标签归档：sulfo

Sulfo-SMCC sodium

发表于由

生化分析试剂 Biochemical Assay Reagents
Sulfo-SMCC sodium; 纯度: 97.24%

Sulfo-SMCC sodium 是一种常用的异双官能交联剂，是不可降解的 ADC linker，其带有N-羟基琥珀酰亚胺 (NHS) 酯和马来酰亚胺基团，分别与伯胺和巯基反应。

Sulfo-SMCC sodium

Sulfo-SMCC sodium Chemical Structure

CAS No. : 92921-24-9

规格 价格 是否有货 数量
10 mg ￥500 In-stock
50 mg ￥1300 In-stock
100 mg ￥2300 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

Sulfo-SMCC sodium is a commonly used hetero-bifunctional, noncleavable ADC crosslinker bearing N-hydroxysuccinimide (NHS) ester and maleimide groups to react with primary amines and sulfhydryl groups, respectively.

IC50 Target

Non-cleavable

;

体外研究
(In Vitro)

The crosslinker Sulfo-SMCC consists of a maleimide and an N-hydroxysuccinimide ester group to bind to sulfhydryl groups and primary amines, respectively. Sulfo-SMCC inhibits the end-to-end annealing of stabilized Microtubules (MTs). MTs are treated with 250 μM Sulfo-SMCC, and imaged after incubation for 0 h, 6 h, and 24 h. MTs treated with Sulfo-SMCC shows a constant mean length, independent of the incubation time[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

436.37

Formula

C16H17N2NaO9S
CAS 号

92921-24-9
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:;

DMSO : 125 mg/mL (286.45 mM; Need ultrasonic)

H2O : 5 mg/mL (11.46 mM; ultrasonic and warming and heat to 60°C)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.2916 mL 11.4582 mL 22.9163 mL
5 mM 0.4583 mL 2.2916 mL 4.5833 mL
10 mM 0.2292 mL 1.1458 mL 2.2916 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.08 mg/mL (4.77 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (4.77 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (4.77 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (4.77 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
  • 3.

    请依序添加每种溶剂：;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.08 mg/mL (4.77 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (4.77 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Prabhune M, et al. Sulfo-SMCC Prevents Annealing of Taxol-Stabilized Microtubules In Vitro. PLoS One. 2016 Aug 25;11(8):e0161623.
Kinase Assay
[1]

Labeled and unlabeled porcine tubulin powders are used. Sulfo-SMCC is used. MTs are polymerized to a final concentration of 2 mg/mL in 80 mM PIPES buffer (pH 6.8) containing 10 μM taxol, 2 mM MgCl2, 0.5 mM EGTA, and 1 mM GTP. A mixture of rhodamine-labeled tubulin and unlabeled tubulin (1:5) is used in the time-dependent measurements. In these experiments, polymerized MTs are divided in 2 groups: control and Sulfo-SMCC (250 μM) treated. Both samples are imaged after diluting 1:5 and incubating for 0 h (within 45 minutes after polymerization), 6 h, and 24 h[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Prabhune M, et al. Sulfo-SMCC Prevents Annealing of Taxol-Stabilized Microtubules In Vitro. PLoS One. 2016 Aug 25;11(8):e0161623.

荧光染料Cy5-DBCO(Synonyms: DBCO-Sulfo-Cy5)

发表于由

荧光染料Dye Reagents Cy5-DBCO;(Synonyms: DBCO-Sulfo-Cy5)

Cy5-DBCO (DBCO-Sulfo-Cy5) 是一个近红外 (NIR) 红色荧光染料 λabs 和 λem 值分别为  646 nm 和 670 nm。Cy5-DBCO (DBCO-Sulfo-Cy5) 不适合对通透性细胞的胞内成分进行染色，可能会表现出较高的背景。

Cy5-DBCOamp;;(Synonyms: DBCO-Sulfo-Cy5)

Cy5-DBCO Chemical Structure

CAS No. : 1564286-24-3

规格 价格 是否有货
1 mg ￥6500 询问价格 货期
5 mg ￥14500 询问价格 货期

* Please select Quantity before adding items.

生物活性

Cy5-DBCO (DBCO-Sulfo-Cy5) is a near-infrared (NIR) red fluorescent dye with λabs and λem of  646 nm and 670 nm, respectively. Cy5-DBCO (DBCO-Sulfo-Cy5) is not suitable for staining intracellular components of permeabilezed cell, it may exhibits a high background.

