标签归档：protease

HIV Protease Substrate 1

发表于由

HIV Protease Substrate 1;

HIV Protease Substrate 1 是一种外源性 HIV 蛋白酶底物，可用于研究 HIV 蛋白酶的酶活性。

HIV Protease Substrate 1amp;;

HIV Protease Substrate 1 Chemical Structure

CAS No. : 223769-59-3

规格 是否有货
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250 mg ; 询价 ;
500 mg ; 询价 ;

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生物活性

HIV Protease Substrate 1, a fiuorogenic HIV protease substrate, can be used to study enzymatic activity of HIV protease[1].

体外研究
(In Vitro)

HIV Protease Substrate 1 is a synthetic peptide sequence that contains the cleavage site (Tyr-Pro) for HIV protease as well as two covalently modified amino acids for the detection of cleavage[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

2017.27

Formula

C92H133N27O23S
CAS 号

223769-59-3
Sequence

Arg-{Glu(EDANS)}-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-Lys{(DABCYL)}-Arg
Sequence Shortening

R{Glu(EDANS)}SQNYPIVQ{Lys(DABCYL)}R
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Kapewangolo P, et al. Anti-HIV Activity of Ocimum labiatum Extract and Isolated Pheophytin-a. Molecules. 2017;22(11):1763. Published 2017 Nov 6.

Protease-Activated Receptor-1, PAR-1 Agonist

发表于由

Protease-Activated Receptor-1, PAR-1 Agonist;

Protease-Activated Receptor-1, PAR-1 Agonist 是一种选择性蛋白酶激活受体1 (PAR-1) 激动肽。Protease-Activated Receptor-1, PAR-1 Agonist 对应于PAR1配体，可通过该受体选择性地模拟凝血酶的作用。

Protease-Activated Receptor-1, PAR-1 Agonistamp;;

Protease-Activated Receptor-1, PAR-1 Agonist Chemical Structure

CAS No. : 141136-85-8

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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Protease-Activated Receptor-1, PAR-1 Agonist 的其他形式现货产品:

Protease-Activated Receptor-1, PAR-1 Agonist TFA

生物活性

Protease-Activated Receptor-1, PAR-1 Agonist is a selective proteinase-activated receptor1 (PAR-1) agonist peptide. Protease-Activated Receptor-1, PAR-1 Agonist corresponds to PAR1 tethered ligand and which can selectively mimic theactions of thrombin via this receptor[1][2].

IC50 Target

PAR-1[1]
体外研究
(In Vitro)

Protease-Activated Receptor-1, PAR-1 Agonist induces activation of protein kinase C isoenzymes alpha and epsilon in human HT-29 colon carcinoma cells expressing PAR1 endogeneously. On the cellular level, Protease-Activated Receptor-1, PAR-1 Agonist and thrombin prompted HT-29 cell migration and matrix adhesion by a PKCepsilon-dependent mechanism as concluded because of the inhibition of PAR1-mediated effects by the PKC inhibitor bisindolylmaleimide I and the PKCepsilon translocation inhibitory peptide EAVSLKPT but not by the PKC inhibitor Gö 6976[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

762.90

Formula

C35H58N10O9
CAS 号

141136-85-8
Sequence

Thr-Phe-Leu-Leu-Arg-Asn
Sequence Shortening

TFLLRN
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Stefanie Gödecke, et al. Thrombin-induced ATP release from human umbilical vein endothelial cells. Am J Physiol Cell Physiol. 2012 Mar 15;302(6):C915-23.

    [2]. Heider I, et al. PAR1-type thrombin receptor stimulates migration and matrix adhesion of human colon carcinoma cells by a PKCepsilon-dependent mechanism. Oncol Res. 2004;14(10):475-482.

Hepatitis Virus C NS3 Protease Inhibitor 2

发表于由

Hepatitis Virus C NS3 Protease Inhibitor 2;

Hepatitis Virus C NS3 Protease Inhibitor 2 是基于产物的丙型肝炎病毒蛋白酶 ( HCV NS3 protease) 肽抑制剂，其 Ki 值为 41 nM。

Hepatitis Virus C NS3 Protease Inhibitor 2amp;;

Hepatitis Virus C NS3 Protease Inhibitor 2 Chemical Structure

CAS No. : 208939-95-1

规格 是否有货
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250 mg ; 询价 ;
500 mg ; 询价 ;

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生物活性

Hepatitis Virus C NS3 Protease Inhibitor 2 is a product-based peptide inhibitor of hepatitis C virus (HCV) NS3 protease, with a Ki of 41 nM[1].

