HA Peptide

HA Peptide; 纯度: 99.42%

HA Peptide (HA tag) 是来自人类流感血凝素 (HA) 的由九个氨基酸组成的多肽。HA Peptide 广泛用于细胞生物学和生物化学中分离,纯化,检测和追踪目标蛋白。

HA Peptideamp;;

HA Peptide Chemical Structure

CAS No. : 92000-76-5

规格 价格 是否有货 数量
1 mg ¥500 In-stock
5 mg ¥990 In-stock
10 mg ¥1650 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

HA Peptide 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

HA Peptide (HA tag) is a nine amino acids peptide derived from the human influenza hemagglutinin (HA). HA Peptide is extensively used to isolate, purify, detect, and track the protein of interest in cell biology and biochemistry.

体外研究
(In Vitro)

HA Peptide is a highly immunoreactive tag generally used for the separation of tagged proteins from cell culture supernatants and cell lysate under neutral pH conditions and thus are handy tools for coimmunoprecipitation but are also easily detected via western blot. HA Peptide is small and thus unlikely to interfere with the bioactivity and function of the fusion partner proteins. HA Peptide comes from human influenza hemagglutinin (HA) corresponding to amino acids 98–106 and is a strong immunoreactive epitope making it popular to isolate, purify, detect, and track the protein of interest. The recombinant HA-tagged proteins can be separated by highly specific anti-HA monoclonal antibody that is covalently immobilized on resin. The HA-tagged proteins can be eluted by mild elution approach with HA epitope at 1 mg/mL in TBS. On the other hand, three chemical elution options are available: 0.1 M glycine (pH 2-2.8), 3 M NaSCN, or 50 mM NaOH[1]. The nucleotide sequences encoding an N-terminal HA Peptide in the mammalian expression vectors is an essential element for the T7 promoter-driven expression in E. coli even without trans-acting T7 RNAP[2]. Research results suggest that HA Peptide is cleaved by caspase 3/7, and HA Peptide cleavage results in a total loss of immunoreactivity. Observations indicate that the use of HA to tag proteins and constructs to study cell death–related and apoptotic mechanisms can result in serious artifacts[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1102.15

Formula

D2P2VY3

CAS 号

92000-76-5

Sequence Shortening

YPYDVPDYA

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

H2O : 50 mg/mL (45.37 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.9073 mL 4.5366 mL 9.0732 mL
5 mM 0.1815 mL 0.9073 mL 1.8146 mL
10 mM 0.0907 mL 0.4537 mL 0.9073 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Zhao X, et al. Several affinity tags commonly used in chromatographic purification. J Anal Methods Chem. 2013;2013:581093.

    [2]. Moon JM, et al. A new idea for simple and rapid monitoring of gene expression: requirement of nucleotide sequences encoding an N-terminal HA tag in the T7 promoter-driven expression in E. coli. Biotechnol Lett. 2012 Oct;34(10):1841-6.

    [3]. Schembri L, et al. The HA tag is cleaved and loses immunoreactivity during apoptosis. Nat Methods. 2007 Feb;4(2):107-8.

密理博Millex-HA针头过滤器SLHA033SB

密理博Millex-HA针头过滤器

简要描述:

 

33mm Millex 过滤器概述 
无菌 Millex 过滤器现在采用一种更大的新外壳,达 33mm,装有 MCE、PVDF 或 PES 膜。 
流速更快 
更大的滤膜表面积提高了流速和处理量。 它还降低了排空注射器所需要的压力,从而使过滤溶液更容易。 
操作压力更高 
新型Millex 过滤器的大外壳压力 10.5 bar (150 psig),这意味着您可以比以前更快地过滤溶液。 
颜色编码 
新型 Millex 外壳上的颜色外圈条清楚地标明了里面滤膜的类型。 
性能可靠 
新型 Millex 过滤器的制造是在一个可控的环境和自动过程中进行的。 在装配过程中,操作人员的手决不会接触该过滤器。 每个盒中附带的“质量证书”详细地描述了 Millipore 的质量标准。 
滤膜的选择 
产品详细信息
33mm无菌Millex过滤器
Millex-HP,0.45 µm,改良聚醚砜,33 mm,射线灭菌
商标名: Millex
进口接头: 阴 Luer-Lok 接头
色码: 绿色
滤膜商标名: Millipore Express PLUS
*大进口压力,bar (psi): ≤10 (145)
直接病人护理,是/否: Y
出口接头: 阳 luer 滑动接头
处理体积,mL: 200
滤膜孔径,µm: 0.45
可润湿性: 亲水
灭菌: γ 射线照射
套筒: Modified Acrylic
直径,mm: 33
滤膜直径,mm: 33
高度,mm: 26
无菌: 无菌
过滤面积,cm2: 4.5
装置材质: 改良丙烯酸

