CY5-SE(Synonyms: Cy5 NHS Ester Sulfo-Cyanine5 Succinimidyl Ester)

CY5-SE;(Synonyms: Cy5 NHS Ester; Sulfo-Cyanine5 Succinimidyl Ester) 纯度: ge;98.0%

Cy5-SE (Cy5 NHS Ester) 是一种用于标记肽, 蛋白质, 寡核苷酸的氨基基团的反应染料。 激发波长 (nm): 649, 发射波长 (nm): 670。

CY5-SEamp;;(Synonyms: Cy5 NHS Ester;  Sulfo-Cyanine5 Succinimidyl Ester)

CY5-SE Chemical Structure

CAS No. : 146368-14-1

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ¥1824 In-stock
5 mg ¥1500 In-stock
10 mg ¥2200 In-stock
25 mg ¥4400 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

CY5-SE 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Cy5-SE (Cy5 NHS Ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reaction. This reagent is ideal for very cost-efficient labeling of soluble proteins, as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling. Excitation (nm):649, Emission (nm): 670.

体外研究
(In Vitro)

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Conjugation of SELP Analogues with Cy5-SE.
1. 1 mL of each 100 μg/mL SELP solution in 100 mM sodium bicarbonate buffer (pH 8.3) is mixed with 10 μL of 1.2 mg/mL Cy5 mono NHS-ester in 10% DMSO and incubates for 2 hours on ice.
2. To quench the reaction, 50 μL of 1 M Tris-HCl (pH 8.0) is added to the reaction solution.
3. Reaction mixtures are loaded onto 1.5 mL Sephadex G-25 columns, and Cy5-conjugated SELPs are eluted by centrifugation for 3 minutes at 1050g[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

753.88

Formula

C37H43N3O10S2

CAS 号

146368-14-1

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)

溶解性数据
In Vitro:;

DMSO : 25 mg/mL (33.16 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.3265 mL 6.6324 mL 13.2647 mL
5 mM 0.2653 mL 1.3265 mL 2.6529 mL
10 mM 0.1326 mL 0.6632 mL 1.3265 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (3.32 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.32 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (3.32 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.32 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Jeon HY, et al. Array-Based High-Throughput Analysis of Silk-Elastinlike Protein Polymer Degradation and C-Peptide Release by Proteases. Anal Chem. 2016;88(10):5398-5405.

荧光染料Janelia Fluor® 669, SE(Synonyms: JF669, SE; JF669, NHS)

荧光染料Dye Reagents Janelia Fluor® 669, SE;(Synonyms: JF669, SE; JF669, NHS)

Janelia Fluor® 669, SE (JF669, SE) 是一种红色的荧光染料,可与含巯基的卤代标记配体在温和的条件下 (DIEA, DMF) 反应,一步形成 JF669-HaloTag (Ex = 669 nm; Em = 682 nm)。Janelia Fluor® 产品在美国专利下获得许可,来自 Howard Hughes Medical Institute 的 No.9,933,417,10,018,624,10,161,932 和其他专利。

Janelia Fluor® 669, SE(Synonyms: JF669, SE;  JF669, NHS)

Janelia Fluor® 669, SE Chemical Structure

CAS No. : 2127150-20-1

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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生物活性

Janelia Fluor® 669, SE (JF669, SE), a red fluorescent dye, can be directly reacted with the available thiol-containing HaloTag ligand under mild conditions (DIEA, DMF) to afford a JF669−HaloTag ligand in a single step (Ex = 669 nm; Em = 682 nm)[1]. Janelia Fluor® products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.

体外研究
(In Vitro)

JF669 undergoes rapid conversion to thioether product (<5 min to completion) in organic solvents, where the dye predominantly adopts closed form[1].
JF669 displays comparatively excellent photostability, retaining 97% fluorescence after the same number of cycles[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

693.76

Formula

C34H30F3N3O6SSi

CAS 号

2127150-20-1

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Jonathan B Grimm, et al. General Synthetic Method for Si-Fluoresceins and Si-Rhodamines. ACS Cent Sci. 2017 Sep 27;3(9):975-985.

