SV40 T-Ag-derived NLS peptide

SV40 T-Ag-derived NLS peptide;

SV40 T-Ag-derived NLS peptide 是一种核定位信号肽,标记该肽的 DNA 可有效地转移到细胞核中。

SV40 T-Ag-derived NLS peptideamp;;

SV40 T-Ag-derived NLS peptide Chemical Structure

CAS No. : 105425-98-7

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

SV40 T-Ag-derived NLS peptide is a nuclear localization signal DNA tagged to this peptide efficiently translocates into the cell nucleus[1].

分子量

1490.77

Formula

C66H111N19O18S

CAS 号

105425-98-7

Sequence Shortening

PKKKRKVEDPYC

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献
  • [1]. Benimetskaya L, et al. Protamine-fragment peptides fused to an SV40 nuclear localization signal deliver oligonucleotides that produce antisense effects in prostate and bladder carcinoma cells. Bioconjug Chem. 2002 Mar-Apr;13(2):177-87.

Millipore Ultrafree-CL SV 离心超滤管UFC40SV25

Millipore Ultrafree-CL SV 离心超滤管

简要描述:

型号 : UFC40SV25

特点 : 高回收Durapore(PVDF)和亲水性PTFE膜;五种不同的孔径,从0.1到5.0um;还提供预先灭菌装置;快速过滤和高度重现性;在装有15mL管的固定角度转子中使用。

说明: Ultrafree-CL SV

商标名: Ultrafree

数量/包装: 25

操作注意: 固定角度转子 (15 mL) ,zui大离心力为 5,000 x g

滤膜材质: Hydrophilic PVDF

离心作用: Sample Clarification

色码: 深绿色

zui高操作温度,°C: 50

滤膜类型: 微孔滤膜

长度,mm: 77

滤膜孔径,?m: 5.0

残留体积,?L: 10

直径,mm: 16.3

滤膜代码: SVPP

体积,mL: 2

产品名称: Ultrafree-CL SV

过滤面积,cm2: 0.8

无菌: 非无菌

Millipore Ultrafree-CL SV 离心超滤管

技术指标

样品体积
zui大起始样品体积,mL 2
  10
离心力 固定角度转子 (15 mL) ,zui大离心力为 5,000 x g
材料
亲水性 PVDF或亲水性 PTFE
装置  
尺寸
有效膜面积,cm2 0.8
直径,mm 16.3
长度,mm 77

Millipore Ultrafree-CL SV 离心超滤管

应用

  • 从水和某些基于溶剂的样品中去除颗粒和沉淀物
  • 是配合蛋白质和核酸溶液使用的理想选择

SV40 large T antigen NLS

SV40 large T antigen NLS; 纯度: 99.80%

SV40 large T antigen NLS 是一种多肽,来源于 SV40 大 T 抗原 47-55 的氨基酸序列,能够将蛋白运输到细胞核。

SV40 large T antigen NLSamp;;

SV40 large T antigen NLS Chemical Structure

规格 价格 是否有货 数量
1 mg ¥800 In-stock
5 mg ¥3200 In-stock
10 mg ¥5600 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

SV40 large T antigen NLS 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

SV40 large T antigen NLS is from Large T antigen residue 47 to 55, enables protein import into cell nucleus.

体外研究
(In Vitro)

SV40 large tumor-antigen (T-ag) nuclear import is enhanced by the protein kinase CK2 (CK2) site (Ser111Ser112) flanking the nuclear localization sequence (NLS)[1]. T-ag NLS can enhance nuclear import, despite the fact that PK-C has been shown to inhibit nuclear import of proteins such as lamin B2[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1401.70

Formula

C58H104N20O18S

Sequence

Cys-Gly-Gly-Gly-Pro-Lys-Lys-Lys-Arg-Lys-Val-Glu-Asp

Sequence Shortening

CGGGPKKKRKVED

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)

Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献
  • [1]. Xiao CY, et al. Negative charge at the protein kinase CK2 site enhances recognition of the SV40 large T-antigen NLS by importin: effect of conformation. FEBS Lett. 1998 Dec 4;440(3):297-301.

    [2]. Xiao CY, et al. An engineered site for protein kinase C flanking the SV40 large T-antigen NLS confers phorbol ester-inducible nuclear import. FEBS Lett. 1998 Oct 9;436(3):313-7.

PTFE压入二通阀SV21 6φ

PTFE压入二通阀SV21 6φ

  • 产品特性
  • 相关资料
  • Q&A
  • 参考文献

PTFE压入二通阀SV21 6φPTFE压入二通阀SV21 6φ

PTFE压入二通阀SV21 6φ



  该二通阀的主体的材料为氟树脂PTFE,螺帽的材料为氟树脂PFA。耐化学性、耐热性良好。适合用于连接氟树脂管。

 


◆材料


PTFE、PFA

 


◆产品列表

产品编号

产品名称

型号

适用管外径(mm)

孔径(mm)

WEB15508

PTFE压入二通阀

SV21 6 φ

6 φ

4 φ

※主体:PTFE

※螺帽:PFA

※最大使用压力:0.3 MPa

 


◆常见问题解答


Q.产品中是否有使用O型圈?

