荧光染料Oxazine 1 perchlorate(Synonyms: 噁嗪高氯酸盐)

荧光染料Dye Reagents Oxazine 1 perchlorate;(Synonyms: 噁嗪高氯酸盐) 纯度: ge;99.0%

Oxazine 1 perchlorate 是一种对称阳离子染料 (λex=653 nm,λem=666 nm)。

Oxazine 1 perchlorateamp;;(Synonyms: 噁嗪高氯酸盐)

Oxazine 1 perchlorate Chemical Structure

CAS No. : 24796-94-9

规格 价格 是否有货
5 mg ¥1900 询问价格 货期

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生物活性

Oxazine 1 perchlorate is a symmetric cationic dye (λex=653 nm, λem=666 nm).

体外研究
(In Vitro)

Oxazine 1 perchlorate (OX1) absorbs and emits light at about 653 and 666 nm in dilute aqueous solutions, respectively. It is soluble over a very wide range of polar solvents. The optical spectrum of Oxazine 1 perchlorate typically possesses an intense absorption band (λmax), which is neighbored by a shoulder at shorter wavelengths. Oxazine 1 perchlorate appears to exist almost in its monomeric form at concentrations below about 1×10-4 M in aqueous solutions. As it is expected, similar to polar ordinary liquid solvents, Oxazine 1 perchlorate shows only single fluorescence band in polar anisotropic media[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

423.89

Formula

C20H26ClN3O5

CAS 号

24796-94-9

中文名称

噁嗪高氯酸盐

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, sealed storage, away from moisture

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)

参考文献
  • [1]. Gilani AG, et al. Estimation of ground- and excited-state dipole moments of oxazine 1 in liquid and liquid crystalline media. Spectrochim Acta A Mol Biomol Spectrosc. 2011 Jun;79(1):148-55.

Kinase Assay
[1]

Oxazine 1 perchlorate doped liquid crystal samples are prepared from a pair of quartz sheets sandwiched exactly at known gap and sealed. The introduction of the dissolved Oxazine 1 perchlorate in nematic solvents is achieved by capillary action. Oxazine 1 perchlorate concentrations are chosen to be 1×10−5 M for all the liquid samples. Oxazine 1 perchlorate is studied in the nematic media up to a concentration of about 0.1%, w/w. The absorption spectra of Oxazine 1 perchlorate are recorded on a double beam spectrophotometer over a wavelength range 300 to 800 nm combined with a cell temperature controller[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Gilani AG, et al. Estimation of ground- and excited-state dipole moments of oxazine 1 in liquid and liquid crystalline media. Spectrochim Acta A Mol Biomol Spectrosc. 2011 Jun;79(1):148-55.

荧光染料Oxazine 1 perchlorate(Synonyms: 噁嗪高氯酸盐)

荧光染料Dye Reagents Oxazine 1 perchlorate;(Synonyms: 噁嗪高氯酸盐) 纯度: ge;99.0%

Oxazine 1 perchlorate 是一种对称阳离子染料 (λex=653 nm,λem=666 nm)。

Oxazine 1 perchlorate(Synonyms: 噁嗪高氯酸盐)

Oxazine 1 perchlorate Chemical Structure

CAS No. : 24796-94-9

规格 价格 是否有货
5 mg ¥1900 询问价格 货期

* Please select Quantity before adding items.

生物活性

Oxazine 1 perchlorate is a symmetric cationic dye (λex=653 nm, λem=666 nm).

体外研究
(In Vitro)

Oxazine 1 perchlorate (OX1) absorbs and emits light at about 653 and 666 nm in dilute aqueous solutions, respectively. It is soluble over a very wide range of polar solvents. The optical spectrum of Oxazine 1 perchlorate typically possesses an intense absorption band (λmax), which is neighbored by a shoulder at shorter wavelengths. Oxazine 1 perchlorate appears to exist almost in its monomeric form at concentrations below about 1×10-4 M in aqueous solutions. As it is expected, similar to polar ordinary liquid solvents, Oxazine 1 perchlorate shows only single fluorescence band in polar anisotropic media[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

423.89

Formula

C20H26ClN3O5

CAS 号

24796-94-9

中文名称

噁嗪高氯酸盐

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, sealed storage, away from moisture

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)

参考文献
  • [1]. Gilani AG, et al. Estimation of ground- and excited-state dipole moments of oxazine 1 in liquid and liquid crystalline media. Spectrochim Acta A Mol Biomol Spectrosc. 2011 Jun;79(1):148-55.

