Galanin (1-19), human

Galanin (1-19), human;

Galanin (1-19), human 是人类甘丙肽的 1-19 片段。Galanin (GAL) 是一种广泛分布的神经肽,具有多种生物学效应,包括调节激素释放,抗伤害作用和改善摄食行为。

Galanin (1-19), humanamp;;

Galanin (1-19), human Chemical Structure

CAS No. : 136005-51-1

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生物活性

Galanin (1-19), human is the 1-19 fragment of the human galanin. Galanin (GAL) is a widely distributed neuropeptide with diverse biological effects including modulation of hormone release, antinociception and modification of feeding behavior[1].

分子量

1964.14

Formula

C89H130N26O25

CAS 号

136005-51-1

Sequence

Gly-Trp-Thr-Leu-Asn-Ser-Ala-Gly-Tyr-Leu-Leu-Gly-Pro-His-Ala-Val-Gly-Asn-His

Sequence Shortening

GWTLNSAGYLLGPHAVGNH

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献
  • [1]. Howard AD, et al. Molecular cloning and characterization of a new receptor for galanin. FEBS Lett. 1997 Apr 1;405(3):285-90.

Galanin (swine) (TFA)

Galanin (swine) (TFA);

Galanin (swine) TFA 是一种神经肽,由 29 个氨基酸组成,含有一个 C 端酰胺化甘氨酸。在各种实验条件下,Galanin (swine) TFA 在体内和体外抑制基础和刺激的胰岛素分泌。Galanin (swine) TFA 是一种甘丙肽受体激动剂,在人 GAL1、大鼠 GAL1、人 GAL2、大鼠 GAL2、 GAL3 和大鼠 GAL3 的 pKi 分别为 9.63、9.49、9.02、8.98、8.01 和 8.14。

Galanin (swine) (TFA)amp;;

Galanin (swine) (TFA) Chemical Structure

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生物活性

Galanin (swine) TFA, a neuropeptide, consists of 29 amino acids and contains a C-terminal amidated glycine. Galanin (swine) inhibits basal and stimulated insulin secretion both in vivo and in vitro under a variety of experimental conditions. Galanin (swine) TFA is a galanin receptor agonist with pKis of 9.63, 9.49, 9.02, 8.98, 8.01 and 8.14 at human GAL1, rat GAL1, human GAL2, rat GAL2, human GAL3 and rat GAL3 respectively[1].

分子量

3324.54

Formula

C148H214F3N43O42

Sequence

Gly-Trp-Thr-Leu-Asn-Ser-Ala-Gly-Tyr-Leu-Leu-Gly-Pro-His-Ala-Ile-Asp-Asn-His-Arg-Ser-Phe-His-Asp-Lys-Tyr-Gly-Leu-Ala-NH2

Sequence Shortening

GWTLNSAGYLLGPHAIDNHRSFHDKYGLA-NH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Branchek TA, et al. Galanin receptor subtypes. Trends Pharmacol Sci. 2000;21(3):109-117.

    [2]. Ahrén B, et al. Galanin and the endocrine pancreas. FEBS Lett. 1988;229(2):233-237.

Galanin (1-29)(rat, mouse) TFA

Galanin (1-29)(rat, mouse) TFA;

Galanin (1-29)(rat, mouse) TFA 是一种非选择性甘丙肽受体 (galanin receptor) 激动剂,对 GAL1、GAL2 和 GAL3 的 Ki 值分别为 0.98、1.48 和 1.47 nM。具有抗惊厥作用。

Galanin (1-29)(rat, mouse) TFAamp;;

Galanin (1-29)(rat, mouse) TFA Chemical Structure

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100 mg ; 询价 ;
250 mg ; 询价 ;
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生物活性

Galanin (1-29)(rat, mouse) TFA is a non-selective galanin receptor agonist, with Kis of 0.98, 1.48 and 1.47 nM for GAL1, GAL2 and GAL3, respectively. Anticonvulsant effect[1][2].

分子量

3278.47

Formula

C143H212F3N43O43

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Wang S, et al. Cloning and expressional characterization of a novel galanin receptor. Identification of different pharmacophores within galanin for the three galanin receptor subtypes. J Biol Chem. 1997;272(51):31949-31952.

    [2]. Mazarati A, et al. Regulation of kindling epileptogenesis by hippocampal galanin type 1 and type 2 receptors: The effects of subtype-selective agonists and the role of G-protein-mediated signaling. J Pharmacol Exp Ther. 2006;318(2):700-708.

Galanin Receptor Ligand M35 TFA

Galanin Receptor Ligand M35 TFA;

Galanin Receptor Ligand M35 TFA 是一种高亲和力的甘丙肽受体 (galanin receptor) 配体 (Kd=0.1 nM),也是 galanin receptor 拮抗剂。Galanin Receptor Ligand M35 TFA 对人甘丙肽受体 1 型 和 人甘丙肽受体 2 型 的 Ki 值分别为 0.11 nM、2.0 nM。

Galanin Receptor Ligand M35 TFAamp;;

Galanin Receptor Ligand M35 TFA Chemical Structure

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250 mg ; 询价 ;
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Galanin Receptor Ligand M35 TFA 的其他形式现货产品:

Galanin Receptor Ligand M35

生物活性

Galanin Receptor Ligand M35 TFA is a high-affinity ligand and antagonist of galanin receptor (Kd=0.1 nM). Galanin Receptor Ligand M35 TFA exerts a Ki values of 0.11 and 2.0 nM for human galanin receptor type 1 and 2, respectively[1][2].

