Omiganan-FITC TFA

Omiganan-FITC TFA;

Omiganan-FITC TFA 是由 Omiganan 和 FITC 组成的肽-FITC 复合体。Omiganan 是一种抗细菌和真菌的阳离子肽,可作为一种局部凝胶用于预防导管相关感染。

Omiganan-FITC TFAamp;;

Omiganan-FITC TFA Chemical Structure

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生物活性

Omiganan-FITC TFA is a peptide-FITC complex composed of Omiganan and a FITC. Omiganan is a bactericidal and fungicidal cationic peptide being developed as a topical gel for prevention of catheter-associated infections[1].

Sequence

FITC-Ile-Leu-Arg-Trp-Pro-Trp-Trp-Pro-Trp-Arg-Arg-Lys-NH2

Sequence Shortening

FITC-ILRWPWWPWRRK-NH2-FITC

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Thomas R Fritsche, et al. Antimicrobial Activity of Omiganan Pentahydrochloride Against Contemporary Fungal Pathogens Responsible for Catheter-Associated Infections. Antimicrob Agents Chemother. 2008 Mar;52(3):1187-9.

日本同仁化学FITC-I试剂货号:F007 FITC-I试剂 CAS号:3326-32-7| DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

FITC-I试剂货号:F007
FITC-I试剂
CAS号:3326-32-7
商品信息
储存条件:0-5度保存,避光防潮
运输条件:室温
分子式:

C21H11NO5S

分子量:

389.38

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100mg

期货

 

关联产品

FITC-I试剂货号:F007 FITC-I试剂 CAS号:3326-32-7
Biotin Labeling Kit – NH2试剂盒
生物素抗体标记试剂盒-氨基

Peroxidase Labeling Kit – NH2试剂盒
过氧化物酶标记试剂盒-氨基

Biotin Labeling Kit – NH2 (for 1mg)试剂盒
生物素标记试剂盒-氨基

Fluorescein Labeling Kit – NH2试剂盒
荧光素标记试剂盒-氨基

Isothiocyanobenzyl-EDTA试剂
Isothiocyanobenzyl-EDTA

日本同仁化学细胞凋亡检测试剂盒——Annexin V, FITC Apoptosis Detection Kit货号:AD10| DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

细胞凋亡检测试剂盒——Annexin V, FITC Apoptosis Detection Kit货号:AD10
Annexin V-FITC/PI凋亡检测试剂盒
Annexin V, FITC Apoptosis Detection Kit
商品信息
储存条件:0-5度保存,避光
运输条件:室温

特点:

· 灵敏度&稳定性高

· 性价比高

· 荧光显微镜和流式检测双重方法检测

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产品文献
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选择规格:
50assays
50assays*2

价格:

细胞凋亡检测

细胞凋亡检测试剂盒——Annexin V, FITC Apoptosis Detection Kit货号:AD10

细胞凋亡检测试剂盒——Annexin V, FITC Apoptosis Detection Kit货号:AD10

活动进行中
试剂盒内含
产品概述
原理
荧光参数
相关资料
常见问题Q&A
参考文献

活动进行中

订购满5000元,200元礼品等你拿

凑单关联产品TOP5

NO.1.    Cell Counting Kit-8     细胞增殖毒性检测   

NO.2.    PI/RNase Staining    细胞周期检测   

NO.3.    Caspase-3 Assay Kit -Colorimetric    细胞凋亡检测  

NO.4.    ROS Assay Kit    活性氧检测

NO.5.    Calcein-AM/PI细胞双染试剂盒    活死细胞双染 

试剂盒内含

细胞凋亡检测试剂盒——Annexin V, FITC Apoptosis Detection Kit货号:AD10

产品概述

细胞凋亡是指为维持有机体内环境稳定,由基因控制的细胞自主的有序的死亡。正常情况下任何细胞在形成过程中发生的异常都会通过凋亡消除。例如体内的癌细胞增长为肿瘤的过程会受细胞凋亡的引导而被抑制。然而在抑癌基因p53出现问题时,凋亡就不会诱导发生,从而导致癌细胞的不断增长。细胞凋亡可以通过细胞形态的变化或生物化学的变化来检测。目前常用的指标有caspase活性变化、DNA碎片、磷脂酰丝氨酸的外翻等。