Kinase Assay
[1]

Oxazine 1 perchlorate doped liquid crystal samples are prepared from a pair of quartz sheets sandwiched exactly at known gap and sealed. The introduction of the dissolved Oxazine 1 perchlorate in nematic solvents is achieved by capillary action. Oxazine 1 perchlorate concentrations are chosen to be 1×10−5 M for all the liquid samples. Oxazine 1 perchlorate is studied in the nematic media up to a concentration of about 0.1%, w/w. The absorption spectra of Oxazine 1 perchlorate are recorded on a double beam spectrophotometer over a wavelength range 300 to 800 nm combined with a cell temperature controller[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Gilani AG, et al. Estimation of ground- and excited-state dipole moments of oxazine 1 in liquid and liquid crystalline media. Spectrochim Acta A Mol Biomol Spectrosc. 2011 Jun;79(1):148-55.

TMRM Perchlorate(Synonyms: T668)

TMRM Perchlorate;(Synonyms: T668) 纯度: 98.65%

TMRM Perchlorate 是一种细胞渗透性阳离子亲脂性红色荧光染料 (λex=530 nm, λem=592 nm)。

TMRM Perchlorateamp;;(Synonyms: T668)

TMRM Perchlorate Chemical Structure

CAS No. : 115532-50-8

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ¥1543 In-stock
5 mg ¥1400 In-stock
10 mg ¥2100 In-stock
25 mg ¥4200 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

TMRM Perchlorate 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

TMRM Perchlorate is a cell-permeant cationic lipophilic red fluorescent dye (λex=530 nm, λem=592 nm).

体外研究
(In Vitro)

TMRM Perchlorate is a fluorescent probe (excitation, 530±21 nm; emission, 592±22 nm). The fluorescence signal in the presence of TMRM Perchlorate shows a slight decrease after the addition of glutamate, indicative of increased polarization of the mitochondrial inner membrane. In the presence of TMRM Perchlorate (2 μM) the coupled respiration with Complex I substrates or upon the addition of Complex II substrate is decreased by 27%[1]. Exposure of hippocampal cultures to low concentrations of TMRM Perchlorate (50 to 500 nM) for 1 to 3 hours results in selective staining of mitochondria in both neurons and the underlying glial cells. Exposure of hippocampal cultures to high concentrations of TMRM Perchlorate (1 to 25 µM) stains mitochondria selectively and quickly, reaching a plateau after 5 to 10 min. Low concentrations of TMRM Perchlorate (50 to 200 nM) do not induce apoptosis, whereas higher concentrations (0.5 and 2.5 µM) enhance apoptosis (KD = 500 nM)[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

500.93

Formula

C25H25ClN2O7

CAS 号

115532-50-8

中文名称

四甲基罗丹明甲酯高氯酸盐

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)

溶解性数据
In Vitro:;

DMSO : 41.67 mg/mL (83.19 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.9963 mL 9.9814 mL 19.9629 mL
5 mM 0.3993 mL 1.9963 mL 3.9926 mL
10 mM 0.1996 mL 0.9981 mL 1.9963 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Chowdhury SR, et al. Simultaneous evaluation of substrate-dependent oxygen consumption rates and mitochondrial membrane potential by TMRM and safranin in cortical mitochondria. Biosci Rep. 2015 Dec 8;36(1):e00286.

    [2]. Monteith A, et al. Imaging of mitochondrial and non-mitochondrial responses in cultured rat hippocampal neurons exposed to micromolar concentrations of TMRM. PLoS One. 2013;8(3):e58059.

