Suc-Leu-Leu-Val-Tyr-AMC; 纯度: 98.87%
Suc-Leu-Leu-Val-Tyr-AMC 是一种荧光底物。

Suc-Leu-Leu-Val-Tyr-AMC Chemical Structure
CAS No. : 94367-21-2
规格 | 价格 | 是否有货 | 数量 |
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1 mg | ¥660 | In-stock | |
5 mg | ¥1300 | In-stock | |
10 mg | ; | 询价 | ; |
50 mg | ; | 询价 | ; |
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Suc-Leu-Leu-Val-Tyr-AMC 相关产品
bull;相关化合物库:
- Bioactive Compound Library Plus
- Peptide Library
生物活性 |
Suc-Leu-Leu-Val-Tyr-AMC is a fluorogenic substrate. |
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体外研究 (In Vitro) |
Suc-Leu-Leu-Val-Tyr-AMC (Suc-LLVY) is a membrane-permeable calpain-specific fluorogenic substrate, pteolytic hydrolysis of the peptidyl-7-amino bond liberates the highly fluorescent 7-amino-4-methylcoumarin (AMC) moiety[1]. The effect of TGF-β on hydrolysis of these substrates (e.g Suc-Leu-Leu-Val-Tyr-AMC) are assessed. Biliary epithelial H69 cells are incubated with 10, 1, 0.1, or 0 ng/mL TGF-β for 24 h. Substrate hydrolysis is then fluorometrically assessed in cytosolic extracts. Basal activity is 1.12, 8.33, and 14.52 nmol AMC/mg protein/min for suc-LLVY-AMC, z-LLE-AMC, and z-LLL-AMC hydrolysis, respectively[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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分子量 |
763.88 |
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Formula |
C40H53N5O10 |
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CAS 号 |
94367-21-2 |
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Sequence |
Suc-Leu-Leu-Val-Tyr |
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Sequence Shortening |
Suc-LLVY |
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运输条件 |
Room temperature in continental US; may vary elsewhere. |
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储存方式 |
Protect from light, stored under nitrogen
*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light, stored under nitrogen) |
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溶解性数据 |
In Vitro:;
DMSO : ≥ 20 mg/mL (26.18 mM) * “≥” means soluble, but saturation unknown. 配制储备液
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
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参考文献 |
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Kinase Assay [3] |
Immunoprecipitation is carried out for the two sets of samples, using the same amount of protein. The 20 S and 26 S proteasome immunoprecipitates are washed with 50 mM Hepes/KOH (pH 7.5), and 50 mM Hepes/KOH (pH 7.5) containing 2 mMATP, respectively, prior to the determination of peptidase activity using 50 μM suc-Leu- Leu-Val-Tyr-AMC as substrate in these buffers[3]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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参考文献 |
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