Stachyose, a kind of oligosaccharides, act as a hypoglycemic agent[1].
IC50 Target
Human Endogenous Metabolite
;
体内研究 (In Vivo)
Stachyose (oral gavage; 200 mg/kg; 17 days) combines with Berberine (100 mg/kg) produces better hypoglycemic effects than berberine alone. stachyose alone does not decrease the glycaemia of diabetic db/db mice. Additonally, the expression of mucin‐2 is decreased in the Sta + BBR and Sta groups, with a decrease of 2.4% and 2.1%, respectively; and no change is found in the BBR group[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
666.58
Formula
C24H42O21
CAS 号
470-55-3
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献
[1]. Cai-Na Li, et al. Berberine combined with stachyose induces better glycometabolism than berberine alone through modulating gut microbiota and fecal metabolomics in diabetic mice. Phytother Res. 2020 May;34(5):1166-1174.
Stachyose hydrate act as a prebiotic to enhance the growth and activity of beneficial bacteria. Stachyose hydrate exhibit a hypoglycemic effect, and improve inflammation through modulating gut microbiota[1].
IC50 Target
Human Endogenous Metabolite
;
Formula
C24H42O21.xH2O
CAS 号
54261-98-2
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献
[1]. Cai-Na Li, et al. Berberine combined with stachyose induces better glycometabolism than berberine alone through modulating gut microbiota and fecal metabolomics in diabetic mice. Phytother Res. 2020 May;34(5):1166-1174.
Stachyose tetrahydrate, a functional oligosaccharide, acts as a prebiotic. Stachyose tetrahydrate can prevent indirectly colon cancer cell growth by promoting the proliferation of probiotics or producing beneficial materials in the intestine[1][2].
体外研究 (In Vitro)
Stachyose tetrahydrate inhibits Caco-2 cell proliferation and induces apoptosis in a dose-dependent manner[1]. Stachyose highly promotes proliferation of lactic acid bacteria (LAB) by inducing LAB to produce more α-galactosidase to hydrolyze stachyose[2].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
738.64
Formula
C24H50O25
CAS 号
10094-58-3
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
4deg;C, sealed storage, away from moisture and light
*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:;
DMSO : 250 mg/mL (338.46 mM; Need ultrasonic)
H2O : 100 mg/mL (135.38 mM; Need ultrasonic)
配制储备液
浓度溶剂体积质量
1 mg
5 mg
10 mg
1 mM
1.3538 mL
6.7692 mL
13.5384 mL
5 mM
0.2708 mL
1.3538 mL
2.7077 mL
10 mM
0.1354 mL
0.6769 mL
1.3538 mL
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:
[1]. Huang G, et al. Stachyose-induced apoptosis of Caco-2 cells via the caspase-dependent mitochondrial pathway. Food Funct. 2015;6(3):765-771.
[2]. Pan Q, et al. The Proliferation Mechanism of Lactobacillus plantarum RB1 Stimulated by Stachyose. Curr Microbiol. 2017 Jun;74(6):732-738.
Animal Administration [1]
Mice[1]
Male Kunming mice in each group (n=8) are administered by intragastric gavage with saline, stachyose (250 mg/kg bw), genistein (100 mg/kg bw), and stachyose (50, 250, and 500 mg/kg bw) together with genistein (100 mg/kg bw) for 4 consecutive weeks, respectively, and then their urine, feces, blood, gut, and liver are collected[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献
[1]. Huang G, et al. Stachyose-induced apoptosis of Caco-2 cells via the caspase-dependent mitochondrial pathway. Food Funct. 2015;6(3):765-771.
[2]. Pan Q, et al. The Proliferation Mechanism of Lactobacillus plantarum RB1 Stimulated by Stachyose. Curr Microbiol. 2017 Jun;74(6):732-738.