原理

Annexin V染色的细胞可以用于检测细胞凋亡早期的细胞膜变化。在细胞凋亡早期,膜磷脂酰丝氨酸由脂膜内侧翻向外侧。Annexin V是一种分子量为35-36kD的Ca2+依赖性磷脂结合蛋白,与磷脂酰丝氨酸有高度亲和力,可通过细胞外侧暴露的磷脂酰丝氨酸与凋亡早期细胞的胞膜特异性结合,因此Annexin V 被作为检测细胞早期凋亡的灵敏指标之一。用绿色荧光FITC标记的Annexin V通过流式细胞仪或荧光显微镜可以检测到细胞凋亡的发生。碘化丙啶 (Propidium Iodide, PI) 是一种核酸染料,PI只能透过凋亡晚期和死细胞的细胞膜,因此Annexin V和PI结合使用,可以区分凋亡早晚期的细胞及死细胞。

细胞凋亡检测试剂盒——Annexin V, FITC Apoptosis Detection Kit货号:AD10

Normal Cell                         Early stages of apoptosis                   Late stages of apoptosis

荧光参数

-流式细胞仪检测

1. 加样到流式细胞仪检测,激发波长Ex = 488 nm;发射波长Em = 530 nm。

2. Annexin V-FITC的绿色荧光通过FITC通道 (FL1) 检测;PI的红色荧光通过PI 通道 (FL2或FL3) 检测 (建议使用FL3)。

-荧光显微镜检测

将细胞悬液滴到玻片上,置于显微镜上使用合适的滤光片检测。* 由于EDTA会对细胞膜的特定部位有损伤,建议使用不含EDTA的胰酶消化贴壁细胞。

相关资料

所需的设备和材料

– 样品和诱导剂

– PBS、超纯水

– 合适量程的移液器

– 细胞培养用6,12,24,96孔板

– 流式细胞仪或荧光显微镜。

* 最大激发/发射波长  Annexin V, FITC: 494 nm/518 nm;PI: 535 nm/617 nm

溶液制备

1×Annexin V Binding solution:将10×Annexin V Binding Buffer用超纯水稀释10倍。

稳定性

试剂盒在0-5℃下可以保存6个月,请避光保存Annexin V, FITC结合物。

*实验实例及操作步骤请参考中文说明书

常见问题Q&A

Q1:贴壁细胞凋亡实验是否能够不消化?
A1:不选择消化的原因是什么?    =>主要是担心胰酶对细胞膜损伤而影响结果判断。

贴壁细胞在检测凋亡之前,如果没有出现团聚的情况,那么是可以尝试不消化直接镜检。理由是基于Annexin V- FITC与细胞反应的一个机理,凋亡早期的膜磷脂酰丝氨酸由膜内外翻,与Annexin V特异性结合,那么FITC标记的Annexin V呈现一个绿色荧光,所以我们保证足够的细胞膜暴露在工作液中,那即使没有经过胰酶消化,最终也是能够用荧光显微镜观察凋亡的情况。

 

不消化有几点好的地方:

1.避免一个对细胞膜的损伤,那结果能够很好的反应促凋亡的结果

2.操作简便,避免消化以及清洗过程中出现的操作失误

 

不消化有几点担心的地方:

