Thiazole orange is an unsymmetrical cyanine dye which can be conjugated to oligonucleotides (ONs) to create fluorogenic hybridisation probes. Thiazole orange can be used for reticulocyte analysis[1][2].
分子量
476.61
Formula
C26H24N2O3S2
CAS 号
107091-89-4
中文名称
噻唑橙
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
4deg;C, sealed storage, away from moisture and light
*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:;
DMSO : 25 mg/mL (52.45 mM; Need ultrasonic)
H2O : < 0.1 mg/mL (insoluble)
配制储备液
浓度溶剂体积质量
1 mg
5 mg
10 mg
1 mM
2.0982 mL
10.4908 mL
20.9815 mL
5 mM
0.4196 mL
2.0982 mL
4.1963 mL
10 mM
0.2098 mL
1.0491 mL
2.0982 mL
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献
[1]. Linda G. Le, et al. Thiazole Orange: A New Dye for Reticulocyte Analysis. cytometry 7:508-517 (1986)
[2]. Piotr Klimkowski, et al. Design of thiazole orange oligonucleotide probes for detection of DNA and RNA by fluorescence and duplex melting. Org Biomol Chem. 2019 Jun 28; 17(24): 5943–5950.