标签归档：tat

TAT-Gap19 TFA

发表于由

TAT-Gap19 TFA; 纯度: 98.36%

TAT-Gap19 TFA 是一种 Cx 模拟肽，是一种特定的 connexin43 半通道 (Cx43 HC) 抑制剂。TAT-Gap19 TFA 不抑制相应的 Cx43 GJC。TAT-Gap19 TFA 可以透过血脑屏障，减轻小鼠的肝纤维化。

TAT-Gap19 TFAamp;;

TAT-Gap19 TFA Chemical Structure

规格 价格 是否有货 数量
5 mg ￥2200 In-stock
10 mg ￥3700 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

TAT-Gap19 TFA 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

TAT-Gap19 TFA, a Cx mimetic peptide, is a specific connexin43 hemichannel (Cx43 HC) inhibitor. TAT-Gap19 TFA does not inhibits the corresponding Cx43 GJCs. TAT-Gap19 TFA traverses the blood-brain barrier and alleviate liver fibrosis in mice[1][2][3].

体内研究
(In Vivo)

A single injection of TAT-Gap19 TFA (i.v. via the tail vein; 55 mg/kg) produces significant immune signal in the brain 24 h later in 4 months old C57Bl6 male mice[1].
TAT-Gap19 TFA (1 mg/kg/day; an osmotic pump implanted in the peritoneal cavity; Two weeks) shows significantly decreased collagen deposition, as well as lowed amounts of α-SMA-positive cells area in mice subjected to treatment with 100-200 mg thioacetamide (TAA)/kg body weight for eight weeks[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

2817.27

Formula

C121H213F3N46O28
Sequence Shortening

YGRKKRRQRRRKQIEIKKFK
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)
溶解性数据
In Vitro:;

H2O : 100 mg/mL (35.50 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.3550 mL 1.7748 mL 3.5495 mL
5 mM 0.0710 mL 0.3550 mL 0.7099 mL
10 mM 0.0355 mL 0.1775 mL 0.3550 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Verónica Abudara, et al. The connexin43 mimetic peptide Gap19 inhibits hemichannels without altering gap junctional communication in astrocytes. Front Cell Neurosci. 2014 Oct 21;8:306.

    [2]. Sara Crespo Yanguas, et al. TAT-Gap19 and Carbenoxolone Alleviate Liver Fibrosis in Mice. Int J Mol Sci. 2018 Mar 12;19(3):817.

    [3]. Laura Walrave, et al. Inhibition of astroglial connexin43 hemichannels with TAT-Gap19 exerts anticonvulsant effects in rodents. Glia. 2018 Aug;66(8):1788-1804.

Tat-NR2Baa

发表于由

Tat-NR2Baa; 纯度: 96.26%

Tat-NR2BAA 是 Tat-NR2B9c (HY-P0117) 的对照肽 (control peptide)，没有活性。Tat-NR2BAA 的序列与 Tat-NR2B9c 相似，但在 COOH 末端 tSXV 基序中有一个双点突变，这使得它无法结合 PSD-95。Tat-NR2B9c 是一种可通透细胞膜的多肽，破坏 PSD-95/NMDAR 的结合。

Tat-NR2Baaamp;;

Tat-NR2Baa Chemical Structure

CAS No. : 847829-41-8

规格 价格 是否有货 数量
1 mg ￥2200 In-stock
5 mg ￥5500 In-stock
10 mg ￥9500 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Tat-NR2Baa 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Tat-NR2BAA is the control peptide of Tat-NR2B9c (HY-P0117), inactive. The sequence of Tat-NR2BAA is similar to Tat-NR2B9c, but it has a double-point mutation in the COOH terminal tSXV motif, making it incapable of binding PSD-95. Tat-NR2B9c is a membrane-permeant peptide and disrupts PSD-95/NMDAR binding, correlate with uncoupling NR2B- and/or NR2A-type NMDARs from PSD-95[1][2].

体外研究
(In Vitro)

Tat-NR2BAA (125 ng; 20 mins) does not effects interactions between PSD-95 and NR2B subunits. In contrast, coimmunoprecipitation of PSD-95 with NR2B subunits is markedly decreased in rats pretreated with the disrupting peptide Tat-NR2B9c in lumbar dorsal horn tissue[1].
Tat-NR2Baa (125 ng or 1.25 μg; 20 minutes before collection of lumbar dorsal horn tissue) is the control group of Tat-NR2B9c. Tat-NR2B9c produces a significant and robust reduction of postdischarge, indicating the hyperexcitability of the cell. But Tat-NR2Baa has no effects, even at a dose 100× greater than the active peptide Tat-NR2B9c (HY-P0117)[1].
Tat-NR2Baa (1 μM; pre-treatment 1 hour) is the control group in the Co-IP assay. Tat-NR2B9c (1 μM) disrupts NR2B/PSD95 interaction, and the coupling of NR2B to PSD-95 is more sensitive than NR2A/PSD95 to disruption in hippocampal neurons[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