1.无法制成细胞悬液,染色可能出现不充分

2.可能无法观察凋亡晚期PI和FITC双染的细胞

Q2:目前用Tunel的检测试剂盒做凋亡实验。和Tunel比较,用Annexin V-FITC的优点是什么?
A2: TUNEL这种方法灵敏度很高,但是特异性比较低,只能标记出凋亡中期和凋亡晚期的细胞,而且细胞坏死也会发生DNA断裂,不能算是真正的凋亡细胞,造成假阳性的现象。    建议老师两种方法都进行,以Tunel方法为主,Annexin V的方法为辅,这样两种方法都进行,互相也可以验证结果是否准确,互作参考。

参考文献

1. A Novel Allosteric Inhibitor of Phosphoglycerate Mutase 1 Suppresses Growth and Metastasis of Non-Small-Cell Lung Cancer,

Cell Metabolism, 2019, 30, 1-13

2. Tumor Microenvironment Activable Self-Assembled DNA Hybrids for pH and Redox Dual-Responsive Chemotherapy/PDT

Treatment of Hepatocellular Carcinoma, Advanced Science, 2017, 4(4), 1600460

3. Biogenic (R)-(+)-Lipoic Acid Only Constructed Cross-LinkedVesicles with Synergistic Anticancer Potency,

Advanced Functional Materials, 2018, 1806567

4. Anti-mitotic chemotherapeutics promote apoptosis through TL1A-activated death receptor 3 in cancer cells,

Cell Research, 2018, 28, 544-555

5. Bacteria-Driven Hypoxia Targeting for Combined Biotherapy and Photothermal Therapy, ACS Nano, 2018, 12(6), 5995-6005

6. Nanoenzyme-Augmented Cancer Sonodynamic Therapy by Catalytic Tumor Oxygenation, ACS Nano, 2018, 12, 3780-3795

7. Tumor-Specific Multiple Stimuli-Activated Dendrimeric Nanoassemblies with Metabolic Blockade Surmount Chemotherapy

Resistance, ACS Nano, 2017, 11(1), 416-429

8. Near Infrared-Guided Smart Nanocarriers for MicroRNA-Controlled Release of Doxorubicin/siRNA with Intracellular ATP as Fuel,

ACS Nano, 2016, 10(3), 3637-47

9. Noninvasive small-animal imaging of galectin-1 upregulation for predicting tumor resistance to radiotherapy,

Biomaterials, 2018, 158, 1-9

10. Cytotoxicity of guanine-based degradation products contributes to the antiproliferative activity of guanine-rich oligonucleotides,Chemical Science, 2015, 6, 3834-3836

11. A Novel Strategy through Combining iRGD Peptide with Tumor-icroenvironment-Responsive and Multistage Nanoparticles

for Deep Tumor Penetration, ACS Appl.Mater.Interfaces, 2015, 7(49), 27458-27466

12. JOSD1 inhibits mitochondrial apoptotic signalling to drive acquired chemoresistance in gynaecological cancer by stabilizing MCL1,Cell Death & Differentiation, 2020, 27, 55-70

13. The influence of printing parameters on cell survival rate and printability in microextrusion-based 3D cell printing technology,Biofabrication, 2015, 7(4), 045002

14. Downregulation of MCL-1 and upregulation of PUMA using mTOR inhibitors enhance antitumor efficacy of BH3 mimetics in triple-negative breast cancer, Cell Death & Disease, 2018, 9, 137

15. Mutations in the P10 region of procaspase-8 lead to chemotherapy resistance in acute myeloid leukemia by impairing procaspase-8 dimerization, Cell Death & Disease, 2018, 9(5), 516

16. Preclinical evaluation of antitumor of the proteasome inhibitor MLN2238 (ixazomib) in hepatocellular carcinoma cells,Cell Death & Disease, 2018, 9, 28

17. Long Noncoding RNA HCAL Facilitates the Growth and Metastasis of Hepatocellular Carcinoma by Acting as a ceRNA of LAPTM4B,Molecular Therapy: Nucleic Acids, 2017, 9, 440-451

