标签归档：mmp

供应MMP13 Goat anti-Mouse Polyclonal Antibody – LS-C8860

发表于由

基质金属蛋白酶13（MMP13）是人体最重要的蛋白酶之一，其主要功能是分解细胞外基质，与乳腺癌、骨关节炎和风湿性关节炎等病理过程有关。临床应用发现广谱基质金属蛋白酶抑制剂具有严重的肌肉和骨骼副作用。所以现在研究主要集中在有选择性的MMP13抑制剂的研究。

Lifespan公司的LS-C8860产品特异性识别大鼠和小鼠的MMP-13, pro-MMP-13, and most autolytic fragments of MMP-13. 与human MMP-13, mouse MMP-2, MMP-3 or MMP-9没有交叉反应.该产品可应用于免疫组化冰冻切片，免疫细胞化学，WB，IP等应用。

Lifespan公司产品致力于免疫组化方面的应用，质量100%保证，保证了客户不会因为产品问题浪费实验时间，快速得出准确实验结果。

lifespan，美国品牌，原装进口，致力于生产和开发各类抗体，蛋白及相关的试剂盒。如果您对上述产品感兴趣，请致电021-50837765,021-63599871/63599872到上海金畔生物科技有限公司垂询细胞研究的相关实验解决方案，或索取最新的lifespan产品资料。

CTTHWGFTLC, CYCLIC

发表于由

CTTHWGFTLC, CYCLIC;

CTTHWGFTLC, CYCLIC 是一种基质金属蛋白酶 MMP-2 和 MMP-9 的环肽抑制剂。对 MMP-9 的 IC50 约为 8 μM。

CTTHWGFTLC, CYCLICamp;;

CTTHWGFTLC, CYCLIC Chemical Structure

CAS No. : 244082-19-7

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

CTTHWGFTLC, CYCLIC is a cyclic peptide inhibitor for matrix metalloproteinases MMP-2 and MMP-9. The IC50 value for MMP-9 is ~8 μM[1].

IC50 Target

MMP-9

;

MMP-2

;

体外研究
(In Vitro)

CTTHWGFTLC, CYCLIC inhibits endothelial and tumor cell migration in vitro[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究
(In Vivo)

CTTHWGFTLC, CYCLIC inhibits tumor progression in vivo, in mouse models[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1166.33

Formula

C52H71N13O14S2
CAS 号

244082-19-7
Sequence

Cys-Thr-Thr-His-Trp-Gly-Phe-Thr-Leu-Cys (Disulfide Bridge: Cys1-Cys10)
Sequence Shortening

CTTHWGFTLC (Disulfide Bridge: Cys1-Cys10)
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Medina OP, et al. Binding of novel peptide inhibitors of type IV collagenases to phospholipid membranes and use in liposome targeting to tumor cells in vitro. Cancer Res. 2001 May 15;61(10):3978-85.

GPLGIAGQ

发表于由

GPLGIAGQ;

GPLGIAGQ 是一种 MMP2 可降解 (cleavable) 的多肽，在脂质体和胶束纳米载体中都被用作刺激敏感的连接物，用于 MMP2 触发的肿瘤靶向研究。GPLGIAGQ 可用于合成光动力疗法 (PDT) 中独特的MMP2靶向光敏剂。

GPLGIAGQamp;;

GPLGIAGQ Chemical Structure

CAS No. : 109053-09-0

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

GPLGIAGQ 的其他形式现货产品:

GPLGIAGQ TFA

生物活性

GPLGIAGQ, a MMP2-cleavable polypeptide, is used as a stimulus-sensitive linker in both liposomal and micellar nanocarriers for MMP2-triggered tumor targeting. GPLGIAGQ can be used to synthesis unique MMP2-targeted photosensitizer in photodynamic therapy (PDT)[1][2][3].

IC50 Target

MMP2[1].
体外研究
(In Vitro)

GPLGIAGQ is used to trigger PEG deshielding of liposomal carriers, resulting in enhanced cellular internalization[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

711.81

Formula

C31H53N9O10
CAS 号

109053-09-0
Sequence

Gly-Pro-Leu-Gly-Ile-Ala-Gly-Gln
Sequence Shortening

GPLGIAGQ
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Hou W, et al. MMP2-Targeting and Redox-Responsive PEGylated Chlorin e6 Nanoparticles for Cancer Near-Infrared Imaging and Photodynamic Therapy. ACS Appl Mater Interfaces. 2016 Jan 20;8(2):1447-57.

    [2]. Zhu L, et al. Matrix metalloproteinase 2-sensitive multifunctional polymeric micelles for tumor-specific co-delivery of siRNA and hydrophobic drugs. Biomaterials. 2014 Apr;35(13):4213-22.

    [3]. Liu FH, et al. Enzyme-sensitive cytotoxic peptide-dendrimer conjugates enhance cell apoptosis and deep tumor penetration. Biomater Sci. 2018 Feb 27;6(3):604-613.