体外研究
(In Vitro)

Galanin Receptor Ligand M35 TFA has a dual effect on the galanin mediated inhibition of Forskolin stimulated cyclic AMP production in Rin m 5F cells. Co-applied with galanin (10 nM), Galanin Receptor Ligand M35 TFA at low concentrations (1 nM) is able to reverse the inhibitory effect of galanin. But when present at higher concentrations (15 and 30 nM), Galanin Receptor Ligand M35 TFA acts as a galanin receptor agonist, inhibiting the forskolin stimulated production of cAMP[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Galanin Receptor Ligand M35 TFA (6 nM/10 μL; i.c.v.) improves the ability of the male Sprague-Dnwley rats to acquire the swim maze task[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

2347.62

Formula

C109H154N27F3O28

Sequence

Gly-Trp-Thr-Leu-Asn-Ser-Ala-Gly-Tyr-Leu-Leu-Gly-Pro-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg-NH2

Sequence Shortening

GWTLNSAGYLLGPPPGFSPFR-NH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Kask K, et al. Binding and agonist/antagonist actions of M35, galanin(1-13)-bradykinin(2-9)amide chimeric peptide, in Rin m 5F insulinoma cells. Regul Pept. 1995 Nov 10;59(3):341-8.

    [2]. Webling KE, et al. Galanin receptors and ligands. Front Endocrinol (Lausanne). 2012 Dec 7;3:146.

    [3]. Ogren SO, et al. Evidence for a role of the neuropeptide galanin in spatial learning. Neuroscience. 1992 Nov;51(1):1-5.

Galanin Receptor Ligand M35

Galanin Receptor Ligand M35; 纯度: 99.65%

Galanin Receptor Ligand M35 是一种高亲和力的甘丙肽受体 (galanin receptor) 配体 (Kd=0.1 nM),也是 galanin receptor 拮抗剂。Galanin Receptor Ligand M35 对人甘丙肽受体 1 型 和 人甘丙肽受体 2 型 的 Ki 值分别为 0.11 nM、2.0 nM。

Galanin Receptor Ligand M35amp;;

Galanin Receptor Ligand M35 Chemical Structure

CAS No. : 142846-71-7

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1 mg ¥2000 In-stock
5 mg ¥6000 In-stock
10 mg ¥10000 In-stock
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100 mg ; 询价 ;

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Galanin Receptor Ligand M35 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
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  • Peptide Library

生物活性

Galanin Receptor Ligand M35 is a high-affinity ligand and antagonist of galanin receptor (Kd=0.1 nM). Galanin Receptor Ligand M35 exerts a Ki values of 0.11 and 2.0 nM for human galanin receptor type 1 and 2, respectively[1][2].

IC50 Target

Kd: 0.1 nM (galanin receptor)[1]
Ki: 0.11 nM (human galanin receptor type 1), 2.0 nM (human galanin receptor type 2)[2]

体外研究
(In Vitro)

Galanin Receptor Ligand M35 has a dual effect on the galanin mediated inhibition of Forskolin stimulated cyclic AMP production in Rin m 5F cells. Co-applied with galanin (10 nM), Galanin Receptor Ligand M35 at low concentrations (1 nM) is able to reverse the inhibitory effect of galanin. But when present at higher concentrations (15 and 30 nM), Galanin Receptor Ligand M35 acts as a galanin receptor agonist, inhibiting the forskolin stimulated production of cAMP[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Galanin Receptor Ligand M35 (6 nM/10 μL; i.c.v.) improves the ability of the male Sprague-Dnwley rats to acquire the swim maze task[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

2233.60

Formula

C107H153N27O26

CAS 号

142846-71-7

Sequence

Gly-Trp-Thr-Leu-Asn-Ser-Ala-Gly-Tyr-Leu-Leu-Gly-Pro-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg-NH2

Sequence Shortening

GWTLNSAGYLLGPPPGFSPFR-NH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

DMSO : 100 mg/mL (44.77 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.4477 mL 2.2385 mL 4.4771 mL
5 mM 0.0895 mL 0.4477 mL 0.8954 mL
10 mM 0.0448 mL 0.2239 mL 0.4477 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (1.12 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (1.12 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (1.12 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (1.12 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂:;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.5 mg/mL (1.12 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (1.12 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Kask K, et al. Binding and agonist/antagonist actions of M35, galanin(1-13)-bradykinin(2-9)amide chimeric peptide, in Rin m 5F insulinoma cells. Regul Pept. 1995 Nov 10;59(3):341-8.

    [2]. Webling KE, et al. Galanin receptors and ligands. Front Endocrinol (Lausanne). 2012 Dec 7;3:146.

    [3]. Ogren SO, et al. Evidence for a role of the neuropeptide galanin in spatial learning. Neuroscience. 1992 Nov;51(1):1-5.