18. TMEM74 promotes tumor cell survival by inducing autophagy via interactions with ATG16L1 and ATG9A,Cell Death & Disease, 2017, 8, e3031-3045

19. Nogo-C regulates cardiomyocyte apoptosis during mouse myocardial infarction, Cell Death & Disease, 2016, 7, e2432

20. TonEBP modulates the protective effect of taurine in ischemia-induced cytotoxicity in cardiomyocytes,Cell Death & Disease, 2015, 6, e2025

关联产品

细胞凋亡检测试剂盒——Annexin V, FITC Apoptosis Detection Kit货号:AD10
Caspase-3 Assay Kit -Colorimetric-试剂盒
Caspase-3检测试剂盒-比色法

细胞凋亡检测试剂盒——Annexin V, FITC Apoptosis Detection Kit货号:AD10
细胞凋亡检测试剂盒——Annexin V, 633 Apoptosis Detection Kit
Annexin V, 633/PI凋亡检测试剂盒

在线小工具

实验工具|稀释计算器|摩尔浓度计算器

FITC(Synonyms: 异硫氰酸荧光素酯 Fluorescein 5-isothiocyanate)

FITC;(Synonyms: 异硫氰酸荧光素酯; Fluorescein 5-isothiocyanate) 纯度: 98.77%

FITC (Fluorescein 5-isothiocyanate) 是一种用于胺标记的荧光探针。FITC 是对 pH 和 Cu2+ 敏感的荧光染料。

FITCamp;;(Synonyms: 异硫氰酸荧光素酯; Fluorescein 5-isothiocyanate)

FITC Chemical Structure

CAS No. : 3326-32-7

规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg) ; Apply now ;
10;mM;*;1 mL in DMSO ¥550 In-stock
100 mg ¥500 In-stock
500 mg ¥990 In-stock
1 g ¥1683 In-stock
5 g ; 询价 ;
10 g ; 询价 ;

* Please select Quantity before adding items.

FITC 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

FITC (Fluorescein 5-isothiocyanate) is a fluorescence probe for the labeling of amines. FITC is a pH- and Cu2+-sensitive fluorescence dye[1][2].

体外研究
(In Vitro)

Annexin V-FITC/PI is employed to detect chondrocyte apoptosis[1]. The PEI-modified CDs are conjugated with fluorescein isothiocyanate (FITC) to fabricate CDs-FITC composites[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

389.38

Formula

C21H11NO5S

CAS 号

3326-32-7

中文名称

异硫氰酸荧光素酯;荧光素-5-异氰酸酯

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, protect from light, stored under nitrogen

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light, stored under nitrogen)

溶解性数据
In Vitro:;

DMSO : 83.33 mg/mL (214.01 mM; Need ultrasonic)

H2O : < 0.1 mg/mL (insoluble)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.5682 mL 12.8409 mL 25.6819 mL
5 mM 0.5136 mL 2.5682 mL 5.1364 mL
10 mM 0.2568 mL 1.2841 mL 2.5682 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.08 mg/mL (5.34 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (5.34 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: 2.08 mg/mL (5.34 mM); Suspended solution; Need ultrasonic

    此方案可获得 2.08 mg/mL (5.34 mM) 的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Gao SG, et al. Phosphorylation of osteopontin has proapoptotic and proinflammatory effects on human knee osteoarthritis chondrocytes. Exp Ther Med. 2016 Nov;12(5):3488-3494. Epub 2016 Oct 5.

    [2]. Zhu X, et al. Ratiometric, visual, dual-signal fluorescent sensing and imaging of pH/copper ions in real samples based on carbon dots-fluorescein isothiocyanate composites. Talanta. 2017 Jan 1;162:65-71

Cell Assay
[1]

The frequency of chondrocyte apoptosis is measured by flow cytometry with Annexin V-fluorescein isothiocyanate (FITC) and propidium iodide. A total of 1×104 treated chondrocytes are collected from each group, washed in cold PBS and incubated with Annexin V-FITC and PI at room temperature for 15 min in the dark on ice. These samples are then analyzed using a fluorescence-activated cell sorter. Cell Quest software is used to analyze the percentage of apoptosis. All tests are repeated in triplicate.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Gao SG, et al. Phosphorylation of osteopontin has proapoptotic and proinflammatory effects on human knee osteoarthritis chondrocytes. Exp Ther Med. 2016 Nov;12(5):3488-3494. Epub 2016 Oct 5.

