CFSE (5(6)-Carboxyfluorescein diacetate succinimidyl ester) is an intracellular fluorescent dye which can track proliferating cells. Covalently bound CFSE is divided equally between daughter cells, allowing discrimination of successive rounds of cell division[1][2].
体外研究 (In Vitro)
CFSE is a fluorescent dye which can track the cell division[1]. To examine the ability of immune cells to migrate into live slices, a single-cell suspension of autologous splenocytes or PBMC is isolated, labeling them with CFSE, and adding them to the top of the slices on day 2 of culture. After 6 additional days of culture, the CFSE-labeling immune cells (green) are found to have migrated throughout the slices. No positive cells are found in the slices without the addition of CFSE-labeling immune cells[2].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
557.46
Formula
C58H38N2O22
CAS 号
150347-59-4
中文名称
5(6)-羧基二乙酸荧光素 N-琥珀酰亚胺酯
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
-20deg;C, sealed storage, away from moisture and light
*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:;
DMSO : 50 mg/mL (89.69 mM; Need ultrasonic)
配制储备液
浓度溶剂体积质量
1 mg
5 mg
10 mg
1 mM
1.7939 mL
8.9693 mL
17.9385 mL
5 mM
0.3588 mL
1.7939 mL
3.5877 mL
10 mM
0.1794 mL
0.8969 mL
1.7939 mL
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
[1]. A Bruce Lyons, et al. Flow cytometric analysis of cell division by dilution of CFSE and related dyes. Curr Protoc Cytom. 2013;Chapter 9:Unit9.11.
[2]. Xiuyun Jiang et al. Long-lived pancreatic ductal adenocarcinoma slice cultures enable precise study of the immune microenvironment. Oncoimmunology. 2017; 6(7): e1333210.
Cell Assay [2]
Single-cell suspensions of splenocytes (or PBMC) are stained with 1 µM CFSE in PBS for 9 min at 37°C, combined with 20 mL of 10% FBS RPMI-1640 medium at RT for 2 min, centrifuged, washed, and counted. 2×106 CFSE-labeling splenocytes are added to the top of the slices in 24-well membrane culture insert[2].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献
[1]. A Bruce Lyons, et al. Flow cytometric analysis of cell division by dilution of CFSE and related dyes. Curr Protoc Cytom. 2013;Chapter 9:Unit9.11.
[2]. Xiuyun Jiang et al. Long-lived pancreatic ductal adenocarcinoma slice cultures enable precise study of the immune microenvironment. Oncoimmunology. 2017; 6(7): e1333210.
Cy3 NHS ester (Cyanine3 NHS ester) is a yellow emitting fluorescent dye for labeling amino-groups in biomolecules. Used to label soluble proteins, peptides, and oligonucleotides/DNA.
分子量
590.15
Formula
C34H40ClN3O4
CAS 号
1393363-07-9
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
-20deg;C, protect from light, stored under argon
*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light, stored under argon)
溶解性数据
In Vitro:;
DMSO : 250 mg/mL (423.62 mM; Need ultrasonic)
配制储备液
浓度溶剂体积质量
1 mg
5 mg
10 mg
1 mM
1.6945 mL
8.4724 mL
16.9448 mL
5 mM
0.3389 mL
1.6945 mL
3.3890 mL
10 mM
0.1694 mL
0.8472 mL
1.6945 mL
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light, stored under argon)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
Cy3 NHS ester (Cyanine3 NHS ester) is a yellow emitting fluorescent dye for labeling amino-groups in biomolecules. Used to label soluble proteins, peptides, and oligonucleotides/DNA.
分子量
590.15
Formula
C34H40ClN3O4
CAS 号
1393363-07-9
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
-20deg;C, protect from light, stored under argon
*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light, stored under argon)
溶解性数据
In Vitro:;
DMSO : 250 mg/mL (423.62 mM; Need ultrasonic)
配制储备液
浓度溶剂体积质量
1 mg
5 mg
10 mg
1 mM
1.6945 mL
8.4724 mL
16.9448 mL
5 mM
0.3389 mL
1.6945 mL
3.3890 mL
10 mM
0.1694 mL
0.8472 mL
1.6945 mL
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light, stored under argon)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
Shanzhiside methy lester is isolated from L. rotata. Shanzhiside methyl ester is a small molecule glucagon-like peptide-1 (GLP-1) receptor agonist and has the ability to induce anti-allodynic tolerance[1].
