标签归档：jf

荧光染料Janelia Fluor® 526, SE(Synonyms: JF526, SE; JF526, NHS)

发表于由

荧光染料Dye Reagents Janelia Fluor® 526, SE;(Synonyms: JF526, SE; JF526, NHS)

Janelia Fluor® 526, SE (JF526,SE) 是一种含有 NHS 酯基的荧光黄色荧光染料。JF526 是一种用于荧光配体的多功能支架，包括用于遗传编码的自标记蛋白质标签和用于内源性结构的染色。Janelia Fluor® 产品在美国专利下获得许可，来自 Howard Hughes Medical Institute 的 No.9,933,417，10,018,624，10,161,932 和其他专利。

Janelia Fluor® 526, SE(Synonyms: JF526, SE; JF526, NHS)

Janelia Fluor® 526, SE Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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生物活性

Janelia Fluor® 526, SE (JF526,SE) is a fluorogenic yellow fluorescent dye that contains NHS ester group. JF526 is a versatile scaffold for fluorogenic ligands, including labels for genetically encoded self-labeling protein tags and stains for endogenous structures[1]. Janelia Fluor® products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.

体外研究
(In Vitro)

Excitation maximum=526 nm; emission maximum=550 nm[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

659.49

Formula

C31H19F6N3O7
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Zheng Q, et al. Rational Design of Fluorogenic and Spontaneously Blinking Labels for Super-Resolution Imaging [published correction appears in ACS Cent Sci. 2020 Oct 28;6(10):1844]. ACS Cent Sci. 2019;5(9):1602-1613.

荧光染料Janelia Fluor® 669, SE(Synonyms: JF669, SE; JF669, NHS)

发表于由

荧光染料Dye Reagents Janelia Fluor® 669, SE;(Synonyms: JF669, SE; JF669, NHS)

Janelia Fluor® 669, SE (JF669, SE) 是一种红色的荧光染料，可与含巯基的卤代标记配体在温和的条件下 (DIEA, DMF) 反应，一步形成 JF669-HaloTag (Ex = 669 nm; Em = 682 nm)。Janelia Fluor® 产品在美国专利下获得许可，来自 Howard Hughes Medical Institute 的 No.9,933,417，10,018,624，10,161,932 和其他专利。

Janelia Fluor® 669, SE(Synonyms: JF669, SE; JF669, NHS)

Janelia Fluor® 669, SE Chemical Structure

CAS No. : 2127150-20-1

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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生物活性

Janelia Fluor® 669, SE (JF669, SE), a red fluorescent dye, can be directly reacted with the available thiol-containing HaloTag ligand under mild conditions (DIEA, DMF) to afford a JF669−HaloTag ligand in a single step (Ex = 669 nm; Em = 682 nm)[1]. Janelia Fluor® products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.

体外研究
(In Vitro)

JF669 undergoes rapid conversion to thioether product (<5 min to completion) in organic solvents, where the dye predominantly adopts closed form[1].
JF669 displays comparatively excellent photostability, retaining 97% fluorescence after the same number of cycles[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

693.76

Formula

C34H30F3N3O6SSi
CAS 号

2127150-20-1
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Jonathan B Grimm, et al. General Synthetic Method for Si-Fluoresceins and Si-Rhodamines. ACS Cent Sci. 2017 Sep 27;3(9):975-985.

荧光染料PA Janelia Fluor® 549, SE(Synonyms: PA-JF549-NHS)

发表于由

荧光染料Dye Reagents PA Janelia Fluor® 549, SE;(Synonyms: PA-JF549-NHS)

PA Janelia Fluor® 549, SE 是光激活的探针 (JF549,SE (JF549,NHS) 衍生物)。JF549,SE (JF549,NHS) 是一种荧光染料，最大吸收波长 (λab (max)) 为 549 nm，最大发射波长 (λem (max)) 为 571 nm。Janelia Fluor® 产品在美国专利下获得许可，来自 Howard Hughes Medical Institute 的 No.9,933,417，10,018,624，10,161,932 和其他专利。

PA Janelia Fluor® 549, SE(Synonyms: PA-JF549-NHS)

PA Janelia Fluor® 549, SE Chemical Structure

CAS No. : 1811539-42-0

规格 是否有货
50 μg 询价

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生物活性

PA Janelia Fluor® 549, SE (PA-JF549-NHS) is a bright photoactivatable fluorophore of JF549,SE (JF549,NHS). JF549,SE (JF549,NHS) is a fluorescent dye with the absorption maximum (λab (max)) of 549 nm and emission maximum (λem (max)) of 571 nm[1][2]. Janelia Fluor® products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.

分子量

575.57

Formula

C32H25N5O6
CAS 号

1811539-42-0
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Jonathan B Grimm, et al. Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and Their Use in Cellular Imaging Experiments. Methods Mol Biol. 2017;1663:179-188.

    [2]. Jonathan B Grimm, et al. Bright photoactivatable fluorophores for single-molecule imaging. Nat Methods. 2016 Dec;13(12):985-988.