Gp100 (619-627) (acetate)

Gp100 (619-627) (acetate);

Gp100 (619-627) acetate 是人黑素瘤抗原糖蛋白 100 (gp100) 的氨基酸 619 至 627 片段。Gp100 已被广泛用于黑素瘤免疫疗法的靶标研究。

Gp100 (619-627) (acetate)amp;;

Gp100 (619-627) (acetate) Chemical Structure

规格 是否有货
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250 mg ; 询价 ;
500 mg ; 询价 ;

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生物活性

Gp100 (619-627) acetate is amino acids 619 to 627 fragment of human melanoma antigen glycoprotein 100 (gp100). Gp100 has been a widely studied target for melanoma immunotherapy[1].

体外研究
(In Vitro)

The melanocyte differentiation protein, glycoprotein 100 (gp100), encodes a naturally processed epitope that is an attractive target for melanoma immunotherapies, in particular peptide-based vaccines[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1183.38

Formula

C51H86N14O16S

Sequence

Arg-Leu-Met-Lys-Gln-Asp-Phe-Ser-Val

Sequence Shortening

RLMKQDFSV

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献
  • [1]. Bianchi V, et al. A Molecular Switch Abrogates Glycoprotein 100 (gp100) T-cell Receptor (TCR) Targeting of a Human Melanoma Antigen. J Biol Chem. 2016 Apr 22;291(17):8951-9.

Gp100 (619-627)

Gp100 (619-627);

Gp100 (619-627) 是人黑素瘤抗原糖蛋白 100 (gp100) 的氨基酸 619 至 627 片段。Gp100 已被广泛用于黑素瘤免疫疗法的靶标研究。

Gp100 (619-627)amp;;

Gp100 (619-627) Chemical Structure

CAS No. : 187987-68-4

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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生物活性

Gp100 (619-627) is amino acids 619 to 627 fragment of human melanoma antigen glycoprotein 100 (gp100). Gp100 has been a widely studied target for melanoma immunotherapy[1].

体外研究
(In Vitro)

The melanocyte differentiation protein, glycoprotein 100 (gp100), encodes a naturally processed epitope that is an attractive target for melanoma immunotherapies, in particular peptide-based vaccines[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1123.33

Formula

C49H82N14O14S

CAS 号

187987-68-4

Sequence Shortening

RLMKQDFSV

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献
  • [1]. Bianchi V, et al. A Molecular Switch Abrogates Glycoprotein 100 (gp100) T-cell Receptor (TCR) Targeting of a Human Melanoma Antigen. J Biol Chem. 2016 Apr 22;291(17):8951-9.

LCMV GP (61-80)

LCMV GP (61-80);

LCMV GP (61-80) 是衍生自淋巴细胞性脉络丛脑膜炎病毒 (LCMV) 糖蛋白 (GP) 的多肽片段,对应于 61-80 氨基酸序列。LCMV GP (61-80) 是一种特异性抗原决定簇,可以诱导 CD4+ T 细胞反应。

LCMV GP (61-80)amp;;

LCMV GP (61-80) Chemical Structure

CAS No. : 232598-19-5

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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生物活性

LCMV GP (61-80) is a peptide fragment derived from lymphocytic choriomeningitis virus (LCMV) glycoprotein (GP), and corresponds to amino acids 61-80. LCMV GP (61-80) is a specific epitope which can induce CD4+ T-cell response[1][2][3].

分子量

2290.60

Formula

C108H160N24O31

CAS 号

232598-19-5

Sequence

Gly-Leu-Lys-Gly-Pro-Asp-Ile-Tyr-Lys-Gly-Val-Tyr-Gln-Phe-Lys-Ser-Val-Glu-Phe-Asp

Sequence Shortening

GLKGPDIYKGVYQFKSVEFD

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献
  • [1]. Rodriguez F, et, al. CD4(+) T cells induced by a DNA vaccine: immunological consequences of epitope-specific lysosomal targeting. J Virol. 2001 Nov; 75(21): 10421-30.