荧光染料Janelia Fluor® 525, SE(Synonyms: JF525, SE; JF525, NHS)

荧光染料Dye Reagents Janelia Fluor® 525, SE;(Synonyms: JF525, SE; JF525, NHS)

Janelia Fluor® 525, SE (JF525, SE) 是一种黄色的荧光染料 (Ex = 525 nm; Em = 549 nm)。Janelia Fluor® 在美国专利下获得许可,来自 Howard Hughes Medical Institute 的 No.9,933,417,10,018,624,10,161,932 和其他专利。

Janelia Fluor® 525, SE(Synonyms: JF525, SE;  JF525, NHS)

Janelia Fluor® 525, SE Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

Janelia Fluor® 525, SE (JF525, SE) is a yellow fluorescent dye (Ex = 525 nm; Em = 549 nm)[1]. Janelia Fluor® products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.

分子量

623.51

Formula

C31H21F4N3O7

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Jonathan B Grimm, et al. A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat Methods. 2017 Oct;14(10):987-994.

荧光染料5-FAM SE

荧光染料Dye Reagents 5-FAM SE; 纯度: ge;95.0%

5-FAM SE 是一种单一异构体,是一种用于标记肽,蛋白质和核苷酸的荧光标记试剂。5-FAM SE 可以与胺反应,并可以产生稳定的胺结合物。

5-FAM SEamp;;

5-FAM SE Chemical Structure

CAS No. : 92557-80-7

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ¥1422 In-stock
10 mg ¥1293 In-stock
50 mg ¥4278 In-stock
100 mg ¥7812 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

5-FAM SE 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

5-FAM SE is a single isomer, is a fluorescent labeling reagent used for labeling peptides, proteins and nucleotides. 5-FAM SE can react with amines and can yield stable amine conjugates[1][2].

体外研究
(In Vitro)

The capillary electrophoresis (CE)-laser-induced fluorescent (LIF) method with 5-FAM SE (5-Carboxyfluorescein N-succinimidyl ester) derivatization for the analysis of serotonin can be used to quantify luminally released 5-HT with high sensitivity. CFSE is an excellent dye for the derivatization of 5-HT[1].
5-FAM SE (5-Carboxyfluorescein succinimidyl ester) is chosen as the labeling reagent to precapillary derivatize the two marker aminohydroxyphenylalanine (AHP) isomers produced after reductive hydrolysis of pheomelanin with hydriodic acid (HI)[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

473.39

Formula

C25H15NO9

CAS 号

92557-80-7

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

溶解性数据
In Vitro:;

DMSO : 25 mg/mL (52.81 mM; Need ultrasonic and warming)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.1124 mL 10.5621 mL 21.1242 mL
5 mM 0.4225 mL 2.1124 mL 4.2248 mL
10 mM 0.2112 mL 1.0562 mL 2.1124 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Sheng-da Qi, et al. Quantification of luminally released serotonin in rat proximal colon by capillary electrophoresis with laser-induced fluorescence detection. Anal Bioanal Chem. 2009 Apr;393(8):2059-66.

    [2]. Xiao-Ling Zhang, et al. Sensitive determination of pheomelanin after 5-carboxyfluorescein succinimidyl ester precapillary derivatization and micellar electrokinetic capillary chromatography with laser-induced fluorescence detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Jan 1;861(1):136-9.

CY7-SE triethylamine(Synonyms: Sulfo-Cyanine7 Succinimidyl Ester triethylamine)

CY7-SE triethylamine;(Synonyms: Sulfo-Cyanine7 Succinimidyl Ester triethylamine) 纯度: 96.85%

CY7-SE triethylamine (Sulfo-Cyanine7 Succinimidyl Ester triethylamine) 是一种荧光标记试剂 (Ex=700-770 nm,Em=790 nm)。Cyanine 染料可用于标记蛋白质,抗体,肽,寡聚核苷酸等。

CY7-SE triethylamineamp;;(Synonyms: Sulfo-Cyanine7 Succinimidyl Ester triethylamine)

CY7-SE triethylamine Chemical Structure

规格 价格 是否有货 数量
5 mg ¥1200 In-stock
10 mg ¥1850 In-stock
25 mg ¥3900 In-stock
50 mg ¥6500 In-stock
100 mg ¥10500 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

CY7-SE triethylamine 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

CY7-SE triethylamine (Sulfo-Cyanine7 Succinimidyl Ester triethylamine) is a fluorescence labeling agent (Ex=700-770 nm, Em=790 nm). Cyanine dyes are used to label proteins, antibodies, peptides, and oligonucleotides.