    [2]. Zhu X, et al. Ratiometric, visual, dual-signal fluorescent sensing and imaging of pH/copper ions in real samples based on carbon dots-fluorescein isothiocyanate composites. Talanta. 2017 Jan 1;162:65-71

FITC-Dextran (MW 10000)(Synonyms: 荧光素异硫氰酸酯-葡聚糖(MW 10000))

FITC-Dextran (MW 10000);(Synonyms: 荧光素异硫氰酸酯-葡聚糖(MW 10000))

FITC-Dextran (MW 10000) 是由 FITC 偶联葡聚糖形成的一种标记物 (10 kDa)。FITC-Dextran 是由不同长度的支链葡萄糖分子组成的被标记的多糖,可根据所使用的葡聚糖的大小来测定血脑屏障的溶质、离子和蛋白质渗透性。

FITC-Dextran (MW 10000)amp;;(Synonyms: 荧光素异硫氰酸酯-葡聚糖(MW 10000))

FITC-Dextran (MW 10000) Chemical Structure

CAS No. : 60842-46-8

规格 价格 是否有货 数量
5 mg ¥500 In-stock
10 mg ¥850 In-stock
50 mg ¥2500 In-stock
100 mg ; 询价 ;
200 mg ; 询价 ;

* Please select Quantity before adding items.

FITC-Dextran (MW 10000) 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

FITC-Dextran (MW 10000) is a marker consisting of coupling fluorescein-isothiocyanate to dextran (10 kDa). FITC-Dextran are polysaccharides comprised of varying lengths of branched glucose molecules and can be used to determine solute, ion and protein permeability of the blood-brain barrier (BBB) based on the size of the dextran used[1][2][3].

分子量

10000.00(Average)

CAS 号

60842-46-8

中文名称

荧光素异硫氰酸酯-葡聚糖(MW 10000)

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)

溶解性数据
In Vitro:;

H2O : 50 mg/mL (Need ultrasonic)

参考文献
  • [1]. Natarajan R, et al. Fluorescein Isothiocyanate (FITC)-Dextran Extravasation as a Measure of Blood-Brain Barrier Permeability. Curr Protoc Neurosci. 2017 Apr 10;79:9.58.1-9.58.15.

    [2]. Hoffmann A, et al. High and Low Molecular Weight Fluorescein Isothiocyanate (FITC)-Dextrans to Assess Blood-Brain Barrier Disruption: Technical Considerations. Transl Stroke Res. 2011 Mar;2(1):106-11.

    [3]. Eriksson I, et al. Analysis of Lysosomal pH by Flow Cytometry Using FITC-Dextran Loaded Cells. Methods Mol Biol. 2017;1594:179-189.

FITC-Dextran (MW 4000)(Synonyms: 荧光素异硫氰酸酯-葡聚糖(MW 4000))

FITC-Dextran (MW 4000);(Synonyms: 荧光素异硫氰酸酯-葡聚糖(MW 4000))

FITC-Dextran (MW 4000) 是由 FITC 偶联葡聚糖形成的一种标记物 (4 kDa)。FITC-Dextran 是由不同长度的支链葡萄糖分子组成的被标记的多糖,可根据所使用的葡聚糖的大小来测定血脑屏障的溶质、离子和蛋白质渗透性。

FITC-Dextran (MW 4000)amp;;(Synonyms: 荧光素异硫氰酸酯-葡聚糖(MW 4000))

