标签归档：stock

FHZ

发表于由

FHZ; 纯度: 98.17%

FHZ 是一个荧光探针。

FHZamp;;

FHZ Chemical Structure

CAS No. : 1883737-63-0

规格 价格 是否有货 数量
5 mg ￥2200 In-stock
10 mg ￥3700 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

FHZ 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

FHZ is a fluorescent probe.

体外研究
(In Vitro)

After loaded with probe FHZ and treated with HClO and H2O2/EDTA-Fe2+ in order, HeLa cells display the bright fluorescences from both cyan and green channels. FHZ can give out two different fluorescent signals in the presence of both •OH and HClO, suggesting the synchronous discrimination of •OH and HClO by a dual-channel detection model with two exciting wavelengths. Probe FHZ shows very high specificity to the detections of •OH and HClO with the excitations at 410 and 490 nm, respectively. The probe FHZ can efficiently enter the cellular mitochondria and exhibit the differentiable/visual capabilities to the endogenous •OH and HClO by the dual fluorescent responses[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究
(In Vivo)

Probe FHZ can rapidly be absorbed into the blood circulation system from the zebrafish intestine, and spread out whole zebrafish tissues, and keep its stability in the blood, organs and tissues in the absence of ROS. The probe can keep its stability in biological environments and only selectively react with •OH and HClO species[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

478.49

Formula

C26H26N2O7
CAS 号

1883737-63-0
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20deg;C 3 years
4deg;C 2 years
In solvent -80deg;C 6 months
-20deg;C 1 month
参考文献

  • [1]. Zhang R, et al. Real-Time Discrimination and Versatile Profiling of Spontaneous Reactive Oxygen Species in Living Organisms with a Single Fluorescent Probe. J Am Chem Soc. 2016 Mar 23;138(11):3769-78.
Cell Assay
[1]

HeLa cells and RAW264.7 macrophages are cultured in high glucose Dulbecco’s modified Eagle’s medium (DMEM) supplied with 10% fetal bovine serum (FBS) and 1% antibotics (penicillin and streptomycin) at 37°C in humidified incubator containing 5% CO2. The cells are seeded into glass-bottomed dishes and cultured for 24 h. Subsequently, the cells are incubated with FHZ for 30 min at 37°C and then washed with PBS buffer three times. Each treatment of cells with H2O2, EDTA-Fe2+, HClO or scavengers kept 30 min[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[1]

Wild type zebrafish is used in this study. Seven-day old fertilized zebrafish embryos are cultured in 50 μM FHZ for 30 min, and then the zebrafish embryos are transferred to fresh water. A FHZ-loaded zebrafish is fixed under confocal microscope using 2% agarose gel to keep its living state for fluorescent imaging. In order to observe the release of •OH in fresh wound, the ventral fin of the FHZ-loaded zebrafish is carefully cut a small wound using a blade. After raised for 20 min in water, the wound of injured zebrafish is imaged using confocal microscope[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Zhang R, et al. Real-Time Discrimination and Versatile Profiling of Spontaneous Reactive Oxygen Species in Living Organisms with a Single Fluorescent Probe. J Am Chem Soc. 2016 Mar 23;138(11):3769-78.

Angoroside C(Synonyms: 安格洛甙C)

发表于由

天然产物 糖类和糖苷 Saccharides and Glycosides

Angoroside C;(Synonyms: 安格洛甙C) 纯度: 99.93%

Angoroside C 是从Radix Scrophulariae 中提取到的一种苯丙苷，有益于心室重构。

Angoroside C(Synonyms: 安格洛甙C)

Angoroside C Chemical Structure

CAS No. : 115909-22-3

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ￥1209 In-stock
5 mg ￥800 In-stock
10 mg ￥1400 In-stock
20 mg ￥2300 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Angoroside C 相关产品

bull;相关化合物库:

  • Natural Product Library Plus
  • Bioactive Compound Library Plus
  • Natural Product Library
  • Glycoside Compound Library
  • Anti-Cardiovascular Disease Compound Library
  • Medicine Food Homology Compound Library
  • Phenols Library
  • Traditional Chinese Medicine Monomer Library

生物活性

Angoroside C, a phenylpropanoid glycoside isolated from Radix Scrophulariae, has beneficial effects against ventricular remodeling[1].

