生化分析试剂 Biochemical Assay Reagents
4-Nitrophenyl phosphate disodium hexahydrate;(Synonyms: p-Nitrophenyl phosphate disodium hexahydrate)
4-Nitrophenyl phosphate (p-nitrophenyl phosphate) disodium hexahydrate 广泛用作蛋白质酪氨酸磷酸酶活性测定中的小分子磷酸酪氨酸样底物。4-Nitrophenyl phosphate disodium hexahydrate 是一种无色基质,水解后转化为黄色的4-硝基苯酚离子,可在 405 nm 处通过吸光度监测。
4-Nitrophenyl phosphate disodium hexahydrate Chemical Structure
CAS No. : 333338-18-4
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生物活性 |
4-Nitrophenyl phosphate (p-nitrophenyl phosphate) disodium hexahydrate is widely used as a small molecule phosphotyrosine-like substrate in activity assays for protein tyrosine phosphatases. 4-Nitrophenyl phosphate disodium hexahydrate is a colorless substrate that upon hydrolysis is converted to a yellow 4-nitrophenolate ion that can be monitored by absorbance at 405 nm[1]. |
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体外研究 (In Vitro) |
4-Nitrophenyl phosphate (PNPP) disodium hexahydrate is a commonly used substrate for alkaline phosphatases (ALPs). 4-Nitrophenyl phosphate disodium hexahydrate is hydrolyzed by ALP to PNP (p-nitrophenol), which quenches the fluorescence of novel gold nanoclusters (AuNCs) by the inner filter effect (IFE)[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
分子量 |
371.14 |
Formula |
C6H16NNa2O12P |
CAS 号 |
333338-18-4 |
运输条件 |
Room temperature in continental US; may vary elsewhere. |
储存方式 |
-20deg;C, sealed storage, away from moisture *In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture) |
参考文献 |
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