荧光染料Dye Reagents Hydroxyphenyl Fluorescein;(Synonyms: HPF)
Hydroxyphenyl fluorescein (HPF) 是一种可直接检测高活性氧 (hROS) 的试剂。Hydroxyphenyl fluorescein 选择性和剂量依赖性地与 hROS 反应,例如显示强荧光的羟基自由基和过氧亚硝酸盐。
Hydroxyphenyl Fluorescein Chemical Structure
CAS No. : 359010-69-8
规格 | 价格 | 是否有货 | |
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1 mg | ¥10800 | 询问价格 货期 |
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生物活性 |
Hydroxyphenyl fluorescein (HPF) is the reagent that can directly detect highly reactive oxygen species (hROS). Hydroxyphenyl fluorescein selectively and dose-dependently reacts with hROS, such as the hydroxyl radical and peroxynitrite, which exhibit strong fluorescence[1][2]. |
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体外研究 (In Vitro) |
The fluorescence of Hydroxyphenyl Fluorescein (HPF) is greatly enhanced following incubation with blue light illuminated blebbistatin (30 min). Fluorescence enhancement of Hydroxyphenyl Fluorescein occurred equally efficiently under Ar purged and non-Ar purged conditions, suggesting that singlet oxygen as an intermediate involved is improbable. Fluorescence measurement with Hydroxyphenyl Fluorescein suggests a negative linear relationship between fluorescence intensity of Hydroxyphenyl Fluorescein (10 μM) and thiourea concentration (0-1 mM)[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
体内研究 (In Vivo) |
The Hydroxyphenyl Fluorescein (HPF) probe (15 μl, 25 μM) is given intratesticularly in anaesthetized mice 20 min before IR (ionizing radiation). The accumulation of •OH by the fluorescence signal emitted by the oxidized form of HPF is assessed[3]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
分子量 |
424.40 |
Formula |
C26H16O6 |
CAS 号 |
359010-69-8 |
中文名称 |
羟苯基荧光素 |
运输条件 |
Room temperature in continental US; may vary elsewhere. |
储存方式 |
Please store the product under the recommended conditions in the Certificate of Analysis. |
参考文献 |
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