标签归档：flag

3X FLAG peptide

发表于由

3X FLAG peptide; 纯度: 98.32%

3X FLAG peptide 是一种合成的多肽，包含三个重复的 Asp-Tyr-Lys-Xaa-Xaa-Asp 基序。

3X FLAG peptideamp;;

3X FLAG peptide Chemical Structure

CAS No. : 402750-12-3

规格 价格 是否有货 数量
1 mg ￥600 In-stock
5 mg ￥1200 In-stock
10 mg ￥2100 In-stock
25 mg ￥4200 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

3X FLAG peptide 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

3X FLAG Peptide is a synthetic peptide with a 3-time repeated DYKXXD motif.

体外研究
(In Vitro)

3X FLAG peptide can be used to label protein and other molecules.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

2861.87

Formula

C120H169N31O49S
CAS 号

402750-12-3
Sequence Shortening

MDYKDHDGDYKDHDIDYKDDDDK
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

H2O : 2 mg/mL (0.70 mM; ultrasonic and adjust pH to 3 with HCl)
参考文献

  • [1]. Zilio N, et al. Improved Tandem Affinity Purification Tag and Methods for Isolation of Proteins and Protein Complexes from Schizosaccharomyces pombe. Cold Spring Harb Protoc. 2017 Mar 1;2017(3):pdb.prot091611.

Exalpha AFLAG Chicken anti FLAG Epitope (DYKDDDDK) 0.1 mg

发表于由

上海金畔生物科技有限公司提供Exalpha AFLAG Chicken anti FLAG Epitope (DYKDDDDK) 0.1 mg，欢迎访问官网了解更多产品信息和订购

品牌 货号 产品名称 规格 价格

Exalpha AFLAG Chicken anti FLAG Epitope (DYKDDDDK) 0.1 mg 3150.00

Exalpha ADIG-F Chicken anti Dog IgG (H+L), conjugated with FITC 0.5 mg 2919.00

Exalpha ADIG Chicken anti Dog IgG (H+L) 0.5 mg 2499.00

Exalpha ACMYC-B Chicken anti C-MYC Epitope Tag, conjugated with Biotin 0.1 mg 3465.00

Exalpha ACMYC Chicken anti C-MYC Epitope Tag 0.1 mg 3150.00

Exalpha ACH Chicken anti Canine Heartworm 0.1 mg 2541.00

Exalpha ACerIG-HRP Chicken anti Cervid IgG (H+L), conjugated with HRP 0.5 mg 2856.00

Exalpha ACerIG-F Chicken anti Cervid IgG (H+L), conjugated with FITC 0.5 mg 2856.00

Exalpha ACerIG Chicken anti Cervid IgG (H+L) 0.5 mg 2499.00

Exalpha ACdTB-HRP Chicken anti Clostridium difficile Toxin B, conjugated with HRP 0.1 mg 5040.00

Exalpha ACdTB-F Chicken anti Clostridium difficile Toxin B, conjugated with FITC 0.1 mg 5040.00

Exalpha ACdTB-B Chicken anti Clostridium difficile Toxin B, conjugated with Biotin 0.1 mg 5040.00

Exalpha ACdTB Chicken anti Clostridium difficile Toxin B 0.1 mg 4515.00

Exalpha ACdTA-HRP Chicken anti Clostridium difficile Toxin A, conjugated with HRP 0.1 mg 4599.00

Exalpha ACdTA-F Chicken anti Clostridium difficile Toxin A, conjugated with FITC 0.1 mg 4599.00

Exalpha ACdTA Chicken anti Clostridium difficile Toxin A 0.1 mg 3969.00

Exalpha ACdGDH-F Chicken anti Clostridium difficile Glutamate Dehydrogenase (GDH), conjugated with FITC 0.1 mg 3465.00

Exalpha ACdGDH Chicken anti Clostridium difficile Glutamate Dehydrogenase (GDH) 0.1 mg 2835.00

Exalpha ACdBTSB-F Chicken anti Clostridium difficile Binary Toxin, Subunit B, conjugated with FITC 0.1 mg 5691.00

Exalpha ACdBTSB Chicken anti Clostridium difficile Binary Toxin, Subunit B 0.1 mg 5523.00

