Parathyroid Hormone (1-34), human, biotinylated

Parathyroid Hormone (1-34), human, biotinylated;

Parathyroid Hormone (1-34), human, biotinylated 是甲状旁腺激素受体的探针,可用于分析活细胞中甲状旁腺激素和甲状旁腺激素受体之间的相互作用,并通过亲和柱纯化激素-受体复合物。

Parathyroid Hormone (1-34), human, biotinylatedamp;;

Parathyroid Hormone (1-34), human, biotinylated Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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生物活性

Parathyroid Hormone (1-34), human, biotinylated is a probe for the parathyroid hormone receptor, can be used for analyzing the interaction between parathyroid hormone and parathyroid hormone receptors in living cells and for purifying hormone-receptor complexes with affinity columns[1][2].

分子量

4344.10

Formula

C191H305N57O53S3

Sequence

Biotin-Ser-Val-Ser-Glu-Ile-Gln-Leu-Met-His-Asn-Leu-Gly-Lys-His-Leu-Asn-Ser-Met-Glu-Arg-Val-Glu-Trp-Leu-Arg-Lys-Lys-Leu-Gln-Asp-Val-His-Asn-Phe

Sequence Shortening

Biotin-SVSEIQLMHNLGKHLNSMERVEWLRKKLQDVHNF

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献
  • [1]. Newman W, et, al. Biotinylated parathyroid hormone as a probe for the parathyroid hormone receptor. Structure-function analysis and detection of specific binding to cultured bone cells by flow cytometry. J Biol Chem. 1989 Oct 5; 264(28): 16359-65.

    [2]. Banerjee S, et, al. Synthesis and characterization of novel biotinylated carboxyl-terminal parathyroid hormone peptides that specifically crosslink to the CPTH-receptor. Peptides. 2006 Dec; 27(12): 3352-62.

Parathyroid Hormone (1-34), bovine

Parathyroid Hormone (1-34), bovine;

Parathyroid Hormone (1-34), bovine 是一种有效的甲状旁腺激素 (PTH) 受体激动剂。Parathyroid Hormone (1-34), bovine 增加钙和无机磷酸盐的水平。Parathyroid Hormone (1-34), bovine 可用于研究骨质疏松症。

Parathyroid Hormone (1-34), bovineamp;;

Parathyroid Hormone (1-34), bovine Chemical Structure

CAS No. : 12583-68-5

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

Parathyroid Hormone (1-34), bovine is a potent parathyroid hormone (PTH) receptor agonist. Parathyroid Hormone (1-34), bovine increases calcium and inorganic phosphate levels in vivo. Parathyroid Hormone (1-34), bovine can be used for th reseach of osteoporosis[1].

体外研究
(In Vitro)

Parathyroid Hormone (1-34), bovine (0.1-100 ng/mL; 2-20 days) are added to the medium, it inhibits osteoblast proliferation in a dose-dependent manner. In another group, bPTH are added to the culture medium from day 1 to day 10, but not from days 11 to 20, a rebound of proliferation is observed in the PTH Day 1–10 group after bPTH withdrawal[1].
Parathyroid Hormone (1-34), bovine (0.1-100 ng/mL; 2-20 days) induces diverse effects on the calcium and phosphorus content of culture medium. The calcium and phosphorus content of culture medium in the PTH-C 100 ng/mL group are higher than in teh control group[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[1]

Cell Line: MC3T3-E1 cells
Concentration: 0.1-100 ng/mL
Incubation Time: 2-20 days
Result: Resulted a decrease of osteoblast proliferationin concentration-dependent manner.
Resulted in a rebound of proliferation when PTH withdrawal.

体内研究
(In Vivo)

Parathyroid Hormone (1-34)(subcutaneous injection; 80 μg/kg; 5 days) increases serum osteocalcin concentrations without changing serum inorganic phosphate or calcium concentrations in either group of old animals. Serum 1,25-dihydroxyvitamin D concentrations are significantly higher in the PTH-treated senile female rats than the sex-matchedvehicle-treated controls[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

4108.77

Formula

C183H288N54O50S2

CAS 号

12583-68-5

Sequence Shortening

AVSEIQFMHNGKHLSSMERVEWLRKKLQDVHNF

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. B H Mitlak, et al. Intermittent administration of bovine PTH-(1-34) increases serum 1,25-dihydroxyvitamin D concentrations and spinal bone density in senile (23 month) rats. J Bone Miner Res. 1992 May;7(5):479-84.