Cell Assay
[1]

Cultures are exposed to Millipore-filtered solutions (0.22 µm) containing TMRM Perchlorate for 1 hr at 37°C (except the experiment involving different durations of exposure to TMRM Perchlorate). After treatment, solutions are removed and growth media reapplied under sterile conditions, and cultures are post-incubated for 18 hours at 37°C (except for the experiment involving analysis at different time points after exposure). Cells are then stained with 2 mg/mL bisbenzimide for 20 min at room temperature. Coverslips are subsequently washed in saline and imaged using 2P microscopy. Apoptotic cells are identified as brightly fluorescent nuclei under UV excitation indicating DNA fragmentation. Cell survivability is calculated as the percentage of live, unstained cells (±SD) in five microscopic fields per treatment[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Chowdhury SR, et al. Simultaneous evaluation of substrate-dependent oxygen consumption rates and mitochondrial membrane potential by TMRM and safranin in cortical mitochondria. Biosci Rep. 2015 Dec 8;36(1):e00286.

    [2]. Monteith A, et al. Imaging of mitochondrial and non-mitochondrial responses in cultured rat hippocampal neurons exposed to micromolar concentrations of TMRM. PLoS One. 2013;8(3):e58059.

荧光染料Cresyl Violet perchlorate(Synonyms: Oxazine 9 perchlorate)

荧光染料Dye Reagents Cresyl Violet perchlorate;(Synonyms: Oxazine 9 perchlorate)

Cresyl violet perchlorate 是一种红色荧光染色剂,可用于染色神经元。

Cresyl Violet perchlorateamp;;(Synonyms: Oxazine 9 perchlorate)

Cresyl Violet perchlorate Chemical Structure

CAS No. : 41830-80-2

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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Cresyl Violet perchlorate 的其他形式现货产品:

Cresyl Violet acetate

生物活性

Cresyl Violet perchlorate is a red fluorescent stain, which can be used to stain neurons.

体外研究
(In Vitro)

The estimated total number of SG neurons is 27,485±3251 and 26,705±1823 in the PV and Cresyl Violet perchlorate (CV) stained sections, respectively. There is no significant difference between them (p=0.552). Therefore, Cresyl Violet perchlorate staining is simpler and more cost effective when estimates neuronal number. Although PV stains spiral ganglion neurons (SGNs) with a greater intensity and provides a functional status, its tedious protocol limits its use for quantification. Total RC volume is estimated using probe and it is found that an average RC volume of 2.162±0.35 mm3 and 1.82±0.33 mm3 in Cresyl Violet perchlorate stained and PV immunostained sections, respectively. Volume of neurons is estimated using nucleator probe and it is 3487.63±951 μm3 and 3740.1±784 μm3 in CV stained and PV immunostained sections, respectively. Similarly, volume of neuronal nucleus is also estimated using nucleator probe and it is found to be 131.68±50 μm3 and 126.51±33 μm3 in CV stained and PV immunostained sections, respectively[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

361.74

Formula

C16H12ClN3O5

CAS 号

41830-80-2

中文名称

甲酚紫高氯酸盐

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Kaur C, et al. Comparison of unbiased stereological estimation of total number of cresyl violet stained neurons and parvalbumin positive neurons in the adult human spiral ganglion. J Chem Neuroanat. 2017 Jun 23. pii: S0891-0618(17)30037-6.

Cell Assay
[1]

Cochlear sections containing SGNs are placed in 24 wells plates containing PBS (pH 7.4) and stored at 4°C. The sections are then used for Cresyl Violet perchlorate (CV) and immunohistochemical (IHC) staining. Every 7th section is stained with Cresyl Violet perchlorate (1%), dehydrated with ascending grades of alcohol, cleared with xylene, mounted with DPX and observed under microscope. Approximately 12-13 Cresyl Violet perchlorate stained sections from each specimen are used for stereology. None of these cases show any histopathological changes under the light microscope. Estimation of the total volume of the Rosenthal canal (RC), total number of SGNs (optical fractionator probe) and the volume of the soma and their nucleus (nucleator probe) is done with software[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Kaur C, et al. Comparison of unbiased stereological estimation of total number of cresyl violet stained neurons and parvalbumin positive neurons in the adult human spiral ganglion. J Chem Neuroanat. 2017 Jun 23. pii: S0891-0618(17)30037-6.

