DAPK Substrate Peptide TFA

DAPK Substrate Peptide TFA; 纯度: 99.33%

DAPK Substrate Peptide TFA 是一种合成的死亡相关蛋白激酶 (DAPK) 的肽底物, Km 为 9 μM。

DAPK Substrate Peptide TFAamp;;

DAPK Substrate Peptide TFA Chemical Structure

规格 价格 是否有货 数量
5 mg ¥3500 In-stock
10 mg ¥5800 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

DAPK Substrate Peptide TFA is a synthetic peptide substrate for death associated protein kinase (DAPK), with a Km of 9 μM[1].

分子量

1692.84

Formula

C72H116F3N25O19

Sequence

Lys-Lys-Arg-Pro-Gln-Arg-Arg-Tyr-Ser-Asn-Val-Phe

Sequence Shortening

KKRPQRRYSNVF

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)

参考文献
  • [1]. Velentza AV. A protein kinase associated with apoptosis and tumor suppression: structure, activity, and discovery of peptide substrates. J Biol Chem. 2001;276(42):38956-38965.

Abl Cytosolic Substrate

Abl Cytosolic Substrate;

Abl Cytosolic Substrate是Abl 酪氨酸激酶的底物。Abl 蛋白酪氨酸激酶是v-AbI蛋白酪氨酸激酶的一种截断形式,是Abelson小鼠白血病病毒Gag-Abl融合蛋白的一个伙伴。

Abl Cytosolic Substrateamp;;

Abl Cytosolic Substrate Chemical Structure

CAS No. : 168202-46-8

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

Abl Cytosolic Substrate is a substrate for Abelson tyrosine kinase (Abl ). Abl Protein Tyrosine Kinase (AbI) is a truncated form of the v-AbI Protein Tyrosine Kinase, a partner in the Gag-Abl fusion protein of the Abelson murine leukemia virus[1].

分子量

1336.58

Formula

C64H101N15O16

CAS 号

168202-46-8

Sequence

Glu-Ala-Ile-Tyr-Ala-Ala-Pro-Phe-Ala-Lys-Lys-Lys

Sequence Shortening

EAIYAAPFAKKK

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献
  • [1]. Buchdunger E, et al. Inhibition of the Abl protein-tyrosine kinase in vitro and in vivo by a 2-phenylaminopyrimidine derivative. Cancer Res. 1996 Jan 1;56(1):100-4.

Calcineurin substrate(Synonyms: 钙调神经磷酸酶底物)

Calcineurin substrate;(Synonyms: 钙调神经磷酸酶底物)

Calcineurin substrate 是来自 cAMP 依赖性蛋白激酶调节性 RII 亚基的多肽。Calcineurin substrate 可用于钙调神经磷酸酶活力的测定。

Calcineurin substrateamp;;(Synonyms: 钙调神经磷酸酶底物)

Calcineurin substrate Chemical Structure

CAS No. : 113873-67-9

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Calcineurin substrate 的其他形式现货产品:

Calcineurin substrate TFA

生物活性

Calcineurin substrate is a peptide from the regulatory RII subunit of cAMP-dependent protein kinase. It can be used in the calcineurin activity assay.

体外研究
(In Vitro)

Calcineurin (PP2B), a serine/threonine phosphatase controlled by cellular calcium, has been implicated in a wide variety of biological responses including lymphocyte activation, neuronal and muscle development, neurite outgrowth, and morphogenesis of vertebrate heart valves[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

2112.35

Formula

C92H150N28O29

CAS 号

113873-67-9

Sequence

Asp-Leu-Asp-Val-Pro-Ile-Pro-Gly-Arg-Phe-Asp-Arg-Arg-Val-Ser-Val-Ala-Ala-Glu

Sequence Shortening

DLDVPIPGRFDRRVSVAAE

中文名称

钙调神经磷酸酶底物

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Crabtree GR, et al. Calcium, calcineurin, and the control of transcription. J Biol Chem. 2001 Jan 26;276(4):2313-6.

HIV Protease Substrate 1

HIV Protease Substrate 1;

HIV Protease Substrate 1 是一种外源性 HIV 蛋白酶底物,可用于研究 HIV 蛋白酶的酶活性。

HIV Protease Substrate 1amp;;

HIV Protease Substrate 1 Chemical Structure

CAS No. : 223769-59-3

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

HIV Protease Substrate 1, a fiuorogenic HIV protease substrate, can be used to study enzymatic activity of HIV protease[1].

