荧光染料DiAzKs(Synonyms: H-L-Photo-lysine)

荧光染料Dye Reagents DiAzKs;(Synonyms: H-L-Photo-lysine) 纯度: ge;98.0%

DiAzKs (H-L-Photo-lysine) 是一种含有重氮基的赖氨酸氨基酸,也是一种光交联剂。DiAzKs 可以选择性的插入到蛋白质中,并用于在体外和活细胞中交联蛋白质-蛋白质相互作用。DiAzKs 用作紫外光激活的光交联探针。

DiAzKs(Synonyms: H-L-Photo-lysine)

DiAzKs Chemical Structure

CAS No. : 1253643-88-7

规格 价格 是否有货 数量
5 mg ¥1650 In-stock
10 mg ¥2600 In-stock
50 mg ¥6500 In-stock
100 mg ; 询价 ;
200 mg ; 询价 ;

* Please select Quantity before adding items.

DiAzKs 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

DiAzKs (H-L-Photo-lysine) is a diazirine-containing lysine amino acid and is a photo-cross-linker. DiAzKs can site-selective incorporated into proteins and is used to crosslink protein-protein interactions in vitro and in living cells. DiAzKs acts as a UV light-activated photo-crosslinking probe[1][2][3].

体外研究
(In Vitro)

Photo-lysine, which is readily incorporated into proteins by native mammalian translation machinery, can be used to capture and identify proteins that recognize lysine post-translational modifications (PTMs), including ‘readers’ and ‘erasers’ of histone modifications[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

272.30

Formula

C11H20N4O4

CAS 号

1253643-88-7

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20deg;C 3 years
4deg;C 2 years
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

H2O : 5 mg/mL (18.36 mM; Need ultrasonic)

DMSO : 2 mg/mL (7.34 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.6724 mL 18.3621 mL 36.7242 mL
5 mM 0.7345 mL 3.6724 mL 7.3448 mL
10 mM 0.3672 mL 1.8362 mL 3.6724 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 0.67 mg/mL (2.46 mM); Clear solution

    此方案可获得 ≥ 0.67 mg/mL (2.46 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 6.7 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 0.67 mg/mL (2.46 mM); Clear solution

    此方案可获得 ≥ 0.67 mg/mL (2.46 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 6.7 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Ai HW, et al. Probing protein-protein interactions with a genetically encoded photo-crosslinking amino acid. Chembiochem. 2011 Aug 16;12(12):1854-7.

    [2]. Chatterjee A, et al. Efficient viral delivery system for unnatural amino acid mutagenesis in mammalian cells. Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):11803-8.

    [3]. Yang T, et al. Photo-lysine captures proteins that bind lysine post-translational modifications. Nat Chem Biol. 2016 Feb;12(2):70-2.

Photo-lysine hydrochloride

生化分析试剂 Biochemical Assay Reagents
Photo-lysine hydrochloride;

Photo-lysine hydrochloride,一种新的基于赖氨酸光反应性的氨基酸,它捕获结合赖氨酸翻译后修饰的蛋白质。

Photo-lysine hydrochloride

Photo-lysine hydrochloride Chemical Structure

规格 价格 是否有货 数量
5 mg ¥12000 In-stock
10 mg ¥21600 In-stock
20 mg ¥36000 询价
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

Photo-lysine hydrochloride, a new lysine-based photo-reactive amino acid, captures proteins that bind lysine post-translational modifications.

体外研究
(In Vitro)

Photo-lysine is designed and synthesized by incorporating a photo-cross-linker (diazirine) into the side chain of natural lysine. Photo-lysine, which is readily incorporated into proteins by native mammalian translation machinery, can be used to capture and identify proteins that recognize lysine post-translational modifications (PTMs), including ‘readers’ and ‘erasers’ of histone modifications. Photo-lysine can be incorporated into MDH2 and mediate photo-cross-linking to fix protein-protein interactions in cells. UV irradiation of cells in the presence of photo-lysine induced robust cross-linking of HSP90β and HSP60. Photo-lysine has higher efficiency than photo-leucine for photo-cross-linking of the two chaperone proteins. Photo-lysine enables capture of the heterodimer of proteins Ku70 and Ku80 within a protein complex. Photo-lysine enables identification of histone- and chromatin-binding proteins[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

245.11

Formula

C6H14Cl2N4O2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, protect from light, stored under nitrogen

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light, stored under nitrogen)

溶解性数据
In Vitro:;

H2O : ≥ 40 mg/mL (163.19 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 4.0798 mL 20.3990 mL 40.7980 mL
5 mM 0.8160 mL 4.0798 mL 8.1596 mL
10 mM 0.4080 mL 2.0399 mL 4.0798 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Yang T, et al. Photo-lysine captures proteins that bind lysine post-translational modifications. Nat Chem Biol. 2016 Feb;12(2):70-2.

