- 型号 IPVH00010
- 品牌 Merck Millipore密理博
- 【简单介绍】
Millipore密理博PVDF膜 0.45um孔径 转印膜 IPVH00010
Millipore密理博 Immobilon转印膜IPVH00010
规格:孔径0.45μm PVDF膜
尺寸:26.5cm X 3.75mMillipore密理博PVDF膜 0.45um孔径 转印膜 IPVH00010
Millipore密理博 Immobilon转印膜IPVH00010 规格:孔径0.45μm PVDF膜 尺寸:26.5cm X 3.75m Millipore密理博 Immobilon转印膜IPVH00010
产品详细信息
Millipore密理博 mmobilon 转印膜 IPVH00010尺寸
过滤器表面
光面
过滤器尺寸
26.5cm x 3.75m 孔径;0.45μm
材料信息
化学
Hydrophobic PVDF
生物信息
主结合机制
静电,疏水
BSA吸收性(µg/cm²)
215
山羊IgG吸收性(µg/cm²)
294
胰岛素吸收性(µg/cm²)
160
可湿润性
疏水
Millipore 提供三种不同的Immobilon PVDF膜,是针对不同的蛋白质印迹应用的理想选择。现在我们还提供采用按规格裁切的印迹方片膜和两层过滤纸组成的印迹三明治。
与硝酸纤维素膜相比,Immobilon膜具有多种优点,它们不易破裂或卷曲,便于裁剪且在切割过程中不会碎裂。它们还有低背景,广泛溶剂相容性以及优异的着色能力。此外,它们可以重复检测。
PVDF膜即聚偏二氟乙烯膜(polyvinylidene fluoride)是蛋白质印迹法中常用的一种固相支持物。PVDF膜是疏水性的,膜孔径有大有小,随着膜孔径的不断减小,膜对低分子量的蛋白结合就越牢固。大于20000的蛋白选用0.45um的膜,小于20000的蛋白选用0.2um的膜。PVDF膜在使用时需预处理,用甲醇处理的目的是活化膜上的正电基团,使其更容易与带负电的蛋白结合。PVDF膜具有较高的机械强度,是印迹法中的理想固相支持物材料。
Millipore密理博PVDF膜 0.45um孔径 转印膜 IPVH00010
标签归档:ipvh
IPVH00010 ISEQ00010-Millipore NC膜硝酸纤维素膜PVDF膜WB实验膜
产品型号IPVH00010 ISEQ00010
品 牌密理博
厂商性质代理商
Millipore NC膜硝酸纤维素膜PVDF膜WB实验膜,无需封闭,采用 Millipore 快速免疫检测方法,可以省去蛋白质印迹中的封闭步骤,为您节省2小时的检测时间特点:背景低,灵敏度高,操作性强,着色更容易,结果更出色.
详情介绍
Protein Blotting&Sequencing
请使用 0.45um 疏水性 PVDF( 聚偏二氟乙烯 ) 膜 Immobilon-P
Immobilon-P转印膜是用来吸附从各种凝胶中转印的蛋白质的性价比高的膜.这种疏水性膜呈均一控制结构,具有*的吸附生物分子的能力.与硝酸纤维膜相比,Immobilon-P具有易于操作,染色性能好,抗溶剂能力强和信噪比高的特点,提高了检测的灵敏度.其开放的孔结构使其容易接近被吸附的蛋白质,并去除背景上没有吸附的探针,因而成为免疫检测的印迹膜.如果采用Millipore快速免疫检测方法,研究人员可以缩短检测时间2小时.Immobilon-P转印膜的疏水性不仅可以省去蛋白质印迹中的封闭步骤,而且还可以显著地降低所需的洗脱次数和时间,且特异性和灵敏度不受任何影响.
Protein Blotting&Sequencing, 小分子蛋白质 (<20kDa)
请使用 0.22um 疏水性 PVDF( 聚偏二氟乙烯 ) 膜 Immobilon-PSQ
Immobilon-PSQ转印膜是印迹小分子蛋白质(<20kDa)的理想选择.与其它转印膜相比较,该膜具有优良的蛋白质吸附性能,渗漏甚少.其内表面面积大,适合蛋白质吸附,从而提高蛋白质测序的得率.
Immobilon-PSQ转印膜不含污染性支撑物或表面活性剂,因而信号强而背景值极小.