2474.83

Formula

C103H184N42O29
CAS 号

847829-41-8
Sequence

Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Lys-Leu-Ser-Ser-Ile-Glu-Ala-Asp-Ala
Sequence Shortening

YGRKKRRQRRRKLSSIEADA
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
参考文献

  • [1]. Michelle Aarts, et al. Treatment of Ischemic Brain Damage by Perturbing NMDA Receptor- PSD-95 Protein Interactions. Science

TAT TFA

发表于由

TAT TFA; 纯度: 99.07%

TAT TFA (YGRKKRRQRRR) 源自人免疫缺陷病毒 (HIV-1) 的转录反式激活因子 (TAT)，是一种穿透细胞的肽。TAT 可以增加异源蛋白质的产量和溶解度。

TAT TFAamp;;

TAT TFA Chemical Structure

规格 价格 是否有货 数量
1 mg ￥750 In-stock
5 mg ￥2300 In-stock
10 mg ; 询价 ;
50 mg ; 询价 ;

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TAT TFA 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

TAT TFA (YGRKKRRQRRR) is derived from the transactivator of transcription (TAT) of human immunodeficiency virus (HIV-1) and is a cell-penetrating peptide. TAT can increase the yields and the solubility of heterologous proteins[1].

体外研究
(In Vitro)

TAT (YGRKKRRQRRR) represents amino acids 47-57 of the human immunodeficiency virus type I (HIV-1) TAT protein. The positively charged protein transduction domain of the HIV-1 TAT protein (TAT-PTD; residues 47-57 of TAT) rapidly translocates across the plasma membrane of living cells. TAT (YGRKKRRQRRR; TAT-PTD) can cross the hydrophobic lipid bilayer of a cell membrane simply by passive diffusion[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1673.85

Formula

C66H119N32F3O16
Sequence Shortening

YGRKKRRQRRR
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)
溶解性数据
In Vitro:;

H2O : 50 mg/mL (29.87 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.5974 mL 2.9871 mL 5.9743 mL
5 mM 0.1195 mL 0.5974 mL 1.1949 mL
10 mM 0.0597 mL 0.2987 mL 0.5974 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Ziegler A, et al. Interaction of the protein transduction domain of HIV-1 TAT with heparan sulfate: binding mechanism and thermodynamic parameters. Biophys J. 2004 Jan;86(1 Pt 1):254-63.

Tat-beclin 1

发表于由

Tat-beclin 1; 纯度: 99.68%

Tat-beclin 1 是一种自噬蛋白区域 (beclin 1) 衍生的肽，是自噬 (autophagy) 的有效诱导剂，并与自噬的负调控因子 GAPR-1 (GLIPR2) 相互作用。Tat-beclin 1 减少了体外聚谷氨酰胺扩增蛋白聚集物的积累和多种病原体(包括 HIV-1) 的复制，并降低了感染基孔肯雅病 (CHIKV) 或西尼罗河病毒 (WNV) 的小鼠的死亡率。

Tat-beclin 1amp;;

Tat-beclin 1 Chemical Structure

CAS No. : 1423821-88-8

规格 价格 是否有货 数量
1 mg ￥1000 In-stock
5 mg ￥2200 In-stock
10 mg ￥3700 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Tat-beclin 1 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Anti-Infection Compound Library
  • Antiviral Compound Library
  • Autophagy Compound Library
  • Peptide Library

生物活性

Tat-beclin 1, a peptide derived from a region of the autophagy protein (beclin 1), is a potent inducer of autophagy and interacts with negative regulator of autophagy, GAPR-1 (GLIPR2). Tat-beclin 1 decreases the accumulation of polyglutamine expansion protein aggregates and the replication of several pathogens (including HIV-1) in vitro, and reduces mortality in mice infected with chikungunya (CHIKV) or West Nile virus (WNV)[1].

IC50 Target[1]

HIV-1

;

体外研究
(In Vitro)

Tat-beclin 1 (10, 30, 50 μM; 24 hours) induces autophagy and results in a dose-dependent decrease in amounts of p62, a selective autophagy substrate, and a dose-dependent conversion of the non-lipidated form of LC3, LC3-I, to the lipidated, autophagosome-associated form of LC3, LC3-II, in multiple cell lines and primary murine embryonic fibroblasts (MEFs)[1].
Tat-beclin 1 (10 μM; 2-4 hours post-infection) decreases the intracellular survival of L. monocytogenes in primary murine bone-marrow-derived macrophages (BMDMs)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究
(In Vivo)

Tat-beclin 1 (15 mg/kg; i.p.; daily; beginning 1 day post-infection for 20 days) can induce autophagy in peripheral tissues in adult mice as well as in the central nervous system of neonatal mice (6-week-old GFP-LC3 mice)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

