Desaminotyrosine(Synonyms: 对羟基苯丙酸; 3-(4-Hydroxyphenyl)propionic acid)

天然产物 天然产物苯丙素类 Phenylpropanoids

Desaminotyrosine (Synonyms: 对羟基苯丙酸; 3-(4-Hydroxyphenyl)propionic acid) 纯度: 99.81%

Desaminotyrosine 是一种微生物相关的代谢物,通过扩大 I 型干扰素 (type I interferon) 信号来阻止 influenza 的发生。

Desaminotyrosine(Synonyms: 对羟基苯丙酸; 3-(4-Hydroxyphenyl)propionic acid)

Desaminotyrosine Chemical Structure

CAS No. : 501-97-3

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Free Sample (0.1-0.5 mg)   Apply now  
10 mM * 1 mL in DMSO ¥550 In-stock
100 mg ¥500 In-stock
200 mg   询价  
500 mg   询价  

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Desaminotyrosine 相关产品

相关化合物库:

  • Natural Product Library Plus
  • Bioactive Compound Library Plus
  • Anti-Infection Compound Library
  • Metabolism/Protease Compound Library
  • Natural Product Library
  • Antiviral Compound Library
  • Human Endogenous Metabolite Compound Library
  • Fragment Library
  • Phenols Library
  • Gut Microbial Metabolite Library
  • Microbial Metabolite Library
  • Food-Sourced Compound Library

生物活性

Desaminotyrosine is a microbially associated metabolite protecting from influenza through augmentation of type I interferon signaling.

IC50 & Target

Microbial Metabolite

 

Human Endogenous Metabolite

 

体内研究
(In Vivo)

Wild-type mice produce nanomoles of Desaminotyrosine per gram of feces and have picomolar quantities of Desaminotyrosine in the serum. Desaminotyrosine (200 mM) treatment before influenza infection, protects from influenza by type I interferon (IFN) signaling, with no effect on weight loss or survival in mice with or without vancomycin, neomycin, ampicillin, and metronidazole (VNAM). Desaminotyrosine enhances type I IFN in macrophages via type I IFN amplification in mice[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

166.18

Formula

C9H10O3

CAS 号

501-97-3

中文名称

根皮酸;对羟基苯丙酸

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO : 100 mg/mL (601.76 mM; Need ultrasonic)

H2O : 33.33 mg/mL (200.57 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 6.0176 mL 30.0879 mL 60.1757 mL
5 mM 1.2035 mL 6.0176 mL 12.0351 mL
10 mM 0.6018 mL 3.0088 mL 6.0176 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: PBS

    Solubility: 100 mg/mL (601.76 mM); Clear solution; Need ultrasonic

  • 2.

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (15.04 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (15.04 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 3.

    请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (15.04 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (15.04 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 4.

    请依序添加每种溶剂: 10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (15.04 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (15.04 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Steed AL, et al. The microbial metabolite desaminotyrosine protects from influenza through type I interferon. Science. 2017 Aug 4;357(6350):498-502.

Animal Administration
[1]

Mice[1]
Indicated mice receive 200 mM Desaminotyrosine dissolved in Kool-Aid in the drinking water (control animals receive Kool-Aid alone). Indicated mice receive poly(IC) injections of 1 mg/kg for vancomycin, neomycin, ampicillin, and metronidazole (VNAM) treated mice or 5 mg/kg for all other mice intraperitoneally daily for 4 days, and then mice are scarified on day five at which time serum is collected[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Steed AL, et al. The microbial metabolite desaminotyrosine protects from influenza through type I interferon. Science. 2017 Aug 4;357(6350):498-502.

荧光染料Hydroxyphenyl Fluorescein(Synonyms: HPF)

荧光染料Dye Reagents Hydroxyphenyl Fluorescein;(Synonyms: HPF)

Hydroxyphenyl fluorescein (HPF) 是一种可直接检测高活性氧 (hROS) 的试剂。Hydroxyphenyl fluorescein 选择性和剂量依赖性地与 hROS 反应,例如显示强荧光的羟基自由基和过氧亚硝酸盐。

Hydroxyphenyl Fluorescein(Synonyms: HPF)

Hydroxyphenyl Fluorescein Chemical Structure

CAS No. : 359010-69-8

规格 价格 是否有货
1 mg ¥10800 询问价格 货期

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生物活性

Hydroxyphenyl fluorescein (HPF) is the reagent that can directly detect highly reactive oxygen species (hROS). Hydroxyphenyl fluorescein selectively and dose-dependently reacts with hROS, such as the hydroxyl radical and peroxynitrite, which exhibit strong fluorescence[1][2].

体外研究
(In Vitro)

The fluorescence of Hydroxyphenyl Fluorescein (HPF) is greatly enhanced following incubation with blue light illuminated blebbistatin (30 min). Fluorescence enhancement of Hydroxyphenyl Fluorescein occurred equally efficiently under Ar purged and non-Ar purged conditions, suggesting that singlet oxygen as an intermediate involved is improbable. Fluorescence measurement with Hydroxyphenyl Fluorescein suggests a negative linear relationship between fluorescence intensity of Hydroxyphenyl Fluorescein (10 μM) and thiourea concentration (0-1 mM)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

The Hydroxyphenyl Fluorescein (HPF) probe (15 μl, 25 μM) is given intratesticularly in anaesthetized mice 20 min before IR (ionizing radiation). The accumulation of •OH by the fluorescence signal emitted by the oxidized form of HPF is assessed[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

424.40

Formula

C26H16O6

CAS 号

359010-69-8

中文名称

羟苯基荧光素

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Ming-De Li, et al. Dynamics of Oxygen-Independent Photocleavage of Blebbistatin as a One-Photon Blue or Two-Photon Near-Infrared Light-Gated Hydroxyl Radical Photocage. J Am Chem Soc. 2018 Nov 21;140(46):15957-15968.

    [2]. Yoichiro Masuda, et al. Free radical production by femtosecond laser lens irradiation in porcine eyes. J Cataract Refract Surg. 2019 Aug;45(8):1168-1171.

    [3]. Yunhai Chuai, et al. Hydrogen-rich saline attenuates radiation-induced male germ cell loss in mice through reducing hydroxyl radicals. Biochem J. 2012 Feb 15;442(1):49-56.