标签归档：fret

Cathepsin D and E FRET Substrate acetate

发表于由

Cathepsin D and E FRET Substrate acetate; 纯度: 99.06%

Cathepsin D and E FRET Substrate acetate 为 cathepins D 和 E 的荧光底物 (fluorogenic substrate)，而不是 B、H 或 I 的荧光底物，它的裂解发生在 Phe-Phe 酰胺键处。Cathepsin D and E FRET Substrate 是用于组织蛋白酶 D 和组织蛋白酶 E 的常规检测和机制研究的有用工具。(激发波长:328 nm; 发射波长:393 nm)

Cathepsin D and E FRET Substrate acetateamp;;

Cathepsin D and E FRET Substrate acetate Chemical Structure

规格 价格 是否有货 数量
5 mg ￥2800 In-stock
10 mg ￥4500 In-stock
25 mg ￥8900 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Cathepsin D and E FRET Substrate acetate 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Cathepsin D and E FRET Substrate acetate is a fluorogenic substrate for cathepsins D and E and not for B, H or L. The cleavage occurs at the Phe-Phe amide bond resul. Cathepsin D and E FRET Substrate is a valuable tool for routine assays and for mechanistic studies on cathepsins E and D[1].

分子量

1816.15

Formula

C87H126N22O21
Sequence

MOCAc-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-{Lys(Dnp)}-{D-Arg}-NH2
Sequence Shortening

MOCAc-GKPILFFRL-{Lys(Dnp)}-{D-Arg}-NH2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)
溶解性数据
In Vitro:;

H2O : 10 mg/mL (5.51 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.5506 mL 2.7531 mL 5.5062 mL
5 mM 0.1101 mL 0.5506 mL 1.1012 mL
10 mM — — —

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Y Yasuda, et al. Characterization of new fluorogenic substrates for the rapid and sensitive assay of cathepsin E and cathepsin D. J Biochem

Cathepsin D and E FRET Substrate

发表于由

Cathepsin D and E FRET Substrate;

Cathepsin D and E FRET Substrate 为 cathepins D 和 E 的荧光底物 (fluorogenic substrate)，而不是 B、H 或 I 的荧光底物，它的裂解发生在 Phe-Phe 酰胺键处。Cathepsin D and E FRET Substrate 是用于组织蛋白酶 D 和组织蛋白酶 E 的常规检测和机制研究的有用工具。(激发波长:328 nm; 发射波长:393 nm)

Cathepsin D and E FRET Substrateamp;;

Cathepsin D and E FRET Substrate Chemical Structure

CAS No. : 839730-93-7

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Cathepsin D and E FRET Substrate 的其他形式现货产品:

Cathepsin D and E FRET Substrate acetate

生物活性

Cathepsin D and E FRET Substrate is a fluorogenic substrate for cathepsins D and E and not for B, H or L. The cleavage occurs at the Phe-Phe amide bond resul. Cathepsin D and E FRET Substrate is a valuable tool for routine assays and for mechanistic studies on cathepsins E and D[1].

分子量

1756.10

Formula

C85H122N22O19
CAS 号

839730-93-7
Sequence

MOCAc-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-{Lys(Dnp)}-{D-Arg}-NH2
Sequence Shortening

MOCAc-GKPILFFRL-{Lys(Dnp)}-{D-Arg}-NH2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Y Yasuda, et al. Characterization of new fluorogenic substrates for the rapid and sensitive assay of cathepsin E and cathepsin D. J Biochem

Renin FRET Substrate I

发表于由

Renin FRET Substrate I;

Renin FRET Substrate I 是人肾素的底物。Renin FRET Substrate I 可以作为整合人血管紧张素原 N 端肽段中出现的肾素裂解位点。

Renin FRET Substrate Iamp;;

Renin FRET Substrate I Chemical Structure

CAS No. : 142988-22-5

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

Renin FRET Substrate I is a substrate of human renin. Renin FRET Substrate I is designed to incorporate the renin cleavage site that occurs in the N-terminal peptide of human angiotensinogen[1].

体外研究
(In Vitro)

Cleavage of Renin FRET Substrate I occurs specifically at the Leu-Val bond and corresponds to the renin cleavage site of angiotensinogen[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1798.20

Formula

C96H126N22O16S
CAS 号

142988-22-5
Sequence

DABCYL-gamma;-{Abu}-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-Glu-Asp-Ala-Asn-Ser
Sequence Shortening

DABCYL-gamma;-{Abu}-IHPFHLVIHT-EDANS
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. G T Wang, et al. A continuous fluorescence assay of renin activity. Anal Biochem. 1993 May 1;210(2):351-9.