Flag 品牌：Anygen

发表于由

Flag

品牌：Anygen
CAS No.：
储存条件：-20℃
纯度：–
产品编号

(生产商编号)

 等级 规格 运输包装 零售价(RMB) 库存情况 参考值

304-92301

 – 0.5 mg – 咨询 – –


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。

Flag抗体—抗Flag标签抗体

发表于由

融合标签，如Flag、GST等标签的使用可以简化蛋白质的纯化过程、控制蛋白质固定的空间取向及方便检测、 使
体内生物事件可视化、提高重组蛋白质的产量、增强重组蛋白质的可溶性和稳定性等。常用的标签包括myc、HA、Flag、His、GST等。其中Flag标签系统利用一个短的亲水性八氨基酸肽（DYKDDDDK）融合到目标蛋白。

Flag抗体—抗Flag标签抗体
检测Bad-Flag融合蛋白

Flag标签可位于蛋白质的C端或N端，该系统已广泛应用于各种细胞类型，包括细菌、酵母和哺乳动物细胞等，相应的Flag标签抗体也被广泛应用。由于Flag标签系统的纯化条件是非变性的，因此可以纯化所有有活性的融合蛋白。Flag标签可以通过加入肠激酶处理去除，肠激酶专一识别该肽序列C末端的5个氨基酸残基。

Flag抗体可以用于检测和Flag标签融合表达蛋白的表达、细胞内定位，以及纯化、定性或定量检测Flag融合表达蛋白等。上海金畔生物科技有限公司致力于为客户提供最具性价比的免疫学试剂和解决方案，如各种标签抗体、内参抗体、常规一抗以及全系列二抗，抗体检测底物以及相关试剂盒等。针对Flag标签抗体，则有多种不同样式产品供您选择。

上海金畔生物科技有限公司提供的Flag标签抗体均为原装进口包装，且备有现货。上海金畔生物科技有限公司提供的所有优质标签抗体产品，条带清晰，高效敏感，能为您提供最值得信赖的标签抗体产品保障和售后服务。

货号 产品名称 宿主 应用 价格/规格
CB100145M Anti-Flag(Monoclonal) Ms WB、IP、IHC ￥1600/100μg
  产品说明：美国Cali-Bio原装进口抗体，小鼠源单抗，Sigma的Anti-FLAG® M2一样，可识别位于融合蛋白C和N末端的Flag标签。100μg/150μl，可用于WB/IP/IHC等检测。更多该产品详情请点击：Anti-Flag抗体说明书。
CB100205M Anti-Flag(Monoclonal) Ms ELISA ￥2514/500μg|￥4686/1mg
  产品说明：美国Cali-Bio原装进口抗体，小鼠源单抗，分为500μg和1mg两种包装，非常适合做ELISA检测，
T503 Anti-Flag Rb WB ￥1000/50μg|￥1500/100μg
  产品说明：美国SignalWay Antibody原装进口抗体，兔源多抗，Sigma的Anti-FLAG® M2一样，可识别位于融合蛋白C和N末端的Flag标签。可用于WB，WB推荐稀释度为1:1000。分50μg和100μg两种包装。更多该产品详情请点击：Anti-Flag抗体说明书。
更多Flag抗体请来电查询，价格可能有调整，及时报价请使用货号在本站右侧产品查询栏内查询。

作为多家国际知名抗体公司的中国一级及一级代理商，上海金畔生物科技有限公司致力于为客户提供最具性价比的常规免疫学试剂，如各种标签抗体、内参抗体、常规一抗以及全系列二抗，抗体检测底物以及相关试剂盒等。针对标签蛋白的检测及标签抗体，除了上述Flag抗体外，还有其它常规标签抗体，如GST抗体、GFP抗体、HA抗体、His抗体、Myc抗体等标签检测抗体，均备有现货。

另外，上海金畔生物科技有限公司还提供全系列原装进口二抗现货备货，如果您对上述产品感兴趣，请致电021-50837765到上海金畔生物科技有限公司垂询相关的产品信息及完整实验解决方案。

FLAG peptide

发表于由

FLAG peptide; 纯度: 99.21%

FLAG peptide是一种八个氨基酸组成的多肽 (Asp-Ty-rLys-Asp-Asp-Asp-Asp-Lys)，具有肠激酶切割位点。FLAG peptide用于抗体介导的重组蛋白的识别和纯化。

FLAG peptideamp;;

FLAG peptide Chemical Structure

CAS No. : 98849-88-8

规格 价格 是否有货 数量
1 mg ￥500 In-stock
5 mg ￥900 In-stock
10 mg ￥1300 In-stock
25 mg ￥2390 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

FLAG peptide 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptidomimetic Library

生物活性

FLAG peptide is an eight amino acids peptide (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) with an enterokinase-cleavage site; designed for antibody-mediated identification and purification of recombinant proteins.