SLHP033RS  50个/盒
SLHP033RB  250个/盒
    ◆新型 Millex 过滤器装有三种膜:Millipore Express® (PES) 膜、混合纤维素酯 (MCE) 及 Durapore (PVDF)。 
    ◆Millex-GP 过滤器采用 Millipore Express (PES) 膜,提供快速和低蛋白质吸附(为竞争 PES 膜的六分之一)的*组合。 建议在除菌过滤蛋白质溶液、组织培养基和添加剂中使用 Millex-GP 过滤器。 
    ◆装有 Durapore® (PVDF) 膜的 Millex 过滤器是针头式过滤器中蛋白质吸附低的。 请使用该过滤器来过滤蛋白质溶液。 0.22µm 孔径为除菌级过滤器。 孔径更大的滤膜用于澄清和预过滤。 
    ◆Millex-MF 过滤器采用混合纤维素酯 (MCE) 膜,它可以对水、缓冲液或其它水溶液进行可靠的常规过滤。 0.2µm 孔径为除菌级过滤器。 孔径更大的滤膜用于澄清和预过滤。
详细说明:

应用范围:
HPLC,MS,LC-MS,GC-MS等分析仪器的样品制备,纯化,前处理。
生物制品制备。
组织培养基、微生物介质、缓冲溶液等液体样品的过滤。
腐蚀性强的溶液的净化、澄清。
少量样品的过滤,除去样品中杂质,沉淀。

技术特点:
一次性针头式过滤器均采用进口微孔滤膜,对样品的吸附性小,保证少量或高价值样品的大回收。针头式过滤器外壳采用溶出低、化学相容性好的高密聚乙烯(HDPE)材料,采用用超声波焊接,不用任何胶质物或密封剂,不会污染样品。
提供混合纤维素膜、尼龙膜、聚偏氟乙烯PVDF膜的一次性针头式样品过滤器,满足用户对于过滤样品量、化学相容性的不同要求。
有机相/有机系针头式过滤器采用NYLON(尼龙)微孔滤膜,化学兼容性好,对一般HPLC有机溶液甲醇,乙腈,正己烷,异丙醇等都具有的化学抗溶性。可用于过滤有机溶剂样品,也可过滤水相溶剂样品。
水相/水系针头式过滤器采用混合纤维素酯Mix cellulose (MCE)微孔滤膜。用于过滤水系溶液样品,不适用于过滤有机溶剂样品。
一次性针头式过滤器,使得水溶液和有机溶液都能快速而有效地过滤。
技术指标:
外壳:聚丙烯
膜材质:混合纤维素膜、尼龙膜、聚偏氟乙烯PVDF膜

密理博Millex-HA针头过滤器

 

密理博Millex-HA针头过滤器SLHA033SB密理博Millex-HA针头过滤器SLHA033SB

HA Peptide TFA

HA Peptide TFA  纯度: 99.21%

HA Peptide (TFA) 是来自人类流感血凝素 (HA) 的由九个氨基酸组成的多肽。HA Peptide (TFA) 广泛用于细胞生物学和生物化学中分离,纯化,检测和追踪目标蛋白。

HA Peptide TFA 

HA Peptide TFA Chemical Structure

规格 价格 是否有货 数量
5 mg ¥900 In-stock
10 mg ¥1500 In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

HA Peptide TFA 相关产品

相关化合物库:

  • Bioactive Compound Library Plus
  • Immunology/Inflammation Compound Library
  • Peptide Library

生物活性

HA Peptide (TFA) is a nine amino acids peptide derived from the human influenza hemagglutinin (HA). HA Peptide (TFA) is extensively used to isolate, purify, detect, and track the protein of interest in cell biology and biochemistry[1][2][3].

体外研究
(In Vitro)

HA Peptide is a highly immunoreactive tag generally used for the separation of tagged proteins from cell culture supernatants and cell lysate under neutral pH conditions and thus are handy tools for coimmunoprecipitation but are also easily detected via western blot. HA Peptide is small and thus unlikely to interfere with the bioactivity and function of the fusion partner proteins. HA Peptide comes from human influenza hemagglutinin (HA) corresponding to amino acids 98-106 and is a strong immunoreactive epitope making it popular to isolate, purify, detect, and track the protein of interest. The recombinant HA-tagged proteins can be separated by highly specific anti-HA monoclonal antibody that is covalently immobilized on resin. The HA-tagged proteins can be eluted by mild elution approach with HA epitope at 1 mg/mL in TBS. On the other hand, three chemical elution options are available: 0.1 M glycine (pH 2-2.8), 3 M NaSCN, or 50 mM NaOH[1]. The nucleotide sequences encoding an N-terminal HA Peptide in the mammalian expression vectors is an essential element for the T7 promoter-driven expression in E. coli even without trans-acting T7 RNAP[2]. Research results suggest that HA Peptide is cleaved by caspase 3/7, and HA Peptide cleavage results in a total loss of immunoreactivity. Observations indicate that the use of HA to tag proteins and constructs to study cell death–related and apoptotic mechanisms can result in serious artifacts[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1216.19

Formula

C55H68F3N9O19

Sequence Shortening

YPYDVPDYA

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献
  • [1]. Zhao X, et, al. Several affinity tags commonly used in chromatographic purification. J Anal Methods Chem. 2013;2013:581093.

