RETF-4NA

RETF-4NA;

RETF-4NA 是糜蛋白酶的一个敏感的、特异性底物。

RETF-4NAamp;;

RETF-4NA Chemical Structure

CAS No. : 1160928-63-1

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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RETF-4NA 的其他形式现货产品:

RETF-4NA TFA

生物活性

RETF-4NA, a chymase-specific substrate, is a sensitive and selective substrate for chymase when free or bound to α2M[1][2].

分子量

713.74

Formula

C32H43N9O10

CAS 号

1160928-63-1

Sequence

Ac-Arg-Glu-Thr-Phe-pNA

Sequence Shortening

Ac-RETF-pNA

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献
  • [1]. Wilfred W Raymond, et al. Alpha 2-macroglobulin Capture Allows Detection of Mast Cell Chymase in Serum and Creates a Reservoir of Angiotensin II-generating Activity. J Immunol. 2009 May 1;182(9):5770-7.

    [2]. Katariina Maaninka, et al. Human Mast Cells Arise From a Common Circulating Progenitor. J Allergy Clin Immunol. 2013 Aug;132(2):463-9.e3.

荧光染料SBFI-AM

荧光染料Dye Reagents SBFI-AM;

SBFI-AM 是一种 Na+ 选择性的荧光指示剂。SBFI-AM 显示出对 Na+ 的选择性高于对 K+ 的选择性。

SBFI-AM

SBFI-AM Chemical Structure

CAS No. : 129423-53-6

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

SBFI-AM is a Na+ selective fluorescent indicator. SBFI-AM shows selectivity for Na+ over K+[1].

分子量

1127.06

Formula

C56H58N2O23

CAS 号

129423-53-6

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Minta A, et, al. Fluorescent indicators for cytosolic sodium. J Biol Chem. 1989 Nov 15;264(32):19449-57.

Strophanthidin(Synonyms: 葡萄球菌素)

天然产物 糖类和糖苷 Saccharides and Glycosides

Strophanthidin;(Synonyms: 葡萄球菌素) 纯度: 92.66%

Strophanthidin 是一种天然的心脏糖苷。Strophanthidin 0.1 和 1 nmol/L 增加 Na+/K+-ATPase 活性,1~100 μ mol/L 抑制 Na+/K+-ATPase 活性,而 Strophanthidin 10 和 100 nmol/L 对 Na+/K+-ATPase活性无影响。Strophanthidin 增加舒张期和收缩期胞内 Ca2+ 浓度。

Strophanthidin(Synonyms: 葡萄球菌素)

Strophanthidin Chemical Structure

CAS No. : 66-28-4

规格 价格 是否有货 数量
5 mg ¥1000 In-stock
10 mg ¥1500 In-stock
25 mg ¥2900 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Strophanthidin 相关产品

bull;相关化合物库:

  • Natural Product Library Plus
  • Bioactive Compound Library Plus
  • Membrane Transporter/Ion Channel Compound Library
  • Natural Product Library
  • Anti-Cardiovascular Disease Compound Library
  • Traditional Chinese Medicine Monomer Library

生物活性

Strophanthidin is a naturally available cardiac glycoside[1]. Strophanthidin 0.1 and 1 nmol/L increases and 1~100 µmol/L inhibits the Na+/K+-ATPase activities, but Strophanthidin 10 and 100 nmol/L does not affect Na+/K+-ATPase activities in cardiac sarcolemmal[2]. Strophanthidin increases both diastolic and systolic intracellular Ca2+ concentration[3].