FFAGLDD

发表于由

FFAGLDD;

FFAGLDD 是 MMP9 选择性切割肽，用于阿霉素 (DOX) 的胞质递送，并实现了时间和空间控制的缓慢药物递送和释放。

FFAGLDDamp;;

FFAGLDD Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

FFAGLDD is MMP9 selective cleavage peptides, which used for cytosolic delivery of Doxorubi-cin (DOX) and achieve temporally and spatially controlled slow drug delivery and release[1].

IC50 Target[1]

MMP9

;

分子量

783.82

Formula

C37H49N7O12
Sequence Shortening

FFAGLDD
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Zhou Q, et al. Stimuli-responsive polymeric micelles for drug delivery and cancer therapy. Int J Nanomedicine. 2018 May 18;13:2921-2942.

Apigenin-7-glucuronide(Synonyms: Apigenin 7-O-glucuronide)

发表于由

天然产物 黄酮类 Flavonoids

Apigenin-7-glucuronide (Synonyms: Apigenin 7-O-glucuronide) 纯度: 98.83%

Apigenin-7-glucuronide 能够抑制基质金属蛋白酶 (MMP) 的活性，其对 MMP-3，MMP-8，MMP-9，MMP-13 的IC50 值分别为 12.87，22.39，17.52，0.27 μM。

Apigenin-7-glucuronide(Synonyms: Apigenin 7-O-glucuronide)

Apigenin-7-glucuronide Chemical Structure

CAS No. : 29741-09-1

规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥1650 In-stock
5 mg ￥1500 In-stock
10 mg ￥2500 In-stock
25 mg ￥4500 In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

Apigenin-7-glucuronide 相关产品

•相关化合物库:

  • Natural Product Library Plus
  • Bioactive Compound Library Plus
  • Metabolism/Protease Compound Library
  • Natural Product Library
  • Anti-Cancer Compound Library
  • Differentiation Inducing Compound Library
  • Glycoside Compound Library
  • Phenols Library
  • Flavonoids Library
  • Anti-Pancreatic Cancer Compound Library
  • Angiogenesis Related Compound Library
  • Food-Sourced Compound Library

生物活性

Apigenin-7-glucuronide could inhibit Matrix Metalloproteinases (MMP) activities, with IC50s of 12.87, 22.39, 17.52, 0.27 μM for MMP-3, MMP-8, MMP-9, MMP-13, respectively.

IC50 & Target

MMP-3

12.87 μM (IC50)

MMP-8

22.39 μM (IC50)

MMP-9

17.52 μM (IC50)

MMP13

0.27 μM (IC50)

体外研究
(In Vitro)

Apigenin-7-Oglucuronide could inhibit Matrix Metalloproteinases (MMP) activities, with IC50s of 12.87, 22.39, 17.52, 0.27 μM for MMP-3, MMP-8, MMP-9, MMP-13, respectively [1]. Scutellarin A also inhibits the migrated capacity of ACHN and 786-O cells in a dose-dependent manner. It is also revealed that after treatment with Scutellarin A (30, 60, and 90 μM) for 24 h, the apoptosis rates in ACHN and 786-O cells are remarkably enhanced dose-dependently when compared to the control groups[2].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

446.36

Formula

C21H18O11
CAS 号

29741-09-1
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO : 250 mg/mL (560.09 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.2403 mL 11.2017 mL 22.4034 mL
5 mM 0.4481 mL 2.2403 mL 4.4807 mL
10 mM 0.2240 mL 1.1202 mL 2.2403 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂： 10% DMSO    90% corn oil

    Solubility: ≥ 2.17 mg/mL (4.86 mM); Clear solution

    此方案可获得 ≥ 2.17 mg/mL (4.86 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 21.7 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

  • 2.

    请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (4.66 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (4.66 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 3.

    请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (4.66 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (4.66 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
*以上所有助溶剂都可在 Shanghai Jinpan Biotech Co Ltd 网站选购。
参考文献

  • [1]. Crascì L, et al. Correlating In Vitro Target-Oriented Screening and Docking: Inhibition of Matrix Metalloproteinases Activities by Flavonoids. Planta Med. 2017 Jul;83(11):901-911.

    [2]. Deng W, et al. Scutellarin inhibits human renal cancer cell proliferation and migration via upregulation of PTEN. Biomed Pharmacother. 2018 Nov;107:1505-1513.

Luteolin 7-O-glucuronide(Synonyms: Luteolin 7-glucuronide)

发表于由

天然产物 糖类和糖苷 Saccharides and Glycosides

Luteolin 7-O-glucuronide;(Synonyms: Luteolin 7-glucuronide) 纯度: 99.80%

Luteolin 7-O-glucuronide 可作为有效的自由基清除剂，能够抑制基质金属蛋白酶 (MMP) 的活性，其对 MMP-1，MMP-3，MMP-8，MMP-9，MMP-13的IC50 值分别为 17.63，7.99，11.42，12.85，0.03 μM。

Luteolin 7-O-glucuronide(Synonyms: Luteolin 7-glucuronide)

