N-(Azido-PEG3)-N-Fluorescein-PEG3-acid Chemical Structure
CAS No. : 2100306-50-9
规格
是否有货
1 mg
询价
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生物活性
N-(Azido-PEG3)-N-Fluorescein-PEG3-acid is a PEG-based PROTAC linker which contains azide, fluorescein and carboxylic acid moieties.
IC50 Target
PEGs
;
体外研究 (In Vitro)
PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
811.85
Formula
C38H45N5O13S
CAS 号
2100306-50-9
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
FITC (Fluorescein 5-isothiocyanate) is a fluorescence probe for the labeling of amines. FITC is a pH- and Cu2+-sensitive fluorescence dye[1][2].
体外研究 (In Vitro)
Annexin V-FITC/PI is employed to detect chondrocyte apoptosis[1]. The PEI-modified CDs are conjugated with fluorescein isothiocyanate (FITC) to fabricate CDs-FITC composites[2].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
389.38
Formula
C21H11NO5S
CAS 号
3326-32-7
中文名称
异硫氰酸荧光素酯；荧光素-5-异氰酸酯
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
-20deg;C, protect from light, stored under nitrogen
*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light, stored under nitrogen)
溶解性数据
In Vitro:;
DMSO : 83.33 mg/mL (214.01 mM; Need ultrasonic)
H2O : < 0.1 mg/mL (insoluble)
配制储备液
浓度溶剂体积质量
1 mg
5 mg
10 mg
1 mM
2.5682 mL
12.8409 mL
25.6819 mL
5 mM
0.5136 mL
2.5682 mL
5.1364 mL
10 mM
0.2568 mL
1.2841 mL
2.5682 mL
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献
[1]. Gao SG, et al. Phosphorylation of osteopontin has proapoptotic and proinflammatory effects on human knee osteoarthritis chondrocytes. Exp Ther Med. 2016 Nov;12(5):3488-3494. Epub 2016 Oct 5.
[2]. Zhu X, et al. Ratiometric, visual, dual-signal fluorescent sensing and imaging of pH/copper ions in real samples based on carbon dots-fluorescein isothiocyanate composites. Talanta. 2017 Jan 1;162:65-71
Cell Assay [1]
The frequency of chondrocyte apoptosis is measured by flow cytometry with Annexin V-fluorescein isothiocyanate (FITC) and propidium iodide. A total of 1×104 treated chondrocytes are collected from each group, washed in cold PBS and incubated with Annexin V-FITC and PI at room temperature for 15 min in the dark on ice. These samples are then analyzed using a fluorescence-activated cell sorter. Cell Quest software is used to analyze the percentage of apoptosis. All tests are repeated in triplicate.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献
[1]. Gao SG, et al. Phosphorylation of osteopontin has proapoptotic and proinflammatory effects on human knee osteoarthritis chondrocytes. Exp Ther Med. 2016 Nov;12(5):3488-3494. Epub 2016 Oct 5.
[2]. Zhu X, et al. Ratiometric, visual, dual-signal fluorescent sensing and imaging of pH/copper ions in real samples based on carbon dots-fluorescein isothiocyanate composites. Talanta. 2017 Jan 1;162:65-71
5MP-Fluorescein (compound 3e) is a fluorescein dye base on 5-Methylene pyrrolone (5MP).5-Methylene pyrrolones are highly thiol-specific and tracelessly removable bioconjugation tools[1].
分子量
425.39
Formula
C25H15NO6
CAS 号
2100282-58-2
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献
[1]. Yingqian Zhang, et al. Thiol Specific and Tracelessly Removable Bioconjugation via Michael Addition to 5-Methylene Pyrrolones. J Am Chem Soc. 2017 May 3;139(17):6146-6151.
Fluorescein diacetate 是一种细胞渗透性的酯酶底物。Fluorescein diacetate 可作为人谷胱甘肽硫转移酶 Pi (hGSTP1-1) 的荧光底物。
Fluorescein Diacetate Chemical Structure
CAS No. : 596-09-8
规格
价格
是否有货
数量
Free Sample (0.1-0.5 mg)
;
Apply now
;
10;mM;*;1 mL in DMSO
￥500
In-stock
500 mg
￥400
In-stock
5 g
￥500
In-stock
10 g
;
询价
;
50 g
;
询价
;
* Please select Quantity before adding items.
Fluorescein Diacetate 相关产品
bull;相关化合物库:
Bioactive Compound Library Plus
生物活性
Fluorescein diacetate is a cell permeable esterase-substrate. Fluorescein diacetate can be used as a fluorogenic substrate for hGSTP1-1.