分子量

881.11

Formula

C45H60N4O10S2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, sealed storage, away from moisture and light

*该产品在溶液状态不稳定,建议您现用现配,即刻使用。

溶解性数据
In Vitro:;

DMSO : 83.33 mg/mL (94.57 mM; Need ultrasonic)

H2O : 10 mg/mL (11.35 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.1349 mL 5.6747 mL 11.3493 mL
5 mM 0.2270 mL 1.1349 mL 2.2699 mL
10 mM 0.1135 mL 0.5675 mL 1.1349 mL

*

请根据产品在不同溶剂中的溶解度,选择合适的溶剂配制储备液;该产品在溶液状态不稳定,建议您现用现配,即刻使用

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.08 mg/mL (2.36 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (2.36 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (2.36 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (2.36 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
*以上所有助溶剂都可在 MCE 网站选购。

5-TAMRA-SE(Synonyms: 5-TAMRA-NHS ester 5-Carboxytetramethylrhodamine succinimidyl ester)

5-TAMRA-SE;(Synonyms: 5-TAMRA-NHS ester; 5-Carboxytetramethylrhodamine succinimidyl ester) 纯度: 98.13%

5-TAMRA-SE是胺反应的荧光剂,其偶联物产生明亮,pH不敏感的橙红色荧光,具有良好的光稳定性。

5-TAMRA-SEamp;;(Synonyms: 5-TAMRA-NHS ester;  5-Carboxytetramethylrhodamine succinimidyl ester)

5-TAMRA-SE Chemical Structure

CAS No. : 150810-68-7

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ¥1625 In-stock
1 mg ¥600 In-stock
5 mg ¥1400 In-stock
10 mg ¥2000 In-stock
50 mg ¥7500 In-stock
100 mg ; 询价 ;
200 mg ; 询价 ;

* Please select Quantity before adding items.

5-TAMRA-SE 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

5-TAMRA-SE is an amine-reactive fluorescent agent, its conjugates yield bright, pH-insensitive orange-red fluorescence (approximate excitation/emission maxima ~546/579) with good photostability.

分子量

527.52

Formula

C29H25N3O7

CAS 号

150810-68-7

中文名称

5-羧基四甲基罗丹明琥珀酰亚胺酯

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, protect from light, stored under nitrogen

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light, stored under nitrogen)

溶解性数据
In Vitro:;

DMF : 10 mg/mL (18.96 mM; Need ultrasonic)

DMSO : 5 mg/mL (9.48 mM; Need ultrasonic and warming)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.8957 mL 9.4783 mL 18.9566 mL
5 mM 0.3791 mL 1.8957 mL 3.7913 mL
10 mM 0.1896 mL 0.9478 mL 1.8957 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMF ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 1 mg/mL (1.90 mM); Clear solution

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Kierzek R, et al.5′-Amino Pyrene Provides a Sensitive, Nonperturbing Fluorescent Probe of RNA Secondary and Tertiary Structure Formation. J Am Chem Soc (1993) 115:4985-4985

    [2]. Schmidt T, et al. Local Stoichiometries Determined by Counting Individual Molecules. Anal Chem (1996) 68:4397-4397

Cy2-SE (iodine)(Synonyms: Cyanine2 Succinimidyl Ester (iodine))

Cy2-SE (iodine);(Synonyms: Cyanine2 Succinimidyl Ester (iodine)) 纯度: ge;95.0%

CY2-SE (Cyanine2 Succinimidyl Ester) 是一种用于标记肽, 蛋白质, 寡核苷酸的氨基基团的染料。 激发波长 (nm): 492, 发射波长 (nm): 510。

Cy2-SE (iodine)amp;;(Synonyms: Cyanine2 Succinimidyl Ester (iodine))

Cy2-SE (iodine) Chemical Structure

CAS No. : 186205-33-4

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ¥4955 In-stock
5 mg ¥3500 In-stock
10 mg ¥5600 In-stock
25 mg ¥10500 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Cy2-SE (iodine) 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

CY2-SE (Cyanine2 Succinimidyl Ester) is a dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. Excitation (nm):492, Emission (nm): 510.

分子量

643.47

Formula

C29H30IN3O6

CAS 号

186205-33-4

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)

溶解性数据
In Vitro:;

DMSO : ≥ 35 mg/mL (54.39 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.5541 mL 7.7704 mL 15.5407 mL
5 mM 0.3108 mL 1.5541 mL 3.1081 mL
10 mM 0.1554 mL 0.7770 mL 1.5541 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

5(6)-FAM SE(Synonyms: 5(6)-Carboxyfluorescein N-hydroxysuccinimide ester 5(6)-Carboxyfluorescein succinimidyl ester mixed isomers)

5(6)-FAM SE;(Synonyms: 5(6)-Carboxyfluorescein N-hydroxysuccinimide ester; 5(6)-Carboxyfluorescein succinimidyl ester mixed isomers) 纯度: 95.42%