体外研究
(In Vitro)

HIV Protease Substrate 1 is a synthetic peptide sequence that contains the cleavage site (Tyr-Pro) for HIV protease as well as two covalently modified amino acids for the detection of cleavage[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

2017.27

Formula

C92H133N27O23S

CAS 号

223769-59-3

Sequence

Arg-{Glu(EDANS)}-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-Lys{(DABCYL)}-Arg

Sequence Shortening

R{Glu(EDANS)}SQNYPIVQ{Lys(DABCYL)}R

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Kapewangolo P, et al. Anti-HIV Activity of Ocimum labiatum Extract and Isolated Pheophytin-a. Molecules. 2017;22(11):1763. Published 2017 Nov 6.

Cathepsin D and E FRET Substrate

Cathepsin D and E FRET Substrate;

Cathepsin D and E FRET Substrate 为 cathepins D 和 E 的荧光底物 (fluorogenic substrate),而不是 B、H 或 I 的荧光底物,它的裂解发生在 Phe-Phe 酰胺键处。Cathepsin D and E FRET Substrate 是用于组织蛋白酶 D 和组织蛋白酶 E 的常规检测和机制研究的有用工具。(激发波长:328 nm; 发射波长:393 nm)

Cathepsin D and E FRET Substrateamp;;

Cathepsin D and E FRET Substrate Chemical Structure

CAS No. : 839730-93-7

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Cathepsin D and E FRET Substrate 的其他形式现货产品:

Cathepsin D and E FRET Substrate acetate

生物活性

Cathepsin D and E FRET Substrate is a fluorogenic substrate for cathepsins D and E and not for B, H or L. The cleavage occurs at the Phe-Phe amide bond resul. Cathepsin D and E FRET Substrate is a valuable tool for routine assays and for mechanistic studies on cathepsins E and D[1].

分子量

1756.10

Formula

C85H122N22O19

CAS 号

839730-93-7

Sequence

MOCAc-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-{Lys(Dnp)}-{D-Arg}-NH2

Sequence Shortening

MOCAc-GKPILFFRL-{Lys(Dnp)}-{D-Arg}-NH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Y Yasuda, et al. Characterization of new fluorogenic substrates for the rapid and sensitive assay of cathepsin E and cathepsin D. J Biochem

Akt/SKG Substrate Peptide TFA

Akt/SKG Substrate Peptide TFA;

Akt/SKG Substrate Peptide TFA 是一种合成的 Akt/PKB 底物肽,不会被 p70S6K 或 MAPK1 磷酸化。

Akt/SKG Substrate Peptide TFAamp;;

Akt/SKG Substrate Peptide TFA Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

Akt/SKG Substrate Peptide TFA is a synthetic peptide suitable as a substrate for Akt/PKB, which is not phosphorylated by p70S6K or MAPK1[1].

分子量

931.96

Formula

C38H60F3N13O11

Sequence

Arg-Pro-Arg-Ala-Ala-Thr-Phe

Sequence Shortening

RPRAATF

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.

[pThr3]-CDK5 Substrate

[pThr3]-CDK5 Substrate;

[pThr3]-CDK5 Substrate 是 Thr3 磷酸化的 CDK5 底物。[pThr3]-CDK5 Substrate 来自组蛋白 H1,位于组蛋白 H1 上和 CDK5 作用的位点。CDK5 Substrate 被 CDK5 磷酸化为 [pThr3]-CDK5 Substrate 的 Km 为 6 μM。

[pThr3]-CDK5 Substrateamp;;

[pThr3]-CDK5 Substrate Chemical Structure

CAS No. : 1670273-47-8

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

[pThr3]-CDK5 Substrate is an effective Phospho-Thr3CDK5 Substrate. [pThr3]-CDK5 Substrate is derived from the sequence of the histone H1 peptide that docks in the active site of CDK5. [pThr3]-CDK5 Substrate is phosphorylated by CDK5 with a Km value of 6 µM[1].

分子量

1218.43

Formula

C53H100N15O15P

CAS 号

1670273-47-8

Sequence

Pro-Lys-{pTHR}-Pro-Lys-Lys-Ala-Lys-Lys-Leu

Sequence Shortening

PK-{pTHR}-PKKAKKL

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献
  • [1]. Beaudette KN, et al. Substrate specificity characterization of a cdc2-like protein kinase purified from bovine brain. J Biol Chem. 1993 Oct 5;268(28):20825-30.