做杂交还可使用价格更实惠的硝酸纤维素膜 Immobilon-NC | 详细请咨询 | |||||||||||||||||||
订购信息:
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millipore转印膜IPVH08130 IPVH08130
millipore转印膜IPVH08130
millipore转印膜IPVH08130 Millipore 提供四种不同的不动 PVDF 膜,每种膜都针对不同的蛋白质印迹应用进行优化。 millipore转印膜IPVH08130 孔径0.45 微米
millipore转印膜IPVH08130
Millipore 提供三种不同的Immobilon PVDF膜,是针对不同的蛋白质印迹应用的理想选择。现在我们还提供采用按规格裁切的印迹方片膜和两层过滤纸组成的印迹三明治。
与硝酸纤维素膜相比,Immobilon膜具有多种优点,它们不易破裂或卷曲,便于裁剪且在切割过程中不会碎裂。它们还有低背景,广泛溶剂相容性以及优异的着色能力。此外,它们可以重复检测。
Description | |
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Catalogue Number | IPVH08130 |
Trade Name |
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Description | Immobilon-P PVDF Membrane |
Background Information | Overview – Millipore offers four different Immobilon PVDF membranes, each optimized for different protein blotting applications. Immobilon PVDF membranes provide many advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times. Immobilon-P Transfer Membrane: – 45 µm pore size Recommended for most western blotting, especially proteins greater than 20 kDa – Millipore’s Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity Immobilon-E Membrane: – the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step. – 45 µm pore size recommended for most western blotting, especially proteins greater than 20 kDa Blotting Sandwiches: – When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper and are compatible with any standard wet transfer device. Blotting Filter Paper: – Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes. Immobilon-PSQ Membrane: – 0.2 µm pore size and a large internal structure – Higher protein adsorption and sequencing yields than other membranes – Recommended for blotting proteins less than 20 kDa – Prevents blow-through of low molecular weight proteins Immobilon-FL Membrane: – The first transfer membrane optimized for fluorescence applications – Extremely low background improves sensitivity of all fluorescence detection protocols – Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths – Ideal for multiplexing and chemifluorescence applications Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon® PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. In addition, Immobilon® PVDF membranes optimized for fluorescent blots dramatically increase signal-to-noise ratios for high sensitivity in quantitative, multiplexing applications. Features & Benefits: – Won’t crack, curl or fracture when cut – Low background – Superior staining capabilities – Can be reprobed multiple times Applications: – Western Blotting, Immunoblotting, Dot Blotting, Protein Sequencing; – Compatible with Chemiluminescent, Fluorescent, Radioactive, Chromogenic, and Chemifluorescent Detection – Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths |
References |
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Product Information | |
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Detection method | Chromogenic Radioactive Fluorescent Chemiluminescence |
Compatible Stains |
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Filter Code | IPVH |
Filter Color | White |
Applications | |
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Application | 10 sheets, 8.5 cm x 13.5 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting. |
Key Applications |
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Biological Information | |
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Primary Binding Mechanisms | Electrostatic, hydrophobic |
BSA Adsorption | 215 µg/cm² |
Goat IgG Adsorption | 294 µg/cm² |
Insulin Adsorption | 160 µg/cm² |
Wettability | Hydrophobic |
Physicochemical Information | |
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Pore Size | 0.45 µm |
Dimensions | |
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Filter Surface | Plain |
Filter Dimensions | 8.5 cm x 13.5 cm |
Materials Information | |
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Chemistry |
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Toxicological Information |
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Safety Information according to GHS |
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Safety Information |
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Product Usage Statements |
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Storage and Shipping Information |
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Packaging Information | |
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Material Size | 10 |
Millipore密理博 Immobilon转印膜IPVH00010 ipvh00010
Millipore密理博 Immobilon转印膜IPVH00010
Millipore密理博 Immobilon转印膜IPVH00010 规格:孔径0.45μm PVDF膜 尺寸:26.5cm X 3.75m Millipore密理博 Immobilon转印膜IPVH00010
Millipore密理博 mmobilon 转印膜 IPVH00010
尺寸 |
|
过滤器表面 |
光面 |
过滤器尺寸 |
26.5cm x 3.75m 孔径;0.45μm
|
材料信息 |
|
化学 |
Hydrophobic PVDF |
生物信息 |
|
主结合机制 |
静电,疏水 |
BSA吸收性(µg/cm²) |
215 |
山羊IgG吸收性(µg/cm²) |
294 |
胰岛素吸收性(µg/cm²) |
160 |
可湿润性 |
疏水 |
Millipore 提供三种不同的Immobilon PVDF膜,是针对不同的蛋白质印迹应用的理想选择。现在我们还提供采用按规格裁切的印迹方片膜和两层过滤纸组成的印迹三明治。
与硝酸纤维素膜相比,Immobilon膜具有多种优点,它们不易破裂或卷曲,便于裁剪且在切割过程中不会碎裂。它们还有低背景,广泛溶剂相容性以及优异的着色能力。此外,它们可以重复检测。
PVDF膜即聚偏二氟乙烯膜(polyvinylidene fluoride)是蛋白质印迹法中常用的一种固相支持物。PVDF膜是疏水性的,膜孔径有大有小,随着膜孔径的不断减小,膜对低分子量的蛋白结合就越牢固。大于20000的蛋白选用0.45um的膜,小于20000的蛋白选用0.2um的膜。PVDF膜在使用时需预处理,用甲醇处理的目的是活化膜上的正电基团,使其更容易与带负电的蛋白结合。PVDF膜具有较高的机械强度,是印迹法中的理想固相支持物材料。
|
milliporePVDF膜 IPVH00010 IPVH00010
milliporePVDF膜 IPVH00010
milliporePVDF膜 IPVH00010 应用于Western blotting、吸附分析、氨基酸分析、N 端蛋白质测序、Dot/Slot blotting、糖蛋白显色和脂多糖分析 milliporePVDF膜 IPVH00010 Immobilon-P 卷膜,PVDF,0.45 µm,26.5 cm x 3.75 m
milliporePVDF膜 IPVH00010
主结合机制 |
静电,疏水 |
BSA吸收性(µg/cm²) |
215 |
山羊IgG吸收性(µg/cm²) |
294 |
胰岛素吸收性(µg/cm²) |
160 |
可湿润性 |
疏水 |
孔径(µm) |
0.45 |
过滤器表面 |
光面 |
过滤器尺寸(cm x cm) |
26.5 x 375 |