IC50 Target

Na+/K+-ATPase[2]

体外研究
(In Vitro)

Strophanthidin (0~10 μM; 24 hours; MCF-7, A549, and HepG2 cells) is effective at suppressing the growth of cancer cells and has no toxicity in normal cells[1].
Strophanthidin (0.5 to 500 µM; PBMCs) does not show significant cytotoxicity in PBMCs. Strophanthidin (2 µM; MCF-7 cells) can arrest cell cycle at the G2/M phase[1].
Strophanthidin (MCF-7, A549, and HepG2 cells) is effective at suppressing the growth of cancer cells and has no toxicity in normal cells. Strophanthidin (MCF-7, A549, and HepG2 cells) inhibits the expression of checkpoint and cyclin-dependent kinases in three cancer cells compared to untreated controls. Strophanthidin can modulate the protein localization from the nucleus to the membrane as well as to the cytoplasm. Strophanthidin is a monosaccharide cardiac glycoside with one aglycone portion and without any sugar unit. Strophanthidin induces apoptosis by the attenuation of multiple biochemical signaling pathways and by arresting cell cycle at the G2/M phase through p53-dependent and p53-independent mechanisms[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: MCF-7, A549, and HepG2 cells
Concentration: 0~10 μM
Incubation Time: 24 hours
Result: Inhibited the proliferation in three different cancer cells.

分子量

404.50

Formula

C23H32O6

CAS 号

66-28-4

中文名称

葡萄球菌素

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20deg;C 3 years
4deg;C 2 years
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

Ethanol : 25 mg/mL (61.80 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.4722 mL 12.3609 mL 24.7219 mL
5 mM 0.4944 mL 2.4722 mL 4.9444 mL
10 mM 0.2472 mL 1.2361 mL 2.4722 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% EtOH ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (6.18 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (6.18 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 EtOH 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% EtOH ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (6.18 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (6.18 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 EtOH 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂:;10% EtOH ;; 90% corn oil

    Solubility: ≥ 2.5 mg/mL (6.18 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (6.18 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 EtOH 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Reddy D, et al. Strophanthidin Attenuates MAPK, PI3K/AKT/mTOR, and Wnt/β-Catenin Signaling Pathways in Human Cancers. Front Oncol. 2020;9:1469. Published 2020 Jan 17.

    [2]. Su SW, et al. Relationship between cardiotonic effects and inhibition on cardiac sarcolemmal Na+,K+-ATPase of strophan-thidin at low concentrations. Acta Pharmacol Sin. 2003;24(11):1103-1107.

    [3]. Bennett DL, et al. Strophanthidin-induced gain of Ca2+ occurs during diastole and not systole in guinea-pig ventricular myocytes. Pflugers Arch. 1999;437(5):731-736.

RETF-4NA TFA

RETF-4NA TFA; 纯度: 99.65%

RETF-4NA TFA 是糜蛋白酶的一个敏感的、特异性底物。

RETF-4NA TFAamp;;

RETF-4NA TFA Chemical Structure

规格 价格 是否有货 数量
5 mg ¥850 In-stock
10 mg ¥1400 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

RETF-4NA TFA 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

RETF-4NA TFA, a chymase-specific substrate, is a sensitive and selective substrate for chymase when free or bound to α2M[1][2].

分子量

827.76

Formula

C34H44F3N9O12

Sequence

Ac-Arg-Glu-Thr-Phe-pNA

Sequence Shortening

Ac-RETF-pNA

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture and light

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)

溶解性数据
In Vitro:;

DMSO : 110 mg/mL (132.89 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.2081 mL 6.0404 mL 12.0808 mL
5 mM 0.2416 mL 1.2081 mL 2.4162 mL
10 mM 0.1208 mL 0.6040 mL 1.2081 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.75 mg/mL (3.32 mM); Clear solution

    此方案可获得 ≥ 2.75 mg/mL (3.32 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 27.5 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.75 mg/mL (3.32 mM); Clear solution

    此方案可获得 ≥ 2.75 mg/mL (3.32 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 27.5 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂:;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.75 mg/mL (3.32 mM); Clear solution

    此方案可获得 ≥ 2.75 mg/mL (3.32 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 27.5 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Wilfred W Raymond, et al. Alpha 2-macroglobulin Capture Allows Detection of Mast Cell Chymase in Serum and Creates a Reservoir of Angiotensin II-generating Activity. J Immunol. 2009 May 1;182(9):5770-7.

    [2]. Katariina Maaninka, et al. Human Mast Cells Arise From a Common Circulating Progenitor. J Allergy Clin Immunol. 2013 Aug;132(2):463-9.e3.