IC50 Target

HCV NS3 Protease[1]
分子量

913.10

Formula

C43H56N6O14S
CAS 号

208939-95-1
Sequence

Ac-Asp-Glu-{Dif}-Glu-{Cha}-Cys
Sequence Shortening

Ac-DE-{Dif}-E-{Cha}-C
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. A Johansson, et al. Inhibition of hepatitis C virus NS3 protease activity by product-based peptides is dependent on helicase domain. Bioorg Med Chem Lett. 2001 Jan 22;11(2):203-6.

HIV Protease Substrate 1 TFA

发表于由

HIV Protease Substrate 1 TFA;

HIV Protease Substrate 1 TFA 是一种外源性 HIV 蛋白酶底物，可用于研究 HIV 蛋白酶的酶活性。

HIV Protease Substrate 1 TFAamp;;

HIV Protease Substrate 1 TFA Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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生物活性

HIV Protease Substrate 1 TFA, a fiuorogenic HIV protease substrate, can be used to study enzymatic activity of HIV protease[1].

分子量

2131.29

Formula

C94H134F3N27O25S
Sequence

Arg-{Glu(EDANS)}-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-Lys{(DABCYL)}-Arg
Sequence Shortening

R{Glu(EDANS)}SQNYPIVQ{Lys(DABCYL)}R
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Kapewangolo P, et al. Anti-HIV Activity of Ocimum labiatum Extract and Isolated Pheophytin-a. Molecules. 2017;22(11):1763. Published 2017 Nov 6.

Protease-Activated Receptor-3 (PAR-3) (1-6), human

发表于由

Protease-Activated Receptor-3 (PAR-3) (1-6), human;

Protease-Activated Receptor-3 (PAR-3) (1-6), human 是一个蛋白酶活化受体 3 (PAR-3) 的激动剂肽。

Protease-Activated Receptor-3 (PAR-3) (1-6), humanamp;;

Protease-Activated Receptor-3 (PAR-3) (1-6), human Chemical Structure

CAS No. : 1872435-09-0

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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Protease-Activated Receptor-3 (PAR-3) (1-6), human 的其他形式现货产品:

Protease-Activated Receptor-3 (PAR-3) (1-6), human TFA

生物活性

Protease-Activated Receptor-3 (PAR-3) (1-6), human is a proteinase-activated receptor (PAR-3) agonist peptide[1].

IC50 Target

PAR-3[1]
分子量

646.74

Formula

C29H46N10O7
CAS 号

1872435-09-0
Sequence Shortening

TFRGAP-NH2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Liora Segal, et al. Proteinase-activated receptors differentially modulate in vitro invasion of human pancreatic adenocarcinoma PANC-1 cells in correlation with changes in the expression of CDC42 protein. Pancreas. 2014 Jan; 43(1): 10.1097/MPA.0b013e31829f0b81.

SLIGRL-NH2(Synonyms: Protease-Activated Receptor-2 Activating Peptide)

发表于由

SLIGRL-NH2;(Synonyms: Protease-Activated Receptor-2 Activating Peptide) 纯度: 99.66%

SLIGRL-NH2 (Protease-Activated Receptor-2 Activating Peptide) 是一种蛋白酶激活受体-2 (PAR-2) 激动剂。

SLIGRL-NH2amp;;(Synonyms: Protease-Activated Receptor-2 Activating Peptide)

SLIGRL-NH2 Chemical Structure

CAS No. : 171436-38-7

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ￥3179 In-stock
1 mg ￥800 In-stock
5 mg ￥2200 In-stock
10 mg ￥3900 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

SLIGRL-NH2 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • GPCR/G Protein Compound Library
  • Immunology/Inflammation Compound Library
  • Peptide Library

生物活性

SLIGRL-NH2 (Protease-Activated Receptor-2 Activating Peptide) is an agonist of Protease-Activated Receptor-2 (PAR-2)[1].

