标签归档：substrate

Cathepsin D and E FRET Substrate acetate

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Cathepsin D and E FRET Substrate acetate; 纯度: 99.06%

Cathepsin D and E FRET Substrate acetate 为 cathepins D 和 E 的荧光底物 (fluorogenic substrate)，而不是 B、H 或 I 的荧光底物，它的裂解发生在 Phe-Phe 酰胺键处。Cathepsin D and E FRET Substrate 是用于组织蛋白酶 D 和组织蛋白酶 E 的常规检测和机制研究的有用工具。(激发波长:328 nm; 发射波长:393 nm)

Cathepsin D and E FRET Substrate acetateamp;;

Cathepsin D and E FRET Substrate acetate Chemical Structure

规格 价格 是否有货 数量
5 mg ￥2800 In-stock
10 mg ￥4500 In-stock
25 mg ￥8900 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Cathepsin D and E FRET Substrate acetate 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Cathepsin D and E FRET Substrate acetate is a fluorogenic substrate for cathepsins D and E and not for B, H or L. The cleavage occurs at the Phe-Phe amide bond resul. Cathepsin D and E FRET Substrate is a valuable tool for routine assays and for mechanistic studies on cathepsins E and D[1].

分子量

1816.15

Formula

C87H126N22O21
Sequence

MOCAc-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-{Lys(Dnp)}-{D-Arg}-NH2
Sequence Shortening

MOCAc-GKPILFFRL-{Lys(Dnp)}-{D-Arg}-NH2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)
溶解性数据
In Vitro:;

H2O : 10 mg/mL (5.51 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.5506 mL 2.7531 mL 5.5062 mL
5 mM 0.1101 mL 0.5506 mL 1.1012 mL
10 mM — — —

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Y Yasuda, et al. Characterization of new fluorogenic substrates for the rapid and sensitive assay of cathepsin E and cathepsin D. J Biochem

MBP MAPK Substrate

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MBP MAPK Substrate;

MBP MAPK Substrate 可以用作 MAPK 的外源底物。

MBP MAPK Substrateamp;;

MBP MAPK Substrate Chemical Structure

CAS No. : 138028-00-9

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

MBP MAPK Substrate is used as an exogenous substrate for MAPK[1].

分子量

967.09

Formula

C39H70N18O11
CAS 号

138028-00-9
Sequence

Ala-Pro-Arg-Thr-Pro-Gly-Gly-Arg-Arg
Sequence Shortening

APRTPGGRR
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. K C Gause, et al. Effects of phorbol ester on mitogen-activated protein kinase kinase activity in wild-type and phorbol ester-resistant EL4 thymoma cells. J Biol Chem. 1993 Aug 5;268(22):16124-9.

Calcineurin substrate TFA

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Calcineurin substrate TFA; 纯度: 99.57%

Calcineurin substrate (TFA) 是来自 cAMP 依赖性蛋白激酶调节性RII亚基的多肽。Calcineurin substrate (TFA) 可用于钙调神经磷酸酶活力的测定。

Calcineurin substrate TFAamp;;

Calcineurin substrate TFA Chemical Structure

规格 价格 是否有货 数量
500 μg ￥1100 In-stock
1 mg ￥1950 In-stock
5 mg ￥8400 In-stock
10 mg ; 询价 ;
50 mg ; 询价 ;

* Please select Quantity before adding items.

Calcineurin substrate TFA 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Calcineurin substrate (TFA) is a peptide from the regulatory RII subunit of cAMP-dependent protein kinase. Calcineurin substrate (TFA) can be used in the calcineurin activity assay[1].

分子量

2226.37

Formula

C94H151F3N28O31
Sequence

Asp-Leu-Asp-Val-Pro-Ile-Pro-Gly-Arg-Phe-Asp-Arg-Arg-Val-Ser-Val-Ala-Ala-Glu
Sequence Shortening

DLDVPIPGRFDRRVSVAAE
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)
参考文献

  • [1]. Crabtree GR, et al. Calcium, calcineurin, and the control of transcription. J Biol Chem. 2001 Jan 26;276(4):2313-6.

Sakamototide substrate peptide TFA

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Sakamototide substrate peptide TFA; 纯度: 98.79%

Sakamototide substrate peptide TFA 是 AMPK 激酶家族成员的肽底物，可用于激酶活性测定。

Sakamototide substrate peptide TFAamp;;

Sakamototide substrate peptide TFA Chemical Structure

规格 价格 是否有货 数量
10;mM;*;1 mL in Water ￥7140 In-stock
5 mg ￥2200 In-stock
10 mg ￥3500 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Sakamototide substrate peptide TFA 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Kinase Inhibitor Library
  • Peptide Library

生物活性

Sakamototide substrate peptide TFA is a peptide substrate for members of the AMPK family of kinases, used in kinase activity assays[1][2].