体内研究
(In Vivo)

Angoroside C has beneficial effects against ventricular remodeling. The mechanism is likely to be related to decreasing the level of Ang Ⅱ, attenuating the mRNA expressions of ET-1 and TGF-β1[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

784.75

Formula

C36H48O19
CAS 号

115909-22-3
中文名称

安格洛甙C
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)
溶解性数据
In Vitro:;

DMSO : 125 mg/mL (159.29 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.2743 mL 6.3715 mL 12.7429 mL
5 mM 0.2549 mL 1.2743 mL 2.5486 mL
10 mM 0.1274 mL 0.6371 mL 1.2743 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.08 mg/mL (2.65 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (2.65 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (2.65 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (2.65 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
  • 3.

    请依序添加每种溶剂：;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.08 mg/mL (2.65 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (2.65 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Gu WL, et al. The effect of angoroside C on pressure overload-induced ventricular remodeling in rats. Phytomedicine. 2015 Jul 15;22(7-8):705-12.

Nociceptin(Synonyms: 孤菲肽 Orphanin FQ)

发表于由

Nociceptin;(Synonyms: 孤菲肽; Orphanin FQ) 纯度: 99.83%

Nociceptin 是一种十七肽，为 nociceptin 受体的内源性配体，具有缓解疼痛的作用。

Nociceptinamp;;(Synonyms: 孤菲肽; Orphanin FQ)

Nociceptin Chemical Structure

CAS No. : 170713-75-4

规格 价格 是否有货 数量
1 mg ￥700 In-stock
5 mg ￥2800 In-stock
10 mg ￥5100 In-stock
25 mg ￥11000 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Nociceptin 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • GPCR/G Protein Compound Library
  • Neuronal Signaling Compound Library
  • Pyroptosis Compound Library
  • Neurotransmitter Receptor Compound Library
  • Targeted Diversity Library
  • Peptide Library

生物活性

Nociceptin, a heptadecapeptide, is the endogenous ligand of the nociceptin receptor, acting as a potent anti-analgesic.

体外研究
(In Vitro)

Nociceptin (1 μg/mL) significantly prevents LPS (10 ng/mL)-stimulated cell migration whereas it is ineffective when added alone. Nociceptin (1 nM-10 μM) elicits a concentration-dependent blockade of LPS-mediated cell migration, with a maximal effect at 1 and 10 μM. Nociceptin counteracts LPS-induced elevation of IL-1β mRNA levels. Nociceptin (1 μM) and NNC 55-0396 induce apoptotic cell death in U87 cells. Nociceptin (1 μM) counteracts LPS-induced [Ca2+]i increase in U87 cells via β-arrestin 2. Nociceptin counteracts the LPS-induced phosphorylation of PKC and ERK in U87 cells. Nociceptin inhibits the LPS-mediated transcriptional activation of NF-kB and AP-1 reporter genes[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1809.04

Formula

C79H129N27O22
CAS 号

170713-75-4
Sequence

Phe-Gly-Gly-Phe-Thr-Gly-Ala-Arg-Lys-Ser-Ala-Arg-Lys-Leu-Ala-Asn-Gln
Sequence Shortening

FGGFTGARKSARKLANQ
中文名称

孤菲肽；痛敏肽；孤啡肽
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

DMSO : 50 mg/mL (27.64 mM; Need ultrasonic)

H2O : ≥ 50 mg/mL (27.64 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.5528 mL 2.7639 mL 5.5278 mL
5 mM 0.1106 mL 0.5528 mL 1.1056 mL
10 mM 0.0553 mL 0.2764 mL 0.5528 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (1.38 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (1.38 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (1.38 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (1.38 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
  • 3.