    [2]. Moon JM, et, al. A new idea for simple and rapid monitoring of gene expression: requirement of nucleotide sequences encoding an N-terminal HA tag in the T7 promoter-driven expression in E. coli. Biotechnol Lett. 2012 Oct;34(10):1841-6.

    [3]. Schembri L, et, al. The HA tag is cleaved and loses immunoreactivity during apoptosis. Nat Methods. 2007 Feb;4(2):107-8.

  • [1]. Zhao X, et, al. Several affinity tags commonly used in chromatographic purification. J Anal Methods Chem. 2013;2013:581093.

    [2]. Moon JM, et, al. A new idea for simple and rapid monitoring of gene expression: requirement of nucleotide sequences encoding an N-terminal HA tag in the T7 promoter-driven expression in E. coli. Biotechnol Lett. 2012 Oct;34(10):1841-6.

    [3]. Schembri L, et, al. The HA tag is cleaved and loses immunoreactivity during apoptosis. Nat Methods. 2007 Feb;4(2):107-8.

Anti HA Antibody Beads 抗HA抗体珠 品牌:FUJIFILM Wako


Anti HA Antibody Beads

抗HA抗体珠

品牌:FUJIFILM Wako
CAS No.:
储存条件:-20℃
纯度:
产品编号

(生产商编号)

等级 规格 运输包装 零售价(RMB) 库存情况 参考值

010-23083

for Immunochemistry 10mL(Net 5mL) 20,360.00


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动,恕不另行通知,确切详情请联系上海金畔生物科技有限公司。

MiTeGen B-M8-HA1-B5 MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B5 bases.

上海金畔生物科技有限公司提供MiTeGen B-M8-HA1-B5 MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B5 bases.,欢迎访问官网了解更多产品信息和订购

品牌 货号 产品名称 规格 价格

MiTeGen B-M8-HA1-B5 MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B5 bases. 0.00

MiTeGen B-M8-HA1-B3S-R MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B3S-R reusable bases. 0.00

MiTeGen B-M8-HA1-B3S MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B3S bases. 0.00

MiTeGen B-M8-HA1-B3-R MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B3-R reusable bases. 0.00

MiTeGen B-M8-HA1-B3 MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B3 bases. 0.00

MiTeGen B-M8-HA1-B1-R MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B1-R reusable bases. 0.00

MiTeGen B-M8-HA1-B1A-R MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B1A-R reusable bases. 0.00

MiTeGen B-M8-HA1-B1A MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B1A bases. 0.00

MiTeGen B-M8-HA1-B1 MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B1 bases. 0.00

MiTeGen B-M8-70V-B5-R MicroLoop E Assembly; box of 20 assemblies, vertical MicroLoop E, 20 of 70 x 700 micron Mounts on 18mm/SPINE length rods adhered into B5-R reusable bases. 0.00

MiTeGen B-M8-70V-B5 MicroLoop E Assembly; box of 20 assemblies, vertical MicroLoop E, 20 of 70 x 700 micron Mounts on 18mm/SPINE length rods adhered into B5 bases. 0.00

MiTeGen B-M8-70V-B3S-R MicroLoop E Assembly; box of 20 assemblies, vertical MicroLoop E, 20 of 70 x 700 micron Mounts on 18mm/SPINE length rods adhered into B3S-R reusable bases. 0.00

MiTeGen B-M8-70V-B3S MicroLoop E Assembly; box of 20 assemblies, vertical MicroLoop E, 20 of 70 x 700 micron Mounts on 18mm/SPINE length rods adhered into B3S bases. 0.00

MiTeGen B-M8-70V-B3-R MicroLoop E Assembly; box of 20 assemblies, vertical MicroLoop E, 20 of 70 x 700 micron Mounts on 18mm/SPINE length rods adhered into B3-R reusable bases. 0.00

MiTeGen B-M8-70V-B3 MicroLoop E Assembly; box of 20 assemblies, vertical MicroLoop E, 20 of 70 x 700 micron Mounts on 18mm/SPINE length rods adhered into B3 bases. 0.00

MiTeGen B-M8-70V-B1-R MicroLoop E Assembly; box of 20 assemblies, vertical MicroLoop E, 20 of 70 x 700 micron Mounts on 18mm/SPINE length rods adhered into B1-R reusable bases. 0.00