IC50 Target

PAR-2[1]
体外研究
(In Vitro)

SLIGRL-NH2 is an agonist of PAR-2 and MrgprC11[1]. SLIGRL-NH2 causes an L-NAME-inhibited relaxation. Based on SLIGRL-NH2 causing a concentration-dependent relaxation with an EC50 of 10 µM in endothelium-free preparations in the presence of perivascular adipose tissue (PVAT) , 20 µM is used as a suitable ‘test’ concentration of peptide in subsequent experiments designed to evaluate the effects of potential inhibitors of ADRF release/action. In the endothelium-free aorta preparations, SLIGRL-NH2 causes a concentration-dependent relaxation in preparations only in the presence of PVAT [+PVAT, -ENDO (endothelium)][2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

656.82

Formula

C29H56N10O7
CAS 号

171436-38-7
Sequence

Ser-Leu-Ile-Gly-Arg-Leu-NH2
Sequence Shortening

SLIGRL-NH2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

H2O : 110 mg/mL (167.47 mM; Need ultrasonic)

DMSO : 100 mg/mL (152.25 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.5225 mL 7.6124 mL 15.2249 mL
5 mM 0.3045 mL 1.5225 mL 3.0450 mL
10 mM 0.1522 mL 0.7612 mL 1.5225 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (3.81 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.81 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (3.81 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.81 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
  • 3.

    请依序添加每种溶剂：;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.5 mg/mL (3.81 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.81 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Akiyama T, et al. Behavioral model of itch, alloknesis, pain and allodynia in the lower hindlimb and correlativeresponses of lumbar dorsal horn neurons in the mouse. Neuroscience. 2014 Apr 25;266:38-46.

    [2]. Li Y, et al. Perivascular adipose tissue-derived relaxing factors: release by peptide agonists via proteinase-activated receptor-2 (PAR2) and non-PAR2 mechanisms. Br J Pharmacol. 2011 Dec;164(8):1990-2002.

Proteinase K(Synonyms: Protease K)

发表于由

Proteinase K;(Synonyms: Protease K) 纯度: ge;95.0%

Proteinase K (Protease K) 是可用于蛋白消化的一种非特异性丝氨酸蛋白酶。Proteinase K 在有 SDS 或尿素存在的情况下，在很大 pH 值 (4-12)、盐浓度和温度单位内均有活性。

Proteinase Kamp;;(Synonyms: Protease K)

Proteinase K Chemical Structure

CAS No. : 39450-01-6

规格 价格 是否有货 数量
100 mg ￥1200 In-stock
500 mg ￥3500 In-stock
1 g ; 询价 ;
5 g ; 询价 ;

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Proteinase K 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Proteinase K (Protease K) is a nonspecific serine protease that is useful for general digestion of proteins. Proteinase K is active in the presence of SDS or urea and over a wide range of pH (4-12), salt concentrations, and temperatures[1].

CAS 号

39450-01-6
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

H2O : 25 mg/mL (Need ultrasonic)
参考文献

  • [1]. W Ebeling, et al. Proteinase K From Tritirachium Album Limber. Eur J Biochem . 1974 Aug 15;47(1):91-7.

Protease-Activated Receptor-1, PAR-1 Agonist TFA

发表于由

Protease-Activated Receptor-1, PAR-1 Agonist TFA; 纯度: 99.08%

Protease-Activated Receptor-1, PAR-1 Agonist TFA 是一种选择性蛋白酶激活受体1 (PAR-1) 激动肽。Protease-Activated Receptor-1, PAR-1 Agonist TFA 对应于 PAR1 配体，可通过该受体选择性地模拟凝血酶的作用。

Protease-Activated Receptor-1, PAR-1 Agonist TFAamp;;

Protease-Activated Receptor-1, PAR-1 Agonist TFA Chemical Structure

规格 价格 是否有货 数量
10 mg ￥4250 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Protease-Activated Receptor-1, PAR-1 Agonist TFA 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • GPCR/G Protein Compound Library
  • Immunology/Inflammation Compound Library
  • Anti-Cancer Compound Library
  • Peptide Library

生物活性

Protease-Activated Receptor-1, PAR-1 Agonist TFA is a selective proteinase-activated receptor1 (PAR-1) agonist peptide. Protease-Activated Receptor-1, PAR-1 Agonist TFA corresponds to PAR1 tethered ligand and which can selectively mimic theactions of thrombin via this receptor[1][2].