IC50 Target

Red fluorescent dye
体外研究
(In Vitro)

Cy5-DBCO (DBCO-Sulfo-Cy5) reacts with azides via a copper-free “click chemistry” reaction and forms a stable triazole and does not need Cu-catalyst or elevated temperatures.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1009.22

Formula

C52H56N4O11S3
CAS 号

1564286-24-3
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20deg;C 3 years
In solvent -80deg;C 6 months
-20deg;C 1 month

荧光染料Cy5-DBCO(Synonyms: DBCO-Sulfo-Cy5)

发表于由

荧光染料Dye Reagents Cy5-DBCO;(Synonyms: DBCO-Sulfo-Cy5)

Cy5-DBCO (DBCO-Sulfo-Cy5) 是一个近红外 (NIR) 红色荧光染料 λabs 和 λem 值分别为  646 nm 和 670 nm。Cy5-DBCO (DBCO-Sulfo-Cy5) 不适合对通透性细胞的胞内成分进行染色，可能会表现出较高的背景。

Cy5-DBCO(Synonyms: DBCO-Sulfo-Cy5)

Cy5-DBCO Chemical Structure

CAS No. : 1564286-24-3

规格 价格 是否有货
1 mg ￥6500 询问价格 货期
5 mg ￥14500 询问价格 货期

* Please select Quantity before adding items.

生物活性

Cy5-DBCO (DBCO-Sulfo-Cy5) is a near-infrared (NIR) red fluorescent dye with λabs and λem of  646 nm and 670 nm, respectively. Cy5-DBCO (DBCO-Sulfo-Cy5) is not suitable for staining intracellular components of permeabilezed cell, it may exhibits a high background.

IC50 Target

Red fluorescent dye
体外研究
(In Vitro)

Cy5-DBCO (DBCO-Sulfo-Cy5) reacts with azides via a copper-free “click chemistry” reaction and forms a stable triazole and does not need Cu-catalyst or elevated temperatures.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1009.22

Formula

C52H56N4O11S3
CAS 号

1564286-24-3
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20deg;C 3 years
In solvent -80deg;C 6 months
-20deg;C 1 month

CY5(Synonyms: Sulfo-Cyanine5)

发表于由

CY5;(Synonyms: Sulfo-Cyanine5) 纯度: 99.66%

Cy5 (Sulfo-Cyanine5) 是一种用于标记肽, 蛋白质, 寡核苷酸的氨基基团的反应染料。 激发波长 (nm): 649, 发射波长 (nm): 670。

CY5amp;;(Synonyms: Sulfo-Cyanine5)

CY5 Chemical Structure

CAS No. : 146368-11-8

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ￥1878 In-stock
5 mg ￥1300 In-stock
10 mg ￥2000 In-stock
25 mg ￥4200 In-stock
50 mg ￥6700 In-stock
100 mg ; 询价 ;
200 mg ; 询价 ;

* Please select Quantity before adding items.

CY5 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Cy5 (Sulfo-Cyanine5) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reaction. This reagent is ideal for very cost-efficient labeling of soluble proteins, as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling. Excitation (nm):649, Emission (nm): 670.

分子量

656.81

Formula

C33H40N2O8S2
CAS 号

146368-11-8
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)
溶解性数据
In Vitro:;

DMSO : 250 mg/mL (380.63 mM; Need ultrasonic)

H2O : 25 mg/mL (38.06 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.5225 mL 7.6126 mL 15.2251 mL
5 mM 0.3045 mL 1.5225 mL 3.0450 mL
10 mM 0.1523 mL 0.7613 mL 1.5225 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.08 mg/mL (3.17 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (3.17 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (3.17 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (3.17 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
*以上所有助溶剂都可在 MCE 网站选购。

荧光染料CY5-YNE(Synonyms: Sulfo-Cyanine5-alkyne)

发表于由

荧光染料Dye Reagents CY5-YNE;(Synonyms: Sulfo-Cyanine5-alkyne) 纯度: ge;95.0%

CY5-YNE (Sulfo-Cyanine5-alkyne) 是一种用于标记肽, 蛋白质, 寡核苷酸的氨基基团的反应染料。

CY5-YNEamp;;(Synonyms: Sulfo-Cyanine5-alkyne)

CY5-YNE Chemical Structure

CAS No. : 1345823-20-2

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ￥5343 In-stock
5 mg ￥3500 In-stock
10 mg ￥5600 In-stock
25 mg ￥10500 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

CY5-YNE 相关产品

bull;相关化合物库:

  • Covalent Screening Library Plus
  • Bioactive Compound Library Plus
  • Covalent Screening Library

生物活性

CY5-YNE (Sulfo-Cyanine5-alkyne) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides.