DiD perchlorate

DiD perchlorate; 纯度: 98.96%

DiD perchlorate 是一种远红色荧光亲脂性花青染料。DiD perchlorate 能快速稳定地与磷脂细胞膜结合,能用于细胞追踪。

DiD perchlorateamp;;

DiD perchlorate Chemical Structure

CAS No. : 127274-91-3

规格 价格 是否有货 数量
5 mg ¥1200 In-stock
10 mg ; 询价 ;
50 mg ; 询价 ;

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DiD perchlorate 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

DiD perchlorate is a far-red fluorescent lipophilic cyanine dye. DiD perchlorate can rapidly and stably integrate into the phospholipid cell membrane. DiD perchlorate is used to cells tracking[1][2][3].

体内研究
(In Vivo)

Two weeks after injection of stained cells, a single, bright, DiD perchlorate (DiD)-positive cells located between 15 to 40 µm from the endosteum is found. Results reveal a progressive appearance of cell clusters of decreased dye intensity, consistent with the partitioning of DiD perchlorate label on cell division[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

959.90

Formula

C61H99ClN2O4

CAS 号

127274-91-3

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, protect from light, stored under nitrogen

*该产品在溶液状态不稳定,建议您现用现配,即刻使用。

溶解性数据
In Vitro:;

DMSO : 25 mg/mL (26.04 mM; ultrasonic and warming and heat to 60°C)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.0418 mL 5.2089 mL 10.4178 mL
5 mM 0.2084 mL 1.0418 mL 2.0836 mL
10 mM 0.1042 mL 0.5209 mL 1.0418 mL

*

请根据产品在不同溶剂中的溶解度,选择合适的溶剂配制储备液;该产品在溶液状态不稳定,建议您现用现配,即刻使用

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 1.67 mg/mL (1.74 mM); Clear solution

    此方案可获得 ≥ 1.67 mg/mL (1.74 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 16.7 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 1.67 mg/mL (1.74 mM); Clear solution

    此方案可获得 ≥ 1.67 mg/mL (1.74 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 16.7 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂:;10% DMSO ;; 90% corn oil

    Solubility: ≥ 1.67 mg/mL (1.74 mM); Clear solution

    此方案可获得 ≥ 1.67 mg/mL (1.74 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 16.7 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Kenji Yumoto, et al. A novel method for monitoring tumor dormancy using fluorescent dye DiD. Cytometry A. 2014 Jun; 85(6): 548–555.

    [2]. Meng Li, et al. In Vivo Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic Membrane Dye. J Vis Exp. 2017; (128): 56273.

    [3]. Lo Celso C, et al. Live-animal tracking of individual haematopoietic stem/progenitor cells in their niche.

Animal Administration
[1]

1 to 5×105 cells per mL HSPCs are stained with 5 μM DiD perchlorate (DiD) or 7.5 μM DiI in PBS without serum for 10 min at 37°C, washed once in PBS and immediately injected into the tail vein of recipient mice. Unless stated differently, each imaged CD45.2 mouse receive 8,000 to 15,000 labelled CD45.1 HSPCs together with 3 to 5×105 CD45.2 supportive whole bone-marrow mononuclear cells to ensure survival[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Kenji Yumoto, et al. A novel method for monitoring tumor dormancy using fluorescent dye DiD. Cytometry A. 2014 Jun; 85(6): 548–555.

    [2]. Meng Li, et al. In Vivo Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic Membrane Dye. J Vis Exp. 2017; (128): 56273.

    [3]. Lo Celso C, et al. Live-animal tracking of individual haematopoietic stem/progenitor cells in their niche.