FITC-Dextran (MW 4000) Chemical Structure

CAS No. : 60842-46-8

规格 价格 是否有货 数量
5 mg ¥700 In-stock
10 mg ¥1100 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

FITC-Dextran (MW 4000) 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

FITC-Dextran (MW 4000) is a marker consisting of coupling fluorescein-isothiocyanate to dextran (4 kDa). FITC-Dextran are polysaccharides comprised of varying lengths of branched glucose molecules and can be used to determine solute, ion and protein permeability of the blood-brain barrier (BBB) based on the size of the dextran used[1][2][3].

分子量

4000.00(Average)

CAS 号

60842-46-8

中文名称

荧光素异硫氰酸酯-葡聚糖(MW 4000)

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

溶解性数据
In Vitro:;

DMSO : 16.67 mg/mL (Need ultrasonic)

H2O : 16.67 mg/mL (Need ultrasonic)

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: 1.67 mg/mL (Infinity mM); Clear solution; Need ultrasonic

    此方案可获得 1.67 mg/mL (Infinity mM) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 16.7 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: 1.67 mg/mL (Infinity mM); Suspended solution; Need ultrasonic

    此方案可获得 1.67 mg/mL (Infinity mM) 的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例,取 100 μL 16.7 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂:;10% DMSO ;; 90% corn oil

    Solubility: 1.67 mg/mL (Infinity mM); Clear solution; Need ultrasonic

    此方案可获得 1.67 mg/mL (Infinity mM) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 16.7 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Natarajan R, et al. Fluorescein Isothiocyanate (FITC)-Dextran Extravasation as a Measure of Blood-Brain Barrier Permeability. Curr Protoc Neurosci. 2017 Apr 10;79:9.58.1-9.58.15.

    [2]. Hoffmann A, et al. High and Low Molecular Weight Fluorescein Isothiocyanate (FITC)-Dextrans to Assess Blood-Brain Barrier Disruption: Technical Considerations. Transl Stroke Res. 2011 Mar;2(1):106-11.

    [3]. Eriksson I, et al. Analysis of Lysosomal pH by Flow Cytometry Using FITC-Dextran Loaded Cells. Methods Mol Biol. 2017;1594:179-189.

荧光染料FITC-C6-DEVD-FMK

荧光染料Dye Reagents FITC-C6-DEVD-FMK;

FITC-C6-DEVD-FMK 是一种 Caspase-3 荧光标记抑制剂。可用于检测哺乳动物细胞凋亡中的活性 caspase-3。Z-DEVD-FMK 是一种特异性 caspase-3 抑制剂。

FITC-C6-DEVD-FMK

FITC-C6-DEVD-FMK Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

FITC-C6-DEVD-FMK, a fluorescently labeled caspase-3 inhibitor, can be used for detection of active caspase-3 in mammalian cells undergoing apoptosis. FITC-C6-DEVD-FMK provides a convenient means for sensitive detection of activated caspase-3 in living cells. Z-DEVD-FMK is a specific caspase-3 inhibitor[1].

分子量

994.99

Formula

C46H51FN6O16S

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Kanthasamy AG, et al. A novel peptide inhibitor targeted to caspase-3 cleavage site of a proapoptotic kinase protein kinase C delta (PKCdelta) protects against dopaminergic neuronal degeneration in Parkinson’s disease models. Free Radic Biol Med. 2006 Nov

荧光染料FITC-C6-LEHD-FMK

荧光染料Dye Reagents FITC-C6-LEHD-FMK;

FITC-C6-LEHD-FMK 是一种 caspase-9 荧光标记抑制剂。可用于检测哺乳动物细胞凋亡中的活性 caspase-9。Z-DEVD-FMK 是一种特异性 caspase-9 抑制剂。

FITC-C6-LEHD-FMK

FITC-C6-LEHD-FMK Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

FITC-C6-LEHD-FMK, a fluorescently labeled caspase-9 inhibitor, can be used for detection of active caspase-9 in mammalian cells undergoing apoptosis. FITC-C6-DEVD-FMK provides a convenient means for sensitive detection of activated caspase-9 in living cells. Z-LEHD-FMK is a specific caspase-9 inhibitor[1].

