荧光染料NEP(Synonyms: VDP-green (NEP))

荧光染料Dye Reagents NEP;(Synonyms: VDP-green (NEP))

NEP (VDP-green (NEP)) 是一种基于分子内电荷转移 (ICT) 机制的感应荧光探针,用于感应含邻二硫醇的蛋白质 (VDPs)。NEP 在活细胞和动物体内对 VDPs 表现出高选择性,并显示强绿色荧光信号 (λexem=430/535 nm)。NEP 具有用于帕金森氏症研究的潜力。

NEP(Synonyms: VDP-green (NEP))

NEP Chemical Structure

CAS No. : 2414276-32-5

规格 价格 是否有货
5 mg ¥3500 询问价格 货期
10 mg ¥5800 询问价格 货期

* Please select Quantity before adding items.

生物活性

NEP (VDP-green (NEP)) is a turn-on fluorescent probe based on the intramolecular charge transfer (ICT) mechanism for sensing vicinal dithiol-containing proteins (VDPs). NEP exhibits high selectivity toward VDPs in live cells and in vivo and displays a strong green fluorescence signal (λexem=430/535 nm). NEP has the potential for parkinsonism[1].

体外研究
(In Vitro)

NEP (1-30 μM; 6 hours) has no or little cytotoxicity in HepG2 and PC12 cells[1].
NEP (10 μM; 4 hours) causes the fluorescence intensity to decrease gradually in PC12 cells pretreated with 6-OHDA (0, 50, 100, and 200 μM; for 30 min)[1].
NEP contains a dithiarsolane moiety (five-membered As-S ring) as the receptor of VDPs. In the presence of VDPs, NEP displays a strong green fluorescence signal produced by the cyclic dithiarsolane cleavage and subsequent intramolecular cyclization to liberate the fluorophore. NEP maintains a reliable fluorescence response within the range of pH 7-10[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

NEP (10 μM; for 4 h) causes the obvious green signal in zebrafishes (4 day old)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

分子量

654.63

Formula

C29H31AsN4O5S2

CAS 号

2414276-32-5

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Guodong Hu, et al. Decrease of Protein Vicinal Dithiols in Parkinsonism Disclosed by a Monoarsenical Fluorescent Probe. Anal Chem. 2020 Mar 17;92(6):4371-4378.

荧光染料AC-green(Synonyms: VDP-green)

荧光染料Dye Reagents AC-green;(Synonyms: VDP-green)

AC-green (VDP-green) 是一种 β-烯丙基氨基甲酸酯荧光探针,可选择性地对活体系统中的邻位二硫醇蛋白质 (VDPs) 进行成像 (λex/λem=400/475 nm)。AC-green 可以高灵敏度检测降低牛血清白蛋白 (rBSA)。AC-green 毒性低,灵敏度高,适用于检测活细胞和斑马鱼中的 VDP。

AC-green(Synonyms: VDP-green)

AC-green Chemical Structure

规格 价格 是否有货
5 mg ¥3500 询问价格 货期
10 mg ¥5800 询问价格 货期

* Please select Quantity before adding items.

生物活性

AC-green (VDP-green) is a β-allyl carbamate fluorescent probe for specifically imaging vicinal dithiol proteins (VDPs) in living systems (λexem=400/475 nm). AC-green can detect the reduced bovine serum albumin (rBSA) with high sensitivity. AC-green displays low toxicity and features high sensitivity, and is suitable for sensing VDPs in living cells and zebrafishes[1].

体外研究
(In Vitro)

AC-green (VDP-green) can respond to VDPs with more than 60-fold increase of emission in aqueous solution, while there is no significant interference from biological thiols, amino acids or inorganic salts[1].
AC-green (2 μM; 90 min) has no apparent fluorescence signal within the pH range of 5.0-9.0. Addition of rBSA turns on the fluorescence[1].
AC-gree (10 μM) has low cytotoxicity in HepG2 cells and Hela cells[1].
AC-gree (10 μM; for 15 min) images VDPs in living HepG2 cells and bright green fluorescence appeared. This fluorescence is inhibited when the cells are pretreated with PAO, a popular specificligand for protein vicinal dithiols[1].
AC-gree (10 μM; for 20 min) incubates with zebrafishes has a strong fluorescence signal appeared in the green channel[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

435.47

Formula

C24H25N3O5

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Lanning Zhao, et al. A β-allyl carbamate fluorescent probe for vicinal dithiol proteins. Chem Commun (Camb). 2020 Mar 5;56(19):2857-2860.