荧光染料Dye Reagents PL553;
PL553 是一种特异性的，具有高度亲和性的白三烯 A4 水解酶 (Leukotriene A4 hydrolase) 荧光发生底物，最大吸收光和发射光分别为 210 nm 和 410 nm。
PL553 Chemical Structure
CAS No. : 1456872-74-4
| 规格 | 价格 | 是否有货 | |
|---|---|---|---|
| 5 mg | ￥3500 | 询问价格 货期 | |
| 10 mg | ￥6000 | 询问价格 货期 | |
| 25 mg | ￥12000 | 询问价格 货期 | |
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| 生物活性 |
PL553 is a specific and high-affinity fluorigenic substrate of Leukotriene A4 hydrolase, with a λmax of 210 nm and λem of 410 nm. |
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| 体外研究 (In Vitro) |
PL553 is a specific and high-affinity fluorigenic substrate of Leukotriene A4 hydrolase (LTA4H), with a maximum absorption (λmax) of 210 nm and maximum emission (λem) of 410 nm. PL553 is a better LTA4H substrate than (l)-Ala-β-naphthylamide, and resistant to cleavage by other aminopeptidases, but can be cleaved by FAAH. PL553 (40 μM) is used to evaluate the potencies of known inhibitors toward LTA4H[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
| 分子量 |
508.49 |
| Formula |
C28H23F3N2O4 |
| CAS 号 |
1456872-74-4 |
| 运输条件 |
Room temperature in continental US; may vary elsewhere. |
| 储存方式 |
Please store the product under the recommended conditions in the Certificate of Analysis. |
| 参考文献 |
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| Kinase Assay [1] |
The ability of PL553 to discriminate LTA4H from APN enzymatic activity is tested using the (l)-Ala-β-naphthylamide and PL553 peptide substrates at 40 μM with either APN from porcine kidney (0.33 mU/mL) or recombinant human LTA4H (0.6 μg/mL). The enzymatic reactions proceeds for 1 h at 37°C in a final volume of 100 μL of 50 mM Tris-HCl (pH 7.4) or 50 mM Tris-HCl (pH 7.4) and 100 mM NaCl for APN or LTA4H, respectively[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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| 参考文献 |
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