MiTeGen B-M8-70V-B1A-R MicroLoop E Assembly; box of 20 assemblies, vertical MicroLoop E, 20 of 70 x 700 micron Mounts on 18mm/SPINE length rods adhered into B1A-R reusable bases. 0.00

MiTeGen B-M8-70V-B1A MicroLoop E Assembly; box of 20 assemblies, vertical MicroLoop E, 20 of 70 x 700 micron Mounts on 18mm/SPINE length rods adhered into B1A bases. 0.00

MiTeGen B-M8-70V-B1 MicroLoop E Assembly; box of 20 assemblies, vertical MicroLoop E, 20 of 70 x 700 micron Mounts on 18mm/SPINE length rods adhered into B1 bases. 0.00

MiTeGen B-M8-50V-B5-R MicroLoop E Assembly; box of 20 assemblies, vertical MicroLoop E, 20 of 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B5-R reusable bases. 0.00

anti-Phospho-Histone H2A/H4 (Ser1), Rabbit Monoclonal (RM216) 抗磷酸化组蛋白 H2A/H4 (Ser1), 兔单克隆抗体 (RM216) 品牌:RevMab


anti-Phospho-Histone H2A/H4 (Ser1), Rabbit Monoclonal (RM216)

抗磷酸化组蛋白 H2A/H4 (Ser1), 兔单克隆抗体 (RM216)

品牌:RevMab
CAS No.:
储存条件:-20°C
纯度:Protein A purified.
产品编号

(生产商编号)

等级 规格 运输包装 零售价(RMB) 库存情况 参考值

REV-31-1092-00-C100

100 µg 6,570.00


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动,恕不另行通知,确切详情请联系上海金畔生物科技有限公司。

anti-Acetyl-Histone H2A.Z (Lys4), Rabbit Monoclonal (RM221) 抗乙酰化组蛋白 H2A.Z (Lys4), 兔单克隆抗体 (RM221) 品牌:RevMab


anti-Acetyl-Histone H2A.Z (Lys4), Rabbit Monoclonal (RM221)

抗乙酰化组蛋白 H2A.Z (Lys4), 兔单克隆抗体 (RM221)

品牌:RevMab
CAS No.:
储存条件:-20°C
纯度:Protein A purified.
产品编号

(生产商编号)

等级 规格 运输包装 零售价(RMB) 库存情况 参考值

REV-31-1101-00-C100

100 µg 6,570.00


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动,恕不另行通知,确切详情请联系上海金畔生物科技有限公司。

anti-Acetyl-Histone H2A.Z (Lys7), Rabbit Monoclonal (RM222) 抗乙酰化组蛋白 H2A.Z (Lys7), 兔单克隆抗体 (RM222) 品牌:RevMab


anti-Acetyl-Histone H2A.Z (Lys7), Rabbit Monoclonal (RM222)

抗乙酰化组蛋白 H2A.Z (Lys7), 兔单克隆抗体 (RM222)

品牌:RevMab
CAS No.:
储存条件:-20°C
纯度:Protein A purified.
产品编号

(生产商编号)

等级 规格 运输包装 零售价(RMB) 库存情况 参考值

REV-31-1102-00-C100

100 µg 6,570.00


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动,恕不另行通知,确切详情请联系上海金畔生物科技有限公司。

anti-Core Histone Macro-H2A.1 (H2AFY), Rabbit Monoclonal (RM248) 抗核心组蛋白 Macro-H2A.1 (H2AFY), 兔单克隆抗体 (RM248) 品牌:RevMab


anti-Core Histone Macro-H2A.1 (H2AFY), Rabbit Monoclonal (RM248)

抗核心组蛋白 Macro-H2A.1 (H2AFY), 兔单克隆抗体 (RM248)

品牌:RevMab
CAS No.:
储存条件:-20°C
纯度:Protein A purified.
产品编号

(生产商编号)

等级 规格 运输包装 零售价(RMB) 库存情况 参考值

REV-31-1118-00-C100

100 µg 6,570.00


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动,恕不另行通知,确切详情请联系上海金畔生物科技有限公司。

anti-Acetyl-Histone H2A.X (Lys5), Rabbit Monoclonal (RM445) 抗乙酰化组蛋白 H2A.X (Lys5), 兔单抗 (RM445) 品牌:RevMab


anti-Acetyl-Histone H2A.X (Lys5), Rabbit Monoclonal (RM445)

抗乙酰化组蛋白 H2A.X (Lys5), 兔单抗 (RM445)

品牌:RevMab
CAS No.:
储存条件:-20°C
纯度:Protein A purified.
产品编号

(生产商编号)

等级 规格 运输包装 零售价(RMB) 库存情况 参考值

REV-31-1336-00-C025

25 µg 2,950.00


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动,恕不另行通知,确切详情请联系上海金畔生物科技有限公司。