    请依序添加每种溶剂：;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.5 mg/mL (1.38 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (1.38 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Bedini A, et al. Nociceptin/orphanin FQ antagonizes lipopolysaccharide-stimulated proliferation, migration and inflammatory signaling in human glioblastoma U87 cells. Biochem Pharmacol. 2017 Sep 15;140:89-104.
Cell Assay
[1]

Cell proliferation assay is carried out in the assay. U87 cells are plated on 12-well plate and treated for 24 h maintained in cell culture medium containing 10% fetal bovine serum. Five hours before the end of the treatments, [methyl-3H] Thymidine (50 nM final concentration) is added to serum-free cell culture medium and the plate is incubated at 37°C. Thereafter, medium is removed and cells are washed twice with PBS. 200 μL of PBS is added to each well, the cells are scraped off and centrifuged at 13,000g for 3 min at 4°C; supernatants are then discarded, pellets resuspended in 500 μL of cold trichloroacetic acid (10% w/v), incubated on ice for 20 min and centrifuged at 13,000g for 3 min at 4°C. The obtained supernatant is then discarded, pellet suspended in 500 μL of cold methanol and centrifuged at 3 min for 13,000g at 4°C. After that, the pellet is suspended in 200 μL of NaOH 1 N and heated at 55°C for 10 min. Samples are then neutralized with 200 μL of HCl 1 N and 350 μL of the labeled DNA incubated in counting vials with 4 mL of Filter Count scintillation liquid. Vials are vortexed and incubated overnight at room temperature and the radioactivity is determined by liquid scintillation spectrometry.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Bedini A, et al. Nociceptin/orphanin FQ antagonizes lipopolysaccharide-stimulated proliferation, migration and inflammatory signaling in human glioblastoma U87 cells. Biochem Pharmacol. 2017 Sep 15;140:89-104.

(+)-Gallocatechin

发表于由

天然产物 黄酮类 Flavonoids

(+)-Gallocatechin  纯度: 99.68%

(+)-Gallocatechin 是一种来源绿茶的多酚类化合物，具有抗癌活性。

(+)-Gallocatechin

(+)-Gallocatechin Chemical Structure

CAS No. : 970-73-0

规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥500 In-stock
5 mg ￥600 In-stock
10 mg ￥1000 In-stock
20 mg ￥1800 In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

(+)-Gallocatechin 相关产品

•相关化合物库:

  • Natural Product Library Plus
  • Bioactive Compound Library Plus
  • Natural Product Library
  • Anti-Cancer Compound Library
  • Phenols Library
  • Traditional Chinese Medicine Monomer Library
  • Flavonoids Library
  • Food-Sourced Compound Library

生物活性

(+)-Gallocatechin is a polyphenol compound from green tea, possesses anticancer activity[1].

分子量

306.27

Formula

C15H14O7
CAS 号

970-73-0
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶解性数据
In Vitro: 

DMSO : 100 mg/mL (326.51 mM; Need ultrasonic)

H2O : 3.33 mg/mL (10.87 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.2651 mL 16.3255 mL 32.6509 mL
5 mM 0.6530 mL 3.2651 mL 6.5302 mL
10 mM 0.3265 mL 1.6325 mL 3.2651 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (8.16 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (8.16 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (8.16 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (8.16 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
  • 3.

    请依序添加每种溶剂： 10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (8.16 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (8.16 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 Shanghai Jinpan Biotech Co Ltd 网站选购。
参考文献

  • [1]. Du GJ, et al. Epigallocatechin Gallate (EGCG) is the most effective cancer chemopreventive polyphenol in green tea. Nutrients. 2012 Nov 8;4(11):1679-91.

