Sulforhodamine B sodium salt(Synonyms: Acid Red 52 Kiton Red 620)

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Sulforhodamine B sodium salt;(Synonyms: Acid Red 52; Kiton Red 620)

Sulforhodamine B sodium salt 是一种荧光染料，可用于定量测定培养细胞的细胞蛋白量。

Sulforhodamine B sodium saltamp;;(Synonyms: Acid Red 52; Kiton Red 620)

Sulforhodamine B sodium salt Chemical Structure

CAS No. : 3520-42-1

规格 价格 是否有货
10;mM;*;1 mL in DMSO ￥550 询问价格 货期
100 mg ￥500 询问价格 货期
1 g ￥700 询问价格 货期

* Please select Quantity before adding items.

生物活性

Sulforhodamine B sodium salt is a fluorescent dye with uses spanning from laser-induced fluorescence (LIF) to the quantification of cellular proteins of cultured cells.

体外研究
(In Vitro)

Sulforhodamine B (SRB) is often used as a membrane-impermeable polar tracer or used for cell density determination via determination of cellular proteins (cytotoxicity assay). The SRB assay has been used to inexpensively conduct various screening assays to investigate cytotoxicity in cell based studies. This method relies on the property of SRB, which binds stoichiometrically to proteins under mild acidic conditions and then can be extracted using basic conditions; thus, the amount of bound dye can be used as a proxy for cell mass, which can then be extrapolated to measure cell proliferation. The protocol can be divided into four main steps: preparation of treatment, incubation of cells with treatment of choice, cell fixation and SRB staining, and absorbance measurement. This assay is limited to manual or semiautomatic screening, and can be used in an efficient and sensitive manner to test chemotherapeutic drugs or small molecules in adherent cells. It also has applications in evaluating the effects of gene expression modulation (knockdown, gene expression upregulation), as well as to study the effects of miRNA replacement on cell proliferation[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

580.65

Formula

C27H29N2NaO7S2
CAS 号

3520-42-1
中文名称

磺酰罗丹明 B；柴林红 B；奇通红 S；酸性桃红 B；酸性红 52；二甲苯红 B；酸性玫瑰红 B；磺化罗丹明 B；硫罗丹明 B；食品红 106
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:;

DMSO : 50 mg/mL (86.11 mM; Need ultrasonic)

H2O : 20 mg/mL (34.44 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.7222 mL 8.6110 mL 17.2221 mL
5 mM 0.3444 mL 1.7222 mL 3.4444 mL
10 mM 0.1722 mL 0.8611 mL 1.7222 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Orellana EA, et al. Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation. Bio Protoc. 2016 Nov 5;6(21). pii: e1984.
Cell Assay
[1]

Gently add 25 μL (96-well format) or 5 μL (384-well format) cold 50% (wt/vol) TCA to each well directly to medium supernatant, and incubate the plates at 4 °C for 1 h. Mixing is not required, as this could lead to some cells detaching from the bottom of the well. Wash the plates four times by submerging the plate in a tub with slow-running tap water and remove excess water by gently tapping the plate into a paper towel. After the last wash allow the plate to air-dry at room temperature. Add 50 μL (96-well format) or 20 μL (384-well format) of 0.04% (wt/vol) SRB solution to each well. Leave at room temperature for 1 h and then quickly rinse the plates four times with 1% (vol/vol) acetic acid (200 μL for 96-well format or 30 μL for 384-well format) to remove unbound dye. Allow the plate to air-dry at room temperature[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Orellana EA, et al. Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation. Bio Protoc. 2016 Nov 5;6(21). pii: e1984.