Tectochrysin(Synonyms: Techtochrysin; NSC 80687)

天然产物 黄酮类 Flavonoids

Tectochrysin (Synonyms: Techtochrysin; NSC 80687) 纯度: 99.88%

Tectochrysin (Techtochrysin) 是 Alpinia oxyphylla 的主要黄酮类化合物之一。 Tectochrysin 抑制 NF-κB 活性。

Tectochrysin(Synonyms: Techtochrysin;  NSC 80687)

Tectochrysin Chemical Structure

CAS No. : 520-28-5

规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ¥4130 In-stock
1 mg ¥1400 In-stock
5 mg ¥2300 In-stock
10 mg   询价  
50 mg   询价  

* Please select Quantity before adding items.

Tectochrysin 相关产品

相关化合物库:

  • Natural Product Library Plus
  • Bioactive Compound Library Plus
  • Immunology/Inflammation Compound Library
  • NF-κB Signaling Compound Library
  • Stem Cell Signaling Compound Library
  • Natural Product Library
  • Anti-Cancer Compound Library
  • Anti-Aging Compound Library
  • Antioxidants Compound Library
  • Differentiation Inducing Compound Library
  • Oxygen Sensing Compound Library
  • Medicine Food Homology Compound Library
  • Phenols Library
  • Pyroptosis Compound Library
  • Traditional Chinese Medicine Monomer Library
  • Flavonoids Library
  • Anti-Breast Cancer Compound Library
  • Anti-Pancreatic Cancer Compound Library
  • Anti-Blood Cancer Compound Library
  • Anti-Obesity Compound Library
  • Transcription Factor Targeted Library
  • Food-Sourced Compound Library
  • Targeted Diversity Library
  • Anti-Liver Cancer Compound Library

生物活性

Tectochrysin (Techtochrysin) is one of the major flavonoids of Alpinia oxyphylla Miquel. Tectochrysin inhibits activity of NF-κB.

IC50 & Target[1]

p50

 

p65

 

体外研究
(In Vitro)

Tectochrysin (Techtochrysin) inhibits activity of NF-κB. Tectochrysin (Techtochrysin) binds directly to the p50 unit. Tectochrysin (Techtochrysin) concentration-dependently inhibits the translocation of p50 and p65 into the nucleus through inhibition of the phosphorylation of IκB. To assess the inhibitory effect of Tectochrysin on cell growth of colon cancer cells (SW480, HCT116), cell viability is analyzed by MTT assay. The cells are treated with varying concentrations of Tectochrysin (Techtochrysin) (1, 5, 10 μg/mL) for 24 h. Tectochrysin (Techtochrysin) inhibits cell growth in colon cancer cells in a concentration-dependent manner. Tectochrysin (Techtochrysin) inhibits SW480 cells growth with IC50 value of 6.3 μg/mL and HCT116 cells growth with IC50 value of 8.4 μg/mL. Morphologic observation shows that the cells are reduced in size by the treatment of NSC 80687 (10 μg/mL) in SW480 cells and HCT116 cells. However, Tectochrysin (Techtochrysin) is not cytotoxic in the normal CCD-18co cells in the tested concentration by MTT assay. To delineate whether the induction of apoptotic cell death is critical for cell growth inhibition by NSC 80687, changes are evaluated in the chromatin morphology of cells using DAPI staining. To further characterize the apoptotic cell death by Tectochrysin (Techtochrysin), TUNEL staining assays are performed, and then the labeled cells are analyzed by fluorescence microscopy. Apoptotic cells number (DAPI-positive TUNEL stained cells) in SW480 cell is increased to 1 and 58 % by 0 and 10 μg/mL NSC 80687, respectively, and 1 and 54 % by 0 and 10 μg/mL Tectochrysin in HCT116[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

To elucidate the antitumor effects of Tectochrysin in in vivo, the tumor growth in colon cancer xenograft-bearing nude mice following Tectochrysin (NSC 80687) treatments is investigated. In HCT116 xenograft studies, Tectochrysin (NSC 80687) is administrated i.p. twice per week for 3 weeks to mice with tumors ranging from 200 to 300 mm3 in volume. The mice are weighed twice per week. The changes in body weights between the control and the Tectochrysin (NSC 80687)-treated mice (n=10) are not remarkably different during the experiment. However, On day 21, the final tumor weights are recorded. Tumor weights and volumes in mice treated with Tectochrysin (NSC 80687) at 5 mg/kg doses are 57.9 % and 46.4 % of the vehicle group, respectively[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

268.26

Formula

C16H12O4

CAS 号

520-28-5

中文名称

柚木柯因

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
In Vitro: 

DMSO : 10 mg/mL (37.28 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.7277 mL 18.6386 mL 37.2773 mL
5 mM 0.7455 mL 3.7277 mL 7.4555 mL
10 mM 0.3728 mL 1.8639 mL 3.7277 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    90% corn oil

    Solubility: ≥ 1.67 mg/mL (6.23 mM); Clear solution

    此方案可获得 ≥ 1.67 mg/mL (6.23 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 16.7 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 Shanghai Jinpan Biotech Co Ltd 网站选购。
参考文献
  • [1]. Park MH, et al. Anticancer effect of Tectochrysin in colon cancer cell via suppression of NF-kappaB activity and enhancement of death receptor expression. Mol Cancer. 2015 Jun 30;14:124.

Cell Assay
[1]

Each SW480, HCT116, HT-29, A549 and MCF-7 cell line (1×104 cells) is incubated in 200 μL of RPMI 1640, DMEM medium with NSC 80687 (concentrations ranging from 1, 5, 10 μg/mL) in a 96-well flat-bottomed plate in triplicate. After incubation for 72 h at 37°C, MTT diluted in RPMI 1640, DMEM medium are added to each well and incubation is carried out for 90 min. The supernatant is then discarded and the crystal products are eluted with DMSO (200 μL/well). Colorimetric evaluation is performed with a spectrophotometer at 540 nm. The apoptosis assay is first performed by using DAPI staining. SW480 and HCT116 human colon cancer cells are cultured with concentrations of NSC 80687 (5 μg/mL), and induction of apoptotic cell death is evaluated after 24 h. Tunel assay is done[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

Mice[1]
Five-week-old male BALB/c athymic nude mice (n=10/group) are used. HCT116 cancer cells are injected subcutaneously (1×107 cells/0.1 mL PBS/animal) into the lower right flanks of mice. After 14 days, when the tumors have reached an average volume of 200-300 mm3, the tumor-bearing nude mice are intraperitoneally injected with Tectochrysin (NSC 80687) (5 mg/kg dissolved in 0.1 % DMSO) twice per week for 3 weeks. The tumor volumes are measured with vernier calipers and calculated[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Park MH, et al. Anticancer effect of Tectochrysin in colon cancer cell via suppression of NF-kappaB activity and enhancement of death receptor expression. Mol Cancer. 2015 Jun 30;14:124.