3741.10

Formula

C164H251N57O45
CAS 号

1423821-88-8
Sequence

Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Gly-Gly-Thr-Asn-Val-Phe-Asn-Ala-Thr-Phe-Glu-Ile-Trp-His-Asp-Gly-Glu-Phe-Gly-Thr
Sequence Shortening

YGRKKRRQRRRGGTNVFNATFEIWHDGEFGT
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

H2O : 25 mg/mL (6.68 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.2673 mL 1.3365 mL 2.6730 mL
5 mM 0.0535 mL 0.2673 mL 0.5346 mL
10 mM — — —

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Sanae Shoji-Kawata, et al. Identification of a Candidate Therapeutic Autophagy-Inducing Peptide. Nature. 2013 Feb 14;494(7436):201-6.

TAT peptide TFA

发表于由

TAT peptide TFA; 纯度: 99.60%

TAT peptide (TFA) 是一种可渗透细胞的肽 (GRKKRRQRRRPQ)，来自HIV-1反转录激活因子 (Tat)。

TAT peptide TFAamp;;

TAT peptide TFA Chemical Structure

规格 价格 是否有货 数量
1 mg ￥2500 In-stock
5 mg ￥6000 In-stock
10 mg ￥10500 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

TAT peptide TFA 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

TAT peptide (TFA) is a cell penetrating peptide (GRKKRRQRRRPQ) derived from the trans-activating transcriptional activator (Tat) from HIV-1[1][2].

体外研究
(In Vitro)

TAT peptide (TFA) is a cell penetrating peptide (GRKKRRQRRRPQ) derived from the trans-activating transcriptional activator (Tat) from HIV-1[1]. TAT peptide (TFA) (GRKKRRQRRRPQ) functionalized hybrid nanoparticles are also studied due to their combined magnetic enrichment and optical detection for cell separation and rapid cell labelling. A cell viability assay reveals good biocompatibility of these hybrid nanoparticles[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1735.93

Formula

C67H125F3N34O17
Sequence Shortening

GRKKRRQRRRPQ
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture and light

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Orzáez M, et al. Intrinsic caspase-8 activation mediates sensitization of erlotinib-resistant tumor cells toerlotinib/cell-cycle inhibitors combination treatment. Cell Death Dis. 2012 Oct 25;3:e415.

    [2]. Lou L, et al. Functionalized magnetic-fluorescent hybrid nanoparticles for cell labelling. Nanoscale. 2011 May;3(5):2315-23.

TAT peptide

发表于由

TAT peptide;

TAT peptide 是一种可渗透细胞的肽 (GRKKRRQRRRPQ)，来自HIV-1反转录激活因子 (Tat)。

TAT peptideamp;;

TAT peptide Chemical Structure

规格 价格 是否有货
1 mg ￥1200 询问价格 货期
5 mg ￥4800 询问价格 货期
10 mg ￥8100 询问价格 货期

* Please select Quantity before adding items.

TAT peptide 的其他形式现货产品:

TAT peptide TFA

生物活性

TAT peptide is a cell penetrating peptide (GRKKRRQRRRPQ) derived from the trans-activating transcriptional activator (Tat) from HIV-1[1][2].

体外研究
(In Vitro)

TAT peptide is a cell penetrating peptide (GRKKRRQRRRPQ) derived from the trans-activating transcriptional activator (Tat) from HIV-1[1]. TAT peptide (GRKKRRQRRRPQ) functionalized hybrid nanoparticles are also studied due to their combined magnetic enrichment and optical detection for cell separation and rapid cell labelling. A cell viability assay reveals good biocompatibility of these hybrid nanoparticles[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1621.91

Formula

C65H124N34O15
Sequence

Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Pro-Gln
Sequence Shortening

GRKKRRQRRRPQ
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Orzáez M, et al. Intrinsic caspase-8 activation mediates sensitization of erlotinib-resistant tumor cells toerlotinib/cell-cycle inhibitors combination treatment. Cell Death Dis. 2012 Oct 25;3:e415.

    [2]. Lou L, et al. Functionalized magnetic-fluorescent hybrid nanoparticles for cell labelling. Nanoscale. 2011 May;3(5):2315-23.

TAT (48-57)

发表于由

TAT (48-57); 纯度: 98.82%

TAT (48-57) 是可渗透细胞膜的一种多肽，来源于转录蛋白的 HIV-1 反式激活因子 (Tat) 的第 48-57 位氨基酸残基。

TAT (48-57)amp;;

TAT (48-57) Chemical Structure

CAS No. : 253141-50-3

规格 价格 是否有货 数量
1 mg ￥800 In-stock
5 mg ￥1800 In-stock
10 mg ￥2800 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

TAT (48-57) 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Anti-Infection Compound Library
  • Peptide Library

生物活性

TAT (48-57) is a cell-permeable peptide, derived from HIV-1 transactivator of transcription (Tat) protein residue 48-57.