    [2]. Andargachew R, et, al. CD4 T Cell Affinity Diversity Is Equally Maintained during Acute and Chronic Infection. J Immunol. 2018 Jul 1; 201(1):19-30.

    [3]. Homann D, et, al. Mapping and restriction of a dominant viral CD4+ T cell core epitope by both MHC class I and MHC class II. Virology. 2007 Jun 20; 363(1): 113-23.

Gp100 (25-33), mouse(Synonyms: Mgp100 (25-33))

Gp100 (25-33), mouse;(Synonyms: Mgp100 (25-33))

Gp100 (25-33), mouse 序列位于小鼠自身/肿瘤抗原糖蛋白 (Mgp100) 的第25-33个残基。Mgp100 (25-33), mouse是一种参与色素合成的酶,其表位片段在正常黑色素细胞和黑色素瘤细胞中均有表达。

Gp100 (25-33), mouseamp;;(Synonyms: Mgp100 (25-33))

Gp100 (25-33), mouse Chemical Structure

CAS No. : 212370-36-0

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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Gp100 (25-33), mouse 的其他形式现货产品:

Gp100 (25-33), mouse TFA

生物活性

Gp100 (25-33), mouse sequence is found in residues 25 to 33 of the mouse self/tumor antigen glycoprotein (mgp100). Mgp100 is an enzyme involved in pigment synthesis, and the epitope fragment is expressed in both normal melanocytes and melanoma cells[1].

分子量

1104.13

Formula

C46H69N15O17

CAS 号

212370-36-0

Sequence

Glu-Gly-Ser-Arg-Asn-Gln-Asp-Trp-Leu

Sequence Shortening

EGSRNQDWL

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献
  • [1]. Gal Cafri, et al. Production of LacZ inducible T cell hybridoma specific for human and mouse gp100₂₅₋₃₃ peptides. PLoS One. 2013;8(2):e55583.

GP(33-41)

GP(33-41);

GP(33-41) 是一种由 9 个氨基酸残基组成的多肽,它是淋巴细胞性脉络丛脑膜炎病毒 GP1 抗原决定基中的最佳序列,能够上调 RMA-S (Db Kb) 细胞表面 H-2Db 分子,SC50 值为 344 nM。

GP(33-41)amp;;

GP(33-41) Chemical Structure

CAS No. : 161928-86-5

规格 价格 是否有货
1 mg ¥500 询问价格 货期
5 mg ¥1500 询问价格 货期
10 mg ¥2400 询问价格 货期

* Please select Quantity before adding items.

生物活性

GP(33-41), a 9-aa-long peptide, is the optimal sequence of the GP1 epitope of lymphocytic choriomeningitis virus, and can upregulate H-2Db molecules at the RMA-S (Db Kb) cell surface with a SC50 of 344 nM[1].

体外研究
(In Vitro)

GP(33-41) sensitizes MC57 and T2-Db cells to lysis with ED50s of 0.9±0.6 and 2.5±0.7 nM[1].
The interaction between T cell receptors (TCR) and peptide-major histocompatibility complex (pMHC) antigens can lead to varying degrees of agonism (T cell activation), or antagonism. The P14 TCR recognises the lymphocytic choriomeningitis virus (LCMV)-derived peptide, GP(33-41) (KAVYNFATC), presents in the context of H-2D[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

226.98

Formula

CH3TFKNVY

CAS 号

161928-86-5

Sequence

Lys-Ala-Val-Tyr-Asn-Phe-Ala-Thr-Cys

Sequence Shortening

KAVYNFATC

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Gairin JE, et al. Optimal lymphocytic choriomeningitis virus sequences restricted by H-2Db major histocompatibility complex class I molecules and presented to cytotoxic T lymphocytes. J Virol. 1995 Apr;69(4):2297-305.