A.没有。产品中使用了特氟龙制的算盘珠。

 


◆相关产品

产品编号

产品名称

型号

适用管外径(mm)

孔径(mm)

WEB15507

PTFE压入二通阀

SV22 8 φ

8 φ

4 φ

WEB15509

PTFE压入二通阀

SV23 10 φ

10 φ

6 φ

WEB15510

PTFE压入二通阀

SV24 12 φ

12 φ

9 φ

※ 本页面产品仅供研究用,研究以外不可使用。

Millipore密理博 Millex-SV 过滤器

Millipore密理博 Millex-SV 过滤器

Millipore密理博 Millex-SV 过滤器

产品编号:
市场价:¥0.00
会员价:¥0.00
品牌:Millipore 密理博
生产厂家:Millipore密理博

  • 产品概览
  • 技术参数
  • 订购信息
  • 相关资料
  • 相关产品

说明: Millex-SV,5.0 µm,PVDF,25 mm,环氧乙烷灭菌
商标名: Millex
数量/包装: 50
应用: 水溶液的澄清过滤
进口接头: 阴 Luer-Lok 接头
滤膜材质: 亲水性 PVDF
色码: 进/出口均为无色
滤膜商标名: Durapore®
最大进口压力,bar (psi): 5.2 (75)
出口接头: 阳 luer 滑动接头
最高操作温度,°C: 45
处理体积,mL: 100
滤膜孔径,µm: 5.0
可润湿性: 亲水
残留体积,µL: <100
灭菌: 环氧乙烷
直径,mm: 28.6
滤膜直径,mm: 25
高度,mm: 25
产品名称: Millex-SV 过滤器
无菌: 无菌
过滤面积,cm2: 3.9
装置材质: PVC
订购说明: Millex-SV 5.0µm PVDF 25mm EtO 灭菌 50 个/包装

技术指标

  4mm Millex 13mm Millex 25mm Millex
外壳材料 HDPE HDPE PVC
进口接头 阴 Luer-Lok 接头 阴 Luer-Lok 接头 阴 Luer-Lok 接头
出口接头 阶形阳 Luer 滑动接头 阳 luer 滑动接头 阳 luer 滑动接头
过滤面积, cm2 0.1 0.65 3.9
处理体积,mL 1 10 100
残留体积,µL <10 <25 <100
最大进口气压,bar (psi) 14 (200) 7 (100) 5.2 (75)
最大使用温度,°C 45 45 45

millipore代理,millipore

V5-tag Recombinant monoclonal antibody (SV5-P-K) (Mouse Fab Fragment κ) (His-tag) V5-标签重组单克隆抗体(SV5-P-K)(小鼠Fab片段κ)(His-标签) 品牌:Enzo


V5-tag Recombinant monoclonal antibody (SV5-P-K) (Mouse Fab Fragment κ) (His-tag)

V5-标签重组单克隆抗体(SV5-P-K)(小鼠Fab片段κ)(His-标签)

品牌:Enzo
CAS No.:
储存条件:-20℃
纯度:
产品编号

(生产商编号)

等级 规格 运输包装 零售价(RMB) 库存情况 参考值

ENZ-ABS586-0200

200 µg 7,980.00


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动,恕不另行通知,确切详情请联系上海金畔生物科技有限公司。

V5-tag Recombinant monoclonal antibody (SV5-P-K) (Human Fab Fragment κ) (His-tag) V5-标签重组单克隆抗体(SV5-P-K)(人Fab片段κ)(His标签) 品牌:Enzo


V5-tag Recombinant monoclonal antibody (SV5-P-K) (Human Fab Fragment κ) (His-tag)

V5-标签重组单克隆抗体(SV5-P-K)(人Fab片段κ)(His标签)

品牌:Enzo
CAS No.:
储存条件:-20℃
纯度:
产品编号

(生产商编号)

等级 规格 运输包装 零售价(RMB) 库存情况 参考值

ENZ-ABS587-0200

200 µg 7,980.00


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动,恕不另行通知,确切详情请联系上海金畔生物科技有限公司。

V5-tag Recombinant monoclonal antibody (SV5-P-K) (Hamster IgGκ) V5-标签重组单克隆抗体(SV5-P-K)(仓鼠IgGκ) 品牌:Enzo


V5-tag Recombinant monoclonal antibody (SV5-P-K) (Hamster IgGκ)

V5-标签重组单克隆抗体(SV5-P-K)(仓鼠IgGκ)

品牌:Enzo
CAS No.:
储存条件:-20℃
纯度:
产品编号

(生产商编号)

等级 规格 运输包装 零售价(RMB) 库存情况 参考值

ENZ-ABS588-0200

200 µg 7,980.00


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动,恕不另行通知,确切详情请联系上海金畔生物科技有限公司。

V5-tag Recombinant monoclonal antibody (SV5-P-K) (Rabbit IgGκ) V5-标签重组单克隆抗体(SV5-P-K)(兔IgGκ) 品牌:Enzo


V5-tag Recombinant monoclonal antibody (SV5-P-K) (Rabbit IgGκ)

V5-标签重组单克隆抗体(SV5-P-K)(兔IgGκ)

品牌:Enzo
CAS No.:
储存条件:-20℃
纯度:
产品编号

(生产商编号)

等级 规格 运输包装 零售价(RMB) 库存情况 参考值

ENZ-ABS589-0200

200 µg 7,980.00


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动,恕不另行通知,确切详情请联系上海金畔生物科技有限公司。