分子量

1031.07

Formula

C49H55FN8O14S

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Ozoren N, et, al. The caspase 9 inhibitor Z-LEHD-FMK protects human liver cells while permitting death of cancer cells exposed to tumor necrosis factor-related apoptosis-inducing ligand. Cancer Res. 2000 Nov 15; 60(22): 6259-65.

荧光染料Geldanamycin-FITC

荧光染料Dye Reagents Geldanamycin-FITC;

Geldanamycin-FITC 是一种格尔达霉素荧光探针,可用于 HSP90 抑制剂的荧光偏振分析。Geldanamycin-FITC 也可用于细胞表面 HSP90 的检测。

Geldanamycin-FITCamp;;

Geldanamycin-FITC Chemical Structure

规格 是否有货
500 μg 询价

* Please select Quantity before adding items.

生物活性

Geldanamycin-FITC, a Geldanamycin fluorescent probe, can be used in a fluorescence polarization assay for HSP90 inhibitors. Geldanamycin-FITC also can be used for detection of cell surface HSP90[1][2][3].

IC50 Target[1]

HSP90

;

体外研究
(In Vitro)

The HSP90 inhibitor Geldanamycin, when conjugated with FITC (GA–FITC) is cell-membrane impermeable, binding only cell surface HSP90 and inhibiting its functions but having no effect on cytosolic HSP90[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1034.18

Formula

C55H63N5O13S

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Kim J, et al. Development of a fluorescence polarization assay for the molecular chaperone Hsp90. J Biomol Screen. 2004;9(5):375‐381.

    [2]. Posfai D, et al. Identification of Hsp90 Inhibitors with Anti-Plasmodium Activity. Antimicrob Agents Chemother. 2018;62(4):e01799-17. Published 2018 Mar 27.

    [3]. Imai T, et al. Heat shock protein 90 (HSP90) contributes to cytosolic translocation of extracellular antigen for cross-presentation by dendritic cells. Proc Natl Acad Sci U S A. 2011;108(39):16363‐16368.

荧光染料6-FITC(Synonyms: 6-异硫氰酸荧光素; 6-Fluorescein Isothiocyanate)

荧光染料Dye Reagents 6-FITC;(Synonyms: 6-异硫氰酸荧光素; 6-Fluorescein Isothiocyanate)

6-FITC (6-Fluorescein Isothiocyanate) 是荧光素衍生物,广泛应用于流式细胞仪等。

6-FITC(Synonyms: 6-异硫氰酸荧光素; 6-Fluorescein Isothiocyanate)

6-FITC Chemical Structure

CAS No. : 3326-31-6

规格 价格 是否有货
10;mM;*;1 mL in DMSO ¥2046 询问价格 货期
100 mg ¥1860 询问价格 货期
500 mg ¥5580 询问价格 货期

* Please select Quantity before adding items.

6-FITC 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Anti-Cancer Compound Library

生物活性

6-FITC (6-Fluorescein Isothiocyanate) is a derivative of fluorescein used in wide-ranging applications including flow cytometry.

分子量

389.38

Formula

C21H11NO5S

CAS 号

3326-31-6

中文名称

6-异硫氰酸荧光素;6-异硫氰酸荧光素酯

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, protect from light, stored under nitrogen

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light, stored under nitrogen)

溶解性数据
In Vitro:;

DMSO : ≥ 35 mg/mL (89.89 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.5682 mL 12.8409 mL 25.6819 mL
5 mM 0.5136 mL 2.5682 mL 5.1364 mL
10 mM 0.2568 mL 1.2841 mL 2.5682 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。