体外研究
(In Vitro)

Fusion protein technology has become an important tool for solving numerous problems linked to recombinant protein production. The properties of the additional tag facilitate identification and provide a one-step purification procedure of the fusion protein by passing cell extracts or supernatants through columns of an appropriate matrix. FLAG peptide allows elution under non-denaturing conditions. Several antibodies against FLAG peptide have been developed. One antibody, M1, binds the peptide in the presence of bivalent metal cations, preferably Ca2+. Elution is effected by chelating agents. Another strategy is competitive elution with excess of free FLAGe peptide. Antibodies M2 and M5 are applied in this procedure[1]. The Flag-tag is first described as a calcium-dependent epitope of a monoclonal antibody. It is a highly acidic octapeptide which can be N-terminally fused to the protein of interest. As a very hydrophilic peptide the Flag–tag has a high surface probability. Flag-fusion proteins can be captured by an immunoaffinity column in the presence of Ca2+ and eluted byEDTA at low concentrations, neutral pH and thus, nearly physiological conditions[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1012.97

Formula

C41H60N10O20
CAS 号

98849-88-8
Sequence

Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys
Sequence Shortening

DYKDDDDK
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Protect from light, stored under nitrogen

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light, stored under nitrogen)
溶解性数据
In Vitro:;

H2O : 100 mg/mL (98.72 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.9872 mL 4.9360 mL 9.8720 mL
5 mM 0.1974 mL 0.9872 mL 1.9744 mL
10 mM 0.0987 mL 0.4936 mL 0.9872 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Einhauer A, et al. The FLAG peptide, a versatile fusion tag for the purification of recombinant proteins. J Biochem Biophys Methods. 2001 Oct 30;49(1-3):455-65.

    [2]. Schuster M, et al. Protein expression in yeast; comparison of two expression strategies regarding proteinmaturation. J Biotechnol. 2000 Dec 28;84(3):237-48.

3X FLAG peptide TFA

发表于由

3X FLAG peptide TFA; 纯度: 99.79%

3X FLAG peptide TFA 是一种合成的多肽，包含三个重复的 Asp-Tyr-Lys-Xaa-Xaa-Asp 基序。

3X FLAG peptide TFAamp;;

3X FLAG peptide TFA Chemical Structure

规格 价格 是否有货 数量
1 mg ￥600 In-stock
5 mg ￥1200 In-stock
10 mg ￥2100 In-stock
25 mg ￥4200 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

3X FLAG peptide TFA is a synthetic peptide with a 3-time repeated DYKXXD motif[1].

体外研究
(In Vitro)

3X FLAG peptide can be used to label protein and other molecules.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

2975.84

Formula

C122H170F3N31O51S
Sequence

Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys
Sequence Shortening

MDYKDHDGDYKDHDIDYKDDDDK
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)
溶解性数据
In Vitro:;

H2O : 25 mg/mL (8.40 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.3360 mL 1.6802 mL 3.3604 mL
5 mM 0.0672 mL 0.3360 mL 0.6721 mL
10 mM — — —

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;PBS

    Solubility: 50 mg/mL (16.80 mM); Clear solution; Need ultrasonic and adjust pH to 3 with HCl

  • 2.

    请依序添加每种溶剂：;20 mM Tris pH7.5, 100 mM NaCl

    Solubility: 33.33 mg/mL (11.20 mM); Clear solution; Need ultrasonic

*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Zilio N, et al. Improved Tandem Affinity Purification Tag and Methods for Isolation of Proteins and Protein Complexes from Schizosaccharomyces pombe. Cold Spring Harb Protoc. 2017 Mar 1;2017(3):pdb.prot091611.