    [2]. Boulter JM, et al. Potent T cell agonism mediated by a very rapid TCR/pMHC interaction. Eur J Immunol. 2007 Mar;37(3):798-806.

Kinase Assay
[1]

Binding experiments are performed at 37°C with T2-Db cells, with a Millipore MultiScreen assay system. The H-2Db LCMV antigen gp276-286 (SGVENPGGYCL) is radioiodinated, and the radiolabeled peptide is purified. Cells (2×105 per well) are incubated in MultiScreen-HV 96-well filtration plates (pore size, 0.45 mm) with 125I-gp276-286 (10 nM [final concentration]) for 90 min at 37°C. Cells are washed three times with ice-cold 1% BSA-PBS and by filtration under vacuum. The radioactivity bound to the cells retained on the filter is counted with a gamma counter. Direct binding is measured in the absence (total binding) or the presence (nonspecific binding) of a 1,000-fold excess (10 mM) of unlabeled gp276-286. Specific binding to H-2Db is defined as the difference between total binding and nonspecific binding. Nontransfected T2 cells are used as a negative control under the same experimental conditions. Competition assays are performed with increasing concentrations (10-10 to 10-5 M) of unlabeled peptides competing against a fixed concentration (10-8 M) of 125Igp276-286. The percent inhibition of binding is calculated as 100 × [1-(counts per minute in the presence of competitor – counts per minute of nonspecific binding)/counts per minute of specific binding].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Gairin JE, et al. Optimal lymphocytic choriomeningitis virus sequences restricted by H-2Db major histocompatibility complex class I molecules and presented to cytotoxic T lymphocytes. J Virol. 1995 Apr;69(4):2297-305.

    [2]. Boulter JM, et al. Potent T cell agonism mediated by a very rapid TCR/pMHC interaction. Eur J Immunol. 2007 Mar;37(3):798-806.

SCGP00525-Millipore Steriflip-GP聚醚砜无菌过滤器

  • 型号 SCGP00525
  • 品牌 Merck Millipore密理博
  • 【简单介绍】

    Millipore Steriflip-GP聚醚砜无菌过滤器
    滤膜孔径: 0.22µm

    可润湿性: 亲水

    残留体积: 600µL

    灭菌方式: γ 射线照射

    产品直径: 64mm

    滤膜直径: 40mm

    产品高度: 138mm

    是否无菌: 无菌

    过滤面积: 7cm2

    Steriflip-GP详细资料:

    产品说明: Steriflip-GP,0.22µm,改良聚醚砜,已经 γ 射线照射

    产品应用: 对组织培养基和添加剂、蛋白质溶液、病毒悬浮液、DNA 及其它水溶液进行除菌过滤

    进口接头: 带真空口的双螺纹

    滤膜材质: 改良聚醚砜

    产品颜色: 无色

    接收装置: 50 mL 离心管,带盖

    出口接头: 50 mL 双螺纹离心管

    zui高操作温度: 45°C

    处理体积: 50mL

    滤膜孔径: 0.22µm

    可润湿性: 亲水

    残留体积: 600µL

    灭菌方式: γ 射线照射

    产品直径: 64mm

    滤膜直径: 40mm

    产品高度: 138mm

    是否无菌: 无菌

    过滤面积: 7cm2

    装置材质: MBS/聚丙烯

    产品参数:Steriflip 可与标准的50ml离心管(如Greiner227270)一起使用,这样可避免样品的转移。过滤时只要将该装置与盛有样品的50ml离心管连接起来,倒置真空过滤便可。过滤结束时,过滤液收集在相连的50ml离心管中。

    Millipore Steriflip-GP聚醚砜无菌过滤器 SCGP00525订货信息:

    产品编号      产品描述                  产品包装 
    SE1M005M00 滤膜类型 PES;孔径:0.1μm    25个/盒     
    SCGP00525  滤膜类型 PES;孔径:0.22μm   25个/盒        
    SE1M179M6  滤膜类型 PVDF;孔径:0.22μm  25个/盒        
    SE1M003M00 滤膜类型 PVDF;孔径:0.45μm  25个/盒    
    SE1M002M8  滤膜类型 NYLON;孔径:100μm  25个/盒     
    SC50FL025  滤膜类型 50ml漏斗;          25个/盒 

MilliporeMillex-GP针头滤器

MilliporeMillex-GP针头滤器

Millipore Millex-GP针头滤器

产品编号:
市场价:¥0.00
会员价:¥0.00
品牌:Millipore 密理博
生产厂家:Millipore
需求数量:

  • 产品概览
  • 技术参数
  • 订购信息
  • 相关资料
  • 相关产品

Millipore Millex-GP针头滤器

说明:Millex-GP0.22um,改良聚醚砜,33mm,射线灭菌

商标名:Millex

数量/包装:50

高度:26

应用:对组织培养基盒添加剂、蛋白溶液、病毒悬混液、DNA及其他水溶液进行除菌过滤。

过滤商标名:Millipore Express

装置颜色:绿色

进口接头:阴Luer-Lok接头

出口接头:阳luer滑动接头

过滤面积,㎝24.5

滤膜直径,mm33

直径,mm33

灭菌:V射线照射

装置材料:改良聚醚

滤膜孔径,um0.22

可湿润性:亲水

最大进口压力,bar(psi):≤10145

处理体积,ml200

millipore代理millipore

LCMV gp33-41

LCMV gp33-41; 纯度: 98.09%

LCMV gp33-41,羧基延伸的11-aa-长肽,受 MHC I 类 H-2Db 分子限制的淋巴细胞脉络丛脑膜炎病毒序列,可呈递给细胞毒性 T 淋巴细胞。

LCMV gp33-41 

LCMV gp33-41 Chemical Structure

CAS No. : 151705-84-9

规格 价格 是否有货 数量
1 mg ¥900 In-stock
5 mg ¥1900 In-stock
10 mg ¥2900 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

LCMV gp33-41 相关产品

相关化合物库:

  • Bioactive Compound Library Plus
  • Immunology/Inflammation Compound Library
  • Peptide Library

生物活性

LCMV gp33-41, the carboxyl-extended 11-aa-long peptide, is an lymphocytic choriomeningitis virus sequence restricted by MHC class I H-2Db molecules and presented to cytotoxic T lymphocytes[1].

分子量

1044.22

Formula

FTKNVY

CAS 号

151705-84-9

Sequence

Lys-Ala-Val-Tyr-Asn-Phe-Ala-Thr-Met

Sequence Shortening

KAVYNFATM

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -80°C 2 years
-20°C 1 year
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro:;

H2O : 12.5 mg/mL (11.97 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.9577 mL 4.7883 mL 9.5765 mL
5 mM 0.1915 mL 0.9577 mL 1.9153 mL
10 mM 0.0958 mL 0.4788 mL 0.9577 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Gairin JE, et al. Optimal lymphocytic choriomeningitis virus sequences restricted by H-2Db major histocompatibility complex class I molecules and presented to cytotoxic T lymphocytes. J Virol. 1995 Apr;69(4):2297-305.

LCMV gp33-41 TFA

LCMV gp33-41 TFA; 纯度: 99.0%

LCMV gp33-41 (TFA),羧基延伸的 11-aa-长肽,受 MHC I 类 H-2Db 分子限制的淋巴细胞脉络丛脑膜炎病毒序列,可呈递给细胞毒性 T 淋巴细胞。

LCMV gp33-41 TFAamp;;

LCMV gp33-41 TFA Chemical Structure

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ¥3690 In-stock
1 mg ¥900 In-stock
5 mg ¥1900 In-stock
10 mg ¥2900 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

LCMV gp33-41 TFA 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Immunology/Inflammation Compound Library
  • Peptide Library

生物活性

LCMV gp33-41 (TFA), the carboxyl-extended 11-aa-long peptide, is an lymphocytic choriomeningitis virus sequence restricted by MHC class I H-2Db molecules and presented to cytotoxic T lymphocytes[1].