IC50 & Target
IC50: GLP-1 receptor[1]
体内研究 (In Vivo)
Shanzhiside methyl ester exerts dose-dependent and long-lasting (>4 h) anti-allodynic effects in spinal nerve injury-induced neuropathic rats, with a maximal inhibition of 49% and a projected ED50 of 40.4 μg[1].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
406.38
Formula
C17H26O11
CAS 号
64421-28-9
中文名称
山芝皂苷甲酯
运输条件
Room temperature in continental US; may vary elsewhere.
[1]. Fan H, et al. Shanzhiside methylester, the principle effective iridoid glycoside from the analgesic herb Lamiophlomis rotata, reduces neuropathic pain by stimulating spinal microglial β-endorphin expression.Neuropharmacology. 2016 Feb;101:98-109.
6-TAMRA-SE (6-TAMRA-NHS ester) 是一种带有胺反应基的荧光染料。6-TAMRA-SE 是用于 DNA 自动测序的传统荧光团之一。
6-TAMRA-SE Chemical Structure
CAS No. : 150810-69-8
规格
价格
是否有货
数量
10;mM;*;1 mL in DMSO
￥1625
In-stock
1 mg
￥600
In-stock
5 mg
￥1400
In-stock
10 mg
￥2000
In-stock
50 mg
￥7500
In-stock
100 mg
;
询价
;
200 mg
;
询价
;
* Please select Quantity before adding items.
6-TAMRA-SE 相关产品
bull;相关化合物库:
Bioactive Compound Library Plus
生物活性
6-TAMRA-SE (6-TAMRA-NHS ester) is a fluorescent dye carrying the amine reactive group. 6-TAMRA-SE is one of the traditional fluorophores used for automated DNA sequencing[1][2][3].
体外研究 (In Vitro)
6-TAMRA-SE (6-TAMRA-NHS ester) is an amine-reactive form of tetramethylrhodamine. 6-TAMRA-SE (soluble in DMF and DMSO, absorption=546, emission=576 measured in MeOH ϵ=9.5×104ϵ=9.5×104)[1][3].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
527.52
Formula
C29H25N3O7
CAS 号
150810-69-8
中文名称
6-羧基四甲基罗丹明琥珀酰亚胺酯
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
-20deg;C, sealed storage, away from moisture and light
*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:;
DMSO : 100 mg/mL (189.57 mM; Need ultrasonic)
配制储备液
浓度溶剂体积质量
1 mg
5 mg
10 mg
1 mM
1.8957 mL
9.4783 mL
18.9566 mL
5 mM
0.3791 mL
1.8957 mL
3.7913 mL
10 mM
0.1896 mL
0.9478 mL
1.8957 mL
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
[1]. Wang Y, et al. RNA molecules that specifically and stoichiometrically bind aminoglycoside antibiotics with high affinities. Biochemistry. 1996 Sep 24;35(38):12338-46.
[2]. Tessmar J, et al. Toward the development of biomimetic polymers by protein immobilization: PEGylation of insulin as a model reaction. Tissue Eng. 2004;10(3-4):441-453.
[3]. Thomas TP, et al. Investigation of tumor cell targeting of a dendrimer nanoparticle using a double-clad optical fiber probe. J Biomed Opt. 2008;13(1):014024.
6-TAMRA-SE (6-TAMRA-NHS ester) 是一种带有胺反应基的荧光染料。6-TAMRA-SE 是用于 DNA 自动测序的传统荧光团之一。
6-TAMRA-SE Chemical Structure
CAS No. : 150810-69-8
规格
价格
是否有货
数量
10;mM;*;1 mL in DMSO
￥1625
In-stock
1 mg
￥600
In-stock
5 mg
￥1400
In-stock
10 mg
￥2000
In-stock
50 mg
￥7500
In-stock
100 mg
;
询价
;
200 mg
;
询价
;
* Please select Quantity before adding items.