DAPK Substrate Peptide

DAPK Substrate Peptide;

DAPK Substrate Peptide 是一种合成的死亡相关蛋白激酶 (DAPK) 的肽底物, Km 为 9 μM。

DAPK Substrate Peptideamp;;

DAPK Substrate Peptide Chemical Structure

CAS No. : 386769-53-5

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

DAPK Substrate Peptide 的其他形式现货产品:

DAPK Substrate Peptide TFA

生物活性

DAPK Substrate Peptide is a synthetic peptide substrate for death associated protein kinase (DAPK), with a Km of 9 μM[1].

分子量

1578.82

Formula

FK2NPSVY

CAS 号

386769-53-5

Sequence

Lys-Lys-Arg-Pro-Gln-Arg-Arg-Tyr-Ser-Asn-Val-Phe

Sequence Shortening

KKRPQRRYSNVF

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Velentza AV. A protein kinase associated with apoptosis and tumor suppression: structure, activity, and discovery of peptide substrates. J Biol Chem. 2001;276(42):38956-38965.

Bacterial Sortase Substrate III, Abz/DNP

Bacterial Sortase Substrate III, Abz/DNP;

Bacterial Sortase Substrate III, Abz/DNP 是一种内部淬灭的荧光肽底物。金黄色葡萄球菌转肽酶分选酶A (SrtA) 与 Bacterial Sortase Substrate III, Abz/DNP 反应,裂解并催化苏氨酸的羧基与细胞壁交联桥的氨基之间形成酰胺键。可以在 Ex/Em=320 nm/420 nm 下检测该底物的切割。

Bacterial Sortase Substrate III, Abz/DNPamp;;

Bacterial Sortase Substrate III, Abz/DNP Chemical Structure

规格 价格 是否有货
1 mg ¥1750 询问价格 货期
5 mg ¥5250 询问价格 货期

* Please select Quantity before adding items.

Bacterial Sortase Substrate III, Abz/DNP 的其他形式现货产品:

Bacterial Sortase Substrate III, Abz/DNP TFA

生物活性

Bacterial Sortase Substrate III, Abz/DNP is an internally quenched fluorescent peptide substrate. Staphylococcus aureus transpeptidase sortase A (SrtA) reacts with its native substrate Bacterial Sortase Substrate III, Abz/DNP, cleaving it and catalyzing the formation of an amide bond between the carboxyl group of threonine and the amino group of cell-wall crossbridges. Cleavage of this substrate can be monitored at Ex/Em=320 nm/420 nm.

分子量

928.00

Formula

C41H57N11O14

Sequence Shortening

Abz-LPETG-K(Dnp)-NH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献
  • [1]. Zhang J, et al. Antiinfective therapy with a small molecule inhibitor of Staphylococcus aureus sortase. Proc Natl Acad Sci U S A. 2014 Sep 16;111(37):13517-22.

[pThr3]-CDK5 Substrate TFA

[pThr3]-CDK5 Substrate TFA;

[pThr3]-CDK5 Substrate TFA 是 Thr3 磷酸化的 CDK5 底物。[pThr3]-CDK5 Substrate 来自组蛋白 H1,位于组蛋白 H1 上和 CDK5 作用的位点。CDK5 Substrate 被 CDK5 磷酸化为 [pThr3]-CDK5 Substrate 的 Km 为 6 μM。

[pThr3]-CDK5 Substrate TFAamp;;

[pThr3]-CDK5 Substrate TFA Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

[pThr3]-CDK5 Substrate TFA is an effective Phospho-Thr3CDK5 Substrate. [pThr3]-CDK5 Substrate is derived from the sequence of the histone H1 peptide that docks in the active site of CDK5. [pThr3]-CDK5 Substrate is phosphorylated by CDK5 with a Km value of 6 µM[1].

分子量

1332.45

Formula

C55H101F3N15O17P

Sequence

Pro-Lys-{pTHR}-Pro-Lys-Lys-Ala-Lys-Lys-Leu

Sequence Shortening

PK-{pTHR}-PKKAKKL

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献
  • [1]. Beaudette KN, et al. Substrate specificity characterization of a cdc2-like protein kinase purified from bovine brain. J Biol Chem. 1993 Oct 5;268(28):20825-30.