体外研究 (In Vitro)
Fluorescein diacetate (FDA) is an acetylated derivative of the green fluorescent dye fluorescein[1]. Fluorescein diacetate (FDA), a fluorescent probe used for vital staining, is a fluorescently activated by esterolytic activity of human Pi-class glutathione S-transferase (hGSTP1) selectively among various cytosolic GSTs. Fluorescence activation of Fluorescein diacetate susceptible to GST inhibitors is observed in MCF7 cells exogenously overexpressing hGSTP1, but not in cells overexpressing hGSTA1 or hGSTM1. Fluorescein diacetate can be used as a fluorogenic substrate for hGSTP1-1. To investigate whether the fluorescence activation is due to hGSTP1 activity, Fluorescein diacetate is incubated with recombinant hGSTP1-1 and GSH in vitro. Remarkable fluorescence activation is observed in the presence of both hGSTP1-1 and GSH, whereas only slight activation is observed in the absence of either of them or when the enzyme is heat inactivated. This suggests that the fluorescence activation of Fluorescein diacetate depends on hGSTP1-1 activity. From the linear relationship between the rate of increase in fluorescence and the hGSTP1-1 concentration, the specific activity of the enzyme for 1 μM Fluorescein diacetate is determined to be 79±15 nmol/min/mg protein. Fluorescein diacetate is applicable as a fluorogenic substrate for evaluating inhibitors of GSTP1-1 in vitro. For Fluorescein diacetate as a substrate, both Ethacrynic acid (EA) and NBDHEX suppress the hGSTP1-1-dependent fluorescent increase in a concentration-dependent manner, with IC50s of 3.3±0.5 μM and 0.61±0.04 μM, respectively[2].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
416.38
Formula
C24H16O7
CAS 号
596-09-8
中文名称
二乙酸荧光素；荧光素二乙酸盐；荧光素二乙酸酯
运输条件
Room temperature in continental US; may vary elsewhere.
[1]. Boyd V, et al. Limitations in the Use of Fluorescein Diacetate/Propidium Iodide (FDA/PI) and Cell PermeableNucleic Acid Stains for Viability Measurements of Isolated Islets of Langerhans. Curr Trends Biotechnol Pharm. 2008 Mar;2(2):66-84.
[2]. Fujikawa Y, et al. Fluorescein diacetate (FDA) and its analogue as substrates for Pi-class glutathione S-transferase (GSTP1) and their biological application. Talanta. 2018 Mar 1;179:845-852.
Cell Assay [2]
MCF7 cells (2-3×105) are seeded in a 35-mm glass bottom dish before the experiment. Prior to imaging, cells are washed with 1 mL PBS, then incubated in 1 mL Hanks’ Balanced Salt solution (HBSS(+) without phenol red) containing 1 μM Fluorescein diacetate (0.1% DMSO as a cosolvent) for 5 min at 37°C. Cells are washed twice with 1 mL PBS and 1 mL HBSS is added before imaging. Fluorescence images of fluorescein and DsRed-Express2 were acquired in the FITC channel (excitation at 473 nm) and the DsRed channel (excitation 559 nm). The 16-bit images obtained are analysed[2].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献
[1]. Boyd V, et al. Limitations in the Use of Fluorescein Diacetate/Propidium Iodide (FDA/PI) and Cell PermeableNucleic Acid Stains for Viability Measurements of Isolated Islets of Langerhans. Curr Trends Biotechnol Pharm. 2008 Mar;2(2):66-84.
[2]. Fujikawa Y, et al. Fluorescein diacetate (FDA) and its analogue as substrates for Pi-class glutathione S-transferase (GSTP1) and their biological application. Talanta. 2018 Mar 1;179:845-852.
Br-5MP-Fluorescein is a dye reagent for labeling of peptides and proteins[1].
分子量
504.29
Formula
C25H14BrNO6
CAS 号
2468100-39-0
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献
[1]. Yingqian Zhang, et al. Cysteine-specific protein multi-functionalization and disulfide bridging using 3-bromo-5-methylene pyrrolones. Nat Commun. 2020 Feb 21;11(1):1015.
Fluorescein (Uranine) is widely used as a fluorescent tracer in medicinal and biological applications and tumor infected tissues tracer. Fluorescein (Uranine) is a representative green fluorophore that has been widely used as a scaffold of practically useful green fluorescent probes[1][2].
体外研究 (In Vitro)
Fluorescein is a synthetic organic photoactive dye compound soluble in water, alcohol and polar solvents[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
分子量
332.31
Formula
C20H12O5
CAS 号
2321-07-5
中文名称
荧光素；荧光黄；荧光红；荧光橙；酸性黄73
运输条件
Room temperature in continental US; may vary elsewhere.
