Malantide

Malantide; 纯度: 98.56%

Malantide 是一种合成的十二肽,由被磷酸化酶激酶β亚基上的 PKA 磷酸化的位点衍生而来。Malantide 是 PKA 的高度特异性底物,Km 为 15 μM,并且在各种大鼠组织提取物中显示出 PKI 抑制 > 90%底物磷酸化。Malantide 也是 PKC 的有效底物,Km 为 16 μM。

Malantideamp;;

Malantide Chemical Structure

CAS No. : 86555-35-3

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5 mg ¥1800 In-stock
10 mg ¥2800 In-stock
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  • Peptide Library

生物活性

Malantide is a synthetic dodecapeptide derived from the site phosphorylated by cAMP-dependent protein kinase (PKA) on the β-subunit of phosphorylase kinase. Malantide is a highly specific substrate for PKA with a Km of 15 μM and shows protein inhibitor (PKI) inhibition >90% substrate phosphorylation in various rat tissue extracts[1]. Malantide is also an efficient substrate for PKC with a Km of 16 μM[2].

IC50 Target

Km: 15 μM (PKA)[1], 16 μM (PKC)[2], 233 μM (PKG)[1]

体外研究
(In Vitro)

The Km values of Malantide are 15 μM and 223 μM for PKA and PKG, respectively. The Vmax values are 23.8 units/mg and 6.6 units/mg for for PKA and PKG, respectively[1].
A statistically significant effect of isoprenaline on the activity ratio of guinea-pig heart was only: observed with Malantide[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1633.89

Formula

C72H124N22O21

CAS 号

86555-35-3

Sequence

Arg-Thr-Lys-Arg-Ser-Gly-Ser-Val-Tyr-Glu-Pro-Leu-Lys-Ile

Sequence Shortening

RTKRSGSVYEPLKI

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献
  • [1]. Murray KJ, et al. Use of a synthetic dodecapeptide (malantide) to measure the cyclic AMP-dependent protein kinase activity ratio in a variety of tissues. Biochem J. 1990 May 1;267(3):703-8.

    [2]. Z H Zhao, et al. Characterization of a New Substrate for Protein Kinase C: Assay by Continuous Fluorometric Monitoring and High Performance Liquid Chromatography. Biochem Biophys Res Commun. 1991 May 15;176(3):1454-61.

Malantide TFA

Malantide TFA;

Malantide TFA 是一种合成的十二肽,由被磷酸化酶激酶β亚基上的 PKA 磷酸化的位点衍生而来。Malantide TFA 是 PKA 的高度特异性底物,Km 为 15 μM,并且在各种大鼠组织提取物中显示出 PKI 抑制 > 90%底物磷酸化。Malantide TFA 也是 PKC 的有效底物,Km 为 16 μM。

Malantide TFAamp;;

Malantide TFA Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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Malantide TFA 的其他形式现货产品:

Malantide

生物活性

Malantide TFA is a synthetic dodecapeptide derived from the site phosphorylated by cAMP-dependent protein kinase (PKA) on the β-subunit of phosphorylase kinase. Malantide TFA is a highly specific substrate for PKA with a Km of 15 μM and shows protein inhibitor (PKI) inhibition >90% substrate phosphorylation in various rat tissue extracts[1]. Malantide TFA is also an efficient substrate for PKC with a Km of 16 μM[2].

IC50 Target

Km: 15 μM (PKA)[1], 16 μM (PKC)[2], 233 μM (PKG)[1]

体外研究
(In Vitro)

The Km values of Malantide are 15 μM and 223 μM for PKA and PKG, respectively. The Vmax values are 23.8 units/mg and 6.6 units/mg for for PKA and PKG, respectively[1].
A statistically significant effect of isoprenaline on the activity ratio of guinea-pig heart was only: observed with Malantide[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1747.91

Formula

C74H125F3N22O23

Sequence

Arg-Thr-Lys-Arg-Ser-Gly-Ser-Val-Tyr-Glu-Pro-Leu-Lys-Ile

Sequence Shortening

RTKRSGSVYEPLKI

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. K J Murray, et al. Use of a Synthetic Dodecapeptide (Malantide) to Measure the Cyclic AMP-dependent Protein Kinase Activity Ratio in a Variety of Tissues. Biochem J. 1990 May 1;267(3):703-8.

    [2]. Z H Zhao, et al. Characterization of a New Substrate for Protein Kinase C: Assay by Continuous Fluorometric Monitoring and High Performance Liquid Chromatography. Biochem Biophys Res Commun. 1991 May 15;176(3):1454-61.