分子量

693.87

Formula

C36H43N3O7S2
CAS 号

1345823-20-2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)
溶解性数据
In Vitro:;

DMSO : ≥ 43 mg/mL (61.97 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.4412 mL 7.2060 mL 14.4119 mL
5 mM 0.2882 mL 1.4412 mL 2.8824 mL
10 mM 0.1441 mL 0.7206 mL 1.4412 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

荧光染料DBCO-Cy3(Synonyms: DBCO-Sulfo-Cy3)

发表于由

荧光染料Dye Reagents DBCO-Cy3;(Synonyms: DBCO-Sulfo-Cy3) 纯度: 96.50%

DBCO-Cy3 (DBCO-Sulfo-Cy3) 是 Cyanine3 fluorophore 的衍生物，是一种 pH (4-10) 不敏感的橙红色荧光染料 (orange fluorescent dye)，激发波长和发射波长分别为 555 nm 和 580 nm。Sulfo-Cy3 DBCO 反应动力学快，稳定性好，可用于多种标准荧光仪器。

DBCO-Cy3amp;;(Synonyms: DBCO-Sulfo-Cy3)

DBCO-Cy3 Chemical Structure

CAS No. : 1782950-79-1

规格 价格 是否有货 数量
5 mg ￥6000 In-stock
10 mg ￥9600 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

DBCO-Cy3 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

DBCO-Cy3 (DBCO-Sulfo-Cy3) is the derivative of Cyanine3 fluorophore, a pH insensitive from pH (4-10) orange fluorescent dye with excitation maximum 555 nm and emission maximum of 580 nm. DBCO-Cy3 has fast reaction kinetics and good stability, and is productive to use in many standard fluorescent instrumentations.

IC50 Target

Orange fluorescent dye
体外研究
(In Vitro)

DBCO-Cy3 exhibits less background than TAMRA and most other commonly used fluorescent dyes.
DBCO-Cy3 can be conjugated to antibodies, peptides, and proteins, it can be excited using the 532 nm or 555 nm laser line  and visualized with TRITC filter sets.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

983.18

Formula

C50H54N4O11S3
CAS 号

1782950-79-1
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20deg;C 3 years
4deg;C 2 years
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

DMSO : 125 mg/mL (127.14 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.0171 mL 5.0855 mL 10.1711 mL
5 mM 0.2034 mL 1.0171 mL 2.0342 mL
10 mM 0.1017 mL 0.5086 mL 1.0171 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

Sulfo-NHS-SS-Biotin sodium

发表于由

Sulfo-NHS-SS-Biotin sodium; 纯度: ge;95.0%

Sulfo-NHS-SS-biotin 是一种长链可裂解和细胞无渗透的胺类活性生物素化 (biotinylation) 试剂。Sulfo-NHS-SS-biotin 可用于细胞表面蛋白 (cell-surface protein) 的标记和纯化。

Sulfo-NHS-SS-Biotin sodiumamp;;

Sulfo-NHS-SS-Biotin sodium Chemical Structure

CAS No. : 325143-98-4

规格 价格 是否有货
10 mg ￥1700 询问价格 货期
50 mg ￥5000 询问价格 货期
100 mg ￥8000 询问价格 货期

* Please select Quantity before adding items.

Sulfo-NHS-SS-Biotin sodium 相关产品

bull;相关化合物库:

  • Covalent Screening Library
  • Covalent Screening Library Plus
  • Bioactive Compound Library Plus

生物活性

Sulfo-NHS-SS-biotin is a long-chain cleavable and cell-impermeant amine-reactive biotinylation reagent. Sulfo-NHS-SS-biotin can be used for the labeling and purifying of cell-surface protein[1].

IC50 Target

IC50: cell-surface protein[1]
体外研究
(In Vitro)

Sulfo-NHS-SS-biotin is a cell-surface-labeling reagent, it is a negatively charged reagent and does not permeate cell membranes[1].
Sulfo-NHS-SS-biotin reacts with primary amines (NH2), such as lysine side-chains, or the amino-termini of polypeptides[1].
Sulfo-NHS-SS-biotin has a sulfonate group and prevents it from permeating cell membranes. Thr group’s cleavable spacer arm enables initially biotinylated proteins to be released from streptavidin affinity columns[1].
Sulfo-NHS-SS-biotin (1 mg/ml; 15 min) is applied in cells in monolayer culture (washed by ice-cold PBS). The biotinylation reactions are terminated with 100 mM glycine in PBS. After washing with PBS, cell extracts are prepared in RIPA buffer with protease inhibitor cocktail (HY-K0010). Biotinylated membrane proteins are precipitated with streptavidin-sepharose and proteins are eluted with SDS sample buffer[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

606.69

Formula

C19H27N4NaO9S4
CAS 号

325143-98-4
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

-20deg;C, sealed storage, away from moisture

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)
溶解性数据
In Vitro:;

DMSO : 125 mg/mL (206.04 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.6483 mL 8.2414 mL 16.4829 mL
5 mM 0.3297 mL 1.6483 mL 3.2966 mL
10 mM 0.1648 mL 0.8241 mL 1.6483 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.08 mg/mL (3.43 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (3.43 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (3.43 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (3.43 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
  • 3.

