Mito-TRFS, the first off-on probe, is used to image the mitochondrial thioredoxin reductase (TrxR2) in live cells[1].
体外研究 (In Vitro)
Mito-TRFS (10 μM) has the maximal absorption at ~375 nm in TE buffer (50 mM Tris-HCl and 1 mM EDTA, pH 7.4) at 37 °C[1]. Mito-TRFS itself has a strong emission at ~480 nm when excited at 375 nm in TE buffer (φ=0.34), but has weak emission when excited at 438 nm[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Immunofluorescence[1].
Cell Line:
HeLa cells.
Concentration:
1 μM.
Incubation Time:
2 h.
Result:
Mito-TRFS is localized predominantly in the mitochondria of live cells.
分子量
820.70
Formula
C38H34IN2O5PS2
CAS 号
1859102-62-7
运输条件
Room temperature in continental US; may vary elsewhere.
[1]. Yaping Liu, et al. A small molecule probe reveals declined mitochondrial thioredoxin reductase activity in a Parkinson’s disease model. Chem Commun (Camb). 2016 Feb 7;52(11):2296-9.
Mito-TRFS, the first off-on probe, is used to image the mitochondrial thioredoxin reductase (TrxR2) in live cells[1].
体外研究 (In Vitro)
Mito-TRFS (10 μM) has the maximal absorption at ~375 nm in TE buffer (50 mM Tris-HCl and 1 mM EDTA, pH 7.4) at 37 °C[1]. Mito-TRFS itself has a strong emission at ~480 nm when excited at 375 nm in TE buffer (φ=0.34), but has weak emission when excited at 438 nm[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Immunofluorescence[1].
Cell Line:
HeLa cells.
Concentration:
1 μM.
Incubation Time:
2 h.
Result:
Mito-TRFS is localized predominantly in the mitochondria of live cells.
分子量
820.70
Formula
C38H34IN2O5PS2
CAS 号
1859102-62-7
运输条件
Room temperature in continental US; may vary elsewhere.
[1]. Yaping Liu, et al. A small molecule probe reveals declined mitochondrial thioredoxin reductase activity in a Parkinson’s disease model. Chem Commun (Camb). 2016 Feb 7;52(11):2296-9.
Fast-TRFS is a selective and superfast fluorogenic probe of thioredoxin reductase (TrxR). Fast-TRFS can be used for imaging TrxR activity in live cells[1].
体外研究 (In Vitro)
Fast-TRFS reaches the maximal fluorescence signal within 1 min incubated with TCEP, and within 5 min incubated with the TrxR enzyme[1]. Fast-TRFS (10 μM; 2-60 min) appears the blue fluorescence signal within 2 min in HeLa cells[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
376.37
Formula
C14H11F3N2O3S2
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Powder
-20deg;C
3 years
4deg;C
2 years
In solvent
-80deg;C
6 months
-20deg;C
1 month
参考文献
[1]. Li X, et, al. A fast and specific fluorescent probe for thioredoxin reductase that works via disulphide bond cleavage. Nat Commun. 2019 Jun 21;10(1):2745.
TRFS-red, a red fluorescence emission off-on probe, is selective for thioredoxin reductase (TrxR). TRFS-red exhibits high response rate and sensitivity. TRFS-red can be used for imaging live cells[1].
体外研究 (In Vitro)
TRFS-red (1 μM; 30-120 min) gives red fluorescence signal in live HeLa cells, and the red fluorescence is predominantly distributed in the cytosol[1]. TRFS-red (10 μM) has a maximal absorption at ~530 nm in TE buffer (50 mM Tris-HCl and 1 mM EDTA, pH 7.4)[1]. TRFS-red displays a reliable fluorescence signal in the pH range of 5.5-8.5[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
682.12
Formula
C28H28ClN3O11S2
运输条件
Room temperature in continental US; may vary elsewhere.
