标签归档：mating

α-Factor Mating Pheromone, yeast TFA(Synonyms: Mating Factor α TFA)

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α-Factor Mating Pheromone, yeast TFA;(Synonyms: Mating Factor α TFA)

α-Factor Mating Pheromone, yeast (TFA) 是一种十三肽，由 S. cerevisiae 的 α 细胞分泌产生，通过 Ste2p 受体起作用。

α-Factor Mating Pheromone, yeast TFAamp;;(Synonyms: Mating Factor α TFA)

α-Factor Mating Pheromone, yeast TFA Chemical Structure

规格 价格 是否有货 数量
1 mg ￥800 In-stock
5 mg ￥2800 In-stock
10 mg ￥4800 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

α-Factor Mating Pheromone, yeast (TFA) is a tridecapeptide secreted by S. cerevisiae α cells via Ste2p receptor[1].

分子量

1798.02

Formula

C84H115N20F3O19S
Sequence

Trp-His-Trp-Leu-Gln-Leu-Lys-Pro-Gly-Gln-Pro-Met-Tyr
Sequence Shortening

WHWLQLKPGQPMY
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture and light

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. [1]. Naider F, et al. The alpha-factor mating pheromone of Saccharomyces cerevisiae: a model for studying the interaction of peptide hormones and G protein-coupled receptors. Peptides. 2004 Sep;25(9):1441-63.