    请依序添加每种溶剂：;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.08 mg/mL (3.43 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (3.43 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Chia CP,et al. Phagocytosis in Dictyostelium discoideum is inhibited by antibodies directed primarily against common carbohydrate epitopes of a major cell-surface plasma membrane glycoprotein.Exp Cell Res. 1989 Mar;181(1):11-26.

    [2]. Jo M, et al. Cell signaling by urokinase-type plasminogen activator receptor induces stem cell-like properties in breast cancer cells. Cancer Res. 2010 Nov 1;70(21):8948-58.

CY7(Synonyms: Sulfo-Cyanine7)

发表于由

CY7;(Synonyms: Sulfo-Cyanine7) 纯度: 95.19%

Cy7 (Sulfo-Cyanine7) 是一种荧光标记试剂 (Ex=750 nm，Em=773 nm)。Cyanine 染料可用于标记蛋白质，抗体，肽，寡聚核苷酸等。

CY7amp;;(Synonyms: Sulfo-Cyanine7)

CY7 Chemical Structure

CAS No. : 943298-08-6

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ￥2554 In-stock
5 mg ￥1700 In-stock
10 mg ￥2700 In-stock
25 mg ￥5400 In-stock
50 mg ￥8900 In-stock
100 mg ; 询价 ;
200 mg ; 询价 ;

* Please select Quantity before adding items.

CY7 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Cy7 (Sulfo-Cyanine7) is a fluorescence labeling agent (Ex=750 nm, Em=773 nm). Cyanine dyes are used to label proteins, antibodies, peptides, and oligonucleotides[1].

体外研究
(In Vitro)

Cyanine dyes such as Cy5.5 and Cy7, are excellent near-infrared (NIR) probes, possessing high molar absorptivity, good photostability and strong fluorescence emission[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

682.85

Formula

C35H42N2O8S2
CAS 号

943298-08-6
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)
溶解性数据
In Vitro:;

DMSO : ≥ 33 mg/mL (48.33 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.4645 mL 7.3223 mL 14.6445 mL
5 mM 0.2929 mL 1.4645 mL 2.9289 mL
10 mM 0.1464 mL 0.7322 mL 1.4645 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Lim B, et al. A Unique Recombinant Fluoroprobe Targeting Activated Platelets Allows In Vivo Detection of Arterial Thrombosis and Pulmonary Embolism Using a Novel Three-Dimensional Fluorescence Emission Computed Tomography (FLECT) Technology. Theranostics. 2017 Feb 26;7(5):1047-1061.

CY7-SE(Synonyms: Sulfo-Cyanine7 Succinimidyl Ester)

发表于由

CY7-SE;(Synonyms: Sulfo-Cyanine7 Succinimidyl Ester)

CY7-SE (Sulfo-Cyanine7 Succinimidyl Ester) 是一种荧光标记试剂 (Ex=700-770 nm，Em=790 nm)。Cyanine 染料可用于标记蛋白质，抗体，肽，寡聚核苷酸等。

CY7-SEamp;;(Synonyms: Sulfo-Cyanine7 Succinimidyl Ester)

CY7-SE Chemical Structure

CAS No. : 477908-53-5

规格 价格 是否有货
10;mM;*;1 mL in DMSO ￥2060 询问价格 货期
5 mg ￥1200 询问价格 货期

* Please select Quantity before adding items.

CY7-SE 的其他形式现货产品:

CY7-SE triethylamine

CY7-SE 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

CY7-SE (Sulfo-Cyanine7 Succinimidyl Ester) is a fluorescence labeling agent (Ex=700-770 nm, Em=790 nm). Cyanine dyes are used to label proteins, antibodies, peptides, and oligonucleotides.

分子量

779.92

Formula

C39H45N3O10S2
CAS 号

477908-53-5
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

-20deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:;

DMSO : 25 mg/mL (32.05 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.2822 mL 6.4109 mL 12.8218 mL
5 mM 0.2564 mL 1.2822 mL 2.5644 mL
10 mM 0.1282 mL 0.6411 mL 1.2822 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (3.21 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.21 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (3.21 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.21 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
*以上所有助溶剂都可在 MCE 网站选购。