Luteolin 7-O-glucuronide Chemical Structure

CAS No. : 29741-10-4

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ￥1210 In-stock
5 mg ￥1100 In-stock
10 mg ￥1800 In-stock
25 mg ￥3900 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Luteolin 7-O-glucuronide 相关产品

bull;相关化合物库:

  • Natural Product Library Plus
  • Bioactive Compound Library Plus
  • Metabolism/Protease Compound Library
  • Natural Product Library
  • Differentiation Inducing Compound Library
  • Glycoside Compound Library
  • Phenols Library
  • Traditional Chinese Medicine Monomer Library
  • Flavonoids Library
  • Anti-Pancreatic Cancer Compound Library
  • Angiogenesis Related Compound Library
  • Food-Sourced Compound Library
  • Targeted Diversity Library

生物活性

Luteolin 7-O-glucuronide could inhibit Matrix Metalloproteinases (MMP) activities, with IC50s of 17.63, 7.99, 11.42, 12.85, 0.03 μM for MMP-1, MMP-3, MMP-8, MMP-9, MMP-13, respectively.

IC50 Target

MMP-1

17.63 mu;M (IC50)

MMP-3

7.99 mu;M (IC50)

MMP-8

11.42 mu;M (IC50)

MMP-9

12.85 mu;M (IC50)

MMP13

0.03 mu;M (IC50)

分子量

462.36

Formula

C21H18O12
CAS 号

29741-10-4
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)
溶解性数据
In Vitro:;

DMSO : 125 mg/mL (270.35 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.1628 mL 10.8141 mL 21.6282 mL
5 mM 0.4326 mL 2.1628 mL 4.3256 mL
10 mM 0.2163 mL 1.0814 mL 2.1628 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;0.5% MC;;0.5% Tween-80

    Solubility: 15.71 mg/mL (33.98 mM); Suspended solution; Need ultrasonic

  • 2.

    请依序添加每种溶剂：;50% PEG300 ;; 50% saline

    Solubility: 5 mg/mL (10.81 mM); Clear solution; Need ultrasonic

  • 3.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.08 mg/mL (4.50 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (4.50 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 4.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (4.50 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (4.50 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
  • 5.

    请依序添加每种溶剂：;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.08 mg/mL (4.50 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (4.50 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Crascì L, et al. Correlating In Vitro Target-Oriented Screening and Docking: Inhibition of Matrix Metalloproteinases Activities by Flavonoids. Planta Med. 2017 Jul;83(11):901-911.

GPLGIAGQ TFA

发表于由

GPLGIAGQ TFA; 纯度: 99.67%

GPLGIAGQ TFA 是一种 MMP2 可降解 (cleavable) 的多肽，在脂质体和胶束纳米载体中都被用作刺激敏感的连接物，用于 MMP2 触发的肿瘤靶向研究。GPLGIAGQ可用于合成光动力疗法 (PDT) 中独特的MMP2靶向光敏剂。

GPLGIAGQ TFAamp;;

GPLGIAGQ TFA Chemical Structure

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ￥1360 In-stock
5 mg ￥1000 In-stock
10 mg ￥1500 In-stock
50 mg ￥3500 In-stock
100 mg ; 询价 ;
200 mg ; 询价 ;

* Please select Quantity before adding items.

GPLGIAGQ TFA 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Apoptosis Compound Library
  • Metabolism/Protease Compound Library
  • Anti-Cancer Compound Library
  • Anti-Pancreatic Cancer Compound Library
  • Angiogenesis Related Compound Library
  • Peptide Library

生物活性

GPLGIAGQ TFA, a MMP2-cleavable polypeptide, is used as a stimulus-sensitive linker in both liposomal and micellar nanocarriers for MMP2-triggered tumor targeting. GPLGIAGQ TFA can be used to synthesis unique MMP2-targeted photosensitizer in photodynamic therapy (PDT)[1][2][3].

IC50 Target

MMP2[1].
体外研究
(In Vitro)

GPLGIAGQ is used to trigger PEG deshielding of liposomal carriers, resulting in enhanced cellular internalization[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

825.83

Formula

C33H54F3N9O12
Sequence

Gly-Pro-Leu-Gly-Ile-Ala-Gly-Gln
Sequence Shortening

GPLGIAGQ
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)
溶解性数据
In Vitro:;

DMSO : 50 mg/mL (60.55 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.2109 mL 6.0545 mL 12.1090 mL
5 mM 0.2422 mL 1.2109 mL 2.4218 mL
10 mM 0.1211 mL 0.6055 mL 1.2109 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (3.03 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.03 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (3.03 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.03 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
  • 3.

    请依序添加每种溶剂：;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.5 mg/mL (3.03 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.03 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献

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    [2]. Zhu L, et al. Matrix metalloproteinase 2-sensitive multifunctional polymeric micelles for tumor-specific co-delivery of siRNA and hydrophobic drugs. Biomaterials. 2014 Apr;35(13):4213-22.

    [3]. Liu FH, et al. Enzyme-sensitive cytotoxic peptide-dendrimer conjugates enhance cell apoptosis and deep tumor penetration. Biomater Sci. 2018 Feb 27;6(3):604-613.