分子量

1158.24

Formula

C50H74N11F3015S

Sequence

Lys-Ala-Val-Tyr-Asn-Phe-Ala-Thr-Met

Sequence Shortening

KAVYNFATM

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro:;

DMSO : 100 mg/mL (86.34 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.8634 mL 4.3169 mL 8.6338 mL
5 mM 0.1727 mL 0.8634 mL 1.7268 mL
10 mM 0.0863 mL 0.4317 mL 0.8634 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (2.16 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (2.16 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (2.16 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (2.16 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂:;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.5 mg/mL (2.16 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (2.16 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Gairin JE, et al. Optimal lymphocytic choriomeningitis virus sequences restricted by H-2Db major histocompatibility complex class I molecules and presented to cytotoxic T lymphocytes. J Virol. 1995 Apr;69(4):2297-305.

SLGPB5010-默克 密理博Millex-GP无菌过滤器0.22um孔径

  • 型号 SLGPB5010
  • 品牌 Merck Millipore密理博
  • 【简单介绍】

    默克 密理博Millex-GP无菌过滤器0.22um孔径 SLGPB5010
    商标名称: Millex
    滤膜材料: 改良聚醚砜
    产品说明: Millex-GP,0.22μm,改良聚醚砜,50mm,HB/HB 带钟罩,射线灭菌
    产品应用: 对组织培养基和添加剂、蛋白质溶液、病毒悬浮液、DNA 及其它水溶液进行除菌过滤
    进口接头: 阶形倒刺软管接口,带阴 Luer 内滑动接头

    默克 密理博Millex-GP无菌过滤器0.22um孔径 SLGPB5010

     

    产品说明: Millex-GP,0.22μm,改良聚醚砜,50mm,HB/HB 带钟罩,射线灭菌

    商标名称: Millex

    产品应用: 对组织培养基和添加剂、蛋白质溶液、病毒悬浮液、DNA 及其它水溶液进行除菌过滤

    进口接头: 阶形倒刺软管接口,带阴 Luer 内滑动接头

    滤膜材料: 改良聚醚砜

    滤器色码: 进/出口均为自然色

    滤膜商标: Millipore Express

    进口压力: 3.5bar

    出口接头: 钟罩

    zui高操作温度: 45°C

    处理体积: 4000mL

    滤膜孔径: 0.22μm

    可润湿性: 亲水

    残留体积: <1000ul

    灭菌方式: γ 射线照射

    外壳直径: 62mm

    滤膜直径: 50mm

    整体高度: 96mm

    是否无菌: 无菌

    过滤面积: 19.6cm2

    产品材质: MBS

    产品名称: 无菌过滤器

    产品参数:Steriflip 可与标准的50ml离心管(如Greiner227270)一起使用,这样可避免样品的转移。过滤时只要将该装置与盛
    有样品的50ml离心管连接起来,倒置真空过滤便可。过滤结束时,过滤液收集在相连的50ml离心管中。

    默克 密理博Millex-GP无菌过滤器0.22um孔径 SLGPB5010
    产品编号      产品描述                  产品包装 
    SE1M005M00 滤膜类型 PES;孔径:0.1μm    25个/盒     
    SCGP00525  滤膜类型 PES;孔径:0.22μm   25个/盒        
    SE1M179M6  滤膜类型 PVDF;孔径:0.22μm  25个/盒        
    SE1M003M00 滤膜类型 PVDF;孔径:0.45μm  25个/盒    
    SE1M002M8  滤膜类型 NYLON;孔径:100μm  25个/盒     
    SC50FL025  滤膜类型 50ml漏斗;          25个/盒