DiI(Synonyms: DiIC18(3))

DiI;(Synonyms: DiIC18(3)) 纯度: 99.92%

DiI (DiIC18(3)) 是一种亲脂性膜染料。DiI 在进入细胞膜之前荧光非常弱,仅当进入到细胞膜后才可以被激发出很强的荧光。DiI 被激发后可以发出橙红色的荧光,最大激发波长为 549 nm,最大发射波长为 565 nm。通常用作神经元和其它细胞的长期示踪剂。

DiIamp;;(Synonyms: DiIC18(3))

DiI Chemical Structure

CAS No. : 41085-99-8

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ¥605 In-stock
10 mg ¥550 In-stock
25 mg ¥1000 In-stock
50 mg ¥1500 In-stock
100 mg ; 询价 ;
200 mg ; 询价 ;

* Please select Quantity before adding items.

DiI 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

DiI (DiIC18(3)) is a lipophilic carbocyanine fluorescent dye for membrane labeling. DiI has an absorption maximum at 549 nm and an emission maximum 565 nm. DiI is mildly fluorescent in aqueous suspension, but becomes bright when bound to cell membrane.

体外研究
(In Vitro)

Once bound to a membrane it diffuses laterally in 2 dimensions; if there is no diffusion barrier, DiI generally stains the whole leaflet (one surface) of a biological membrane rapidly, but does not readily flip across to the other leaflet.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

DiI-labeled motoneurons have remained viable for up to 4 weeks in culture and up to one year in vivo[1]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

933.87

Formula

C59H97ClN2O4

CAS 号

41085-99-8

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)

溶解性数据
In Vitro:;

DMSO : 12.5 mg/mL (13.39 mM; ultrasonic and warming and heat to 80°C)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.0708 mL 5.3541 mL 10.7081 mL
5 mM 0.2142 mL 1.0708 mL 2.1416 mL
10 mM 0.1071 mL 0.5354 mL 1.0708 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: 1.25 mg/mL (1.34 mM); Suspended solution; Need ultrasonic

    此方案可获得 1.25 mg/mL (1.34 mM) 的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例,取 100 μL 12.5 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: 1.25 mg/mL (1.34 mM); Suspended solution; Need ultrasonic

    此方案可获得 1.25 mg/mL (1.34 mM) 的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例,取 100 μL 12.5 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. D P Kuffler, et al. Long-term survival and sprouting in culture by motoneurons isolated from the spinal cord of adult frogs. J Comp Neurol. 1990 Dec 22;302(4):729-38.

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