QL9 (QLSPFPFDL) is a high-affinity alloantigen for the 2C T cell receptor (TCR).

TCR^[1]

Mouse T cell clone 2C recognizes two different major histocompatibility (MHC) ligands, the self MHC K^b and the allogeneic MHC L^d. Two distinct peptides, SIY (SIYRYYGL) and QL9 (QLSPFPFDL), act as strong and specific agonists when bind to K^b and L^d, respectively. QL9 binding to MHC L^d is influenced by the majority of peptide side chains, distributed across the entire length of the peptide. Findings with both systems, but QL9-L^d in particular, suggest that many single-residue substitutions, introduced into peptides to improve their binding to MHC and thus their vaccine potential, could impair T cell reactivity due to their dual impact on TCR binding. T cell activation assays are performed to measure effects of peptide SIY and QL9 residues on T cell function^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

1063.21

C₅₂H₇₄N₁₀O₁₄

159646-83-0

Gln-Leu-Ser-Pro-Phe-Pro-Phe-Asp-Leu

QLSPFPFDL

Room temperature in continental US; may vary elsewhere.

Powder	-80deg;C	2 years
	-20deg;C	1 year
In solvent	-80deg;C	6 months
	-20deg;C	1 month

In Vitro:;

H₂O : 33.33 mg/mL (31.35 mM; Need ultrasonic)

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

• [1]. Speir JA, et al. Structural basis of 2C TCR allorecognition of H-2Ld peptide complexes. Immunity. 1998 May;8(5):553-62.

  [2]. Bowerman NA, et al. Different strategies adopted by K(b) and L(d) to generate T cell specificity directed against their respective bound peptides. J Biol Chem. 2009 Nov 20;284(47):32551-61.

Wild type 2C and high affinity 2C T cell transfectants m67 and m6 are incubated with K^b– or L^d-positive cells and various concentrations of peptide SIY and QL9 alanine variants. T cell activation is measured by assaying for levels of IL-2 release. Briefly, T cell transfectants (7.5×10⁴) are incubated with T2-K^b (7.5×10⁴) or T2-L^d (7.5×10⁴) along with various concentrations of peptide for 20-24 h at 37 °C and 5% CO₂. Supernatant is harvested, and levels of IL-2 are measured in an enzyme-linked immunosorbent assay type format. Results are plotted as percentage of maximal IL-2 release=((A_{450 (sample)}-A_{450(no peptide)})/(Max A_450(sample)-A_{450(no peptide)}))×100; signal obtained from no peptide is similar to that obtained for the null peptides MCMV or OVA. Binding curves are generated in GraphPad Prism by plotting the percentage of maximal IL-2 release against peptide concentration. The concentrations of peptide yielding 50% maximal IL-2 release (SD₅₀) are calculated using non-linear regression (sigmoidal fitting; GraphPad Prism) of the activation curves^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

• [1]. Speir JA, et al. Structural basis of 2C TCR allorecognition of H-2Ld peptide complexes. Immunity. 1998 May;8(5):553-62.

  [2]. Bowerman NA, et al. Different strategies adopted by K(b) and L(d) to generate T cell specificity directed against their respective bound peptides. J Biol Chem. 2009 Nov 20;284(47):32551-61.