Thiazolyl Blue (MTT) 是一种广泛用于测量细胞增殖的比色剂。Thiazolyl Blue (MTT) 在活细胞中从黄色还原为紫色甲。
Thiazolyl Blue Chemical Structure
CAS No. : 298-93-1
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Free Sample (0.1-0.5 mg)
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10;mM;*;1 mL in DMSO
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500 mg
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1 g
¥800
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5 g
¥3000
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10 g
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Thiazolyl Blue 相关产品
bull;相关化合物库:
Bioactive Compound Library Plus
生物活性
Thiazolyl Blue (MTT) is a colorimetric agent widely used to measure cell proliferation. Thiazolyl Blue (MTT) is reduced from yellow color to purple formazan in living cells.
体外研究 (In Vitro)
Thiazolyl Blue (MTT) is used in conjunction with rhodamine B to measure mitochondrial membrane potential. Thiazolyl Blue-formazan is generated in mitochondria acts as a fluorescence quencher for rhodamine that distributes across membranes of viable cells according to membrane potential. In the absence of mPMS, cellular reduction of Thiazolyl Blue is strong. Thiazolyl Blue is membrane-impermeable when incorporated into large unilamellar liposomes, and therefore it is taken up by cells via endocytosis[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
414.32
Formula
C18H16BrN5S
CAS 号
298-93-1
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
4deg;C, sealed storage, away from moisture and light
*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:;
DMSO : 25 mg/mL (60.34 mM; ultrasonic and warming and heat to 60°C)
H2O : 0.88 mg/mL (2.12 mM; Need ultrasonic and warming)
配制储备液
浓度溶剂体积质量
1 mg
5 mg
10 mg
1 mM
2.4136 mL
12.0680 mL
24.1359 mL
5 mM
0.4827 mL
2.4136 mL
4.8272 mL
10 mM
0.2414 mL
1.2068 mL
2.4136 mL
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂: