标签归档：scjag

JAG-1, scrambled(Synonyms: scJag-1)

发表于由

JAG-1, scrambled;(Synonyms: scJag-1)

JAG-1, scrambled (scJag-1) 是 JAG-1 的杂乱序列。JAG-1, scrambled 与 JAG-1 活性片段具有相同的氨基酸组成，但排列顺序是随机打乱的。 JAG-1, scrambled 常用作阴性对照。

JAG-1, scrambledamp;;(Synonyms: scJag-1)

JAG-1, scrambled Chemical Structure

CAS No. : 402941-23-5

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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JAG-1, scrambled 的其他形式现货产品:

JAG-1, scrambled TFA

生物活性

JAG-1, scrambled (scJag-1) is a scrambled sequence of JAG-1 (Jagged-1 protein). JAG-1, scrambled has a random sequence of the amino acids that are the same as the active fragment. JAG-1, scrambled is usually used as a negative control[1].

体外研究
(In Vitro)

In contrast to JAG-1, pretreatment of human pulmonary arterial smooth muscle cells (PASMC) with 50 μM scJag-1 for 30 min has no effect on the transient and plateau components of store-operated Ca2+ entry (SOCE) in comparison to the cells treated with vehicle used to dissolve scJag-1[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

2107.35

Formula

C93H127N25O26S3
CAS 号

402941-23-5
Sequence

Arg-Cys-Gly-Pro-Asp-Cys-Phe-Asp-Asn-Tyr-Gly-Arg-Tyr-Lys-Tyr-Cys-Phe
Sequence Shortening

RCGPDCFDNYGRYKYCF
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Yamamura H, et al. Activation of Notch signaling by short-term treatment with Jagged-1 enhances store-operated Ca(2+) entry in human pulmonary arterial smooth muscle cells. Am J Physiol Cell Physiol. 2014 May 1;306(9):C871-8.