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献
[1]. Nabel A Negm, et al. Fluorescein dye derivatives and their nanohybrids: Synthesis, characterization and antimicrobial activity. J Photochem Photobiol B. 2016 Sep;162:421-433.
[2]. Li Liu, et al. Fluorescein as an artificial enzyme to mimic peroxidase. Chem Commun (Camb). 2016 Nov24;52(96):13912-13915.
[3]. RobertSjöback, et al. Absorption and fluorescence properties of fluorescein. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
Fluorescein di(β-D-galactopyranoside) Chemical Structure
CAS No. : 17817-20-8
规格
价格
是否有货
1 mg
￥1500
询问价格 货期
* Please select Quantity before adding items.
生物活性
Fluorescein di(β-D-galactopyranoside) is a fluorogenic substrate for β-galactosidase (λex=485 nm, λem=535 nm).
体外研究 (In Vitro)
The fluorescence produced by Fluorescein di(β-D-galactopyranoside) increases in a time- and dose-dependent manner. The level of fluorescence produced by the double-substrate method is much lower than that by the Fluorescein di(β-D-galactopyranoside) method. Results show that the fluorescein produced by Fluorescein di(β-D-galactopyranoside) in Hs68 cells is proportional to the number of passages[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
656.59
Formula
C32H32O15
CAS 号
17817-20-8
中文名称
荧光素二(β-D-吡喃半乳糖苷)
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
-20deg;C, protect from light
*该产品在溶液状态不稳定，建议您现用现配，即刻使用。
参考文献
[1]. Yang NC, et al. A fluorimetric method using fluorescein di-beta-D-galactopyranoside for quantifying the senescence-associated beta-galactosidase activity in human foreskin fibroblast Hs68 cells. Anal Biochem. 2004 Feb 15;325(2):337-43.
Cell Assay [1]
The cells (5×103 cells per well) are cultured in a 96-well plate overnight for attachment, washed, and then fixed in solutions. An aliquot (100 μL) of the reaction buffer (i.e., the staining solution without X-Gal) is added into each well. Then, 10 μL of 2 mM Fluorescein di(β-D-galactopyranoside) is added per well and the plate is incubated in the dark at 37°C for 24 h without CO2 supply. After incubation at 37°C for 24 h, 100 μL of the supernatant is transferred to a 96-well plate for fluorescent measurement in triplicates. The fluorescein fluorescence is measured using a fluorometer with an excitation at 485 nm and an emission at 535 nm[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献
[1]. Yang NC, et al. A fluorimetric method using fluorescein di-beta-D-galactopyranoside for quantifying the senescence-associated beta-galactosidase activity in human foreskin fibroblast Hs68 cells. Anal Biochem. 2004 Feb 15;325(2):337-43.
Hydroxyphenyl fluorescein (HPF) is the reagent that can directly detect highly reactive oxygen species (hROS). Hydroxyphenyl fluorescein selectively and dose-dependently reacts with hROS, such as the hydroxyl radical and peroxynitrite, which exhibit strong fluorescence[1][2].
体外研究 (In Vitro)
The fluorescence of Hydroxyphenyl Fluorescein (HPF) is greatly enhanced following incubation with blue light illuminated blebbistatin (30 min). Fluorescence enhancement of Hydroxyphenyl Fluorescein occurred equally efficiently under Ar purged and non-Ar purged conditions, suggesting that singlet oxygen as an intermediate involved is improbable. Fluorescence measurement with Hydroxyphenyl Fluorescein suggests a negative linear relationship between fluorescence intensity of Hydroxyphenyl Fluorescein (10 μM) and thiourea concentration (0-1 mM)[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究 (In Vivo)
The Hydroxyphenyl Fluorescein (HPF) probe (15 μl, 25 μM) is given intratesticularly in anaesthetized mice 20 min before IR (ionizing radiation). The accumulation of •OH by the fluorescence signal emitted by the oxidized form of HPF is assessed[3].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
424.40
Formula
C26H16O6
CAS 号
359010-69-8
中文名称
羟苯基荧光素
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献
[1]. Ming-De Li, et al. Dynamics of Oxygen-Independent Photocleavage of Blebbistatin as a One-Photon Blue or Two-Photon Near-Infrared Light-Gated Hydroxyl Radical Photocage. J Am Chem Soc. 2018 Nov 21;140(46):15957-15968.
[2]. Yoichiro Masuda, et al. Free radical production by femtosecond laser lens irradiation in porcine eyes. J Cataract Refract Surg. 2019 Aug;45(8):1168-1171.
[3]. Yunhai Chuai, et al. Hydrogen-rich saline attenuates radiation-induced male germ cell loss in mice through reducing hydroxyl radicals. Biochem J. 2012 Feb 15;442(1):49-56.