    [2]. M Takigawa, et al. Studies on chondrocytes from mandibular condylar cartilage, nasal septal cartilage, and spheno-occipital synchondrosis in culture. I. Morphology, growth, glycosaminoglycan synthesis, and responsiveness to bovine parathyroid hormone (1-34). J Dent Res. 1984 Jan;63(1):19-22.

Parathyroid Hormone (1-34), bovine TFA

Parathyroid Hormone (1-34), bovine TFA;

Parathyroid Hormone (1-34), bovine TFA 是一种有效的甲状旁腺激素 (PTH) 受体激动剂。Parathyroid Hormone (1-34), bovine 增加钙和无机磷酸盐的水平。Parathyroid Hormone (1-34), bovine 可用于研究骨质疏松症。

Parathyroid Hormone (1-34), bovine TFAamp;;

Parathyroid Hormone (1-34), bovine TFA Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

Parathyroid Hormone (1-34), bovine TFA is a potent parathyroid hormone (PTH) receptor agonist. Parathyroid Hormone (1-34), bovine increases calcium and inorganic phosphate levels in vivo. Parathyroid Hormone (1-34), bovine can be used for th reseach of osteoporosis[1].

体外研究
(In Vitro)

Parathyroid Hormone (1-34), bovine (0.1-100 ng/mL; 2-20 days) are added to the medium, it inhibits osteoblast proliferation in a dose-dependent manner. In another group, bPTH are added to the culture medium from day 1 to day 10, but not from days 11 to 20, a rebound of proliferation is observed in the PTH Day 1–10 group after bPTH withdrawal[1].
Parathyroid Hormone (1-34), bovine (0.1-100 ng/mL; 2-20 days) induces diverse effects on the calcium and phosphorus content of culture medium. The calcium and phosphorus content of culture medium in the PTH-C 100 ng/mL group are higher than in the control group[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Parathyroid Hormone (1-34)(subcutaneous injection; 80 μg/kg; 5 days) increases serum osteocalcin concentrations without changing serum inorganic phosphate or calcium concentrations in either group of old animals. Serum 1,25-dihydroxyvitamin D concentrations are significantly higher in the PTH-treated senile female rats than the sex-matchedvehicle-treated controls[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

4222.79

Formula

C185H289F3N54O52S2

Sequence Shortening

AVSEIQFMHNGKHLSSMERVEWLRKKLQDVHNF

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. B H Mitlak, et al. Intermittent administration of bovine PTH-(1-34) increases serum 1,25-dihydroxyvitamin D concentrations and spinal bone density in senile (23 month) rats. J Bone Miner Res. 1992 May;7(5):479-84.

    [2]. M Takigawa, et al. Studies on chondrocytes from mandibular condylar cartilage, nasal septal cartilage, and spheno-occipital synchondrosis in culture. I. Morphology, growth, glycosaminoglycan synthesis, and responsiveness to bovine parathyroid hormone (1-34). J Dent Res. 1984 Jan;63(1):19-22.

Parathyroid hormone (1-34) (rat)

Parathyroid hormone (1-34) (rat); 纯度: 95.53%

Parathyroid hormone (1-34) (rat) 是甲状旁腺激素,可改善皮质和松质骨结构。

Parathyroid hormone (1-34) (rat)amp;;

Parathyroid hormone (1-34) (rat) Chemical Structure

CAS No. : 98614-76-7

规格 价格 是否有货 数量
1 mg ¥3200 In-stock
5 mg ¥9000 In-stock
10 mg ; 询价 ;
50 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

Parathyroid hormone (1-34) (rat) improves both cortical and cancellous bone structure[1].

体内研究
(In Vivo)

PTH (80 μg/kg per day (hPTH), s.c., 4 weeks ) induces a ~35% recovery of this loss and a ~40% reversal of the associated decreases in trabecular number (Tb.N) and connectivity. PTH also causes a shift from single to double calcein-labeled trabecular surfaces, a significant enhancement in the mineralizing perimeter and a respective 2- and 3-fold stimulation of the mineral appositional rate (MAR) and bone formation rate (BFR)[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Sixty-three 11-week-old virgin female Swiss-Webster mice[2].
Dosage: 80 μg/kg per day (hPTH).
Administration: S.C., 4 weeks (5 days a week).
Result: OVX osteoporotic mice respond to PTH by increased osteoblast activity and the consequent restoration of trabecular network.

分子量

4057.71

Formula

C180H291N55O48S2

CAS 号

98614-76-7

Sequence Shortening

AVSEIQLMHNLGKHLASVERMQWLRKKLQDVHNF

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.