millipore转印膜IPVH08130 IPVH08130


millipore转印膜IPVH08130

millipore转印膜IPVH08130 Millipore 提供四种不同的不动 PVDF 膜,每种膜都针对不同的蛋白质印迹应用进行优化。 millipore转印膜IPVH08130 孔径0.45 微米

 millipore转印膜IPVH08130 

Millipore 提供三种不同的Immobilon PVDF膜,是针对不同的蛋白质印迹应用的理想选择。现在我们还提供采用按规格裁切的印迹方片膜和两层过滤纸组成的印迹三明治。

与硝酸纤维素膜相比,Immobilon膜具有多种优点,它们不易破裂或卷曲,便于裁剪且在切割过程中不会碎裂。它们还有低背景,广泛溶剂相容性以及优异的着色能力。此外,它们可以重复检测。

Description
Catalogue Number IPVH08130
Trade Name
  • Immobilon
Description Immobilon-P PVDF Membrane
Background Information Overview
– Millipore offers four different Immobilon PVDF membranes, each optimized for different protein blotting applications.
Immobilon PVDF membranes provide many advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times.

Immobilon-P Transfer Membrane:
– 45 µm pore size Recommended for most western blotting, especially proteins greater than 20 kDa
– Millipore’s Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity

Immobilon-E Membrane:
– the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
– 45 µm pore size recommended for most western blotting, especially proteins greater than 20 kDa

Blotting Sandwiches:
– When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper and are compatible with any standard wet transfer device.

Blotting Filter Paper:
– Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes.

Immobilon-PSQ Membrane:
– 0.2 µm pore size and a large internal structure
– Higher protein adsorption and sequencing yields than other membranes
– Recommended for blotting proteins less than 20 kDa
– Prevents blow-through of low molecular weight proteins

Immobilon-FL Membrane:
– The first transfer membrane optimized for fluorescence applications
– Extremely low background improves sensitivity of all fluorescence detection protocols
– Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
– Ideal for multiplexing and chemifluorescence applications

Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon® PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. In addition, Immobilon® PVDF membranes optimized for fluorescent blots dramatically increase signal-to-noise ratios for high sensitivity in quantitative, multiplexing applications.

Features & Benefits:
– Won’t crack, curl or fracture when cut
– Low background
– Superior staining capabilities
– Can be reprobed multiple times

Applications:
– Western Blotting, Immunoblotting, Dot Blotting, Protein Sequencing;
– Compatible with Chemiluminescent, Fluorescent, Radioactive, Chromogenic, and Chemifluorescent Detection
– Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
References

Product Information
Detection method Chromogenic Radioactive Fluorescent Chemiluminescence
Compatible Stains
  • Coomassie brilliant blue
  • Amido black
  • India ink
  • Ponceau-S red
  • Colloidal gold
  • CPTS
  • Toluidine blue
  • Transillumination
  • Sypro® ruby
Filter Code IPVH
Filter Color White

Applications
Application 10 sheets, 8.5 cm x 13.5 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
Key Applications
  • Western Blotting

Biological Information
Primary Binding Mechanisms Electrostatic, hydrophobic
BSA Adsorption 215 µg/cm²
Goat IgG Adsorption 294 µg/cm²
Insulin Adsorption 160 µg/cm²
Wettability Hydrophobic

Physicochemical Information
Pore Size 0.45 µm

Dimensions
Filter Surface Plain
Filter Dimensions 8.5 cm x 13.5 cm

Materials Information
Chemistry
  • Hydrophobic Polyvinylidene Fluoride (PVDF)
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information

Packaging Information
Material Size 10

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