分子量

1853.92

Formula

C71H123F3N30O25
Sequence

Ala-Leu-Asn-Arg-Thr-Ser-Ser-Asp-Ser-Ala-Leu-His-Arg-Arg-Arg
Sequence Shortening

ALNRTSSDSALHRRR
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)
溶解性数据
In Vitro:;

H2O : 100 mg/mL (53.94 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.5394 mL 2.6970 mL 5.3940 mL
5 mM 0.1079 mL 0.5394 mL 1.0788 mL
10 mM 0.0539 mL 0.2697 mL 0.5394 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Banerjee S,et al. Characterization of WZ4003 and HTH-01-015 as selective inhibitors of the LKB1-tumour-suppressor-activated NUAK kinases. Biochem J. 2014 Jan 1;457(1):215-25.

    [2]. Banerjee S, et al. Interplay between Polo kinase, LKB1-activated NUAK1 kinase, PP1βMYPT1 phosphatasecomplex and the SCFβTrCP E3 ubiquitin ligase. Biochem J. 2014 Jul 15;461(2):233-45.

DAPK Substrate Peptide TFA

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DAPK Substrate Peptide TFA; 纯度: 99.33%

DAPK Substrate Peptide TFA 是一种合成的死亡相关蛋白激酶 (DAPK) 的肽底物， Km 为 9 μM。

DAPK Substrate Peptide TFAamp;;

DAPK Substrate Peptide TFA Chemical Structure

规格 价格 是否有货 数量
5 mg ￥3500 In-stock
10 mg ￥5800 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

DAPK Substrate Peptide TFA is a synthetic peptide substrate for death associated protein kinase (DAPK), with a Km of 9 μM[1].

分子量

1692.84

Formula

C72H116F3N25O19
Sequence

Lys-Lys-Arg-Pro-Gln-Arg-Arg-Tyr-Ser-Asn-Val-Phe
Sequence Shortening

KKRPQRRYSNVF
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)
参考文献

  • [1]. Velentza AV. A protein kinase associated with apoptosis and tumor suppression: structure, activity, and discovery of peptide substrates. J Biol Chem. 2001;276(42):38956-38965.

Abl Cytosolic Substrate

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Abl Cytosolic Substrate;

Abl Cytosolic Substrate是Abl 酪氨酸激酶的底物。Abl 蛋白酪氨酸激酶是v-AbI蛋白酪氨酸激酶的一种截断形式，是Abelson小鼠白血病病毒Gag-Abl融合蛋白的一个伙伴。

Abl Cytosolic Substrateamp;;

Abl Cytosolic Substrate Chemical Structure

CAS No. : 168202-46-8

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

Abl Cytosolic Substrate is a substrate for Abelson tyrosine kinase (Abl ). Abl Protein Tyrosine Kinase (AbI) is a truncated form of the v-AbI Protein Tyrosine Kinase, a partner in the Gag-Abl fusion protein of the Abelson murine leukemia virus[1].

分子量

1336.58

Formula

C64H101N15O16
CAS 号

168202-46-8
Sequence

Glu-Ala-Ile-Tyr-Ala-Ala-Pro-Phe-Ala-Lys-Lys-Lys
Sequence Shortening

EAIYAAPFAKKK
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Buchdunger E, et al. Inhibition of the Abl protein-tyrosine kinase in vitro and in vivo by a 2-phenylaminopyrimidine derivative. Cancer Res. 1996 Jan 1;56(1):100-4.

Calcineurin substrate(Synonyms: 钙调神经磷酸酶底物)

发表于由

Calcineurin substrate;(Synonyms: 钙调神经磷酸酶底物)

Calcineurin substrate 是来自 cAMP 依赖性蛋白激酶调节性 RII 亚基的多肽。Calcineurin substrate 可用于钙调神经磷酸酶活力的测定。

Calcineurin substrateamp;;(Synonyms: 钙调神经磷酸酶底物)

Calcineurin substrate Chemical Structure

CAS No. : 113873-67-9

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Calcineurin substrate 的其他形式现货产品:

Calcineurin substrate TFA

生物活性

Calcineurin substrate is a peptide from the regulatory RII subunit of cAMP-dependent protein kinase. It can be used in the calcineurin activity assay.