体外研究
(In Vitro)

Protease-Activated Receptor-1, PAR-1 Agonist induces activation of protein kinase C isoenzymes alpha and epsilon in human HT-29 colon carcinoma cells expressing PAR1 endogeneously. On the cellular level, Protease-Activated Receptor-1, PAR-1 Agonist and thrombin prompted HT-29 cell migration and matrix adhesion by a PKCepsilon-dependent mechanism as concluded because of the inhibition of PAR1-mediated effects by the PKC inhibitor bisindolylmaleimide I and the PKCepsilon translocation inhibitory peptide EAVSLKPT but not by the PKC inhibitor Gö 6976[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

876.92

Formula

C37H59F3N10O11
Sequence

Thr-Phe-Leu-Leu-Arg-Asn
Sequence Shortening

TFLLRN
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)
溶解性数据
In Vitro:;

H2O : 6.67 mg/mL (7.61 mM; ultrasonic and warming and heat to 60°C)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.1404 mL 5.7018 mL 11.4035 mL
5 mM 0.2281 mL 1.1404 mL 2.2807 mL
10 mM — — —

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Stefanie Gödecke, et al. Thrombin-induced ATP release from human umbilical vein endothelial cells. Am J Physiol Cell Physiol. 2012 Mar 15;302(6):C915-23.

    [2]. Heider I, et al. PAR1-type thrombin receptor stimulates migration and matrix adhesion of human colon carcinoma cells by a PKCepsilon-dependent mechanism. Oncol Res. 2004;14(10):475-482.

Protease-Activated Receptor-4(Synonyms: 蛋白酶活化的受体-4)

发表于由

Protease-Activated Receptor-4;(Synonyms: 蛋白酶活化的受体-4) 纯度: 98.14%

Protease-Activated Receptor-4 是转化生长因子受体 4 的激动剂。

Protease-Activated Receptor-4amp;;(Synonyms: 蛋白酶活化的受体-4)

Protease-Activated Receptor-4 Chemical Structure

CAS No. : 245443-52-1

规格 价格 是否有货 数量
5 mg ￥880 In-stock
10 mg ￥1550 In-stock
25 mg ￥3400 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Protease-Activated Receptor-4 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Protease-Activated Receptor-4 is the agonist of proteinase-activated receptor-4 (PAR4).

体外研究
(In Vitro)

GYPGKF-NH2 significantly reduces the agonistic potency of AYPGKF-NH2 by 25-fold[1]. GYPGKF-NH2 (500 μM) does not cause contraction or relaxation of the guinea pig IAS strips[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

666.77

Formula

C33H46N8O7
CAS 号

245443-52-1
Sequence

Gly-Tyr-Pro-Gly-Lys-Phe-NH2
Sequence Shortening

GYPGKF-NH2
中文名称

蛋白酶活化的受体-4
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

H2O : 50 mg/mL (74.99 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.4998 mL 7.4988 mL 14.9977 mL
5 mM 0.3000 mL 1.4998 mL 2.9995 mL
10 mM 0.1500 mL 0.7499 mL 1.4998 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Moschonas IC, et al. Molecular requirements involving the human platelet protease-activated receptor-4 mechanism of activation by peptide analogues of its tethered-ligand. Platelets. 2017 Mar 7:1-10. doi: 10.1080/09537104.2017.1282607. [Epub ahead of print]

    [2]. Huang SC, et al. Proteinase-activated receptor-1 (PAR1) and PAR2 mediate relaxation of guinea pig internal anal sphincter. Regul Pept. 2014 Feb 10;189:46-50.

Protease-Activated Receptor-3 (PAR-3) (1-6), human TFA

发表于由

Protease-Activated Receptor-3 (PAR-3) (1-6), human TFA; 纯度: 98.85%

Protease-Activated Receptor-3 (PAR-3) (1-6), human TFA 是一个蛋白酶活化受体 3 (PAR-3) 的激动剂肽。

Protease-Activated Receptor-3 (PAR-3) (1-6), human TFAamp;;

Protease-Activated Receptor-3 (PAR-3) (1-6), human TFA Chemical Structure

规格 价格 是否有货 数量
5 mg ￥1000 In-stock
10 mg ￥1700 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

Protease-Activated Receptor-3 (PAR-3) (1-6), human TFA is a proteinase-activated receptor (PAR-3) agonist peptide[1].

IC50 Target

PAR-3[1]
分子量

760.76

Formula

C31H47F3N10O9
Sequence Shortening

TFRGAP-NH2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)
参考文献

  • [1]. inase-activated receptors differentially modulate in vitro invasion of human pancreatic adenocarcinoma PANC-1 cells in correlation with changes in the expression of CDC42 protein. Pancreas. 2014 Jan; 43(1): 10.1097/MPA.0b013e31829f0b81.