Rebaudioside I is a natural non-claoric sweetener isolated from S. rebaudiana Morita^[1].

1129.15

C₅₀H₈₀O₂₈

1220616-34-1

莱鲍迪甙I

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Prakash I, et al. Bioconversion of rebaudioside I from rebaudioside A. Molecules. 2014 Oct 28;19(11):17345-55.

Rebaudioside E is a steviol glycoside isolated from Stevia rebaudiana leaves^[1].

967.01

C₄₄H₇₀O₂₃

63279-14-1

瑞鲍迪甙E

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Varuzhan Abelyan, et al. High-purity Rebaudioside D. US8299224B2.

Rebaudioside N is a minor steviol glycoside isolated from the leaves of Stevia rebaudiana Bertoni^[1].

1275.29

C₅₆H₉₀O₃₂

1220616-46-5

莱鲍迪甙N

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Chaturvedula VS, et al. Isolation, NMR Spectral Analysis and Hydrolysis Studies of a Hepta Pyranosyl Diterpene Glycoside from Stevia rebaudiana Bertoni. Biomolecules. 2013 Sep 30;3(4):733-40.

Rebaudioside J is a diterpene glycoside that can be found in Stevia rebaudiana.

1113.15

C₅₀H₈₀O₂₇

1313049-59-0

瑞鲍迪甙 J

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Shawn Erickson, et al. Compositions comprising rebaudioside j. WO2018044588A1.

Rebaudioside G is the minor constituent isolated from the leaves of Stevia rebaudiana Bertoni, used for sweeteners research^[1].

804.87

C₃₈H₆₀O₁₈

127345-21-5

甜菊糖G苷

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Ibrahim MA, et al. Rebaudiosides R and S, Minor Diterpene Glycosides from the Leaves of Stevia rebaudiana. J Nat Prod. 2016 May 27;79(5):1468-72.

Rebaudioside A is a steviol glycoside, α-glucosidase inhibitor with IC50 of 35.01 μg/ml.can inhibit ATP-sensitive K+-channels. Target: α-glucosidase [1] IC 50: 35.01 ug/mL In vitro: rebaudioside A stimulat the insulin secretion from MIN6 cells in a dose- and glucose-dependent manner. In conclusion, the insulinotropic effect of rebaudioside A is mediated via inhibition of ATP-sensitive K+-channels and requires the presence of high glucose. [2] In vivo: in vivo mouse micronucleus test at doses up to 750 mg/kg bw and an unscheduled DNA synthesis test in rats at doses up to 2000 mg/kg bw, rebaudioside A do not cause any genotoxic effects at any of the doses tested.[3]

967.01

C₄₄H₇₀O₂₃

58543-16-1

莱苞迪甙 A；瑞鲍迪甙 A；甜菊双糖苷 A；A 甙甜菊糖；甜菊双糖甙 A

Room temperature in continental US; may vary elsewhere.

DMSO : ≥ 100 mg/mL (103.41 mM)

* “≥” means soluble, but saturation unknown.

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% saline

  Solubility: ≥ 2.5 mg/mL (2.59 mM); Clear solution

  此方案可获得 ≥ 2.5 mg/mL (2.59 mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

  将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

• 2.

  请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in saline)

  Solubility: ≥ 2.5 mg/mL (2.59 mM); Clear solution

  此方案可获得 ≥ 2.5 mg/mL (2.59 mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

  将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
• 3.

  请依序添加每种溶剂： 10% DMSO    90% corn oil

  Solubility: ≥ 2.5 mg/mL (2.59 mM); Clear solution

  此方案可获得 ≥ 2.5 mg/mL (2.59 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

  以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

• [1]. Adari BR et al. Synthesis of rebaudioside-A by enzymatic transglycosylation of stevioside present in the leaves of Stevia rebaudiana Bertoni. Food Chem. 2016 Jun 1;200:154-8.

  [2]. Abudula R et al. Rebaudioside A directly stimulates insulin secretion from pancreatic beta cells: a glucose-dependent action via inhibition of ATP-sensitive K-channels. Diabetes Obes Metab. 2008 Nov;10(11):1074-85.

  [3]. Williams LD et al. Genotoxicity studies on a high-purity rebaudioside A preparation. Food Chem Toxicol. 2009 Aug;47(8):1831-6.