6-TAMRA-SE 相关产品
bull;相关化合物库:
Bioactive Compound Library Plus
生物活性
6-TAMRA-SE (6-TAMRA-NHS ester) is a fluorescent dye carrying the amine reactive group. 6-TAMRA-SE is one of the traditional fluorophores used for automated DNA sequencing[1][2][3].
体外研究 (In Vitro)
6-TAMRA-SE (6-TAMRA-NHS ester) is an amine-reactive form of tetramethylrhodamine. 6-TAMRA-SE (soluble in DMF and DMSO, absorption=546, emission=576 measured in MeOH ϵ=9.5×104ϵ=9.5×104)[1][3].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
527.52
Formula
C29H25N3O7
CAS 号
150810-69-8
中文名称
6-羧基四甲基罗丹明琥珀酰亚胺酯
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
-20deg;C, sealed storage, away from moisture and light
*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:;
DMSO : 100 mg/mL (189.57 mM; Need ultrasonic)
配制储备液
浓度溶剂体积质量
1 mg
5 mg
10 mg
1 mM
1.8957 mL
9.4783 mL
18.9566 mL
5 mM
0.3791 mL
1.8957 mL
3.7913 mL
10 mM
0.1896 mL
0.9478 mL
1.8957 mL
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
[1]. Wang Y, et al. RNA molecules that specifically and stoichiometrically bind aminoglycoside antibiotics with high affinities. Biochemistry. 1996 Sep 24;35(38):12338-46.
[2]. Tessmar J, et al. Toward the development of biomimetic polymers by protein immobilization: PEGylation of insulin as a model reaction. Tissue Eng. 2004;10(3-4):441-453.
[3]. Thomas TP, et al. Investigation of tumor cell targeting of a dendrimer nanoparticle using a double-clad optical fiber probe. J Biomed Opt. 2008;13(1):014024.
Zinquin ethyl ester is a zinc ion-specific fluorophore. Zinquin ethyl ester is a lipophilic, zinc-sensitive, fluorescent derivative of Zinquin that is able to penetrate cell membranes[1].
分子量
414.47
Formula
C21H22N2O5S
CAS 号
181530-09-6
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献
[1]. Kevyn E Merten, et al. Zinc inhibits doxorubicin-activated calcineurin signal transduction pathway in H9c2 embryonic rat cardiac cells. Exp Biol Med (Maywood). 2007 May;232(5):682-9.
Cy5-SE (Cy5 NHS Ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reaction. This reagent is ideal for very cost-efficient labeling of soluble proteins, as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling. Excitation (nm):649, Emission (nm): 670.
体外研究 (In Vitro)
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Conjugation of SELP Analogues with Cy5-SE. 1. 1 mL of each 100 μg/mL SELP solution in 100 mM sodium bicarbonate buffer (pH 8.3) is mixed with 10 μL of 1.2 mg/mL Cy5 mono NHS-ester in 10% DMSO and incubates for 2 hours on ice. 2. To quench the reaction, 50 μL of 1 M Tris-HCl (pH 8.0) is added to the reaction solution. 3. Reaction mixtures are loaded onto 1.5 mL Sephadex G-25 columns, and Cy5-conjugated SELPs are eluted by centrifugation for 3 minutes at 1050g[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
753.88
Formula
C37H43N3O10S2
CAS 号
146368-14-1
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
-20deg;C, sealed storage, away from moisture and light
*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:;
DMSO : 25 mg/mL (33.16 mM; Need ultrasonic)
配制储备液
浓度溶剂体积质量
1 mg
5 mg
10 mg
1 mM
1.3265 mL
6.6324 mL
13.2647 mL
5 mM
0.2653 mL
1.3265 mL
2.6529 mL
10 mM
0.1326 mL
0.6632 mL
1.3265 mL
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献
[1]. Jeon HY, et al. Array-Based High-Throughput Analysis of Silk-Elastinlike Protein Polymer Degradation and C-Peptide Release by Proteases. Anal Chem. 2016;88(10):5398-5405.