Photo-lysine(Synonyms: Photo lysine)

生化分析试剂 Biochemical Assay Reagents
Photo-lysine;(Synonyms: Photo lysine)

Photo-lysine,一种新的基于赖氨酸光反应性的氨基酸,它捕获结合赖氨酸翻译后修饰的蛋白质。

Photo-lysine(Synonyms: Photo lysine)

Photo-lysine Chemical Structure

CAS No. : 1863117-91-2

规格 价格 是否有货
5 mg ¥12000 询问价格 货期
10 mg ¥21600 询问价格 货期
20 mg ¥36000 询问价格 货期

* Please select Quantity before adding items.

Photo-lysine 的其他形式现货产品:

Photo-lysine hydrochloride

生物活性

Photo-lysine, a new lysine-based photo-reactive amino acid, captures proteins that bind lysine post-translational modifications.

体外研究
(In Vitro)

Photo-lysine is designed and synthesized by incorporating a photo-cross-linker (diazirine) into the side chain of natural lysine. Photo-lysine, which is readily incorporated into proteins by native mammalian translation machinery, can be used to capture and identify proteins that recognize lysine post-translational modifications (PTMs), including ‘readers’ and ‘erasers’ of histone modifications. Photo-lysine can be incorporated into MDH2 and mediate photo-cross-linking to fix protein-protein interactions in cells. UV irradiation of cells in the presence of photo-lysine induced robust cross-linking of HSP90β and HSP60. Photo-lysine has higher efficiency than photo-leucine for photo-cross-linking of the two chaperone proteins. Photo-lysine enables capture of the heterodimer of proteins Ku70 and Ku80 within a protein complex. Photo-lysine enables identification of histone- and chromatin-binding proteins[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

172.19

Formula

C6H12N4O2

CAS 号

1863117-91-2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Yang T, et al. Photo-lysine captures proteins that bind lysine post-translational modifications. Nat Chem Biol. 2016 Feb;12(2):70-2.

Pall Lysine HyperD 亲和填料, 20059-058, 20059-036, 20059-028, 20059-010, 20059-044

名称:Lysine HyperD 亲和填料

型号:20059-058, 20059-036, 20059-028, 20059-010, 20059-044

简介:Pall 颇尔 Lysine HyperD 亲和填料, 20059-058, 20059-036, 20059-028, 20059-010, 20059-044

Pall 颇尔 Lysine HyperD 亲和填料, 20059-058, 20059-036, 20059-028, 20059-010, 20059-044

 
说明
·      Pall Lysine HyperD 亲和填料, 用于高流速/高载量的纯化与赖氨酸结合的蛋白(生物分子)。
·      集合了软性胶/高流速和高载量优势的刚性填料。
·      软性胶、高流速在压力的增加的情况下不会皱缩和变形。
·      快速填装、填料的高密度允许了快速填装。
·      能填装于各种柱规格。
Pall Lysine HyperD 亲和填料, 20059-058, 20059-036, 20059-028, 20059-010, 20059-044
Pall 颇尔 Lysine HyperD 亲和填料, 20059-058, 20059-036, 20059-028, 20059-010, 20059-044
 
应用
·      – 纯化与凝血作用相关的蛋白质,如血浆酶原和血浆酶原激活蛋白。
·      – 纯化来自人血浆的细胞色素C氧化酶和纤维蛋白原。
 
Pall 颇尔 Lysine HyperD 亲和填料, 20059-058, 20059-036, 20059-028, 20059-010, 20059-044
 
订购信息
货号
描述
包装
20059-058
Pall Lysine HyperD 亲和填料
5 mL
20059-036
 
25 mL
20059-028
 
100 mL
20059-010
 
1000 mL
20059-044
 
10000 mL
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Pall 颇尔 Lysine HyperD 亲和填料, 20059-058, 20059-036, 20059-028, 20059-010, 20059-044