PL1512-5801-安捷伦Agilent液相色谱柱

  • 型号 PL1512-5801
  • 品牌 Agilent 安捷伦
  • 【简单介绍】

    安捷伦Agilent液相色谱柱 PL1512-5801
    PLRP-S 系列液相色谱柱,粒径8μm,孔径300Å,内径4.6mm,长度250mm。PLRP-S 系列液相色谱柱,基质为苯乙烯-二乙烯基苯共聚物,主要应用于重组多肽/蛋白质

    安捷伦Agilent液相色谱柱  PL1512-5801

     

    PLRP-S 系列液相色谱柱,粒径8μm,孔径300Å,内径4.6mm,长度250mm。PLRP-S 系列液相色谱柱,基质为苯乙烯-二乙烯基苯共聚物,主要应用于重组多肽/蛋白质

    PLRP-S 色谱柱

    * 耐用的弹性填料提供重现性结果,寿命更长

    * 热稳定和化学稳定性高

    * 遵循USP L21 标准

    * 用于生命科学、化学、临床研究、能源、环境、食品和农业、材料科学和制药行业

    * 宽孔径范围(100A-4000A),适合于分离小分子到大分子复合物和多核苷酸

    PLRP-S 系列色谱柱包括各种孔径和填料尺寸,它们都具有相同的化学特性和基本吸附特性。这些

    填料本质上是疏水的,因此不需要键合相、烷基配体来进行反相分离。因此,能得到无硅醇基、无

    重金属离子的高重现性填料。该色谱柱拥有多种产品系列,适用于纳流/微量分离,包括自下而上

    和自上而下的蛋白质组学、分析型分离以及制备级纯化。此外,Process 色谱柱可以用大量散装填

    料进行装填。

    安捷伦Agilent液相色谱柱  PL1512-5801

    PL1512-5801-安捷伦Agilent液相色谱柱

PL-017

PL-017;

PL-017 是一种有效的,选择性 μ 阿片受体 (μ opioid receptor) 激动剂,对 125I-FK 33,824 与 μ 位点结合的 IC50 为 5.5 nM。PL-017 在大鼠中产生持久的可逆性缓解疼痛作用。

PL-017amp;;

PL-017 Chemical Structure

CAS No. : 83397-56-2

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

PL-017 is a potent and selective μ opioid receptor agonist with an IC50 of 5.5 nM for 125I-FK 33,824 binding to μ site. PL-017 produces long-lasting, reversible analgesia in rats[1].

IC50 Target

IC50: 5.5 nM (125I-FK 33,824 binding to μ site)[1]

体外研究
(In Vitro)

PL-017 has an IC50 of 10000 nM for 125I-DADLE binding to δ site[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

PL-017 (0.22, 0.45, 0.9 nmol/rat; i.c.v.) has the time of maximum effect of approximately 15 to 30 mm after injection at lower doses and produces analgesia lasting as long as 4 hours with larger doses in male Sprague-Dawley rats weighing 250 to 375 g[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

535.63

Formula

C29H37N5O5

CAS 号

83397-56-2

Sequence Shortening

YP-{N(Me)Phe}-P-NH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献
  • [1]. K J Chang, et al. Potent morphiceptin analogs: structure activity relationships and morphine-like activities. J Pharmacol Exp Ther. 1983 Nov;227(2):403-8.

PL-017 TFA

PL-017 TFA;

PL-017 TFA 是一种有效的,选择性 μ 阿片受体 (μ opioid receptor) 激动剂,对125I-FK 33,824 与 μ 位点结合的 IC50 为 5.5 nM。PL-017 TFA 在大鼠中产生持久的可逆性缓解疼痛作用。

PL-017 TFAamp;;

PL-017 TFA Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

PL-017 TFA is a potent and selective μ opioid receptor agonist with an IC50 of 5.5 nM for 125I-FK 33,824 binding to μ site. PL-017 TFA produces long-lasting, reversible analgesia in rats[1].

IC50 Target

IC50: 5.5 nM (125I-FK 33,824 binding to μ site)[1]

体外研究
(In Vitro)

PL-017 TFA has an IC50 of 10000 nM for 125I-DADLE binding to δ site[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

PL-017 TFA (0.22, 0.45, 0.9 nmol/rat; i.c.v.) has the time of maximum effect of approximately 15 to 30 mm after injection at lower doses and produces analgesia lasting as long as 4 hours with larger doses in male Sprague-Dawley rats weighing 250 to 375 g[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

649.66

Formula

C31H38F3N5O7

Sequence Shortening

YP-{N(Me)Phe}-P-NH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献
  • [1]. K J Chang, et al. Potent morphiceptin analogs: structure activity relationships and morphine-like activities. J Pharmacol Exp Ther. 1983 Nov;227(2):403-8.

荧光染料PL553

荧光染料Dye Reagents PL553;

PL553 是一种特异性的,具有高度亲和性的白三烯 A4 水解酶 (Leukotriene A4 hydrolase) 荧光发生底物,最大吸收光和发射光分别为 210 nm 和 410 nm。

PL553

PL553 Chemical Structure

CAS No. : 1456872-74-4

规格 价格 是否有货
5 mg ¥3500 询问价格 货期
10 mg ¥6000 询问价格 货期
25 mg ¥12000 询问价格 货期

* Please select Quantity before adding items.

生物活性

PL553 is a specific and high-affinity fluorigenic substrate of Leukotriene A4 hydrolase, with a λmax of 210 nm and λem of 410 nm.

体外研究
(In Vitro)

PL553 is a specific and high-affinity fluorigenic substrate of Leukotriene A4 hydrolase (LTA4H), with a maximum absorption (λmax) of 210 nm and maximum emission (λem) of 410 nm. PL553 is a better LTA4H substrate than (l)-Ala-β-naphthylamide, and resistant to cleavage by other aminopeptidases, but can be cleaved by FAAH. PL553 (40 μM) is used to evaluate the potencies of known inhibitors toward LTA4H[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

508.49

Formula

C28H23F3N2O4

CAS 号

1456872-74-4

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Poras H, et al. A sensitive fluorigenic substrate for selective in vitro and in vivo assay of leukotriene A4 hydrolase activity. Anal Biochem. 2013 Oct 15;441(2):152-61.

Kinase Assay
[1]

The ability of PL553 to discriminate LTA4H from APN enzymatic activity is tested using the (l)-Ala-β-naphthylamide and PL553 peptide substrates at 40 μM with either APN from porcine kidney (0.33 mU/mL) or recombinant human LTA4H (0.6 μg/mL). The enzymatic reactions proceeds for 1 h at 37°C in a final volume of 100 μL of 50 mM Tris-HCl (pH 7.4) or 50 mM Tris-HCl (pH 7.4) and 100 mM NaCl for APN or LTA4H, respectively[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Poras H, et al. A sensitive fluorigenic substrate for selective in vitro and in vivo assay of leukotriene A4 hydrolase activity. Anal Biochem. 2013 Oct 15;441(2):152-61.