DMA

发表于由

DMA; 纯度: 99.64%

DMA 是一种荧光化合物 (λex=340 nm, λem=478 nm)。

DMAamp;;

DMA Chemical Structure

CAS No. : 188860-26-6

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ￥660 In-stock
5 mg ￥600 In-stock
10 mg ￥900 In-stock
50 mg ￥3600 In-stock
100 mg ￥6600 询价
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

DMA 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Anti-Cancer Compound Library

生物活性

DMA is a fluorescent compound (λex=340 nm, λem=478 nm).

IC50 Target

IC50: 3.4 μM (HeLa cell), 5.3 μM (MCF7 cell)[1]
体外研究
(In Vitro)

The newly synthesized bisbenzimidazole derivatives DMA (6c) is evaluated for their cytotoxicity against human tumor cell lines, which are cervix carcinoma cell line (HeLa), breast carcinoma cell line (MCF7) and brain glioma cell line (U87) in comparison to Hoechst. In case of MCF7, the IC50 is observed at 5.3 μM for DMA. The IC50 determined in the case of HeLa is 3.4 μM for DMA[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial

分子量

468.55

Formula

C27H28N6O2
CAS 号

188860-26-6
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20deg;C 3 years
4deg;C 2 years
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

DMSO : ≥ 54 mg/mL (115.25 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.1342 mL 10.6712 mL 21.3424 mL
5 mM 0.4268 mL 2.1342 mL 4.2685 mL
10 mM 0.2134 mL 1.0671 mL 2.1342 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Singh M, et al. Synthesis and biological activity of novel inhibitors of topoisomerase I: 2-aryl-substituted 2-bis-1H-benzimidazoles. Eur J Med Chem. 2011 Feb;46(2):659-69.
Cell Assay

Various human tumor cells (U87, HeLa and MCF7) are maintained as monolayer at 37°C in 5% CO2 using DMEM medium. Approximately 3000-8000 cells/well are seeded in 96-well plates containing 200 μL of medium and incubated for 24 h. The culture medium is replaced by fresh medium containing 1, 10, 50, 100 μM of DMA (6c) and incubated for 24, 48 and 72 h. The cell viability is determined by the MTT assay. The light absorbance is measured using a microplate reader[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Singh M, et al. Synthesis and biological activity of novel inhibitors of topoisomerase I: 2-aryl-substituted 2-bis-1H-benzimidazoles. Eur J Med Chem. 2011 Feb;46(2):659-69.

Phyllanthin

发表于由

天然产物 天然产物苯丙素类 Phenylpropanoids

Phyllanthin  纯度: 99.96%

Phyllanthin 是 Phyllanthus amarus 的主要生物活性木脂素成分。Phyllanthin 具有很高的抗氧化和保肝作用。

Phyllanthin

Phyllanthin Chemical Structure

CAS No. : 10351-88-9

规格 价格 是否有货 数量
1 mg ￥1000 In-stock
5 mg ￥2500 In-stock
10 mg   询价  
50 mg   询价  

* Please select Quantity before adding items.

Phyllanthin 相关产品

•相关化合物库:

  • Natural Product Library Plus
  • Bioactive Compound Library Plus
  • Natural Product Library
  • Traditional Chinese Medicine Monomer Library

生物活性

Phyllanthin is a major bioactive lignan component of Phyllanthus amarus. Phyllanthin exhibits high antioxidative and hepatoprotective properties[1].

分子量

418.52

Formula

C24H34O6
CAS 号

10351-88-9
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶解性数据
In Vitro: 

DMSO : 100 mg/mL (238.94 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.3894 mL 11.9469 mL 23.8937 mL
5 mM 0.4779 mL 2.3894 mL 4.7787 mL
10 mM 0.2389 mL 1.1947 mL 2.3894 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (5.97 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (5.97 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂： 10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (5.97 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (5.97 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Hanh ND, et al. Physicochemical characterization of phyllanthin from Phyllanthus amarus Schum. et Thonn. Drug Dev Ind Pharm. 2014 Jun;40(6):793-802.