体外研究
(In Vitro)

TAT (48-57) is a cell-permeable peptide with short length, good at crossing cell membranes of different cell types, with overall low toxicity, and does not leak out from cells once internalised[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1396.65

Formula

C55H109N31O12
CAS 号

253141-50-3
Sequence Shortening

GRKKRRQRRR
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

DMSO : 50 mg/mL (35.80 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.7160 mL 3.5800 mL 7.1600 mL
5 mM 0.1432 mL 0.7160 mL 1.4320 mL
10 mM 0.0716 mL 0.3580 mL 0.7160 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (1.79 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (1.79 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (1.79 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (1.79 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
  • 3.

    请依序添加每种溶剂：;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.5 mg/mL (1.79 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (1.79 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Cardozo AK, et al. Cell-permeable peptides induce dose- and length-dependent cytotoxic effects. Biochim Biophys Acta. 2007 Sep;1768(9):2222-34.

TAT-14

发表于由

TAT-14; 纯度: 98.43%

TAT-14 是一种 14 聚体的肽，作为 Nrf2 激活因子，具有抗炎活性。TAT-14 对 Nrf2 mRNA 表达无影响，但通过靶向 Nrf2 上Keap1 的结合位点而提高 Nrf2 蛋白水平。

TAT-14amp;;

TAT-14 Chemical Structure

CAS No. : 1362661-34-4

规格 价格 是否有货 数量
1 mg ￥3500 In-stock
5 mg ￥10500 In-stock
10 mg ￥16800 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

TAT-14 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

TAT-14 is a 14-mer peptide that acts as Nrf2 activator with an anti-inflammatory effect. TAT-14 has no effect on Nrf2 mRNA expression, but increases Nrf2 protein level due to targeting the Nrf2 binding site on Keap1[1].

IC50 Target

IC50: Nrf2[1]
分子量

3173.59

Formula

C137H230N48O39
CAS 号

1362661-34-4
Sequence

Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Leu-Gln-Leu-Asp-Glu-Glu-Thr-Gly-Glu-Phe-Leu-Pro-Ile-Gln
Sequence Shortening

YGRKKRRQRRRLQLDEETGEFLPIQ
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

H2O : 100 mg/mL (31.51 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.3151 mL 1.5755 mL 3.1510 mL
5 mM 0.0630 mL 0.3151 mL 0.6302 mL
10 mM 0.0315 mL 0.1576 mL 0.3151 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Richard Steel, et al. Anti-inflammatory Effect of a Cell-Penetrating Peptide Targeting the Nrf2/Keap1 Interaction. ACS Med Chem Lett. 2012 May 10;3(5):407-410.

Cys-TAT(47-57)(Synonyms: Cys-[HIV-Tat (47-57)])

发表于由

Cys-TAT(47-57);(Synonyms: Cys-[HIV-Tat (47-57)])

Cys-TAT(47-57) (Cys-[HIV-Tat (47-57)]) 是能穿透细胞的富含精氨酸的十二肽，是 HIV-1 反式激活蛋白衍生物。

Cys-TAT(47-57)amp;;(Synonyms: Cys-[HIV-Tat (47-57)])

Cys-TAT(47-57) Chemical Structure

CAS No. : 583836-55-9

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

Cys-TAT(47-57) (Cys-[HIV-Tat (47-57)]) is an arginine rich cell penetrating peptide derived from the HIV-1 transactivating protein.

分子量

1661.99

Formula

C67H124N34O14S
CAS 号

583836-55-9
Sequence Shortening

CYGRKKRRQRRR-NH2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Bertrand SJ, et al. HIV-1 Tat protein variants: critical role for the cysteine region in synaptodendritic injury. Exp Neurol. 2013 Oct;248:228-35.

sgp91 ds-tat Peptide 2, scrambled

发表于由

sgp91 ds-tat Peptide 2, scrambled;

sgp91 ds-tat Peptide 2, scrambled 是 NADPH 氧化酶抑制剂 gp91d –tat 肽的杂乱序列。

sgp91 ds-tat Peptide 2, scrambledamp;;

sgp91 ds-tat Peptide 2, scrambled Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

sgp91 ds-tat Peptide 2, scrambled is a scrambled sequence of NADPH oxidase inhibitor gp91 ds-tat peptide[1].

分子量

2453.00

Formula

C98H190N50O22S
Sequence Shortening

RKKRRQRRRCLRITRQSR-NH2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Lopes N, et al. Thrombospondin 2 regulates cell proliferation induced by Rac1 redox-dependent signaling. Mol Cell Biol. 2003 Aug;23(15):5401-8.