl ester)

6-TAMRA-SE 相关产品

bull;相关化合物库:

荧光染料Zinquin ethyl ester

CY5-SE(Synonyms: Cy5 NHS Ester Sulfo-Cyanine5 Succinimidyl Ester)

CY5-SE 相关产品

bull;相关化合物库:

荧光染料Calcein tetraethyl ester(Synonyms: 钙黄绿素四乙酯 Fluorexon tetraethyl ester)

Calcein tetraethyl ester 相关产品

bull;相关化合物库:

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