5(6)-FAM SE 是一种绿色荧光染料,广泛应用于蛋白质标记。

5(6)-FAM SEamp;;(Synonyms: 5(6)-Carboxyfluorescein N-hydroxysuccinimide ester;  5(6)-Carboxyfluorescein succinimidyl ester mixed isomers)

5(6)-FAM SE Chemical Structure

CAS No. : 117548-22-8

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ¥500 In-stock
10 mg ¥280 In-stock
50 mg ¥580 In-stock
100 mg ¥860 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

5(6)-FAM SE 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

5(6)-FAM SE is an amine-reactive green fluorescent dye widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine.

分子量

473.39

Formula

C50H30N2O18

CAS 号

117548-22-8

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

溶解性数据
In Vitro:;

DMSO : 125 mg/mL (264.05 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.1124 mL 10.5621 mL 21.1242 mL
5 mM 0.4225 mL 2.1124 mL 4.2248 mL
10 mM 0.2112 mL 1.0562 mL 2.1124 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.08 mg/mL (4.39 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (4.39 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: 2.08 mg/mL (4.39 mM); Suspended solution; Need ultrasonic

    此方案可获得 2.08 mg/mL (4.39 mM) 的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Stafford JM, et al. Accessory factors facilitate the binding of glucocorticoid receptor to the phosphoenolpyruvate carboxykinase gene promoter. J Biol Chem. 2001 Oct 26;276(43):39885-91.

    [2]. Powell RD, et al. Combined fluorescent and gold immunoprobes: reagents and methods for correlative light and electron microscopy. Microsc Res Tech. 1998 Jul 1;42(1):2-12.

Janelia Fluor® 646, SE(Synonyms: JF646, SE JF646, NHS)

Janelia Fluor® 646, SE;(Synonyms: JF646, SE; JF646, NHS)

Janelia Fluor® 646, SE (JF646, SE) 是一种红色荧光染料可用于细胞成像实验中。Janelia Fluor® 产品根据美国专利获得许可,来自 Howard Hughes Medical Institute 的 No.9,933,417,10,018,624,10,161,932 和其他专利。

Janelia Fluor® 646, SEamp;;(Synonyms: JF646, SE;  JF646, NHS)

Janelia Fluor® 646, SE Chemical Structure

CAS No. : 1811539-59-9

规格 是否有货
1 mg 询价

* Please select Quantity before adding items.

生物活性

Janelia Fluor® 646, SE (JF646, SE) is a red fluorescent dye can be used in cellular imaging[1]. Janelia Fluor® products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.

体外研究
(In Vitro)

Excitation maximum=646 nm; emission maximum=664 nm[1].
JF646 can serve as ligand for self-labeling tag (such as HaloTag)[1].
Janelia Fluor® 646, SE (JF646, SE) is modified by JF646. JF646, a red fluorescent dye, is photostable, membrane-permeable, has a high extinction coefficient[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

593.70

Formula

C33H31N3O6Si

CAS 号

1811539-59-9

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Grimm JB, et al. A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat Methods. 2015;12(3):244-250. doi:10.1038/nmeth.3256

    [2]. Basu S, et al. FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells. Nat Commun. 2018;9(1):2520. Published 2018 Jun 28.

6-FAM SE

6-FAM SE; 纯度: ge;95.0%

6-FAM SE是羧基荧光素的同分异构体。6-FAM SE主要被用于核酸测序和标记核苷酸。

6-FAM SEamp;;

6-FAM SE Chemical Structure

CAS No. : 92557-81-8

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ¥990 In-stock
10 mg ¥900 In-stock
50 mg ¥2900 In-stock
100 mg ¥4900 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

6-FAM SE 相关产品

bull;相关化合物库:

  • Bioactive Compound Library

生物活性

6-FAM SE is another isomer of carboxyfluorescein. 6-FAM, SE is mainly used in sequencing of nucleic acids and labeling nucleotides.

分子量

473.39

Formula

C25H15NO9

CAS 号

92557-81-8

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

溶解性数据
In Vitro:;

DMSO : 50 mg/mL (105.62 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.1124 mL 10.5621 mL 21.1242 mL
5 mM 0.4225 mL 2.1124 mL 4.2248 mL
10 mM 0.2112 mL 1.0562 mL 2.1124 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (5.28 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (5.28 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (5.28 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (5.28 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
*以上所有助溶剂都可在 MCE 网站选购。