体外研究
(In Vitro)

Calcineurin (PP2B), a serine/threonine phosphatase controlled by cellular calcium, has been implicated in a wide variety of biological responses including lymphocyte activation, neuronal and muscle development, neurite outgrowth, and morphogenesis of vertebrate heart valves[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

2112.35

Formula

C92H150N28O29
CAS 号

113873-67-9
Sequence

Asp-Leu-Asp-Val-Pro-Ile-Pro-Gly-Arg-Phe-Asp-Arg-Arg-Val-Ser-Val-Ala-Ala-Glu
Sequence Shortening

DLDVPIPGRFDRRVSVAAE
中文名称

钙调神经磷酸酶底物
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Crabtree GR, et al. Calcium, calcineurin, and the control of transcription. J Biol Chem. 2001 Jan 26;276(4):2313-6.

HIV Protease Substrate 1

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HIV Protease Substrate 1;

HIV Protease Substrate 1 是一种外源性 HIV 蛋白酶底物，可用于研究 HIV 蛋白酶的酶活性。

HIV Protease Substrate 1amp;;

HIV Protease Substrate 1 Chemical Structure

CAS No. : 223769-59-3

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

HIV Protease Substrate 1, a fiuorogenic HIV protease substrate, can be used to study enzymatic activity of HIV protease[1].

体外研究
(In Vitro)

HIV Protease Substrate 1 is a synthetic peptide sequence that contains the cleavage site (Tyr-Pro) for HIV protease as well as two covalently modified amino acids for the detection of cleavage[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

2017.27

Formula

C92H133N27O23S
CAS 号

223769-59-3
Sequence

Arg-{Glu(EDANS)}-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-Lys{(DABCYL)}-Arg
Sequence Shortening

R{Glu(EDANS)}SQNYPIVQ{Lys(DABCYL)}R
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Kapewangolo P, et al. Anti-HIV Activity of Ocimum labiatum Extract and Isolated Pheophytin-a. Molecules. 2017;22(11):1763. Published 2017 Nov 6.

Cathepsin D and E FRET Substrate

发表于由

Cathepsin D and E FRET Substrate;

Cathepsin D and E FRET Substrate 为 cathepins D 和 E 的荧光底物 (fluorogenic substrate)，而不是 B、H 或 I 的荧光底物，它的裂解发生在 Phe-Phe 酰胺键处。Cathepsin D and E FRET Substrate 是用于组织蛋白酶 D 和组织蛋白酶 E 的常规检测和机制研究的有用工具。(激发波长:328 nm; 发射波长:393 nm)

Cathepsin D and E FRET Substrateamp;;

Cathepsin D and E FRET Substrate Chemical Structure

CAS No. : 839730-93-7

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Cathepsin D and E FRET Substrate 的其他形式现货产品:

Cathepsin D and E FRET Substrate acetate

生物活性

Cathepsin D and E FRET Substrate is a fluorogenic substrate for cathepsins D and E and not for B, H or L. The cleavage occurs at the Phe-Phe amide bond resul. Cathepsin D and E FRET Substrate is a valuable tool for routine assays and for mechanistic studies on cathepsins E and D[1].

分子量

1756.10

Formula

C85H122N22O19
CAS 号

839730-93-7
Sequence

MOCAc-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-{Lys(Dnp)}-{D-Arg}-NH2
Sequence Shortening

MOCAc-GKPILFFRL-{Lys(Dnp)}-{D-Arg}-NH2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Y Yasuda, et al. Characterization of new fluorogenic substrates for the rapid and sensitive assay of cathepsin E and cathepsin D. J Biochem

Akt/SKG Substrate Peptide TFA

发表于由

Akt/SKG Substrate Peptide TFA;

Akt/SKG Substrate Peptide TFA 是一种合成的 Akt/PKB 底物肽，不会被 p70S6K 或 MAPK1 磷酸化。

Akt/SKG Substrate Peptide TFAamp;;

Akt/SKG Substrate Peptide TFA Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

Akt/SKG Substrate Peptide TFA is a synthetic peptide suitable as a substrate for Akt/PKB, which is not phosphorylated by p70S6K or MAPK1[1].

分子量

931.96

Formula

C38H60F3N13O11
Sequence

Arg-Pro-Arg-Ala-Ala-Thr-Phe
Sequence Shortening

RPRAATF
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.