Tectoridin

发表于由

天然产物 黄酮类 Flavonoids

Tectoridin  纯度: 99.57%

Tectoridin 是一种从 Maackia amurensis 分离的异黄酮。Tectoridin 是一种植物雌激素，可激活雌激素和甲状腺激素受体。Tectoridin 通过 ER 依赖性基因组途径和 GPR30 依赖性非基因组途径发挥雌激素作用。

Tectoridin

Tectoridin Chemical Structure

CAS No. : 611-40-5

规格 价格 是否有货 数量
5 mg ￥500 In-stock
10 mg ￥800 In-stock
20 mg ￥1400 In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

Tectoridin 相关产品

•相关化合物库:

  • Natural Product Library Plus
  • Bioactive Compound Library Plus
  • Natural Product Library
  • Anti-Cancer Compound Library
  • Glycoside Compound Library
  • Phenols Library
  • Traditional Chinese Medicine Monomer Library
  • Flavonoids Library

生物活性

Tectoridin is a isoflavone isolated from Maackia amurensis. Tectoridin is a phytoestrogen and activates estrogen and thyroid hormone receptors. Tectoridin exerts the estrogenic effects via ER-dependent genomic pathway and GPR30-dependent nongenomic pathway[1][2].

体外研究
(In Vitro)

Tectoridin scarcely binds to ER alpha as compared to 17beta-estradiol and genistein[2].
Tectoridin induceds potent estrogenic effects, namely recovery of the population of cells in the S-phase after serum starvation, transactivation of the estrogen response element, and induction of MCF-7 cell proliferation[2].
Tectoridin induces estrogenic effect, and this effect is severely abrogated by treatment with U0126 ( MEK1/2 inhibitor). Tectoridin promotes phosphorylation of ERK1/2, but does not affect phosphorylation of ER alpha at Ser (118). It also increases cellular accumulation of cAMP[2].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

462.40

Formula

C22H22O11
CAS 号

611-40-5
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶解性数据
In Vitro: 

DMSO : 250 mg/mL (540.66 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.1626 mL 10.8131 mL 21.6263 mL
5 mM 0.4325 mL 2.1626 mL 4.3253 mL
10 mM 0.2163 mL 1.0813 mL 2.1626 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (4.50 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (4.50 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (4.50 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (4.50 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
  • 3.

    请依序添加每种溶剂： 10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (4.50 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (4.50 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 Shanghai Jinpan Biotech Co Ltd 网站选购。
参考文献

  • [1]. Shim M, et al. Tectoridin from Maackia amurensis modulates both estrogen and thyroid receptors.Phytomedicine. 2014 Apr 15;21(5):602-6.

    [2]. Kang K, et al. Tectoridin, a poor ligand of estrogen receptor alpha, exerts its estrogenic effects via an ERK-dependent pathway.Mol Cells. 2009 Mar 31;27(3):351-7.

TMB(Synonyms: BM blue Sure Blue TMB)

发表于由

TMB;(Synonyms: BM blue; Sure Blue TMB) 纯度: 99.83%

TMB (BM blue) 是免疫组化和ELISA的显色底物。

TMBamp;;(Synonyms: BM blue; Sure Blue TMB)

TMB Chemical Structure

CAS No. : 54827-17-7

规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg) ; Apply now ;
10;mM;*;1 mL in DMSO ￥550 In-stock
500 mg ￥400 In-stock
1 g ￥500 In-stock
5 g ￥1100 In-stock
10 g ; 询价 ;
50 g ; 询价 ;

* Please select Quantity before adding items.

TMB 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

TMB (BM blue) is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA).

分子量

240.34

Formula

C16H20N2
CAS 号

54827-17-7
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20deg;C 3 years
4deg;C 2 years
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

DMSO : 100 mg/mL (416.08 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 4.1608 mL 20.8039 mL 41.6077 mL
5 mM 0.8322 mL 4.1608 mL 8.3215 mL
10 mM 0.4161 mL 2.0804 mL 4.1608 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

Nattokinase

发表于由

Nattokinase;

Nattokinase 是一种有效的纤溶酶，可通过直接水解纤维蛋白和纤溶酶底物来分解血凝块。Nattokinase 可用于研究心血管疾病。

Nattokinaseamp;;

Nattokinase Chemical Structure

CAS No. : 133876-92-3

规格 价格 是否有货 数量
5 mg ￥900 In-stock
10 mg ￥1500 In-stock
50 mg ￥4000 In-stock
100 mg ￥6800 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Nattokinase 相关产品

bull;相关化合物库:

  • Drug Repurposing Compound Library Plus
  • Clinical Compound Library Plus
  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Nattokinase is a potent fibrinolytic enzyme. Nattokinase can break down blood clots by directly hydrolyzing fibrin and plasmin substrate. Nattokinase can be used for the research of cardiovascular diseases[1].

体外研究
(In Vitro)

Nattokinase dissolves 94% blood clot at ten minutes and degrades the fibrin clot after two hours of incubation[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究
(In Vivo)

Nattokinase (150-250 mg/kg; oral gavage twice daily for 2 days) exhibits fibrinolytic activity and dissolves blood clots in vivo[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Sprague-Dawley (SD) rats (180-220 g) are injected κ-Carrageenan[3]
Dosage: 150, 250 mg/kg
Administration: Oral gavage twice daily for 2 days
Result: Increased the plasma concentration of fibrin/fibrinogen degradation products (FDPs) and D-Dimer in a dose-dependent manner.
Decreased the fraction of the vessel cross section occupied by thrombosis.

Clinical Trial

CAS 号

133876-92-3
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

H2O : 100 mg/mL (Need ultrasonic)
参考文献

  • [1]. Weng Y, et, al. Nattokinase: An Oral Antithrombotic Agent for the Prevention of Cardiovascular Disease. Int J Mol Sci. 2017 Feb 28; 18(3):523.

    [2]. Chandrasekaran SD, et, al. Exploring the In Vitro Thrombolytic Activity of Nattokinase From a New Strain Pseudomonas aeruginosa CMSS. Jundishapur J Microbiol. 2015 Oct 26; 8(10): e23567.

    [3]. Xu J, et, al. Thrombolytic effects in vivo of nattokinase in a carrageenan-induced rat model of thrombosis. Acta Haematol. 2014; 132(2): 247-53.

DAOS

发表于由

生化分析试剂 Biochemical Assay Reagents
DAOS; 纯度: 99.43%

DAOS是新型 Trinder’s 试剂，是高水溶性苯胺衍生物，被广泛用于诊断检测和生化试验。

DAOS

DAOS Chemical Structure

CAS No. : 83777-30-4

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ￥550 In-stock
100 mg ￥500 In-stock
250 mg ￥850 In-stock
500 mg ￥1400 询价
1 g ; 询价 ;
5 g ; 询价 ;

* Please select Quantity before adding items.

DAOS 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

DAOS, a Trinder’s reagent, is a novel highly water-soluble aniline derivative; are widely used in diagnostic tests and biochemical tests.

分子量

341.36

Formula

C13H20NNaO6S
CAS 号

83777-30-4
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, sealed storage, away from moisture

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)
溶解性数据
In Vitro:;

DMSO : ≥ 36 mg/mL (105.46 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.9295 mL 14.6473 mL 29.2946 mL
5 mM 0.5859 mL 2.9295 mL 5.8589 mL
10 mM 0.2929 mL 1.4647 mL 2.9295 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.08 mg/mL (6.09 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (6.09 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (6.09 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (6.09 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
  • 3.

    请依序添加每种溶剂：;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.08 mg/mL (6.09 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (6.09 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。